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Literature summary for 3.2.1.73 extracted from

  • Hahn, M.; Olsen, O.; Politz, O.; Borriss, R.; Heinemann, U.
    Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase (1995), J. Biol. Chem., 270, 3081-3088.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Paenibacillus macerans

Protein Variants

Protein Variants Comment Organism
D105K activity is reduced to less than 1% Paenibacillus macerans
D105N activity is reduced to less than 1% Paenibacillus macerans
E103D activity is reduced to less than 1% Paenibacillus macerans
E103Q activity is reduced to less than 1% Paenibacillus macerans
E107D activity is reduced to less than 1% Paenibacillus macerans
E107H activity is reduced to less than 1% Paenibacillus macerans
E107Q activity is reduced to less than 1% Paenibacillus macerans
W101F activity is reduced to less than 1% Paenibacillus macerans
W101Y activity is reduced to less than 1% Paenibacillus macerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information wild-type and mutant enzymes Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
-
 wild-type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H
-

Purification (Commentary)

Purification (Comment) Organism
wild type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H Paenibacillus macerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
 Paenibacillus macerans ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 lichenan mutant enzyme E107D Paenibacillus macerans
0.55
-
 lichenan mutant enzyme E103D Paenibacillus macerans
0.7
-
 lichenan mutant enzyme E107H Paenibacillus macerans
1.7
-
 lichenan mutant enzyme W101Y Paenibacillus macerans
2.9
-
 lichenan mutant enzyme W101F Paenibacillus macerans
3.1
-
 lichenan mutant enzyme D105N Paenibacillus macerans
1020
-
 lichenan wild-type enzyme Paenibacillus macerans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6 mutant enzyme E107H Paenibacillus macerans
6 7 wild-type enzyme and mutant enzymes W101F, W101Y, E103D, D105N and E107D Paenibacillus macerans