Crystallization (Comment) | Organism |
---|---|
- |
Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
D105K | activity is reduced to less than 1% | Paenibacillus macerans |
D105N | activity is reduced to less than 1% | Paenibacillus macerans |
E103D | activity is reduced to less than 1% | Paenibacillus macerans |
E103Q | activity is reduced to less than 1% | Paenibacillus macerans |
E107D | activity is reduced to less than 1% | Paenibacillus macerans |
E107H | activity is reduced to less than 1% | Paenibacillus macerans |
E107Q | activity is reduced to less than 1% | Paenibacillus macerans |
W101F | activity is reduced to less than 1% | Paenibacillus macerans |
W101Y | activity is reduced to less than 1% | Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutant enzymes | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
wild-type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H | - |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lichenan + H2O | - |
Paenibacillus macerans | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
lichenan | mutant enzyme E107D | Paenibacillus macerans | |
0.55 | - |
lichenan | mutant enzyme E103D | Paenibacillus macerans | |
0.7 | - |
lichenan | mutant enzyme E107H | Paenibacillus macerans | |
1.7 | - |
lichenan | mutant enzyme W101Y | Paenibacillus macerans | |
2.9 | - |
lichenan | mutant enzyme W101F | Paenibacillus macerans | |
3.1 | - |
lichenan | mutant enzyme D105N | Paenibacillus macerans | |
1020 | - |
lichenan | wild-type enzyme | Paenibacillus macerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | mutant enzyme E107H | Paenibacillus macerans |
6 | 7 | wild-type enzyme and mutant enzymes W101F, W101Y, E103D, D105N and E107D | Paenibacillus macerans |