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Literature summary for 3.2.1.73 extracted from

  • Elgharbi, F.; Hmida-Sayari, A.; Sahnoun, M.; Kammoun, R.; Jlaeil, L.; Hassairi, H.; Bejar, S.
    Purification and biochemical characterization of a novel thermostable lichenase from Aspergillus niger US368 (2013), Carbohydr. Polym., 98, 967-975.
    View publication on PubMed
Search for more literature for 3.2.1.73

Application

Application Comment Organism
food industry the enzyme is considered as a candidate for application particularly in the animal feed industry Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 11% inhibition at 5 mM Aspergillus niger
Co2+ 52% inhibition at 5 mM Aspergillus niger
Cu2+ complete inhibition at 5 mM Aspergillus niger
Fe2+ complete inhibition at 5 mM Aspergillus niger
Mn2+ complete inhibition at 5 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, 0.62 mg/ml with barley beta-glucan at pH 5.0, 60°C,and 0.38 mg/ml with lichenan at pH 5.0, 60°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus niger
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information poor effects by Mg2+ and Zn2+ at 5 mM Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger US368
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 14.24fold from culture supernatant by ultrafiltration and gel filtration to homogeneity Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of production of beta-glucanase with barley flour as the sole carbon source Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Aspergillus niger cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h ?
barley beta-glucan + H2O
-
 Aspergillus niger US368 cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides (mainly trisaccharide and tetrasaccharide) followed by disaccharide (cellobiose) and glucose are formed as the major products of hydrolysis after a prolonged incubation of 4 h ?
lichenan + H2O
-
 Aspergillus niger ?
-
 ?
lichenan + H2O
-
 Aspergillus niger US368 ?
-
 ?
additional information the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose Aspergillus niger ?
-
 ?
additional information the enzyme is a conformation-retaining and is only active toward glucan containing beta-1,3-1,4-linkages. No activity with laminarin and carboxymethyl cellulose Aspergillus niger US368 ?
-
 ?

Subunits

Subunits Comment Organism
? x *32000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
beta-1,3;1,4-glucanase
-
 Aspergillus niger
Lichenase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 activity range, profile overview Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, pH 5.0, half-life is 150 min Aspergillus niger
60
-
 purified enzyme, pH 5.0, half-life is 135 min Aspergillus niger
65
-
 purified enzyme, pH 5.0, half-life is 90 min Aspergillus niger
70
-
 purified enzyme, pH 5.0, half-life is 30 min Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.32
-
 lichenan pH 5.0, 60°C Aspergillus niger
19.11
-
 Barley beta-glucan pH 5.0, 60°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 activity range, profile overview Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 purified enzyme, 37°C, 1 h, completely stable Aspergillus niger