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(+)-alliin + H2O
allicin + pyruvate + NH3
-
-
-
-
?
(+)methiin + H2O
methanesulfenic acid + pyruvate + NH3
-
-
-
-
?
(+)propiin + H2O
propane-1-sulfenic acid + pyruvate + NH3
-
-
-
-
?
(+-)-alliin + H2O
allicin + pyruvate + NH3
-
-
-
-
?
(+-)-butiin + H2O
butane-1-sulfenic acid + pyruvate + NH3
-
-
-
-
?
(+-)-ethiin + H2O
ethanesulfenic acid + pyruvate + NH3
-
-
-
-
?
(+-)methiin + H2O
methanesulfenic acid + pyruvate + NH3
-
-
-
-
?
(+-)propiin + H2O
propane-1-sulfenic acid + pyruvate + NH3
-
-
-
-
?
1-amino-3-(methylthio)propylphosphinic acid + H2O
1-oxopropylphosphinic acid + methanethiol + NH3
-
-
-
?
2-amino-3-(N,N-dimethylamino)propionic acid
2-iminopropionic acid + ?
-
C-N-bond cleavage
-
?
alliin + H2O
allicin + pyruvic acid + NH3
-
-
-
-
?
beta-chloro-L-alanine + H2O
?
-
-
-
-
?
cystathionine + ?
?
-
-
-
-
?
DL-2-amino-3-(N-hydroxyethylamino)-propionic acid
ethanolamine + NH3 + pyruvate
-
-
-
?
DL-2-amino-3-(N-methylamino)propionic acid
methylamine + NH3 + pyruvate
-
-
-
?
DL-ethionine + H2O
?
-
-
-
-
?
DL-homocysteic acid + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
2-oxobutanoate + NH3 + H2S
DL-homocysteine + H2O
2-oxobutanoate + NH3 + hydrogen sulfide
-
-
-
-
?
DL-homocysteine + H2O
H2S + S-(beta-hydroxyethyl)-L-homocysteine
-
gamma-replacement reaction, wild-type enzyme
-
-
?
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
DL-homoserine + H2O
NH3 + 2-oxobutanoate
-
-
-
-
?
ethiin + H2O
ethanesulfenic acid + pyruvate + NH3
-
-
-
-
?
homocysteine + alkanethiol
S-alkylhomocysteine
-
-
corresponding
?
L-1-amino-3-methylthiopropylphosphinic acid + H2O
methanethiol + NH3 + propanoylphosphinic acid
Arg374 and Ser339 are involved in the binding of carboxyl groups of the substrate, the hydroxyl of Tyr113 is a potential acceptor of a proton from the amino groups of the amino acid
-
-
?
L-cystathionine + ?
?
-
-
-
-
?
L-cysteine
2-oxopropanoate + NH3 + H2S
-
-
-
-
?
L-cysteine + H2O
H2S + S-(beta-hydroxyethyl)-L-cysteine
-
beta-replacement reaction, wild-type enzyme and mutant C116H
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
L-cystine + H2O
?
assay at pH 8.0, 37°C
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
L-Gly(vinyl) + H2O
?
-
-
-
-
?
L-homocysteine
2-oxobutanoate + NH3 + hydrogen sulfide
L-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
L-homocysteine + H2O
sulfide + NH3 + 2-oxobutanoate
L-homoserine
NH3 + 2-oxobutanoate
-
-
-
?
L-methionine
methanethiol + NH3 + 2-oxobutanoate
L-methionine + 2-butylthiol
S-2-butylhomocysteine
-
-
-
?
L-methionine + 2-mercaptoethanol
methanethiol + S-(beta-hydroxyethyl)-L-homocysteine
-
gamma-replacement reaction, wild-type enzyme
-
-
?
L-methionine + 2-propylthiol
S-2-propylhomocysteine
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
L-methionine sulfone + H2O
? + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine sulfoxide
?
-
-
-
-
?
L-methionine sulfoxide + H2O
?
L-selenodjenkolate
pyruvate + NH3 + H2Se + formaldehyde
-
-
-
?
L-selenomethionine + H2O
?
-
-
-
-
?
methiin + H2O
methanesulfenic acid + pyruvate + NH3
-
-
-
-
?
O-acetyl-DL-homoserine + H2O
NH3 + 2-oxobutanoate + acetate
-
-
-
-
?
O-acetyl-L-homoserine
?
-
-
-
?
O-acetyl-L-homoserine + H2O
2-oxobutanoate + NH3 + acetate
-
-
-
-
?
O-acetyl-L-homoserine + H2O
?
-
-
-
-
?
O-acetyl-L-serine + H2O
2-oxopropanoate + NH3 + acetate
O-succinyl-L-homoserine + H2O
?
-
weak activity
-
-
?
S-benzyl-L-cysteine + H2O
?
S-benzyl-L-cysteine + H2O
thiobenzyl alcohol + NH3 + 2-oxopropanoate
S-butyl-L-cysteine + H2O
butanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-ethyl-L-cysteine
?
-
-
-
?
S-ethyl-L-cysteine + H2O
?
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + 2-oxopropanoate
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + pyruvate
Arg374 and Ser339 are involved in the binding of carboxyl groups of the substrate, the hydroxyl of Tyr113 is a potential acceptor of a proton from the amino groups of the amino acid. Formation of external aldimine, conformational changes in the active center enable the Tyr58 hydroxyl group to occupy a position favorable for protonation of the leaving group
-
-
?
S-ethyl-L-homocysteine + H2O
?
S-ethyl-L-homocysteine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
S-methyl-L-cysteine
?
-
-
-
?
S-methyl-L-cysteine + 2-mercaptoethanol
?
-
-
-
-
?
S-methyl-L-cysteine + H2O
?
-
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + NH3 + 2-oxopropanoate
S-methyl-L-cysteine + H2O
pyruvate + NH3 + CH3SH
S-methylcysteine + H2O
?
-
-
-
-
?
S-propyl-L-cysteine + H2O
propanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
Se-methylselenocysteine
?
selenoethionine + H2O
ethylselenol + NH3 + 2-oxobutanoate
-
-
-
?
selenomethionine + H2O
methylselenol + NH3 + 2-oxobutanoate
trifluoromethionine + H2O
?
-
-
-
-
?
trifluoromethionine + H2O
trifluoromethanethiol + NH3 + 2-oxobutanoate
-
-
-
?
DL-homocysteine + H2O

2-oxobutanoate + NH3 + H2S
-
-
-
-
?
DL-homocysteine + H2O
2-oxobutanoate + NH3 + H2S
112% of activity with L-methionine
-
?
DL-homocysteine + H2O
2-oxobutanoate + NH3 + H2S
294% of EhMGL1 activity with L-methionine
-
?
DL-homocysteine + H2O

?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O
?
-
-
-
-
?
DL-homocysteine + H2O

hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
-
?
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
?
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
?
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
-
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
?
DL-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
highest substrate specificity for DL-homocysteine
-
-
?
ethionine

?
-
-
-
?
L-cystathionine + H2O

?
assay at pH 8.0, 37°C
-
-
?
L-cystathionine + H2O
?
-
-
-
-
?
L-cystathionine + H2O
?
-
-
-
-
?
L-cysteine

?
-
-
-
?
L-cysteine + H2O

?
-
-
-
-
?
L-cysteine + H2O
?
assay at pH 8.0, 37°C
-
-
?
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O
?
-
-
-
-
-
L-cysteine + H2O
?
-
-
-
-
?
L-cysteine + H2O

sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
160% of EhMGL1 activity with L-methionine
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
20% of activity with L-methionine
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-cysteine + H2O
sulfide + NH3 + pyruvate
-
-
-
?
L-ethionine + H2O

?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O
?
-
-
-
-
-
L-ethionine + H2O
?
-
-
-
-
?
L-ethionine + H2O

ethanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-ethionine + H2O
ethanethiol + NH3 + 2-oxobutanoate
-
60% of activity with L-methinine
-
?
L-homocysteine

2-oxobutanoate + NH3 + hydrogen sulfide
-
-
methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview
-
?
L-homocysteine
2-oxobutanoate + NH3 + hydrogen sulfide
-
-
methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview
-
?
L-homocysteine + H2O

?
-
-
-
-
?
L-homocysteine + H2O
?
-
-
-
-
?
L-homocysteine + H2O

hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
-
?
L-homocysteine + H2O
hydrogen sulfide + NH3 + 2-oxobutanoate
-
-
-
-
?
L-homocysteine + H2O

sulfide + NH3 + 2-oxobutanoate
-
-
-
?
L-homocysteine + H2O
sulfide + NH3 + 2-oxobutanoate
-
-
-
?
L-homoserine + H2O

?
-
-
-
-
?
L-homoserine + H2O
?
-
-
-
-
?
L-homoserine + H2O
?
-
-
-
-
?
L-methionine

?
-
bacterial metabolism of methionine
-
-
-
L-methionine
?
-
bacterial metabolism of methionine
-
-
-
L-methionine

methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O

?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
assay at pH 8.0, 37°C
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O
?
-
-
-
-
?
L-methionine + H2O

methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
part of the methanethiol produced can react with an activated form of serine to produce S-methylcysteine. Product 2-oxobutanoate enters the papthway of Ile synthesis in plastids, part of the methanethiol produced can react with an activated form of serine to produce S-methylcysteine
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction, initial aldimine, quinonoid, and ketimine intermediates of the gamma-elimination reaction, possible concerted mechanisms of reversible ketimine formation and pro-(R)-Calpha-proton exchange, overview
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
181% of EhMGL1 activity with L-methionine
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
ir
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
ir
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
gamma-elimination reaction
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
-
?
L-methionine + H2O
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine sulfone

?
-
-
-
-
?
L-methionine sulfone
?
-
-
-
?
L-methionine sulfoxide + H2O

?
-
gamma-elimination reaction
-
-
?
L-methionine sulfoxide + H2O
?
-
gamma-elimination reaction
-
-
?
L-methionine sulfoxide + H2O
?
-
gamma-elimination reaction
-
-
?
L-methionine sulfoxide + H2O
?
-
gamma-elimination reaction
-
-
?
L-vinylglycine

?
-
-
-
-
?
O-acetyl-L-serine + H2O

2-oxopropanoate + NH3 + acetate
-
-
-
-
?
O-acetyl-L-serine + H2O
2-oxopropanoate + NH3 + acetate
-
-
-
?
O-acetyl-L-serine + H2O
2-oxopropanoate + NH3 + acetate
11% of activity with L-methionine
-
?
O-acetyl-L-serine + H2O
2-oxopropanoate + NH3 + acetate
34% of EhMGL1 activity with L-methionine
-
?
O-acetyl-L-serine + H2O
2-oxopropanoate + NH3 + acetate
-
weak activity
-
-
?
S-benzyl-L-cysteine + H2O

?
-
beta-elimination reaction
-
-
?
S-benzyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-benzyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-benzyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-benzyl-L-cysteine + H2O

thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-benzyl-L-cysteine + H2O
thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
-
-
?
S-benzyl-L-cysteine + H2O
thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-benzyl-L-cysteine + H2O
thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
-
-
?
S-ethyl-L-cysteine + H2O

?
-
beta-elimination reaction
-
-
?
S-ethyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-ethyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-ethyl-L-cysteine + H2O
?
-
beta-elimination reaction
-
-
?
S-ethyl-L-cysteine + H2O

ethanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + 2-oxopropanoate
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-ethyl-L-cysteine + H2O
ethanethiol + NH3 + 2-oxopropanoate
-
weak activity
-
-
?
S-ethyl-L-homocysteine + H2O

?
-
gamma-elimination reaction
-
-
?
S-ethyl-L-homocysteine + H2O
?
-
gamma-elimination reaction
-
-
?
S-ethyl-L-homocysteine + H2O
?
-
gamma-elimination reaction
-
-
?
S-ethyl-L-homocysteine + H2O
?
-
gamma-elimination reaction
-
-
?
S-methyl-L-cysteine + H2O

methanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + NH3 + 2-oxopropanoate
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + NH3 + 2-oxopropanoate
-
-
-
-
?
S-methyl-L-cysteine + H2O
methanethiol + NH3 + 2-oxopropanoate
-
weak activity
-
-
?
S-methyl-L-cysteine + H2O

pyruvate + NH3 + CH3SH
-
-
-
-
?
S-methyl-L-cysteine + H2O
pyruvate + NH3 + CH3SH
-
-
-
-
?
S-methyl-L-cysteine + H2O
pyruvate + NH3 + CH3SH
-
-
-
ir
Se-methylselenocysteine

?
-
-
-
-
-
Se-methylselenocysteine
?
-
-
-
?
selenomethionine + H2O

methylselenol + NH3 + 2-oxobutanoate
-
-
-
-
?
selenomethionine + H2O
methylselenol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine

?
additional information
-
bacterial metabolism of methionine
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
multicatalytic: alpha,gamma-elimination and gamma-replacement reactions of L-methionine and its analogs, alpha, beta-elimination and beta-replacement of L-cysteine and its analogs, deamination and gamma-addition of vinylglycine and deuterium labelling at alpha and beta position of L-methionine and other straight-chain L-amino acids
-
-
additional information
?
-
-
no substrate: L-cysteine
-
-
-
additional information
?
-
-
determination of rate constants of the enzyme-catalyzed exchange of Calpha and Cbeta-protons with deuterium, as well as the kinetic isotope effect of the deuterium label in the Calpha-position of inhibitors on the rate of exchange of their beta-protons. Neither stereoselectivity in the beta-proton exchange nor noticeable isotope effect on the exchange rates of beta-protons is found
-
-
-
additional information
?
-
-
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
-
-
-
additional information
?
-
-
enzyme additionally catalyzes the beta-elimination reaction of (+-)-S-alk(en)yl-L-cysteine sulfoxides to yield thiosulfinate, reaction of EC 4.4.1.4. It can decompose both diastereomers equally
-
-
-
additional information
?
-
-
enzyme catalyzes the exchange of both Calpha-protons of glycine with high stereospecificity for pro-R-proton. The reaction mechanism includes ketimine intermediate formation
-
-
-
additional information
?
-
-
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
-
-
-
additional information
?
-
-
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
-
-
-
additional information
?
-
under normal conditions, isoform ehMGL1 is involved in degradation of L-methionine
-
-
-
additional information
?
-
under normal conditions, isoform ehMGL2 is not involved degradation of L-methionine
-
-
-
additional information
?
-
-
using whole-cell lysate of Ferroplasma acidarmanus fer1, pyridoxal 5'-phosphate-dependent and L-methionine-dependent production of alpha-keto compounds and thiol groups is demonstrated, thus indicating the presence of methionine gamma-lyase in this acidophilic archaeon
-
-
-
additional information
?
-
-
using whole-cell lysate of Ferroplasma acidarmanus fer1, pyridoxal 5'-phosphate-dependent and L-methionine-dependent production of alpha-keto compounds and thiol groups is demonstrated, thus indicating the presence of methionine gamma-lyase in this acidophilic archaeon
-
-
-
additional information
?
-
additional information
-
-
-
-
additional information
?
-
-
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
-
-
-
additional information
?
-
-
beta-exchange between cysteine and alkanethiols, exchange reaction between substituents of gamma-carbon of homocysteine and alkanethiols, forming corresponding S-alkylhomocysteine
-
-
additional information
?
-
-
not: D-methionine, 2-oxo-4-methylthiobutanoate, non-sulfur-containing amino acids
-
-
additional information
?
-
-
multicatalytic: alpha,gamma-elimination and gamma-replacement reactions of L-methionine and its analogs, alpha, beta-elimination and beta-replacement of L-cysteine and its analogs, deamination and gamma-addition of vinylglycine and deuterium labelling at alpha and beta position of L-methionine and other straight-chain L-amino acids
-
-
additional information
?
-
-
no activity with O-phospho-L-serine
-
-
-
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1.25
1-amino-3-(methylthio)propylphosphinic acid
-
30°C
18.2
2-amino-3-(N,N-dimethylamino)propionic acid
-
-
12.3
DL-2-amino-3(N-hydroxyethyl)propionic acid
-
-
2.3
DL-amino-3-(N-methylamino)propionic acid
-
-
1.2
L-1-amino-3-methylthiopropylphosphinic acid
-
pH and temperature not specified in the publication
6.7
L-Gly(vinyl)
-
30°C, pH 8.0
1.78 - 6.43
L-homocysteine
4 - 38
L-methionine sulfone
6.2 - 33.51
L-methionine sulfoxide
2.3
L-selenodjenkolate
-
-
6.7 - 7.22
L-Vinylglycine
5.82
O-acetyl-DL-homoserine
-
pH 8.0, 30°C
2.22 - 6
O-acetyl-L-homoserine
2.88 - 52.33
O-acetyl-L-serine
2.8
O-methyl-L-serine
-
-
0.18 - 2.75
S-Benzyl-L-cysteine
0.17 - 8.19
S-ethyl-L-cysteine
0.278 - 8.11
S-ethyl-L-homocysteine
0.4 - 2.62
S-methyl-L-cysteine
0.77
S-methylcysteine
-
-
0.13
Se-methyl-L-selenocysteine
-
-
0.1 - 5.45
trifluoromethionine
additional information
L-methionine
0.44
alliin

-
mutant C115H, pH 8.0, 30°C
1.75
alliin
-
mutant C115A, pH 8.0, 30°C
3.6
cysteine

-
recombinant, MGL2 C116G
8.5
cysteine
-
recombinant MGL1
9.7
cysteine
-
recombinant MGL1, C113G
22.3
cysteine
-
recombinant MGL2
0.2
DL-homocysteine

-
pH 8.0, 37°C, recombinant mutant Y114F/C116H
0.24
DL-homocysteine
-
mutant enzyme C116S, at 37°C
0.6
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240R
0.8
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240N
0.97
DL-homocysteine
recombinant enzyme
0.97
DL-homocysteine
-
pH 8.0, 30°C
1
DL-homocysteine
-
30°C, pH 8.0
1.1
DL-homocysteine
wild-type
1.1
DL-homocysteine
-
pH 8.0, 37°C, recombinant wild-type enzyme
1.4
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant C166H; pH 8.0, 37°C, recombinant mutant K240M
1.47
DL-homocysteine
wild-type ehMGL2, pH 7.0, 37°C
1.61
DL-homocysteine
-
mutant enzyme C116A, at 37°C
1.8
DL-homocysteine
-
mutant enzyme C116H, at 37°C
1.82
DL-homocysteine
-
wild type enzyme, at 37°C
1.87
DL-homocysteine
36°C, pH 6.8, recombinant EhMGL2
3.03
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
3.4
DL-homocysteine
36°C, pH 6.8, recombinant EhMGL1
3.46
DL-homocysteine
-
mutant Y58F, pH 8.0, 30°C
6
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240I
56.5
DL-homoserine

-
30°C, pH 8.0
56.5
DL-homoserine
-
pH 8.0, 30°C
0.457
ethiin

-
mutant C115H, pH 8.0, 30°C
5.48
ethiin
-
mutant C115A, pH 8.0, 30°C
0.12
L-cysteine

-
mutant enzyme C116S, at 37°C
0.16
L-cysteine
recombinant enzyme
0.16
L-cysteine
-
30°C, pH 8.0
0.18
L-cysteine
wild-type
0.18
L-cysteine
pH 7.6, 37°C
0.2
L-cysteine
-
pH 8.0, 37°C, recombinant mutant Y114F/C116H; pH 8.0, 37°C, recombinant wild-type enzyme
0.2
L-cysteine
pH 8.0, 37°C
0.3
L-cysteine
-
pH 8.0, 37°C, recombinant mutant C166H; pH 8.0, 37°C, recombinant mutants K240R and K240M
0.32
L-cysteine
pH 7.6, 37°C
0.34
L-cysteine
mutant C110G, pH 7.0, 37°C
0.39
L-cysteine
-
mutant enzyme C116A, at 37°C
0.46
L-cysteine
mutant C110S, pH 7.0, 37°C
0.53
L-cysteine
-
wild type enzyme, at 37°C
0.63
L-cysteine
-
mutant enzyme C116H, at 37°C
0.63
L-cysteine
pH 7.6, 37°C
0.64
L-cysteine
wild-type ehMGL1, pH 7.0, 37°C
1
L-cysteine
-
pH 8.0, 37°C, recombinant mutants K240I and K240N
1.01
L-cysteine
mutant Y108F, pH 7.0, 37°C
1.7
L-cysteine
wild-type ehMGL2, pH 7.0, 37°C
0.2
L-ethionine

-
37°C, pH 8.0
0.27
L-ethionine
-
37°C, pH 8.0
0.54
L-ethionine
recombinant enzyme
0.56
L-ethionine
wild-type
6.2
L-ethionine
-
recombinant MGL2, C116G
12.2
L-ethionine
-
recombinant MGL1
15.2
L-ethionine
-
recombinant MGL1, C113G
37.2
L-ethionine
-
recombinant MGL2
1.78
L-homocysteine

pH 8.0, 37°C
30
L-homoserine

-
37°C, pH 8.0
0.0013
L-methionine

-
-
0.04
L-methionine
-
pH 8.0, 37°C, recombinant mutant K240R
0.11
L-methionine
-
mutant enzyme C116S, at 37°C
0.19
L-methionine
mutant C110G, pH 7.0, 37°C
0.3
L-methionine
-
mutant C115A, pH 8.0, 30°C
0.432
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
0.5
L-methionine
-
pH 8.0, 37°C, recombinant wild-type enzyme
0.61
L-methionine
wild-type ehMGL1, pH 7.0, 37°C
0.7
L-methionine
recombinant enzyme; wild-type
0.7
L-methionine
-
30°C, pH 8.0
0.7
L-methionine
-
pH 8.0, 30°C
0.72
L-methionine
mutant C110S, pH 7.0, 37°C
0.8
L-methionine
-
wild-type, pH 7.2, 30°C
0.9
L-methionine
-
37°C, pH 8.0
0.92
L-methionine
-
wild type enzyme, at 37°C
0.94
L-methionine
36°C, pH 6.8, recombinant EhMGL1
0.947
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
1.2
L-methionine
pH 7.5, 37°C
1.42
L-methionine
-
mutant enzyme C116H, at 37°C
1.6
L-methionine
-
mutant P360Q, pH 7.2, 30°C
1.6
L-methionine
pH 8.0, 37°C
1.7
L-methionine
-
37°C, pH 8.0
1.77
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
1.9
L-methionine
36°C, pH 6.8, recombinant EhMGL2
2.3
L-methionine
-
pH 8.0, 37°C, recombinant mutant C166H
2.7
L-methionine
-
mutant V358Y, pH 7.2, 30°C
2.85
L-methionine
-
mutant enzyme C116A, at 37°C
3.58
L-methionine
wild-type ehMGL2, pH 7.0, 37°C
3.7
L-methionine
-
mutant P357I, pH 7.2, 30°C
4.8
L-methionine
-
pH 8.0, 37°C, recombinant mutant K240I
5.5
L-methionine
-
mutant A366Y, pH 7.2, 30°C
5.7
L-methionine
-
pH 8.0, 37°C, recombinant mutant K240M
9.8
L-methionine
-
pH 8.0, 37°C, recombinant mutant K240N
10.6
L-methionine
-
pH 7.2, 30°C
15.12
L-methionine
mutant C113S, pH 7.0, 37°C
19.65
L-methionine
-
mutant Y58F, pH 8.0, 30°C
98
L-methionine
-
pH 8.0, 23°C
4
L-methionine sulfone

-
pH 8.0, 30°C
8.22
L-methionine sulfone
-
37°C, pH 8.0
38
L-methionine sulfone
pH 7.5, 37°C
6.2
L-methionine sulfoxide

-
pH 8.0, 30°C
7.07
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
12.22
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
33.51
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
6.7
L-Vinylglycine

-
pH 8.0, 30°C
7.22
L-Vinylglycine
-
37°C, pH 8.0
0.57
methiin

-
mutant C115H, pH 8.0, 30°C
0.63
methiin
-
mutant C115A, pH 8.0, 30°C
2.22
O-acetyl-L-homoserine

-
37°C, pH 8.0
2.46
O-acetyl-L-homoserine
-
mutant C115H, pH 8.0, 30°C
6
O-acetyl-L-homoserine
-
mutant C115A, pH 8.0, 30°C
2.88
O-acetyl-L-serine

-
mutant C115A, pH 8.0, 30°C
3.68
O-acetyl-L-serine
-
mutant C115H, pH 8.0, 30°C
6.28
O-acetyl-L-serine
wild-type ehMGL1, pH 7.0, 37°C
52.33
O-acetyl-L-serine
wild-type ehMGL2, pH 7.0, 37°C
0.18
S-Benzyl-L-cysteine

recombinant enzyme
0.18
S-Benzyl-L-cysteine
-
30°C, pH 8.0
0.19
S-Benzyl-L-cysteine
wild-type
0.348
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.766
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
1.47
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
2.75
S-Benzyl-L-cysteine
-
mutant Y58F, pH 8.0, 30°C
0.17
S-ethyl-L-cysteine

recombinant enzyme
0.17
S-ethyl-L-cysteine
-
30°C, pH 8.0
0.17
S-ethyl-L-cysteine
-
pH and temperature not specified in the publication
0.26
S-ethyl-L-cysteine
-
mutant C115A, pH 8.0, 30°C
0.358
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.48
S-ethyl-L-cysteine
-
37°C, pH 8.0
0.49
S-ethyl-L-cysteine
wild-type
0.72
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
0.93
S-ethyl-L-cysteine
-
mutant C115H, pH 8.0, 30°C
2.17
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.19
S-ethyl-L-cysteine
-
mutant Y58F, pH 8.0, 30°C
0.278
S-ethyl-L-homocysteine

-
pH 8.0, 30°C, recombinant enzyme
0.5
S-ethyl-L-homocysteine
-
30°C, pH 8.0
0.545
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
0.93
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
8.11
S-ethyl-L-homocysteine
-
mutant Y58F, pH 8.0, 30°C
0.4
S-methyl-L-cysteine

-
37°C, pH 8.0
0.4
S-methyl-L-cysteine
-
pH 8.0, 37°C, recombinant mutant Y114F/C116H
0.61
S-methyl-L-cysteine
wild-type
0.61
S-methyl-L-cysteine
-
-
0.69
S-methyl-L-cysteine
-
-
0.7
S-methyl-L-cysteine
-
pH 8.0, 37°C, recombinant wild-type enzyme
0.71
S-methyl-L-cysteine
recombinant enzyme
0.71
S-methyl-L-cysteine
-
30°C, pH 8.0
0.77
S-methyl-L-cysteine
-
-
0.8
S-methyl-L-cysteine
-
mutant C115A, pH 8.0, 30°C
1.4
S-methyl-L-cysteine
-
-
2.1
S-methyl-L-cysteine
-
pH 8.0, 37°C, recombinant mutant C166H
2.62
S-methyl-L-cysteine
-
mutant C115H, pH 8.0, 30°C
0.1
trifluoromethionine

wild-type ehMGL1, pH 7.0, 37°C
0.1
trifluoromethionine
-
-
0.29
trifluoromethionine
mutant Y111F, pH 7.0, 37°C
0.57
trifluoromethionine
mutant Y108F, pH 7.0, 37°C
0.83
trifluoromethionine
mutant R55A, pH 7.0, 37°C
0.92
trifluoromethionine
wild-type ehMGL2, pH 7.0, 37°C
1.62
trifluoromethionine
mutant R58A, pH 7.0, 37°C
5.45
trifluoromethionine
mutant C113S, pH 7.0, 37°C
additional information
L-methionine

-
values between 0.8 to 1.7, depending on publications
additional information
additional information
-
values for native and cyanylated enzyme
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics for beta- and gamma-elimination reactions, overview
-
additional information
additional information
-
steady-state kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.057 - 71
DL-homocysteine
1.9
L-Gly(vinyl)
-
30°C, pH 8.0
1.7
L-homocysteine
pH 8.0, 37°C
2.1 - 40.4
L-methionine sulfone
2.5 - 6.7
L-methionine sulfoxide
1.9 - 44.4
L-Vinylglycine
2.1
O-acetyl-DL-homoserine
-
pH 8.0, 30°C
6.23 - 78
O-acetyl-L-homoserine
1.35 - 6.22
O-acetyl-L-serine
3.34 - 10.9
S-Benzyl-L-cysteine
0.57 - 8.05
S-ethyl-L-cysteine
2.07 - 7.05
S-ethyl-L-homocysteine
0.12 - 46.3
S-methyl-L-cysteine
0.24 - 17.46
trifluoromethionine
additional information
L-methionine
0.41
alliin

-
mutant C115A, pH 8.0, 30°C
11.31
alliin
-
mutant C115H, pH 8.0, 30°C
0.057 - 0.65
DL-homocysteine

wild-type ehMGL2, pH 7.0, 37°C
0.88
DL-homocysteine
-
mutant Y58F, pH 8.0, 30°C
1.36
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.74
DL-homocysteine
-
mutant enzyme C116A, at 37°C
2.59
DL-homocysteine
-
mutant enzyme C116S, at 37°C
3.13
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
3.91
DL-homocysteine
wild-type ehMGL1, pH 7.0, 37°C
5.03
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240N
5.1
DL-homocysteine
wild-type
8.5
DL-homocysteine
-
30°C, pH 8.0
8.5
DL-homocysteine
-
pH 8.0, 30°C
8.51
DL-homocysteine
recombinant enzyme
10.56
DL-homocysteine
wild-type ehMGL2, pH 7.0, 37°C
22.7
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240R
28.8
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240M
30
DL-homocysteine
-
mutant enzyme C116H, at 37°C
44.84
DL-homocysteine
-
wild type enzyme, at 37°C
48.4
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant C166H
62.2
DL-homocysteine
-
pH 8.0, 37°C, recombinant mutant K240I
71
DL-homocysteine
-
pH 8.0, 37°C, recombinant wild-type enzyme
0.5
DL-homoserine

-
30°C, pH 8.0
0.52
DL-homoserine
-
pH 8.0, 30°C
1.25
ethiin

-
mutant C115A, pH 8.0, 30°C
5.59
ethiin
-
mutant C115H, pH 8.0, 30°C
0.03
L-cysteine

pH 8.0, 37°C
0.43
L-cysteine
-
mutant enzyme C116A, at 37°C
0.57
L-cysteine
-
mutant enzyme C116S, at 37°C
0.67
L-cysteine
mutant Y108F, pH 7.0, 37°C
0.69
L-cysteine
pH 7.6, 37°C
0.78
L-cysteine
mutant C110S, pH 7.0, 37°C
0.79
L-cysteine
pH 7.6, 37°C
0.8
L-cysteine
wild-type ehMGL2, pH 7.0, 37°C
0.89
L-cysteine
-
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.01
L-cysteine
mutant C110G, pH 7.0, 37°C
1.06
L-cysteine
-
pH 8.0, 37°C, recombinant mutant K240N
1.45
L-cysteine
-
pH 8.0, 37°C, recombinant mutant K240R
1.59
L-cysteine
wild-type ehMGL1, pH 7.0, 37°C
1.66
L-cysteine
-
pH 8.0, 37°C, recombinant mutant K240M
1.86
L-cysteine
pH 7.6, 37°C
2.13
L-cysteine
-
pH 8.0, 37°C, recombinant wild-type enzyme
2.23
L-cysteine
-
pH 8.0, 37°C, recombinant mutant K240I
2.24
L-cysteine
wild-type
2.27
L-cysteine
-
wild type enzyme, at 37°C
2.3
L-cysteine
-
30°C, pH 8.0
2.33
L-cysteine
recombinant enzyme
23.3
L-cysteine
-
pH 8.0, 37°C, recombinant mutant C166H
43.8
L-cysteine
-
mutant enzyme C116H, at 37°C
0.017
L-ethionine

-
37°C, pH 8.0
6.2
L-ethionine
wild-type
6.78
L-ethionine
recombinant enzyme
33.4
L-ethionine
-
37°C, pH 8.0
0.03
L-methionine

-
pH 8.0, 37°C, recombinant mutant C166H
0.07
L-methionine
-
mutant enzyme C116H, at 37°C
0.1
L-methionine
-
pH 8.0, 23°C
0.17
L-methionine
-
37°C, pH 8.0
0.22
L-methionine
-
mutant C115A, pH 8.0, 30°C
0.36
L-methionine
mutant C110G, pH 7.0, 37°C
0.47
L-methionine
mutant C113S, pH 7.0, 37°C
0.93
L-methionine
mutant C110S, pH 7.0, 37°C
1.11
L-methionine
wild-type ehMGL2, pH 7.0, 37°C
1.46
L-methionine
-
mutant enzyme C116A, at 37°C
1.77
L-methionine
-
mutant enzyme C116S, at 37°C
1.82
L-methionine
wild-type ehMGL1, pH 7.0, 37°C
2.66
L-methionine
-
mutant Y58F, pH 8.0, 30°C
2.9
L-methionine
pH 8.0, 37°C
3.9
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
5.47
L-methionine
pH 7.5, 37°C
6.2
L-methionine
recombinant enzyme
6.2
L-methionine
-
30°C, pH 8.0
6.2
L-methionine
-
pH 8.0, 30°C
6.5
L-methionine
wild-type
7.4
L-methionine
-
mutant P360Q, pH 7.2, 30°C
7.9
L-methionine
-
wild-type, pH 7.2, 30°C
8
L-methionine
-
mutant A366Y, pH 7.2, 30°C
9
L-methionine
-
mutant P357I, pH 7.2, 30°C
9.86
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
12
L-methionine
-
pH 8.0, 30°C, recombinant enzyme
15.1
L-methionine
-
mutant V358Y, pH 7.2, 30°C
25.39
L-methionine
-
wild type enzyme, at 37°C
33.4
L-methionine
-
pH 8.0, 37°C, recombinant wild-type enzyme
48.6
L-methionine
-
37°C, pH 8.0
2.1
L-methionine sulfone

-
pH 8.0, 30°C
34.13
L-methionine sulfone
pH 7.5, 37°C
40.4
L-methionine sulfone
-
37°C, pH 8.0
2.5
L-methionine sulfoxide

-
pH 8.0, 30°C
2.7
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
5.05
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
6.7
L-methionine sulfoxide
-
pH 8.0, 30°C, recombinant enzyme
1.9
L-Vinylglycine

-
pH 8.0, 30°C
44.4
L-Vinylglycine
-
37°C, pH 8.0
1.63
methiin

-
mutant C115A, pH 8.0, 30°C
5.14
methiin
-
mutant C115H, pH 8.0, 30°C
6.23
O-acetyl-L-homoserine

-
mutant C115A, pH 8.0, 30°C
18.29
O-acetyl-L-homoserine
-
mutant C115H, pH 8.0, 30°C
78
O-acetyl-L-homoserine
-
37°C, pH 8.0
1.35
O-acetyl-L-serine

-
mutant C115A, pH 8.0, 30°C
1.74
O-acetyl-L-serine
wild-type ehMGL1, pH 7.0, 37°C
2.93
O-acetyl-L-serine
-
mutant C115H, pH 8.0, 30°C
6.22
O-acetyl-L-serine
wild-type ehMGL2, pH 7.0, 37°C
3.34
S-Benzyl-L-cysteine

-
mutant Y58F, pH 8.0, 30°C
5.8
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.16
S-Benzyl-L-cysteine
recombinant enzyme
8.2
S-Benzyl-L-cysteine
-
30°C, pH 8.0
8.5
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
10
S-Benzyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
10.9
S-Benzyl-L-cysteine
wild-type
0.57
S-ethyl-L-cysteine

-
mutant C115A, pH 8.0, 30°C
2.38
S-ethyl-L-cysteine
-
mutant Y58F, pH 8.0, 30°C
5
S-ethyl-L-cysteine
-
30°C, pH 8.0
5.03
S-ethyl-L-cysteine
recombinant enzyme
5.79
S-ethyl-L-cysteine
-
37°C, pH 8.0
6.3
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
6.5
S-ethyl-L-cysteine
-
mutant C115H, pH 8.0, 30°C
6.8
S-ethyl-L-cysteine
wild-type
7.08
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
8.05
S-ethyl-L-cysteine
-
pH 8.0, 30°C, recombinant enzyme
2.07
S-ethyl-L-homocysteine

-
mutant Y58F, pH 8.0, 30°C
3.84
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
5.89
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
6.8
S-ethyl-L-homocysteine
-
30°C, pH 8.0
7.05
S-ethyl-L-homocysteine
-
pH 8.0, 30°C, recombinant enzyme
0.12
S-methyl-L-cysteine

-
pH 8.0, 37°C, recombinant mutant Y114F/C116H
1.38
S-methyl-L-cysteine
-
mutant C115A, pH 8.0, 30°C
1.58
S-methyl-L-cysteine
-
pH 8.0, 37°C, recombinant wild-type enzyme
4.6
S-methyl-L-cysteine
recombinant enzyme
4.6
S-methyl-L-cysteine
-
30°C, pH 8.0
5.53
S-methyl-L-cysteine
-
37°C, pH 8.0
5.9
S-methyl-L-cysteine
-
-
5.97
S-methyl-L-cysteine
wild-type
23.08
S-methyl-L-cysteine
-
mutant C115H, pH 8.0, 30°C
46.3
S-methyl-L-cysteine
-
pH 8.0, 37°C, recombinant mutant C166H
0.24
trifluoromethionine

mutant C113S, pH 7.0, 37°C
0.81
trifluoromethionine
wild-type ehMGL1, pH 7.0, 37°C
0.81
trifluoromethionine
-
-