Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.11 extracted from

  • Raboni, S.; Revtovich, S.; Demitri, N.; Giabbai, B.; Storici, P.; Cocconcelli, C.; Faggiano, S.; Rosini, E.; Pollegioni, L.; Galati, S.; Buschini, A.; Morozova, E.; Kulikova, V.; Nikulin, A.; Gabellieri, E.; Cioni, P.; Demidkina, T.; Mozzarelli, A.
    Engineering methionine gamma-lyase from Citrobacter freundii for anticancer activity (2018), Biochim. Biophys. Acta, 1866, 1260-1270 .
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
medicine mutant V358Y selectively inhibits cancer cell growth with a half-maximal inhibitory concentration almost 2fold lower than the wild type Citrobacter freundii

Cloned(Commentary)

Cloned (Comment) Organism
-
 Citrobacter freundii

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant V358Y, to 1.45 A resolution Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
A366Y mutant of the C-terminal flexible loop at the active site entry channel Citrobacter freundii
additional information site-saturation mutagenesis at residues of the C-terminal flexible loop at the active site entry channel, P357, V358, P360 and A366 Citrobacter freundii
P357I mutant of the C-terminal flexible loop at the active site entry channel Citrobacter freundii
P360Q mutant of the C-terminal flexible loop at the active site entry channel Citrobacter freundii
V358Y mutant exhibits a 1.9fold increase in the catalytic rate and a 3fold increase in Km value, catalytic efficiency is similar to wild type MGL. The cytotoxic activity towards a panel of cancer and nonmalignant cell lines is lower than that of wild-type Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 L-methionine wild-type, pH 7.2, 30°C Citrobacter freundii
1.6
-
 L-methionine mutant P360Q, pH 7.2, 30°C Citrobacter freundii
2.7
-
 L-methionine mutant V358Y, pH 7.2, 30°C Citrobacter freundii
3.7
-
 L-methionine mutant P357I, pH 7.2, 30°C Citrobacter freundii
5.5
-
 L-methionine mutant A366Y, pH 7.2, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q84AR1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.4
-
 L-methionine mutant P360Q, pH 7.2, 30°C Citrobacter freundii
7.9
-
 L-methionine wild-type, pH 7.2, 30°C Citrobacter freundii
8
-
 L-methionine mutant A366Y, pH 7.2, 30°C Citrobacter freundii
9
-
 L-methionine mutant P357I, pH 7.2, 30°C Citrobacter freundii
15.1
-
 L-methionine mutant V358Y, pH 7.2, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 L-methionine mutant A366Y, pH 7.2, 30°C Citrobacter freundii
2.4
-
 L-methionine mutant P357I, pH 7.2, 30°C Citrobacter freundii
4.6
-
 L-methionine mutant P360Q, pH 7.2, 30°C Citrobacter freundii
5.6
-
 L-methionine mutant V358Y, pH 7.2, 30°C Citrobacter freundii
9.7
-
 L-methionine wild-type, pH 7.2, 30°C Citrobacter freundii