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Literature summary for 4.4.1.11 extracted from

  • Song, H.; Xu, R.; Guo, Z.
    Identification and characterization of a methionine gamma-lyase in the calicheamicin biosynthetic cluster of Micromonospora echinospora (2015), ChemBioChem, 16, 100-109 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Micromonospora echinospora

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.0 A resolution. Active site resiudes are G105 and V322 Micromonospora echinospora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
98
-
L-methionine pH 8.0, 23°C Micromonospora echinospora

Organism

Organism UniProt Comment Textmining
Micromonospora echinospora Q8KNG3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine + H2O
-
Micromonospora echinospora methanethiol + NH3 + 2-oxobutanoate
-
?

Synonyms

Synonyms Comment Organism
CalE6
-
Micromonospora echinospora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
L-methionine pH 8.0, 23°C Micromonospora echinospora

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate protein-to-cofactor ratio is 2:1. The cofactor is removable by incubating with hydroxylamine hydrochloride and is readily restored by incubation with free PLP Micromonospora echinospora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
L-methionine pH 8.0, 23°C Micromonospora echinospora