Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Citrobacter freundii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycine | - |
Citrobacter freundii | |
L-2-aminobutanoate | - |
Citrobacter freundii | |
L-alanine | - |
Citrobacter freundii | |
L-norleucine | - |
Citrobacter freundii | |
L-norvaline | - |
Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-methionine | pH 8.0, 30°C | Citrobacter freundii | |
0.97 | - |
DL-homocysteine | pH 8.0, 30°C | Citrobacter freundii | |
4 | - |
L-methionine sulfone | pH 8.0, 30°C | Citrobacter freundii | |
5.82 | - |
O-acetyl-DL-homoserine | pH 8.0, 30°C | Citrobacter freundii | |
6.2 | - |
L-methionine sulfoxide | pH 8.0, 30°C | Citrobacter freundii | |
6.7 | - |
L-Vinylglycine | pH 8.0, 30°C | Citrobacter freundii | |
56.5 | - |
DL-homoserine | pH 8.0, 30°C | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Citrobacter freundii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-methionine + H2O = methanethiol + NH3 + 2-oxobutanoate | rate determining stage of the enzymatic reaction is deamination of aminocrotonate, important contribution of the sulfur atom and methylene groups to the efficiency of binding of substrates and inhibitors | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-homocysteine + H2O | - |
Citrobacter freundii | 2-oxobutanoate + NH3 + H2S | - |
? | |
DL-homoserine + H2O | - |
Citrobacter freundii | NH3 + 2-oxobutanoate | - |
? | |
L-methionine + H2O | - |
Citrobacter freundii | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine sulfone | - |
Citrobacter freundii | ? | - |
? | |
L-methionine sulfoxide | - |
Citrobacter freundii | ? | - |
? | |
L-vinylglycine | - |
Citrobacter freundii | ? | - |
? | |
additional information | determination of rate constants of the enzyme-catalyzed exchange of Calpha and Cbeta-protons with deuterium, as well as the kinetic isotope effect of the deuterium label in the Calpha-position of inhibitors on the rate of exchange of their beta-protons. Neither stereoselectivity in the beta-proton exchange nor noticeable isotope effect on the exchange rates of beta-protons is found | Citrobacter freundii | ? | - |
? | |
O-acetyl-DL-homoserine + H2O | - |
Citrobacter freundii | NH3 + 2-oxobutanoate + acetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
DL-homoserine | pH 8.0, 30°C | Citrobacter freundii | |
1.9 | - |
L-Vinylglycine | pH 8.0, 30°C | Citrobacter freundii | |
2.1 | - |
L-methionine sulfone | pH 8.0, 30°C | Citrobacter freundii | |
2.1 | - |
O-acetyl-DL-homoserine | pH 8.0, 30°C | Citrobacter freundii | |
2.5 | - |
L-methionine sulfoxide | pH 8.0, 30°C | Citrobacter freundii | |
6.2 | - |
L-methionine | pH 8.0, 30°C | Citrobacter freundii | |
8.5 | - |
DL-homocysteine | pH 8.0, 30°C | Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Citrobacter freundii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
L-norleucine | pH 8.0, 30°C | Citrobacter freundii | |
3.4 | - |
L-alanine | pH 8.0, 30°C | Citrobacter freundii | |
4.7 | - |
L-norvaline | pH 8.0, 30°C | Citrobacter freundii | |
8.3 | - |
L-2-aminobutanoate | pH 8.0, 30°C | Citrobacter freundii | |
49 | - |
glycine | pH 8.0, 30°C | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
DL-homoserine | pH 8.0, 30°C | Citrobacter freundii | |
0.28 | - |
L-Vinylglycine | pH 8.0, 30°C | Citrobacter freundii | |
0.36 | - |
O-acetyl-DL-homoserine | pH 8.0, 30°C | Citrobacter freundii | |
0.4 | - |
L-methionine sulfoxide | pH 8.0, 30°C | Citrobacter freundii | |
0.52 | - |
L-methionine sulfone | pH 8.0, 30°C | Citrobacter freundii | |
8.8 | - |
DL-homocysteine | pH 8.0, 30°C | Citrobacter freundii | |
8.9 | - |
L-methionine | pH 8.0, 30°C | Citrobacter freundii |