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Literature summary for 4.4.1.11 extracted from

  • Morozova, E.A.; Bazhulina, N.P.; Anufrieva, N.V.; Mamaeva, D.V.; Tkachev, Y.V.; Streltsov, S.A.; Timofeev, V.P.; Faleev, N.G.; Demidkina, T.V.
    Kinetic and spectral parameters of interaction of Citrobacter freundii methionine gamma-lyase with amino acids (2010), Biochemistry (Moscow), 75, 1272-1280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

Inhibitors

Inhibitors Comment Organism Structure
glycine
-
Citrobacter freundii
L-2-aminobutanoate
-
Citrobacter freundii
L-alanine
-
Citrobacter freundii
L-norleucine
-
Citrobacter freundii
L-norvaline
-
Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
L-methionine pH 8.0, 30°C Citrobacter freundii
0.97
-
DL-homocysteine pH 8.0, 30°C Citrobacter freundii
4
-
L-methionine sulfone pH 8.0, 30°C Citrobacter freundii
5.82
-
O-acetyl-DL-homoserine pH 8.0, 30°C Citrobacter freundii
6.2
-
L-methionine sulfoxide pH 8.0, 30°C Citrobacter freundii
6.7
-
L-Vinylglycine pH 8.0, 30°C Citrobacter freundii
56.5
-
DL-homoserine pH 8.0, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Citrobacter freundii

Reaction

Reaction Comment Organism Reaction ID
L-methionine + H2O = methanethiol + NH3 + 2-oxobutanoate rate determining stage of the enzymatic reaction is deamination of aminocrotonate, important contribution of the sulfur atom and methylene groups to the efficiency of binding of substrates and inhibitors Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O
-
Citrobacter freundii 2-oxobutanoate + NH3 + H2S
-
?
DL-homoserine + H2O
-
Citrobacter freundii NH3 + 2-oxobutanoate
-
?
L-methionine + H2O
-
Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
?
L-methionine sulfone
-
Citrobacter freundii ?
-
?
L-methionine sulfoxide
-
Citrobacter freundii ?
-
?
L-vinylglycine
-
Citrobacter freundii ?
-
?
additional information determination of rate constants of the enzyme-catalyzed exchange of Calpha and Cbeta-protons with deuterium, as well as the kinetic isotope effect of the deuterium label in the Calpha-position of inhibitors on the rate of exchange of their beta-protons. Neither stereoselectivity in the beta-proton exchange nor noticeable isotope effect on the exchange rates of beta-protons is found Citrobacter freundii ?
-
?
O-acetyl-DL-homoserine + H2O
-
Citrobacter freundii NH3 + 2-oxobutanoate + acetate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
DL-homoserine pH 8.0, 30°C Citrobacter freundii
1.9
-
L-Vinylglycine pH 8.0, 30°C Citrobacter freundii
2.1
-
L-methionine sulfone pH 8.0, 30°C Citrobacter freundii
2.1
-
O-acetyl-DL-homoserine pH 8.0, 30°C Citrobacter freundii
2.5
-
L-methionine sulfoxide pH 8.0, 30°C Citrobacter freundii
6.2
-
L-methionine pH 8.0, 30°C Citrobacter freundii
8.5
-
DL-homocysteine pH 8.0, 30°C Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Citrobacter freundii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
L-norleucine pH 8.0, 30°C Citrobacter freundii
3.4
-
L-alanine pH 8.0, 30°C Citrobacter freundii
4.7
-
L-norvaline pH 8.0, 30°C Citrobacter freundii
8.3
-
L-2-aminobutanoate pH 8.0, 30°C Citrobacter freundii
49
-
glycine pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
DL-homoserine pH 8.0, 30°C Citrobacter freundii
0.28
-
L-Vinylglycine pH 8.0, 30°C Citrobacter freundii
0.36
-
O-acetyl-DL-homoserine pH 8.0, 30°C Citrobacter freundii
0.4
-
L-methionine sulfoxide pH 8.0, 30°C Citrobacter freundii
0.52
-
L-methionine sulfone pH 8.0, 30°C Citrobacter freundii
8.8
-
DL-homocysteine pH 8.0, 30°C Citrobacter freundii
8.9
-
L-methionine pH 8.0, 30°C Citrobacter freundii