Application | Comment | Organism |
---|---|---|
analysis | assay for methionine gamma-lyase-catalyzed gamma-elimination reactions of L-methionine and its analogues with 2-oxobutanoate as product. The assay employs UV-Vis spectrophotometry to continuously monitor the rate of formation of 2-oxobutanoate by its absorbance at 315 nm | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-methionine | pH 7.5, 37°C | Porphyromonas gingivalis | |
38 | - |
L-methionine sulfone | pH 7.5, 37°C | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | C3VMV9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | - |
Porphyromonas gingivalis | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-methionine sulfone + H2O | - |
Porphyromonas gingivalis | ? + NH3 + 2-oxobutanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.47 | - |
L-methionine | pH 7.5, 37°C | Porphyromonas gingivalis | |
34.13 | - |
L-methionine sulfone | pH 7.5, 37°C | Porphyromonas gingivalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-methionine sulfone | pH 7.5, 37°C | Porphyromonas gingivalis | |
4.55 | - |
L-methionine | pH 7.5, 37°C | Porphyromonas gingivalis |