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Literature summary for 4.4.1.11 extracted from
- Purification and characterization of methioninase from Pseudomonas putida (1976), J. Biochem., 79, 1263-1272.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Pseudomonas putida |  |
| hydroxylamine | - |
Pseudomonas putida | |
| iodoacetate | slightly | Pseudomonas putida | |
| N-ethylmaleimide | - |
Pseudomonas putida | |
| p-chloromercuribenzoate | - |
Pseudomonas putida | |
| Semicarbazide | - |
Pseudomonas putida | |
| sulfhydryl inhibitors | - |
Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudomonas putida | |
| 0.31 | - |
L-ethionine | - |
Pseudomonas putida | |
| 0.69 | - |
S-methyl-L-cysteine | - |
Pseudomonas putida | |
| 1.58 | - |
L-methionine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, 50-60% loss of activity within 7 days | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethionine | - |
Pseudomonas putida | ? | - |
? | |
| additional information | not: D-methionine, 2-oxo-4-methylthiobutanoate, non-sulfur-containing amino acids | Pseudomonas putida | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
several days without loss of activity | Pseudomonas putida |
| 75 | - |
30% loss of activity after 25 min | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
99% loss of activity after 24 h | Pseudomonas putida |
| 6 | 7.5 | stable for 24 h at 4°C | Pseudomonas putida |
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