Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.11 extracted from

  • Manukhov, I.V.; Mamaeva, D.V.; Morozova, E.A.; Rastorguev, S.M.; Faleev, N.G.; Demidkina, T.V.; Zavilgelsky, G.B.
    L-methionine gamma-lyase from Citrobacter freundii: cloning of the gene and kinetic parameters of the enzyme (2006), Biochemistry (Moscow), 71, 361-369.
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
synthesis presence of a hybrid plasmid in Escherichia coli K12 containing the enzyme gene leads to a decrease in efficiciency of EcoKI restriction Citrobacter freundii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 L-cysteine 30°C, pH 8.0 Citrobacter freundii
0.17
-
 S-ethyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
0.18
-
 S-Benzyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
0.5
-
 S-ethyl-L-homocysteine 30°C, pH 8.0 Citrobacter freundii
0.7
-
 L-methionine 30°C, pH 8.0 Citrobacter freundii
0.71
-
 S-methyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
1
-
 DL-homocysteine 30°C, pH 8.0 Citrobacter freundii
6.7
-
 L-Gly(vinyl) 30°C, pH 8.0 Citrobacter freundii
56.5
-
 DL-homoserine 30°C, pH 8.0 Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O
-
 Citrobacter freundii hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
DL-homoserine + H2O
-
 Citrobacter freundii NH3 + 2-oxobutanoate
-
 ?
L-cysteine
-
 Citrobacter freundii 2-oxopropanoate + NH3 + H2S
-
 ?
L-Gly(vinyl) + H2O
-
 Citrobacter freundii ?
-
 ?
L-methionine
-
 Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
S-benzyl-L-cysteine + H2O
-
 Citrobacter freundii thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
 ?
S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
S-ethyl-L-homocysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxobutanoate
-
 ?
S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 DL-homoserine 30°C, pH 8.0 Citrobacter freundii
1.9
-
 L-Gly(vinyl) 30°C, pH 8.0 Citrobacter freundii
2.3
-
 L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.6
-
 S-methyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
5
-
 S-ethyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
6.2
-
 L-methionine 30°C, pH 8.0 Citrobacter freundii
6.8
-
 S-ethyl-L-homocysteine 30°C, pH 8.0 Citrobacter freundii
8.2
-
 S-Benzyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
8.5
-
 DL-homocysteine 30°C, pH 8.0 Citrobacter freundii