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Literature summary for 4.4.1.11 extracted from

  • Hanniffy, S.B.; Philo, M.; Pelaez, C.; Gasson, M.J.; Requena, T.; Martinez-Cuesta, M.C.
    Heterologous production of methionine-gamma-lyase from Brevibacterium linens in Lactococcus lactis and formation of volatile sulfur compounds (2009), Appl. Environ. Microbiol., 75, 2326-2332.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.11

Application

Application Comment Organism
nutrition importance in flavor formation during cheese ripening Brevibacterium linens BL2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Lactococcus lactis Brevibacterium linens BL2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 SDS-PAGE Brevibacterium linens BL2

Organism

Organism UniProt Comment Textmining
Brevibacterium linens BL2 Q5MQ31
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on nickel-nitrilotriacetic acid resin Brevibacterium linens BL2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant enzyme, substrate L-cystathione, with nisin induction, value from 15 to 65 micromol/min/mg Brevibacterium linens BL2
additional information
-
 recombinant enzyme, substrate L-cystine, with nisin induction, value from 21 to 137 micromol/min/mg Brevibacterium linens BL2
additional information
-
 recombinant enzyme, substrate L-methionine, with nisin induction, value from 21 to 103 micromol/min/mg Brevibacterium linens BL2
14
-
 recombinant enzyme, substrate L-cystathionine, without nisin induction Brevibacterium linens BL2
19
-
 recombinant enzyme, substrate L-cystine or L-methionine, without nisin induction Brevibacterium linens BL2
60
-
 wild-type enzyme, substrate L-cystathionine, without nisin induction Brevibacterium linens BL2
90
-
 wild-type enzyme, substrate L-methionine, without nisin induction Brevibacterium linens BL2
123
-
 wild-type enzyme, substrate L-cystine, without nisin induction Brevibacterium linens BL2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
L-cysteine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
L-cystine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
L-methionine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?

Synonyms

Synonyms Comment Organism
methionine-gamma-lyase
-
 Brevibacterium linens BL2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Brevibacterium linens BL2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Brevibacterium linens BL2