BRENDA - Enzyme Database
show all sequences of 4.4.1.11

Microbial L-methioninase: production, molecular characterization, and therapeutic applications

El-Sayed, A.S.; Appl. Microbiol. Biotechnol. 86, 445-467 (2010)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
enzyme therapy against various types of methionine-dependent tumors
Achromobacter starkeyi
medicine
enzyme therapy against various types of methionine-dependent tumors
Aeromonas sp.
medicine
enzyme therapy against various types of methionine-dependent tumors
Aspergillus flavipes
medicine
enzyme therapy against various types of methionine-dependent tumors
Aspergillus sp.
medicine
enzyme therapy against various types of methionine-dependent tumors
Brevibacterium linens BL2
medicine
enzyme therapy against various types of methionine-dependent tumors
Citrobacter freundii
medicine
enzyme therapy against various types of methionine-dependent tumors
Citrobacter intermedius
medicine
enzyme therapy against various types of methionine-dependent tumors
Cladosporium cladosporioides
medicine
enzyme therapy against various types of methionine-dependent tumors
Clostridium sporogenes
medicine
enzyme therapy against various types of methionine-dependent tumors
Entamoeba histolytica
medicine
enzyme therapy against various types of methionine-dependent tumors
Lactococcus lactis
medicine
enyzme therapy against various types of methionine-dependent tumors; enzyme therapy against various types of methionine-dependent tumors
Pseudomonas putida
medicine
enzyme therapy against various types of methionine-dependent tumors
Treponema denticola
medicine
enzyme therapy against various types of methionine-dependent tumors
Trichomonas vaginalis
Crystallization (Commentary)
Crystallization
Organism
hanging-drop vapor-diffusion method
Aeromonas sp.
hanging-drop vapor-diffusion method
Aspergillus flavipes
hanging-drop vapor-diffusion method
Aspergillus sp.
hanging-drop vapor-diffusion method
Brevibacterium linens BL2
hanging-drop vapor-diffusion method
Citrobacter freundii
hanging-drop vapor-diffusion method
Citrobacter intermedius
hanging-drop vapor-diffusion method
Cladosporium cladosporioides
hanging-drop vapor-diffusion method
Clostridium sporogenes
hanging-drop vapor-diffusion method
Entamoeba histolytica
hanging-drop vapor-diffusion method
Lactococcus lactis
hanging-drop vapor-diffusion method
Pseudomonas putida
hanging-drop vapor-diffusion method
Treponema denticola
hanging-drop vapor-diffusion method
Trichomonas vaginalis
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Lactococcus lactis
2-mercaptoethanol
-
Aspergillus flavipes
3-Methyl-2-benzothiazolinone hydrazone
-
Lactococcus lactis
5-5'-dithiobis-(2-nitrobenzoic acid)
-
Brevibacterium linens BL2
5-5'-dithiobis-(2-nitrobenzoic acid)
-
Trichomonas vaginalis
aminoethyloxyvinylglycine
-
Achromobacter starkeyi
aminoethyloxyvinylglycine
-
Aeromonas sp.
aminoethyloxyvinylglycine
-
Aspergillus flavipes
aminoethyloxyvinylglycine
-
Aspergillus sp.
aminoethyloxyvinylglycine
-
Brevibacterium linens BL2
aminoethyloxyvinylglycine
-
Citrobacter freundii
aminoethyloxyvinylglycine
-
Citrobacter intermedius
aminoethyloxyvinylglycine
-
Cladosporium cladosporioides
aminoethyloxyvinylglycine
-
Clostridium sporogenes
aminoethyloxyvinylglycine
-
Entamoeba histolytica
aminoethyloxyvinylglycine
-
Lactococcus lactis
aminoethyloxyvinylglycine
-
Pseudomonas putida
aminoethyloxyvinylglycine
-
Treponema denticola
aminoethyloxyvinylglycine
-
Trichomonas vaginalis
DL-Penicillamine
-
Brevibacterium linens BL2
DL-Penicillamine
-
Trichomonas vaginalis
DL-propargylglycine
-
Brevibacterium linens BL2
DL-propargylglycine
-
Trichomonas vaginalis
DMSO
-
Lactococcus lactis
DMSO
-
Aspergillus flavipes
EDTA
-
Aspergillus flavipes
hydroxylamine
-
Brevibacterium linens BL2
hydroxylamine
-
Lactococcus lactis
hydroxylamine
-
Trichomonas vaginalis
iodoacetamide
-
Brevibacterium linens BL2
iodoacetamide
-
Trichomonas vaginalis
iodoacetate
-
Achromobacter starkeyi
iodoacetic acid
-
Aspergillus flavipes
iodoacetic acid
-
Lactococcus lactis
L-Cycloserine
-
Brevibacterium linens BL2
L-Cycloserine
-
Trichomonas vaginalis
p-chloromercuri-benzoic acid
-
Achromobacter starkeyi
p-chloromercuri-benzoic acid
-
Trichomonas vaginalis
p-chloromercuribenzoic acid
-
Brevibacterium linens BL2
propargylglycine
-
Achromobacter starkeyi
propargylglycine
-
Aeromonas sp.
propargylglycine
-
Aspergillus flavipes
propargylglycine
-
Aspergillus sp.
propargylglycine
-
Brevibacterium linens BL2
propargylglycine
-
Citrobacter freundii
propargylglycine
-
Citrobacter intermedius
propargylglycine
-
Cladosporium cladosporioides
propargylglycine
-
Clostridium sporogenes
propargylglycine
-
Entamoeba histolytica
propargylglycine
-
Lactococcus lactis
propargylglycine
-
Pseudomonas putida
propargylglycine
-
Treponema denticola
propargylglycine
-
Trichomonas vaginalis
Semicarbazide
-
Achromobacter starkeyi
Sodium arsenate
-
Achromobacter starkeyi
Sodium azide
-
Aspergillus flavipes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
L-methionine
values between 0.8 to 1.7, depending on publications
Pseudomonas putida
0.0013
-
L-methionine
-
Aspergillus sp.
0.1
-
trifluoromethionine
-
Entamoeba histolytica
0.18
-
L-cysteine
-
Citrobacter freundii
0.2
-
L-ethionine
-
Pseudomonas putida
0.27
-
L-cystathionine
-
Lactococcus lactis
0.45
-
L-ethionine
-
Pseudomonas putida
0.46
-
L-cysteine
-
Lactococcus lactis
0.5
-
DL-homocysteine
-
Trichomonas vaginalis
0.5
-
L-ethionine
-
Trichomonas vaginalis
0.53
-
L-cysteine
-
Pseudomonas putida
0.55
-
L-methionine
-
Treponema denticola
0.56
-
L-ethionine
-
Citrobacter freundii
0.61
-
L-methionine
-
Entamoeba histolytica
0.61
-
S-methyl-L-cysteine
-
Citrobacter freundii
0.64
-
L-cysteine
-
Entamoeba histolytica
0.7
-
L-methionine
-
Citrobacter freundii
0.77
-
S-methylcysteine
-
Pseudomonas putida
0.87
-
L-methionine
-
Lactococcus lactis
1.1
-
DL-homocysteine
-
Citrobacter freundii
1.13
-
L-methionine
-
Citrobacter intermedius
1.33
-
L-methionine
-
Pseudomonas putida
1.8
-
DL-homocysteine
-
Pseudomonas putida
3.03
-
DL-homocysteine
-
Entamoeba histolytica
3.6
-
L-cysteine
-
Trichomonas vaginalis
4.3
-
L-methionine
-
Trichomonas vaginalis
6.43
-
L-homocysteine
-
Pseudomonas putida
30
-
L-homoserine
-
Pseudomonas putida
90
-
L-methionine
-
Clostridium sporogenes
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Achromobacter starkeyi
5622
-
intracellular
-
Aeromonas sp.
5622
-
intracellular
-
Aspergillus flavipes
5622
-
intracellular
-
Aspergillus sp.
5622
-
intracellular
-
Brevibacterium linens BL2
5622
-
intracellular
-
Citrobacter freundii
5622
-
intracellular
-
Citrobacter intermedius
5622
-
intracellular
-
Cladosporium cladosporioides
5622
-
intracellular
-
Clostridium sporogenes
5622
-
intracellular
-
Entamoeba histolytica
5622
-
intracellular
-
Lactococcus lactis
5622
-
intracellular
-
Pseudomonas putida
5622
-
intracellular
-
Treponema denticola
5622
-
intracellular
-
Trichomonas vaginalis
5622
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
significant enzyme inhibition
Aspergillus flavipes
Co2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Cu2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Li+
significant enzyme inhibition
Aspergillus flavipes
Mg2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Mn2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Na+
significant enzyme inhibition
Aspergillus flavipes
Ni2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
4 * 37500, SDS-PAGE
Clostridium sporogenes
41000
-
4 * 41000, SDS-PAGE
Aeromonas sp.
42600
-
4 * 42600, SDS-PAGE
Pseudomonas putida
43000
-
4 * 43000, SDS-PAGE
Brevibacterium linens BL2
43000
-
4 * 43000, SDS-PAGE
Citrobacter freundii
43000
-
4 * 43000, SDS-PAGE
Entamoeba histolytica
44000
-
4 * 44000, SDS-PAGE
Trichomonas vaginalis
45000
-
4 * 45000, SDS-PAGE
Pseudomonas putida
55000
-
4 * 55000, SDS-PAGE
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Achromobacter starkeyi
-
-
-
Aeromonas sp.
-
-
-
Aspergillus flavipes
-
-
-
Aspergillus sp.
-
-
-
Aspergillus sp. Rs-1a
-
-
-
Brevibacterium linens BL2
-
-
-
Citrobacter freundii
-
-
-
Citrobacter intermedius
-
-
-
Cladosporium cladosporioides
-
-
-
Clostridium sporogenes
-
-
-
Entamoeba histolytica
-
-
-
Lactococcus lactis
-
-
-
no activity in mammalia
-
-
-
Pseudomonas putida
-
-
-
Treponema denticola
-
-
-
Trichomonas vaginalis
-
-
-
Purification (Commentary)
Commentary
Organism
ion exchange and gel filtration chromatography
Aeromonas sp.
ion exchange and gel filtration chromatography
Aspergillus flavipes
ion exchange and gel filtration chromatography
Aspergillus sp.
ion exchange and gel filtration chromatography
Brevibacterium linens BL2
ion exchange and gel filtration chromatography
Citrobacter freundii
ion exchange and gel filtration chromatography
Citrobacter intermedius
ion exchange and gel filtration chromatography
Cladosporium cladosporioides
ion exchange and gel filtration chromatography
Clostridium sporogenes
ion exchange and gel filtration chromatography
Entamoeba histolytica
ion exchange and gel filtration chromatography
Lactococcus lactis
ion exchange and gel filtration chromatography
Pseudomonas putida
ion exchange and gel filtration chromatography
Treponema denticola
ion exchange and gel filtration chromatography
Trichomonas vaginalis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
value is 31.0 U/mg protein
Aeromonas sp.
additional information
-
value is 61 U/mg protein
Aspergillus flavipes
additional information
-
value is 44.8 U/mg protein
Aspergillus sp.
additional information
-
value is 228.9 U/mg protein
Brevibacterium linens BL2
additional information
-
value is 10 U/mg protein
Citrobacter freundii
additional information
-
value is 0.26 U/mg protein
Citrobacter intermedius
additional information
-
value is 0.44 U/mg protein
Entamoeba histolytica
additional information
-
value is 0.04 U/mg protein
Lactococcus lactis
additional information
-
value is 1.8 U/mg protein; value is 45.3 U/mg protein
Pseudomonas putida
additional information
-
value is 36.6 U/mg protein
Treponema denticola
additional information
-
value is 3.17 U/mg protein
Trichomonas vaginalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cystathionine + ?
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-ethionine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-homocysteic acid + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-cystathionine + ?
-
701880
Lactococcus lactis
?
-
-
-
?
L-cysteine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
L-cysteine + H2O
-
701880
Lactococcus lactis
?
-
-
-
?
L-cysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-cysteine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-cysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-cysteine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-cysteine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
L-ethionine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-ethionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-ethionine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-ethionine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-ethionine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-ethionine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-ethionine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-homocysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-homocysteine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-homoserine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-homoserine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-homoserine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-methionine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
L-methionine + H2O
-
701880
Lactococcus lactis
?
-
-
-
?
L-methionine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-methionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-methionine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-methionine + H2O
-
701880
Citrobacter intermedius
?
-
-
-
?
L-methionine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-methionine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-methionine + H2O
-
701880
Treponema denticola
?
-
-
-
?
L-methionine + H2O
-
701880
Cladosporium cladosporioides
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus flavipes
?
-
-
-
?
L-methionine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-selenomethionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
O-acetyl-L-homoserine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
S-methyl-L-cysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
S-methylcysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
trifluoromethionine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
-
Achromobacter starkeyi
tetramer
4 * 41000, SDS-PAGE
Aeromonas sp.
tetramer
-
Aspergillus flavipes
tetramer
-
Aspergillus sp.
tetramer
4 * 43000, SDS-PAGE
Brevibacterium linens BL2
tetramer
4 * 43000, SDS-PAGE
Citrobacter freundii
tetramer
-
Citrobacter intermedius
tetramer
-
Cladosporium cladosporioides
tetramer
4 * 37500, SDS-PAGE
Clostridium sporogenes
tetramer
4 * 43000, SDS-PAGE
Entamoeba histolytica
tetramer
4 * 55000, SDS-PAGE
Lactococcus lactis
tetramer
4 * 42600, SDS-PAGE; 4 * 45000, SDS-PAGE
Pseudomonas putida
tetramer
4*43500, SDS-PAGE
Treponema denticola
tetramer
4 * 44000, SDS-PAGE
Trichomonas vaginalis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Brevibacterium linens BL2
30
-
-
Aspergillus flavipes
30
-
-
Citrobacter freundii
30
-
-
Citrobacter intermedius
30
-
-
Cladosporium cladosporioides
30
-
-
Lactococcus lactis
35
-
-
Aspergillus sp.
37
-
-
Entamoeba histolytica
37
-
-
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
L-methionine
values between 25.3 to 48.6, depending on publications
Pseudomonas putida
0.81
-
trifluoromethionine
-
Entamoeba histolytica
1.4
-
L-homoserine
-
Pseudomonas putida
1.59
-
L-cysteine
-
Entamoeba histolytica
1.82
-
L-methionine
-
Entamoeba histolytica
2.27
-
L-cysteine
-
Pseudomonas putida
2.3
-
L-cysteine
-
Citrobacter freundii
3.92
-
DL-homocysteine
-
Entamoeba histolytica
5.1
-
DL-homocysteine
-
Citrobacter freundii
5.9
-
S-methyl-L-cysteine
-
Citrobacter freundii
6.2
-
L-ethionine
-
Citrobacter freundii
6.5
-
L-methionine
-
Citrobacter freundii
33.4
-
L-ethionine
-
Pseudomonas putida
44.8
-
DL-homocysteine
-
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Entamoeba histolytica
7.2
-
-
Pseudomonas putida
7.5
-
-
Brevibacterium linens BL2
8
-
-
Aeromonas sp.
8
-
-
Aspergillus flavipes
8
-
-
Aspergillus sp.
8
-
-
Citrobacter freundii
8
-
-
Pseudomonas putida
9
-
-
Cladosporium cladosporioides
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8
-
Trichomonas vaginalis
7.5
8.5
-
Clostridium sporogenes
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.6
8.2
-
Entamoeba histolytica
6
10
-
Pseudomonas putida
6
8
-
Brevibacterium linens BL2
6
9
-
Aeromonas sp.
6
9
-
Pseudomonas putida
6.5
9
-
Trichomonas vaginalis
8
9
-
Aspergillus flavipes
8
9
-
Aspergillus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Achromobacter starkeyi
pyridoxal 5'-phosphate
-
Aeromonas sp.
pyridoxal 5'-phosphate
-
Aspergillus flavipes
pyridoxal 5'-phosphate
-
Aspergillus sp.
pyridoxal 5'-phosphate
-
Brevibacterium linens BL2
pyridoxal 5'-phosphate
-
Citrobacter intermedius
pyridoxal 5'-phosphate
-
Cladosporium cladosporioides
pyridoxal 5'-phosphate
-
Entamoeba histolytica
pyridoxal 5'-phosphate
-
Lactococcus lactis
pyridoxal 5'-phosphate
-
Treponema denticola
pyridoxal 5'-phosphate
-
Trichomonas vaginalis
pyridoxal 5'-phosphate
-
Clostridium sporogenes
pyridoxal 5'-phosphate
-
Citrobacter freundii
pyridoxal 5'-phosphate
-
Pseudomonas putida
Application (protein specific)
Application
Commentary
Organism
medicine
enzyme therapy against various types of methionine-dependent tumors
Achromobacter starkeyi
medicine
enzyme therapy against various types of methionine-dependent tumors
Aeromonas sp.
medicine
enzyme therapy against various types of methionine-dependent tumors
Aspergillus flavipes
medicine
enzyme therapy against various types of methionine-dependent tumors
Aspergillus sp.
medicine
enzyme therapy against various types of methionine-dependent tumors
Brevibacterium linens BL2
medicine
enzyme therapy against various types of methionine-dependent tumors
Citrobacter freundii
medicine
enzyme therapy against various types of methionine-dependent tumors
Citrobacter intermedius
medicine
enzyme therapy against various types of methionine-dependent tumors
Cladosporium cladosporioides
medicine
enzyme therapy against various types of methionine-dependent tumors
Clostridium sporogenes
medicine
enzyme therapy against various types of methionine-dependent tumors
Entamoeba histolytica
medicine
enzyme therapy against various types of methionine-dependent tumors
Lactococcus lactis
medicine
enyzme therapy against various types of methionine-dependent tumors; enzyme therapy against various types of methionine-dependent tumors
Pseudomonas putida
medicine
enzyme therapy against various types of methionine-dependent tumors
Treponema denticola
medicine
enzyme therapy against various types of methionine-dependent tumors
Trichomonas vaginalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Achromobacter starkeyi
pyridoxal 5'-phosphate
-
Aeromonas sp.
pyridoxal 5'-phosphate
-
Aspergillus flavipes
pyridoxal 5'-phosphate
-
Aspergillus sp.
pyridoxal 5'-phosphate
-
Brevibacterium linens BL2
pyridoxal 5'-phosphate
-
Citrobacter intermedius
pyridoxal 5'-phosphate
-
Cladosporium cladosporioides
pyridoxal 5'-phosphate
-
Entamoeba histolytica
pyridoxal 5'-phosphate
-
Lactococcus lactis
pyridoxal 5'-phosphate
-
Pseudomonas putida
pyridoxal 5'-phosphate
-
Treponema denticola
pyridoxal 5'-phosphate
-
Trichomonas vaginalis
pyridoxal 5'-phosphate
-
Citrobacter freundii
pyridoxal 5'-phosphate
-
Clostridium sporogenes
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging-drop vapor-diffusion method
Aeromonas sp.
hanging-drop vapor-diffusion method
Aspergillus flavipes
hanging-drop vapor-diffusion method
Aspergillus sp.
hanging-drop vapor-diffusion method
Brevibacterium linens BL2
hanging-drop vapor-diffusion method
Citrobacter freundii
hanging-drop vapor-diffusion method
Citrobacter intermedius
hanging-drop vapor-diffusion method
Cladosporium cladosporioides
hanging-drop vapor-diffusion method
Clostridium sporogenes
hanging-drop vapor-diffusion method
Entamoeba histolytica
hanging-drop vapor-diffusion method
Lactococcus lactis
hanging-drop vapor-diffusion method
Pseudomonas putida
hanging-drop vapor-diffusion method
Treponema denticola
hanging-drop vapor-diffusion method
Trichomonas vaginalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Lactococcus lactis
2-mercaptoethanol
-
Aspergillus flavipes
3-Methyl-2-benzothiazolinone hydrazone
-
Lactococcus lactis
5-5'-dithiobis-(2-nitrobenzoic acid)
-
Brevibacterium linens BL2
5-5'-dithiobis-(2-nitrobenzoic acid)
-
Trichomonas vaginalis
aminoethyloxyvinylglycine
-
Achromobacter starkeyi
aminoethyloxyvinylglycine
-
Aeromonas sp.
aminoethyloxyvinylglycine
-
Aspergillus flavipes
aminoethyloxyvinylglycine
-
Aspergillus sp.
aminoethyloxyvinylglycine
-
Brevibacterium linens BL2
aminoethyloxyvinylglycine
-
Citrobacter freundii
aminoethyloxyvinylglycine
-
Citrobacter intermedius
aminoethyloxyvinylglycine
-
Cladosporium cladosporioides
aminoethyloxyvinylglycine
-
Clostridium sporogenes
aminoethyloxyvinylglycine
-
Entamoeba histolytica
aminoethyloxyvinylglycine
-
Lactococcus lactis
aminoethyloxyvinylglycine
-
Pseudomonas putida
aminoethyloxyvinylglycine
-
Treponema denticola
aminoethyloxyvinylglycine
-
Trichomonas vaginalis
DL-Penicillamine
-
Brevibacterium linens BL2
DL-Penicillamine
-
Trichomonas vaginalis
DL-propargylglycine
-
Brevibacterium linens BL2
DL-propargylglycine
-
Trichomonas vaginalis
DMSO
-
Lactococcus lactis
DMSO
-
Aspergillus flavipes
EDTA
-
Aspergillus flavipes
hydroxylamine
-
Brevibacterium linens BL2
hydroxylamine
-
Lactococcus lactis
hydroxylamine
-
Trichomonas vaginalis
iodoacetamide
-
Brevibacterium linens BL2
iodoacetamide
-
Trichomonas vaginalis
iodoacetate
-
Achromobacter starkeyi
iodoacetic acid
-
Aspergillus flavipes
iodoacetic acid
-
Lactococcus lactis
L-Cycloserine
-
Brevibacterium linens BL2
L-Cycloserine
-
Trichomonas vaginalis
p-chloromercuri-benzoic acid
-
Achromobacter starkeyi
p-chloromercuri-benzoic acid
-
Trichomonas vaginalis
p-chloromercuribenzoic acid
-
Brevibacterium linens BL2
propargylglycine
-
Achromobacter starkeyi
propargylglycine
-
Aeromonas sp.
propargylglycine
-
Aspergillus flavipes
propargylglycine
-
Aspergillus sp.
propargylglycine
-
Brevibacterium linens BL2
propargylglycine
-
Citrobacter freundii
propargylglycine
-
Citrobacter intermedius
propargylglycine
-
Cladosporium cladosporioides
propargylglycine
-
Clostridium sporogenes
propargylglycine
-
Entamoeba histolytica
propargylglycine
-
Lactococcus lactis
propargylglycine
-
Pseudomonas putida
propargylglycine
-
Treponema denticola
propargylglycine
-
Trichomonas vaginalis
Semicarbazide
-
Achromobacter starkeyi
Sodium arsenate
-
Achromobacter starkeyi
Sodium azide
-
Aspergillus flavipes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
L-methionine
values between 0.8 to 1.7, depending on publications
Pseudomonas putida
0.0013
-
L-methionine
-
Aspergillus sp.
0.1
-
trifluoromethionine
-
Entamoeba histolytica
0.18
-
L-cysteine
-
Citrobacter freundii
0.2
-
L-ethionine
-
Pseudomonas putida
0.27
-
L-cystathionine
-
Lactococcus lactis
0.45
-
L-ethionine
-
Pseudomonas putida
0.46
-
L-cysteine
-
Lactococcus lactis
0.5
-
DL-homocysteine
-
Trichomonas vaginalis
0.5
-
L-ethionine
-
Trichomonas vaginalis
0.53
-
L-cysteine
-
Pseudomonas putida
0.55
-
L-methionine
-
Treponema denticola
0.56
-
L-ethionine
-
Citrobacter freundii
0.61
-
L-methionine
-
Entamoeba histolytica
0.61
-
S-methyl-L-cysteine
-
Citrobacter freundii
0.64
-
L-cysteine
-
Entamoeba histolytica
0.7
-
L-methionine
-
Citrobacter freundii
0.77
-
S-methylcysteine
-
Pseudomonas putida
0.87
-
L-methionine
-
Lactococcus lactis
1.1
-
DL-homocysteine
-
Citrobacter freundii
1.13
-
L-methionine
-
Citrobacter intermedius
1.33
-
L-methionine
-
Pseudomonas putida
1.8
-
DL-homocysteine
-
Pseudomonas putida
3.03
-
DL-homocysteine
-
Entamoeba histolytica
3.6
-
L-cysteine
-
Trichomonas vaginalis
4.3
-
L-methionine
-
Trichomonas vaginalis
6.43
-
L-homocysteine
-
Pseudomonas putida
30
-
L-homoserine
-
Pseudomonas putida
90
-
L-methionine
-
Clostridium sporogenes
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
intracellular
-
Achromobacter starkeyi
5622
-
intracellular
-
Aeromonas sp.
5622
-
intracellular
-
Aspergillus flavipes
5622
-
intracellular
-
Aspergillus sp.
5622
-
intracellular
-
Brevibacterium linens BL2
5622
-
intracellular
-
Citrobacter freundii
5622
-
intracellular
-
Citrobacter intermedius
5622
-
intracellular
-
Cladosporium cladosporioides
5622
-
intracellular
-
Clostridium sporogenes
5622
-
intracellular
-
Entamoeba histolytica
5622
-
intracellular
-
Lactococcus lactis
5622
-
intracellular
-
Pseudomonas putida
5622
-
intracellular
-
Treponema denticola
5622
-
intracellular
-
Trichomonas vaginalis
5622
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
significant enzyme inhibition
Aspergillus flavipes
Co2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Cu2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Li+
significant enzyme inhibition
Aspergillus flavipes
Mg2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Mn2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Na+
significant enzyme inhibition
Aspergillus flavipes
Ni2+
slightly stimulatory effect on the enzyme activity
Aspergillus flavipes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
4 * 37500, SDS-PAGE
Clostridium sporogenes
41000
-
4 * 41000, SDS-PAGE
Aeromonas sp.
42600
-
4 * 42600, SDS-PAGE
Pseudomonas putida
43000
-
4 * 43000, SDS-PAGE
Brevibacterium linens BL2
43000
-
4 * 43000, SDS-PAGE
Citrobacter freundii
43000
-
4 * 43000, SDS-PAGE
Entamoeba histolytica
44000
-
4 * 44000, SDS-PAGE
Trichomonas vaginalis
45000
-
4 * 45000, SDS-PAGE
Pseudomonas putida
55000
-
4 * 55000, SDS-PAGE
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
ion exchange and gel filtration chromatography
Aeromonas sp.
ion exchange and gel filtration chromatography
Aspergillus flavipes
ion exchange and gel filtration chromatography
Aspergillus sp.
ion exchange and gel filtration chromatography
Brevibacterium linens BL2
ion exchange and gel filtration chromatography
Citrobacter freundii
ion exchange and gel filtration chromatography
Citrobacter intermedius
ion exchange and gel filtration chromatography
Cladosporium cladosporioides
ion exchange and gel filtration chromatography
Clostridium sporogenes
ion exchange and gel filtration chromatography
Entamoeba histolytica
ion exchange and gel filtration chromatography
Lactococcus lactis
ion exchange and gel filtration chromatography
Pseudomonas putida
ion exchange and gel filtration chromatography
Treponema denticola
ion exchange and gel filtration chromatography
Trichomonas vaginalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
value is 31.0 U/mg protein
Aeromonas sp.
additional information
-
value is 61 U/mg protein
Aspergillus flavipes
additional information
-
value is 44.8 U/mg protein
Aspergillus sp.
additional information
-
value is 228.9 U/mg protein
Brevibacterium linens BL2
additional information
-
value is 10 U/mg protein
Citrobacter freundii
additional information
-
value is 0.26 U/mg protein
Citrobacter intermedius
additional information
-
value is 0.44 U/mg protein
Entamoeba histolytica
additional information
-
value is 0.04 U/mg protein
Lactococcus lactis
additional information
-
value is 1.8 U/mg protein; value is 45.3 U/mg protein
Pseudomonas putida
additional information
-
value is 36.6 U/mg protein
Treponema denticola
additional information
-
value is 3.17 U/mg protein
Trichomonas vaginalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cystathionine + ?
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-ethionine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-homocysteic acid + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
DL-homocysteine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-cystathionine + ?
-
701880
Lactococcus lactis
?
-
-
-
?
L-cysteine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
L-cysteine + H2O
-
701880
Lactococcus lactis
?
-
-
-
?
L-cysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-cysteine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-cysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-cysteine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-cysteine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
L-ethionine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-ethionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-ethionine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-ethionine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-ethionine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-ethionine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-ethionine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-homocysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-homocysteine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-homoserine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-homoserine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-homoserine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-methionine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
L-methionine + H2O
-
701880
Lactococcus lactis
?
-
-
-
?
L-methionine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
L-methionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
L-methionine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
L-methionine + H2O
-
701880
Citrobacter intermedius
?
-
-
-
?
L-methionine + H2O
-
701880
Clostridium sporogenes
?
-
-
-
?
L-methionine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus sp.
?
-
-
-
?
L-methionine + H2O
-
701880
Treponema denticola
?
-
-
-
?
L-methionine + H2O
-
701880
Cladosporium cladosporioides
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus flavipes
?
-
-
-
?
L-methionine + H2O
-
701880
Brevibacterium linens BL2
?
-
-
-
?
L-methionine + H2O
-
701880
Aspergillus sp. Rs-1a
?
-
-
-
?
L-selenomethionine + H2O
-
701880
Trichomonas vaginalis
?
-
-
-
?
O-acetyl-L-homoserine + H2O
-
701880
Aeromonas sp.
?
-
-
-
?
S-methyl-L-cysteine + H2O
-
701880
Citrobacter freundii
?
-
-
-
?
S-methylcysteine + H2O
-
701880
Pseudomonas putida
?
-
-
-
?
trifluoromethionine + H2O
-
701880
Entamoeba histolytica
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Achromobacter starkeyi
tetramer
4 * 41000, SDS-PAGE
Aeromonas sp.
tetramer
-
Aspergillus flavipes
tetramer
-
Aspergillus sp.
tetramer
4 * 43000, SDS-PAGE
Brevibacterium linens BL2
tetramer
4 * 43000, SDS-PAGE
Citrobacter freundii
tetramer
-
Citrobacter intermedius
tetramer
-
Cladosporium cladosporioides
tetramer
4 * 37500, SDS-PAGE
Clostridium sporogenes
tetramer
4 * 43000, SDS-PAGE
Entamoeba histolytica
tetramer
4 * 55000, SDS-PAGE
Lactococcus lactis
tetramer
4 * 42600, SDS-PAGE; 4 * 45000, SDS-PAGE
Pseudomonas putida
tetramer
4*43500, SDS-PAGE
Treponema denticola
tetramer
4 * 44000, SDS-PAGE
Trichomonas vaginalis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Brevibacterium linens BL2
30
-
-
Aspergillus flavipes
30
-
-
Citrobacter freundii
30
-
-
Citrobacter intermedius
30
-
-
Cladosporium cladosporioides
30
-
-
Lactococcus lactis
35
-
-
Aspergillus sp.
37
-
-
Entamoeba histolytica
37
-
-
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
L-methionine
values between 25.3 to 48.6, depending on publications
Pseudomonas putida
0.81
-
trifluoromethionine
-
Entamoeba histolytica
1.4
-
L-homoserine
-
Pseudomonas putida
1.59
-
L-cysteine
-
Entamoeba histolytica
1.82
-
L-methionine
-
Entamoeba histolytica
2.27
-
L-cysteine
-
Pseudomonas putida
2.3
-
L-cysteine
-
Citrobacter freundii
3.92
-
DL-homocysteine
-
Entamoeba histolytica
5.1
-
DL-homocysteine
-
Citrobacter freundii
5.9
-
S-methyl-L-cysteine
-
Citrobacter freundii
6.2
-
L-ethionine
-
Citrobacter freundii
6.5
-
L-methionine
-
Citrobacter freundii
33.4
-
L-ethionine
-
Pseudomonas putida
44.8
-
DL-homocysteine
-
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Entamoeba histolytica
7.2
-
-
Pseudomonas putida
7.5
-
-
Brevibacterium linens BL2
8
-
-
Aeromonas sp.
8
-
-
Aspergillus flavipes
8
-
-
Aspergillus sp.
8
-
-
Citrobacter freundii
8
-
-
Pseudomonas putida
9
-
-
Cladosporium cladosporioides
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8
-
Trichomonas vaginalis
7.5
8.5
-
Clostridium sporogenes
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.6
8.2
-
Entamoeba histolytica
6
10
-
Pseudomonas putida
6
8
-
Brevibacterium linens BL2
6
9
-
Aeromonas sp.
6
9
-
Pseudomonas putida
6.5
9
-
Trichomonas vaginalis
8
9
-
Aspergillus flavipes
8
9
-
Aspergillus sp.
Other publictions for EC 4.4.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747200
Raboni
Engineering methionine gamma- ...
Citrobacter freundii
Biochim. Biophys. Acta
1866
1260-1270
2018
-
1
1
1
5
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
1
1
-
1
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
747211
Kulikova
Non-stereoselective decomposi ...
Citrobacter freundii
Biochimie
151
42-44
2018
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748439
Morozova
Soluble and nanoporous silica ...
Citrobacter freundii
J. Nanosci. Nanotechnol.
18
2210-2219
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747194
Revtovich
Crystal structure of mutant f ...
Citrobacter freundii
Biochim. Biophys. Acta
1865
1123-1128
2017
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747242
Morozova
Plasma methionine depletion a ...
Citrobacter freundii, Clostridium sporogenes, Clostridium sporogenes ATCC 15579, Clostridium tetani
Biomed. Pharmacother.
88
978-984
2017
-
3
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748010
Kumar
Concurrent overexpression of ...
Solanum tuberosum
J. Agric. Food Chem.
65
2737-2742
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749242
Sato
Structural and mechanistic in ...
Pseudomonas putida
Protein Sci.
26
1224-1230
2017
-
-
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749397
Lin
-
Mechanistic insights into the ...
Citrobacter freundii, Clostridium sporogenes, Clostridium sporogenes ATCC 15579
Theoret. Chem. Accounts
136
105
2017
-
-
-
2
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746664
Revtovich
Structure of methionine gamma ...
Clostridium sporogenes, Clostridium sporogenes ATCC 15579
Acta Crystallogr. Sect. F
72
65-71
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746734
Foo
A continuous spectrophotometr ...
Porphyromonas gingivalis
Anal. Biochem.
507
21-26
2016
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
747207
Morozova
Engineered Citrobacter freund ...
Citrobacter freundii
Biochimie
128-129
92-98
2016
-
-
1
-
2
-
-
15
-
-
-
-
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
15
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
15
-
-
-
-
-
-
-
-
15
15
749099
Stephen
In vitro effect of Porphyromo ...
Porphyromonas gingivalis
PLoS ONE
11
e0169157
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749380
Cao
Structural dynamics of a meth ...
Micromonospora echinospora
Struct. Dyn.
3
034702
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747172
Anufrieva
The role of active site tyros ...
Citrobacter freundii
Biochim. Biophys. Acta
1854
1220-1228
2015
-
-
-
1
1
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
1
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
747532
Song
Identification and characteri ...
Micromonospora echinospora
ChemBioChem
16
100-109
2015
-
-
1
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
748205
Kuznetsov
Pre-steady-state kinetic and ...
Citrobacter freundii
J. Biol. Chem.
290
671-681
2015
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748253
Kudou
Molecular cloning and charact ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
J. Biosci. Bioeng.
120
380-383
2015
-
-
1
-
-
-
-
3
-
-
-
-
-
5
-
-
1
-
-
-
-
-
6
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
6
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
748528
Huang
High-level expression, purifi ...
Idiomarina sp.
Mar. Drugs
13
5492-5507
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729320
Revtovich
Crystal structure of the exter ...
Citrobacter freundii
Biochimie
101
161-167
2014
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730581
Huang
The catabolic enzyme methionin ...
Solanum tuberosum
Plant Biotechnol. J.
12
883-893
2014
-
-
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
728927
Morozova
Kinetic parameters and cytotox ...
Citrobacter freundii, Clostridium sporogenes, Clostridium tetani, Porphyromonas gingivalis
Acta Naturae
5
92-98
2013
-
3
4
-
-
-
-
19
-
-
7
4
-
4
-
-
4
-
-
-
4
-
24
4
4
-
-
15
4
-
-
4
-
-
-
-
3
4
4
-
-
-
-
-
-
19
-
-
7
4
-
-
-
4
-
-
4
-
24
4
4
-
-
15
4
-
-
-
-
-
-
-
15
15
730925
Khan
-
Activities of methionine-gamma ...
Ferroplasma acidarmanus, Ferroplasma acidarmanus Fer1
Res. Rep. Biol.
4
11-22
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728952
Saa
Assays for methionine beta-lya ...
Pseudomonas putida, Pseudomonas putida ICR 3460
Anal. Chem.
84
8961-8965
2012
-
1
-
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
1
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
729426
Fukumoto
The role of amino acid residue ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
76
1275-1284
2012
-
-
1
1
16
-
1
18
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
1
-
4
19
1
-
-
1
-
-
-
-
-
1
1
1
16
-
-
1
-
18
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
4
19
1
-
-
-
-
1
1
-
23
23
714356
Ronda
Exploring methionine gamma-lya ...
Citrobacter freundii
Biochim. Biophys. Acta
1814
834-842
2011
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716146
Suwabe
Identification of an L-methion ...
Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Treponema denticola, Treponema denticola ATCC 35405
Microbiology
157
2992-3000
2011
-
-
3
-
-
-
-
3
-
-
8
-
-
8
-
-
-
-
-
-
-
-
12
3
-
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
8
-
-
-
-
-
-
-
-
-
12
3
-
-
-
3
-
-
-
-
-
1
1
-
3
3
729181
Revtovich
Three-dimensional structures o ...
Citrobacter freundii
Biochemistry (Moscow)
76
564-570
2011
-
-
-
1
-
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
1
-
-
-
1
1
3
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
701656
Joshi
Interdependence of threonine, ...
Arabidopsis thaliana
Amino Acids
39
933-947
2010
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
701880
El-Sayed
Microbial L-methioninase: prod ...
Achromobacter starkeyi, Aeromonas sp., Aspergillus flavipes, Aspergillus sp., Aspergillus sp. Rs-1a, Brevibacterium linens BL2, Citrobacter freundii, Citrobacter intermedius, Cladosporium cladosporioides, Clostridium sporogenes, Entamoeba histolytica, Lactococcus lactis, no activity in mammalia, Pseudomonas putida, Treponema denticola, Trichomonas vaginalis
Appl. Microbiol. Biotechnol.
86
445-467
2010
-
14
-
13
-
-
56
29
14
8
9
-
-
17
-
-
13
-
-
-
11
-
50
14
9
-
-
14
9
2
8
14
-
-
-
-
14
-
14
13
-
-
-
56
-
29
14
8
9
-
-
-
-
13
-
-
11
-
50
14
9
-
-
14
9
2
8
-
-
-
-
-
-
-
704038
Sato
Cytotoxic effect of amide deri ...
Entamoeba histolytica
Int. J. Antimicrob. Agents
35
56-61
2010
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1
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714123
Morozova
Kinetic and spectral parameter ...
Citrobacter freundii
Biochemistry (Moscow)
75
1272-1280
2010
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1
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7
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7
691957
Khalaf
L-methioninase production by f ...
Aspergillus carneus, Aspergillus flavipes, Aspergillus flavus, Aspergillus niger, Aspergillus ochraceus, Aspergillus oryzae, Aspergillus parasiticus, Aspergillus subolivaceus, Aspergillus tamarii, Cladosporium oxysporum, Fusarium oxysporum, Fusarium solani, Humicola fuscoatra, Microdochium nivale, Mucor racemosus, Penicillium chrysogenum, Penicillium citrinum, Penicillium digitatum, Penicillium egyptiacum, Scopulariopsis brevicaulis, Trichoderma koningii
Curr. Microbiol.
58
219-226
2009
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692665
Hu
Methionine depletion with reco ...
Pseudomonas putida
Int. J. Cancer
124
1700-1706
2009
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701789
Hanniffy
Heterologous production of met ...
Brevibacterium linens BL2
Appl. Environ. Microbiol.
75
2326-2332
2009
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1
1
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6
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1
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702447
Faleev
Methionine gamma-lyase: mechan ...
Citrobacter freundii
Biochim. Biophys. Acta
1794
1414-1420
2009
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1
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704116
Sato
Methionine gamma-lyase: the un ...
Arabidopsis thaliana, Brevibacterium linens, Entamoeba histolytica, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella denticola, Pseudomonas putida, Trichomonas vaginalis
IUBMB Life
61
1019-1028
2009
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8
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8
1
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3
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13
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1
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3
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2
2
2
2
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704336
El-Sayed
L-methioninase production by A ...
Aspergillus flavipes
J. Basic Microbiol.
49
331-341
2009
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10
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10
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1
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1
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706098
Palwai
Selective growth inhibition of ...
Escherichia coli BL21
Pharmacology
84
271-275
2009
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1
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2
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706315
Joshi
Arabidopsis methionine gamma-l ...
Arabidopsis thaliana
Plant Physiol.
151
367-378
2009
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1
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1
1
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677329
Nikulin
High-resolution structure of m ...
Citrobacter freundii
Acta Crystallogr. Sect. D
64
211-218
2008
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679804
Sato
Kinetic characterization of me ...
Entamoeba histolytica
FEBS J.
275
548-560
2008
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8
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21
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7
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12
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24
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690268
Sato
Crystallization and preliminar ...
Entamoeba histolytica
Acta Crystallogr. Sect. F
64
697-699
2008
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1
1
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1
1
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691377
Kudou
The role of cysteine 116 in th ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
72
1722-1730
2008
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1
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19
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1
12
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2
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1
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12
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19
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10
1
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12
-
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-
691380
Ito
Identification of a methionina ...
Fusobacterium nucleatum, Fusobacterium nucleatum JCM 8532, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Treponema denticola, Treponema denticola ATCC 35405
Biosci. Biotechnol. Biochem.
72
2411-2414
2008
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3
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18
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3
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3
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6
-
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694318
Han
Enzymatic assay for total plas ...
Pseudomonas putida
Nat. Protoc.
3
1778-1781
2008
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4
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1
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680553
Kudou
Structure of the antitumour en ...
Pseudomonas putida
J. Biochem.
141
535-544
2007
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1
9
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2
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681033
Kim
Methylselenol generated from s ...
Mus musculus
J. Cell. Physiol.
212
386-400
2007
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1
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682347
Goyer
Functional characterization of ...
Arabidopsis thaliana
Plant Cell Physiol.
48
232-242
2007
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1
1
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1
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3
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5
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660779
Takakura
High-level expression and bulk ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
70
183-192
2006
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1
1
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1
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2
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1
1
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661430
Takakura
Physicochemical and pharmacoki ...
Mus musculus
Cancer Res.
66
2807-2814
2006
-
1
-
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1
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5
1
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2
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1
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4
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1
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1
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1
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1
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1
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5
1
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1
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4
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1
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-
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-
677392
Sato
Expression, purification and c ...
Entamoeba histolytica
Acta Crystallogr. Sect. F
F62
1034-1036
2006
-
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1
1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
677670
Martinez-Cuesta
YtjE from Lactococcus lactis I ...
Lactococcus lactis, Lactococcus lactis IL1403
Appl. Environ. Microbiol.
72
4878-4884
2006
1
-
1
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4
-
-
-
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10
-
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4
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1
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1
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4
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-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678355
Manukhov
L-methionine gamma-lyase from ...
Citrobacter freundii
Biochemistry (Moscow)
71
361-369
2006
-
1
1
-
-
-
-
9
-
-
-
-
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5
-
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1
-
-
-
-
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9
-
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9
-
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1
1
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9
-
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1
-
-
-
-
9
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
681870
Zhao
Apoptosis induced by selenomet ...
Homo sapiens
Mol. Cancer Ther.
5
3275-3284
2006
-
1
-
-
-
-
-
-
-
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2
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4
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1
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4
-
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-
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-
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-
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-
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-
-
-
-
682514
Rebeille
Methionine catabolism in Arabi ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
103
15687-15692
2006
-
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1
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-
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1
1
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2
1
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1
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1
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1
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1
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1
1
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2
1
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1
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-
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2
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
660621
Mamaeva
Structure of Citrobacter freun ...
Citrobacter freundii
Acta Crystallogr. Sect. F
61
546-549
2005
-
-
-
1
-
-
-
-
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1
1
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4
-
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-
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1
1
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1
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1
1
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1
1
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1
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
660904
Fukamachi
High production of methyl merc ...
Porphyromonas gingivalis, Treponema denticola
Biochem. Biophys. Res. Commun.
331
127-131
2005
-
1
1
-
-
-
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1
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1
2
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3
-
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1
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1
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3
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1
1
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1
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2
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1
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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662034
Manukhov
A gene encoding L-methionine g ...
Citrobacter freundii, Citrobacter freundii ATCC 29063
J. Bacteriol.
187
3889-3893
2005
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1
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13
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2
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8
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1
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1
1
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12
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-
-
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14
-
-
-
-
-
-
-
-
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1
-
-
-
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13
-
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1
2
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1
-
1
1
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12
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
649323
Takakura
Assay method for antitumor L-m ...
Pseudomonas putida
Anal. Biochem.
327
233-240
2004
-
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1
-
-
-
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3
-
-
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1
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1
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Characterization of L-methioni ...
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649139
Sridhar
Crystallization and preliminar ...
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Inoue
Role of tyrosine 114 of L-meth ...
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Crystal structure of the pyrid ...
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34532
Tan
Polyethylene glycol conjugatio ...
Pseudomonas putida
Protein Expr. Purif.
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1998
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34533
McKie
The primitive protozoon Tricho ...
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34534
Dias
Purification and characterizat ...
Brevibacterium linens
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1998
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34536
Yoshioka
Anticancer efficacy in vivo an ...
Pseudomonas putida
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34537
Tan
Overexpression and large-scale ...
Pseudomonas putida
Protein Expr. Purif.
9
233-245
1997
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34530
Faleev
-
L-methionine-gamma-lyase in Ci ...
Citrobacter intermedius
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590-593
1996
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34535
Hori
Gene cloning and characterizat ...
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Cancer Res.
56
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34531
Lockwood
Purification and characterizat ...
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34515
Esaki
-
Mechanism-based inactivation o ...
Pseudomonas putida
Biochemistry
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34516
Takada
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The C3-N bond cleavage of 2-am ...
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34517
Nakayama
-
Chemical modification of cyste ...
Pseudomonas putida
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34524
Furuyoshi
-
Catalytic action of L-methioni ...
Pseudomonas putida
Agric. Biol. Chem.
51
3159-3160
1987
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34529
Esaki
L-methionine gamma-lyase from ...
Aeromonas sp., Pseudomonas putida
Methods Enzymol.
143
459-465
1987
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2
14
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2
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34523
Tanaka
-
A versatile bacterial enzyme: ...
Aeromonas sp., Pseudomonas putida
Enzyme Microb. Technol.
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530-537
1985
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2
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2
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8
2
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34525
Chocat
Synthesis of L-selenodjenkolat ...
Pseudomonas putida
Anal. Biochem.
148
485-489
1985
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1
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34521
Nakayama
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Purification and properties of ...
Aeromonas sp.
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48
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34522
Nakayama
Purification of bacterial L-me ...
Pseudomonas putida
Anal. Biochem.
138
421-424
1984
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1
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1
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34526
Soda
-
Multifunctional biocatalysis: ...
More, Pseudomonas putida
Trends Biochem. Sci.
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1983
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1
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1
1
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3
2
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4
1
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34528
Tanaka
-
Determination of L-cysteine wi ...
Pseudomonas putida
Agric. Biol. Chem.
45
1021-1022
1981
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1
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1
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34520
Tanaka
Properties of L-methionine gam ...
Pseudomonas putida
Biochemistry
16
100-106
1977
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1
3
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3
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2
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1
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1
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1
4
1
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1
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1
1
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1
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1
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3
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3
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1
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1
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1
4
1
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1
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1
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34518
Tanaka
Purification and properties of ...
Pseudomonas putida
FEBS Lett.
66
307-311
1976
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4
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1
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4
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1
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1
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1
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34519
Ito
Purification and characterizat ...
Pseudomonas putida
J. Biochem.
79
1263-1272
1976
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7
4
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1
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2
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1
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1
1
2
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2
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1
1
2
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7
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4
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1
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1
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1
1
2
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2
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1
1
2
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34527
Ito
Exchange reactions catalyzed b ...
Pseudomonas putida
J. Biochem.
78
1105-1107
1975
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