BRENDA - Enzyme Database
show all sequences of 4.4.1.11

Kinetic parameters and cytotoxic activity of recombinant methionine gamma-lyase from Clostridium tetani, Clostridium sporogenes, Porphyromonas gingivalis and Citrobacter freundii

Morozova, E.A.; Kulikova, V.V.; Yashin, D.V.; Anufrieva, N.V.; Anisimova, N.Y.; Revtovich, S.V.; Kotlov, M.I.; Belyi, Y.F.; Pokrovsky, V.S.; Demidkina, T.V.; Acta Naturae 5, 92-98 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the nezyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Citrobacter freundii
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Clostridium sporogenes
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the nezyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Clostridium tetani
Cloned(Commentary)
Commentary
Organism
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Clostridium sporogenes
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Clostridium tetani
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Porphyromonas gingivalis
recombinant enzyme expression in Escherichia coli strain BL21
Citrobacter freundii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Citrobacter freundii
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Clostridium sporogenes
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Clostridium tetani
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Porphyromonas gingivalis
0.278
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.348
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.358
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.432
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.545
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.72
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.766
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.93
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
0.947
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
1.47
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
1.77
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
2.17
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
7.07
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
12.22
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
33.51
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44040
-
4 * 44040, His-tagged enzyme, sequence calcualtion
Clostridium tetani
44080
-
4 * 44080, His-tagged enzyme, sequence calcualtion
Porphyromonas gingivalis
44360
-
4 * 44360, His-tagged enzyme, sequence calcualtion
Clostridium sporogenes
170000
-
about, recombinant detagged enzyme, gel filtration
Clostridium sporogenes
170000
-
about, recombinant detagged enzyme, gel filtration
Clostridium tetani
170000
-
about, recombinant detagged enzyme, gel filtration
Porphyromonas gingivalis
258000
-
native enzyme, gel filtration
Clostridium tetani
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-methionine + H2O
Citrobacter freundii
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Clostridium sporogenes
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Clostridium tetani
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Porphyromonas gingivalis
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrobacter freundii
-
-
-
Clostridium sporogenes
-
-
-
Clostridium tetani
-
-
-
Porphyromonas gingivalis
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21
Citrobacter freundii
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Clostridium sporogenes
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Clostridium tetani
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Porphyromonas gingivalis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Porphyromonas gingivalis
10.2
-
purified recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Citrobacter freundii
12.8
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Clostridium sporogenes
16.6
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Clostridium tetani
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine + H2O
gamma-elimination reaction
728927
Citrobacter freundii
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Clostridium tetani
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Citrobacter freundii
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Clostridium sporogenes
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Clostridium tetani
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Porphyromonas gingivalis
?
-
-
-
-
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
dimer of dimers, 2 active sites, structure model
Citrobacter freundii
tetramer
4 * 44360, His-tagged enzyme, sequence calcualtion
Clostridium sporogenes
tetramer
4 * 44040, His-tagged enzyme, sequence calcualtion
Clostridium tetani
tetramer
4 * 44080, His-tagged enzyme, sequence calcualtion
Porphyromonas gingivalis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Citrobacter freundii
30
-
assay at
Clostridium sporogenes
30
-
assay at
Clostridium tetani
30
-
assay at
Porphyromonas gingivalis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
3.84
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.9
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.05
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.8
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.89
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
6.3
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
6.7
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
7.05
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
7.08
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
8.05
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
8.5
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
9.86
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
10
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
12
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Citrobacter freundii
8
-
assay at
Clostridium sporogenes
8
-
assay at
Clostridium tetani
8
-
assay at
Porphyromonas gingivalis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Clostridium sporogenes
pyridoxal 5'-phosphate
-
Clostridium tetani
pyridoxal 5'-phosphate
-
Porphyromonas gingivalis
pyridoxal 5'-phosphate
-
Citrobacter freundii
Application (protein specific)
Application
Commentary
Organism
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the nezyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Citrobacter freundii
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the enzyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Clostridium sporogenes
medicine
the enzyme is of interest as an anticancer agent, since the growth of malignant cells of various origins (unlike the growth of normal cells) is accompanied by obligatory methionine utilization, and the nezyme is absent in mammalian cells. IC50 of MGL for several tumor cell cultures, overview
Clostridium tetani
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Clostridium sporogenes
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Clostridium tetani
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Porphyromonas gingivalis
recombinant enzyme expression in Escherichia coli strain BL21
Citrobacter freundii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Citrobacter freundii
pyridoxal 5'-phosphate
-
Clostridium sporogenes
pyridoxal 5'-phosphate
-
Clostridium tetani
pyridoxal 5'-phosphate
-
Porphyromonas gingivalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Citrobacter freundii
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Clostridium sporogenes
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Clostridium tetani
additional information
-
additional information
steady-state kinetics for beta- and gamma-elimination reactions, overview
Porphyromonas gingivalis
0.278
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.348
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.358
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.432
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.545
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.72
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.766
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.93
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
0.947
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
1.47
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
1.77
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
2.17
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
7.07
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
12.22
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
33.51
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44040
-
4 * 44040, His-tagged enzyme, sequence calcualtion
Clostridium tetani
44080
-
4 * 44080, His-tagged enzyme, sequence calcualtion
Porphyromonas gingivalis
44360
-
4 * 44360, His-tagged enzyme, sequence calcualtion
Clostridium sporogenes
170000
-
about, recombinant detagged enzyme, gel filtration
Clostridium sporogenes
170000
-
about, recombinant detagged enzyme, gel filtration
Clostridium tetani
170000
-
about, recombinant detagged enzyme, gel filtration
Porphyromonas gingivalis
258000
-
native enzyme, gel filtration
Clostridium tetani
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-methionine + H2O
Citrobacter freundii
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Clostridium sporogenes
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Clostridium tetani
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
L-methionine + H2O
Porphyromonas gingivalis
gamma-elimination reaction
methanethiol + NH3 + 2-oxobutanoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21
Citrobacter freundii
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Clostridium sporogenes
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Clostridium tetani
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag by thrombin and elimination by gel filtration
Porphyromonas gingivalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Porphyromonas gingivalis
10.2
-
purified recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Citrobacter freundii
12.8
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Clostridium sporogenes
16.6
-
purified His-tagged recombinant enzyme, pH 8.0, 30°C, substrate L-methionine
Clostridium tetani
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-methionine + H2O
gamma-elimination reaction
728927
Citrobacter freundii
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Clostridium tetani
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
methanethiol + NH3 + 2-oxobutanoate
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
L-methionine sulfoxide + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Citrobacter freundii
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Clostridium sporogenes
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Clostridium tetani
?
-
-
-
-
additional information
substrate specificty, overview. In addition to the physiological reaction, the enzyme catalyzes the beta-elimination reaction of L-cysteine and its S-substituted derivatives, yielding the corresponding mercaptans, pyruvic acid, and ammonia
728927
Porphyromonas gingivalis
?
-
-
-
-
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-benzyl-L-cysteine + H2O
beta-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-ethyl-L-cysteine + H2O
beta-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Citrobacter freundii
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Clostridium sporogenes
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Clostridium tetani
?
-
-
-
?
S-ethyl-L-homocysteine + H2O
gamma-elimination reaction
728927
Porphyromonas gingivalis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
dimer of dimers, 2 active sites, structure model
Citrobacter freundii
tetramer
4 * 44360, His-tagged enzyme, sequence calcualtion
Clostridium sporogenes
tetramer
4 * 44040, His-tagged enzyme, sequence calcualtion
Clostridium tetani
tetramer
4 * 44080, His-tagged enzyme, sequence calcualtion
Porphyromonas gingivalis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Citrobacter freundii
30
-
assay at
Clostridium sporogenes
30
-
assay at
Clostridium tetani
30
-
assay at
Porphyromonas gingivalis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
3.84
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.9
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.05
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.8
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
5.89
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
6.3
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
6.7
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
7.05
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
7.08
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
8.05
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
8.5
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
9.86
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
10
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
12
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Citrobacter freundii
8
-
assay at
Clostridium sporogenes
8
-
assay at
Clostridium tetani
8
-
assay at
Porphyromonas gingivalis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.382
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.413
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
2.2
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.71
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.94
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
4.13
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
9.83
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
10.8
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
11.1
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
12.7
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
17.6
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
22.8
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
25.4
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
28.7
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.2
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
0.382
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
0.413
-
L-methionine sulfoxide
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
2.2
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.71
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
3.94
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
4.13
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Porphyromonas gingivalis
9.83
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
10.8
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
11.1
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
12.7
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium tetani
17.6
-
S-ethyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
22.8
-
L-methionine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
25.4
-
S-ethyl-L-homocysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
28.7
-
S-Benzyl-L-cysteine
pH 8.0, 30°C, recombinant enzyme
Clostridium sporogenes
Other publictions for EC 4.4.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747200
Raboni
Engineering methionine gamma- ...
Citrobacter freundii
Biochim. Biophys. Acta
1866
1260-1270
2018
-
1
1
1
5
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
1
1
-
1
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
747211
Kulikova
Non-stereoselective decomposi ...
Citrobacter freundii
Biochimie
151
42-44
2018
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748439
Morozova
Soluble and nanoporous silica ...
Citrobacter freundii
J. Nanosci. Nanotechnol.
18
2210-2219
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747194
Revtovich
Crystal structure of mutant f ...
Citrobacter freundii
Biochim. Biophys. Acta
1865
1123-1128
2017
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747242
Morozova
Plasma methionine depletion a ...
Citrobacter freundii, Clostridium sporogenes, Clostridium sporogenes ATCC 15579, Clostridium tetani
Biomed. Pharmacother.
88
978-984
2017
-
3
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748010
Kumar
Concurrent overexpression of ...
Solanum tuberosum
J. Agric. Food Chem.
65
2737-2742
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749242
Sato
Structural and mechanistic in ...
Pseudomonas putida
Protein Sci.
26
1224-1230
2017
-
-
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749397
Lin
-
Mechanistic insights into the ...
Citrobacter freundii, Clostridium sporogenes, Clostridium sporogenes ATCC 15579
Theoret. Chem. Accounts
136
105
2017
-
-
-
2
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746664
Revtovich
Structure of methionine gamma ...
Clostridium sporogenes, Clostridium sporogenes ATCC 15579
Acta Crystallogr. Sect. F
72
65-71
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746734
Foo
A continuous spectrophotometr ...
Porphyromonas gingivalis
Anal. Biochem.
507
21-26
2016
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
747207
Morozova
Engineered Citrobacter freund ...
Citrobacter freundii
Biochimie
128-129
92-98
2016
-
-
1
-
2
-
-
15
-
-
-
-
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
15
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
15
-
-
-
-
-
-
-
-
15
15
749099
Stephen
In vitro effect of Porphyromo ...
Porphyromonas gingivalis
PLoS ONE
11
e0169157
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749380
Cao
Structural dynamics of a meth ...
Micromonospora echinospora
Struct. Dyn.
3
034702
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747172
Anufrieva
The role of active site tyros ...
Citrobacter freundii
Biochim. Biophys. Acta
1854
1220-1228
2015
-
-
-
1
1
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
1
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
747532
Song
Identification and characteri ...
Micromonospora echinospora
ChemBioChem
16
100-109
2015
-
-
1
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
748205
Kuznetsov
Pre-steady-state kinetic and ...
Citrobacter freundii
J. Biol. Chem.
290
671-681
2015
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748253
Kudou
Molecular cloning and charact ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
J. Biosci. Bioeng.
120
380-383
2015
-
-
1
-
-
-
-
3
-
-
-
-
-
5
-
-
1
-
-
-
-
-
6
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
6
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
748528
Huang
High-level expression, purifi ...
Idiomarina sp.
Mar. Drugs
13
5492-5507
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729320
Revtovich
Crystal structure of the exter ...
Citrobacter freundii
Biochimie
101
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2014
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The catabolic enzyme methionin ...
Solanum tuberosum
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2
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728927
Morozova
Kinetic parameters and cytotox ...
Citrobacter freundii, Clostridium sporogenes, Clostridium tetani, Porphyromonas gingivalis
Acta Naturae
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2013
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Activities of methionine-gamma ...
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2013
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Assays for methionine beta-lya ...
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2012
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729426
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The role of amino acid residue ...
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2012
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714356
Ronda
Exploring methionine gamma-lya ...
Citrobacter freundii
Biochim. Biophys. Acta
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2011
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716146
Suwabe
Identification of an L-methion ...
Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Treponema denticola, Treponema denticola ATCC 35405
Microbiology
157
2992-3000
2011
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3
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3
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1
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3
3
729181
Revtovich
Three-dimensional structures o ...
Citrobacter freundii
Biochemistry (Moscow)
76
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2011
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2
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701656
Joshi
Interdependence of threonine, ...
Arabidopsis thaliana
Amino Acids
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2010
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701880
El-Sayed
Microbial L-methioninase: prod ...
Achromobacter starkeyi, Aeromonas sp., Aspergillus flavipes, Aspergillus sp., Aspergillus sp. Rs-1a, Brevibacterium linens BL2, Citrobacter freundii, Citrobacter intermedius, Cladosporium cladosporioides, Clostridium sporogenes, Entamoeba histolytica, Lactococcus lactis, no activity in mammalia, Pseudomonas putida, Treponema denticola, Trichomonas vaginalis
Appl. Microbiol. Biotechnol.
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445-467
2010
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9
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2
8
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704038
Sato
Cytotoxic effect of amide deri ...
Entamoeba histolytica
Int. J. Antimicrob. Agents
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56-61
2010
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714123
Morozova
Kinetic and spectral parameter ...
Citrobacter freundii
Biochemistry (Moscow)
75
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2010
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1
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7
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7
7
691957
Khalaf
L-methioninase production by f ...
Aspergillus carneus, Aspergillus flavipes, Aspergillus flavus, Aspergillus niger, Aspergillus ochraceus, Aspergillus oryzae, Aspergillus parasiticus, Aspergillus subolivaceus, Aspergillus tamarii, Cladosporium oxysporum, Fusarium oxysporum, Fusarium solani, Humicola fuscoatra, Microdochium nivale, Mucor racemosus, Penicillium chrysogenum, Penicillium citrinum, Penicillium digitatum, Penicillium egyptiacum, Scopulariopsis brevicaulis, Trichoderma koningii
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2009
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692665
Hu
Methionine depletion with reco ...
Pseudomonas putida
Int. J. Cancer
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2009
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1
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701789
Hanniffy
Heterologous production of met ...
Brevibacterium linens BL2
Appl. Environ. Microbiol.
75
2326-2332
2009
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1
1
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6
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702447
Faleev
Methionine gamma-lyase: mechan ...
Citrobacter freundii
Biochim. Biophys. Acta
1794
1414-1420
2009
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1
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1
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704116
Sato
Methionine gamma-lyase: the un ...
Arabidopsis thaliana, Brevibacterium linens, Entamoeba histolytica, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella denticola, Pseudomonas putida, Trichomonas vaginalis
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61
1019-1028
2009
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9
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3
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2
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704336
El-Sayed
L-methioninase production by A ...
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2009
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10
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10
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706098
Palwai
Selective growth inhibition of ...
Escherichia coli BL21
Pharmacology
84
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2009
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706315
Joshi
Arabidopsis methionine gamma-l ...
Arabidopsis thaliana
Plant Physiol.
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677329
Nikulin
High-resolution structure of m ...
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679804
Sato
Kinetic characterization of me ...
Entamoeba histolytica
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24
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690268
Sato
Crystallization and preliminar ...
Entamoeba histolytica
Acta Crystallogr. Sect. F
64
697-699
2008
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691377
Kudou
The role of cysteine 116 in th ...
Pseudomonas putida
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72
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1
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12
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691380
Ito
Identification of a methionina ...
Fusobacterium nucleatum, Fusobacterium nucleatum JCM 8532, Porphyromonas gingivalis, Porphyromonas gingivalis W83, Treponema denticola, Treponema denticola ATCC 35405
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3
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6
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Han
Enzymatic assay for total plas ...
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Kudou
Structure of the antitumour en ...
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Functional characterization of ...
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660779
Takakura
High-level expression and bulk ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
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661430
Takakura
Physicochemical and pharmacoki ...
Mus musculus
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677392
Sato
Expression, purification and c ...
Entamoeba histolytica
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1
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YtjE from Lactococcus lactis I ...
Lactococcus lactis, Lactococcus lactis IL1403
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678355
Manukhov
L-methionine gamma-lyase from ...
Citrobacter freundii
Biochemistry (Moscow)
71
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2006
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1
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9
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9
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681870
Zhao
Apoptosis induced by selenomet ...
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Mol. Cancer Ther.
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2006
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Rebeille
Methionine catabolism in Arabi ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
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2006
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Mamaeva
Structure of Citrobacter freun ...
Citrobacter freundii
Acta Crystallogr. Sect. F
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2005
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1
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Fukamachi
High production of methyl merc ...
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Manukhov
A gene encoding L-methionine g ...
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14
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13
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12
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14
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Takakura
Assay method for antitumor L-m ...
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2004
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Spallholz
Methioninase and selenomethion ...
Trichomonas vaginalis
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4
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Amarita
Identification and functional ...
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In vivo efficacy of recombinan ...
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1
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Tokoro
Identification and characteriz ...
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1
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1
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1
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1
4
1
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3
1
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9
1
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1
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649595
Yoshimura
L-Methionine-gamma-lyase, as a ...
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1
1
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653114
Alferov
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A phosphinic analogue of methi ...
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Abu-Seidah
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Characterization of L-methioni ...
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4
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649139
Sridhar
Crystallization and preliminar ...
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1
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1
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1
1
1
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1
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1
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1
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650615
Inoue
Role of tyrosine 114 of L-meth ...
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11
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1
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1
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1
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7
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1
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7
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Motoshima
Crystal structure of the pyrid ...
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1
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Tan
Polyethylene glycol conjugatio ...
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Purification and characterizat ...
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Anticancer efficacy in vivo an ...
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Overexpression and large-scale ...
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1
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1
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2
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34535
Hori
Gene cloning and characterizat ...
Pseudomonas putida
Cancer Res.
56
2116-2122
1996
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1
1
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1
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5
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34531
Lockwood
Purification and characterizat ...
Trichomonas vaginalis
Biochem. J.
279
675-682
1991
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7
2
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1
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3
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1
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1
1
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7
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1
1
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1
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34515
Esaki
-
Mechanism-based inactivation o ...
Pseudomonas putida
Biochemistry
28
2111-2116
1989
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1
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1
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34516
Takada
-
The C3-N bond cleavage of 2-am ...
Pseudomonas putida
Agric. Biol. Chem.
52
2897-2901
1988
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5
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1
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1
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4
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5
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4
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34517
Nakayama
-
Chemical modification of cyste ...
Pseudomonas putida
Agric. Biol. Chem.
52
177-183
1988
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2
2
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1
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1
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1
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34524
Furuyoshi
-
Catalytic action of L-methioni ...
Pseudomonas putida
Agric. Biol. Chem.
51
3159-3160
1987
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-
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1
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1
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1
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1
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34529
Esaki
L-methionine gamma-lyase from ...
Aeromonas sp., Pseudomonas putida
Methods Enzymol.
143
459-465
1987
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-
-
-
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2
14
-
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4
2
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1
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2
1
2
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2
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2
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2
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14
-
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2
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1
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2
1
2
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2
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2
-
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-
34523
Tanaka
-
A versatile bacterial enzyme: ...
Aeromonas sp., Pseudomonas putida
Enzyme Microb. Technol.
7
530-537
1985
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-
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2
2
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6
2
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2
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2
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8
2
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2
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2
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2
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2
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6
2
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8
2
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34525
Chocat
Synthesis of L-selenodjenkolat ...
Pseudomonas putida
Anal. Biochem.
148
485-489
1985
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1
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1
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1
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-
34521
Nakayama
-
Purification and properties of ...
Aeromonas sp.
Agric. Biol. Chem.
48
2367-2369
1984
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-
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4
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2
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1
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1
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1
1
2
1
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1
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1
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1
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4
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2
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1
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1
1
2
1
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1
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1
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1
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34522
Nakayama
Purification of bacterial L-me ...
Pseudomonas putida
Anal. Biochem.
138
421-424
1984
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1
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2
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3
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1
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1
1
1
1
1
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1
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1
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1
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2
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1
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1
1
1
1
1
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34526
Soda
-
Multifunctional biocatalysis: ...
More, Pseudomonas putida
Trends Biochem. Sci.
8
214-217
1983
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1
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3
2
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2
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4
1
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1
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1
1
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3
2
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4
1
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34528
Tanaka
-
Determination of L-cysteine wi ...
Pseudomonas putida
Agric. Biol. Chem.
45
1021-1022
1981
-
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1
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1
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1
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34520
Tanaka
Properties of L-methionine gam ...
Pseudomonas putida
Biochemistry
16
100-106
1977
-
-
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1
3
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3
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2
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1
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1
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1
4
1
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1
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1
1
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1
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1
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3
-
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3
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1
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1
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1
4
1
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1
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1
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-
34518
Tanaka
Purification and properties of ...
Pseudomonas putida
FEBS Lett.
66
307-311
1976
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-
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4
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1
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2
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1
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1
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2
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1
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1
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1
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4
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1
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1
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1
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2
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1
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34519
Ito
Purification and characterizat ...
Pseudomonas putida
J. Biochem.
79
1263-1272
1976
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7
4
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1
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2
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1
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1
1
2
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2
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1
1
2
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7
-
4
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1
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1
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1
1
2
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2
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1
1
2
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34527
Ito
Exchange reactions catalyzed b ...
Pseudomonas putida
J. Biochem.
78
1105-1107
1975
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2
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2
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