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deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
DnaG primase and DNA polymerase III holoenzyme are able to bind concurrently to a primed template during DNA replication
-
-
?
Cy3-dATP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
Cy3-dCTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
Cy3-dGTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
Cy3-dUTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
dATP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
dCTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
dGTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I
-
-
?
nucleotide + DNAn
?
-
-
-
-
?
additional information
?
-
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
643657, 643658, 691213, 691785, 692423, 692685, 693797, 703192, 704160, 704278, 705887, 722100 -
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 5'--3' activity
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol III
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol III
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
no exonuclease 5'--3' activity: pol II
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol II
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
can initiate polymer synthesis de novo, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
single strands, pol I, but not pol II and III
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease activity associated with the replicative polymerase is contained within the epsilon subunit
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity, pol I
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 3'--5' activity
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease activity utilizes both, ssDNA and melted dsDNA templates, mismatched basepair is preferred over a correct basepair, removes an incorrect base incorporated opposite a template lesion
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
template specificity of polymerase II
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
nicked duplex is no substrate of pol II and III of E. coli
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
nicked duplex, as poly d(A-T), pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
can not initiate polymer synthesis de novo: pol II and III
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 5'--3' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 5'--3' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
exonuclease 5'--3' activity, pol I
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
physiological role of pol I
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
physiological role of pol I, II and pol III
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
pol III can repair short gaps created by nuclease in duplex DNA, for efficient replication of the long, single-stranded templates pol III requires auxiliary subunits
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
polymerase III: role in replication of chromosomal DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
polymerase II: role in DNA repair
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase V is involved in translesion synthesis and mutagenesis
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
beta sliding clamp plays an essential role in pol V-dependent translesion DNA synthesis in vivo
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase V is involved in translesion synthesis and mutagenesis. Two factors are essential for efficient Pol V-mediated lesion bypass: 1. a DNA substrate onto which the beta-clamp is stably loaded and 2. an extended single-stranded RecA/ATP filament assembled downstream from the lesion site. For efficient bypass, Pol V needs to interact simultaneously with the beta-clamp and the 3' tip of the RecA filament
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
wild-type DinB inserts deoxycytidine opposite N2-furfuryl-dG with 1015fold greater catalytic proficiency than opposite undamaged dG
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the Klenow fragment of DNA polymerase I is able to dimerize on a DNA primer/template. Dimerization is favored when the first molecule is bound in the polymerizing mode, but disfavored when it is bound in the editing mode. Self-association of the polymerase may play an important role in coordinating high-fidelity DNA replication
-
-
?
additional information
?
-
-
Pol beta does incorporate size augmented thymidine analogues besides the unmodified TTP
-
-
?
additional information
?
-
-
small 4'-methyl and -ethyl modifications of the nucleoside triphosphate do not perturb Klenow fragment catalysis
-
-
?
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Kornberg, T.; Kornberg, A.
Bacterial DNA polymerases
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
10
119-144
1974
Bacillus subtilis, Bacteria, Escherichia coli, Micrococcus luteus
-
brenda
Lehman, R.
DNA polymerase I of Escherichia coli
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
14
15-37
1981
Escherichia coli
-
brenda
McHenry, C.; Kornberg, A.
DNA polymerase III holoenzyme
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
14
39-65
1981
Escherichia coli
-
brenda
Setlow, P.
DNA polymerase I from Escherichia coli
Methods Enzymol.
29
3-12
1974
Escherichia coli
brenda
Moses, R.E.
The isolation and properties of DNA polymerase II from Escherichia coli
Methods Enzymol.
29
13-22
1974
Escherichia coli
brenda
Kornberg, T.; Gefter, M.L.
Deoxyribonucleic acid polymerase II (Escherichia coli K12)
Methods Enzymol.
29
22-26
1974
Escherichia coli
brenda
Lehman, I.R.; Bessman, M.J.; Simms, E.S.; Kornberg, A.
Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli
J. Biol. Chem.
233
163-170
1958
Escherichia coli
brenda
Hbscher, U.
DNA polymerase holoenzymes
Trends Biochem. Sci.
9
390-393
1984
Bos taurus, Drosophila melanogaster, Escherichia coli
-
brenda
Beese, L.S.; Friedman, J.M.; Steitz, T.A.
Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate
Biochemistry
32
14095-14101
1993
Escherichia coli (P00582), Escherichia coli
brenda
Murakami-Murofushi, K.; Shioda, M.; Kaji, K.; Yoshida, S.; Murofushi, H.
Inhibition of eukaryotic DNA polymerase alpha with a novel lysophosphatidic acid (PHYLPA) isolated from myxoamoebae of Physarum polycephalum
J. Biol. Chem.
267
21512-21517
1992
Bos taurus, Escherichia coli, Mammalia
brenda
Terry, B.J.; Liu, W.C.; Cianci, C.W.; Proszynski, E.; Fernandes, P.; Bush, K.; Meyers, E.
Inhibition of herpes simplex virus type 1 DNA polymerase by the natural product oosporein
J. Antibiot.
45
286-288
1992
Escherichia coli, Herpes simplex virus, Homo sapiens
brenda
Taguchi, T.; Matsukage, A.; Ito, H.; Saito, Y.; Kawashima, S.
Inhibition of DNA polymerases by tripeptide derivative protease inhibitors
Biochem. Biophys. Res. Commun.
185
1133-1140
1992
Escherichia coli, Rattus norvegicus
brenda
Shioda, M.; Kano, K.; Kobayashi, M.; Kitagawa, I.; Shoji, M.; Yoshida, S.; Ikegami, S.
Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p-hydroquinone sulfate obtained from a marine sponge
FEBS Lett.
350
249-252
1994
Escherichia coli, Mammalia
brenda
Goodman, M.F.; Fygenson, D.K.
DNA polymerase fidelity: from genetics toward a biochemical understanding
Genetics
148
1475-1482
1998
Tequatrovirus T4, Escherichia coli
brenda
Khare, V.; Eckert, K.A.
The proofreading 3'->5' exonuclease activity of DNA polymerases: a kinetic barrier to translesion DNA synthesis
Mutat. Res.
510
45-54
2002
Tequatrovirus T4, Escherichia coli, Herpes simplex virus
brenda
Tuske, S.; Singh, K.; Kaushik, N.; Modak, M.J.
The J-helix of Escherichia coli DNA polymerase I (Klenow fragment) regulates polymerase and 3'-5'-exonuclease functions
J. Biol. Chem.
275
23759-23768
2000
Escherichia coli (P00582)
brenda
Fujii, S.; Gasser, V.; Fuchs, R.P.
The biochemical requirements of DNA polymerase V-mediated translesion synthesis revisited
J. Mol. Biol.
341
405-417
2004
Escherichia coli
brenda
Sutton, M.D.; Duzen, J.M.; Maul, R.W.
Mutant forms of the Escherichia coli beta sliding clamp that distinguish between its roles in replication and DNA polymerase V-dependent translesion DNA synthesis
Mol. Microbiol.
55
1751-1766
2005
Escherichia coli
brenda
Doughty, M.B.; Aboudehen, K.; Anderson, G.; Li, K.; Moore, B.II; Poolson, T.
Side-chain conformational restriction in template-competitive inhibitors of E. coli DNA polymerase I Klenow fragment: synthesis, structural characterization and inhibition activity
Nucleosides Nucleotides Nucleic Acids
23
1751-1765
2004
Escherichia coli
brenda
Bakhtina, M.; Roettger, M.P.; Kumar, S.; Tsai, M.D.
A unified kinetic mechanism applicable to multiple DNA polymerases
Biochemistry
46
5463-5472
2007
African swine fever virus, Escherichia coli, Rattus norvegicus
brenda
Lamers, M.H.; Georgescu, R.E.; Lee, S.G.; ODonnell, M.; Kuriyan, J.
Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III
Cell
126
881-892
2006
Escherichia coli
brenda
Jarosz, D.F.; Godoy, V.G.; Delaney, J.C.; Essigmann, J.M.; Walker, G.C.
A single amino acid governs enhanced activity of DinB DNA polymerases on damaged templates
Nature
439
225-228
2006
Escherichia coli
brenda
Bailey, M.F.; Van der Schans, E.J.; Millar, D.P.
Dimerization of the Klenow fragment of Escherichia coli DNA polymerase I is linked to its mode of DNA binding
Biochemistry
46
8085-8099
2007
Escherichia coli
brenda
Naganuma, M.; Nishida, M.; Kuramochi, K.; Sugawara, F.; Yoshida, H.; Mizushina, Y.
1-deoxyrubralactone, a novel specific inhibitor of families X and Y of eukaryotic DNA polymerases from a fungal strain derived from sea algae
Bioorg. Med. Chem.
16
2939-2944
2008
Tequatrovirus T4, Bos taurus, Brassica oleracea, Escherichia coli, Homo sapiens, Rattus norvegicus, Thermus aquaticus
brenda
Cramer, J.; Rangam, G.; Marx, A.; Restle, T.
Varied active-site constraints in the Klenow fragment of E. coli DNA polymerase I and the lesion-bypass Dbh DNA polymerase
ChemBioChem
9
1243-1250
2008
Escherichia coli
brenda
Nishida, M.; Hada, T.; Kuramochi, K.; Yoshida, H.; Yonezawa, Y.; Kuriyama, I.; Sugawara, F.; Yoshida, H.; Mizushina, Y.
Diallyl sulfides: Selective inhibitors of family X DNA polymerases from garlic (Allium sativum L.)
Food Chem.
108
551-560
2008
Tequatrovirus T4, Bos taurus, Brassica oleracea, Drosophila melanogaster, Escherichia coli, Homo sapiens, Rattus norvegicus, Thermus aquaticus, Oncorhynchus masou
brenda
Takahashi, S.; Yonezawa, Y.; Kubota, A.; Ogawa, N.; Maeda, K.; Koshino, H.; Nakata, T.; Yoshida, H.; Mizushina, Y.
Pyranicin, a non-classical annonaceous acetogenin, is a potent inhibitor of DNA polymerase, topoisomerase and human cancer cell growth
Int. J. Oncol.
32
451-458
2008
Tequatrovirus T4, Bos taurus, Brassica oleracea, Drosophila melanogaster, Escherichia coli, Homo sapiens, Rattus norvegicus, Thermus aquaticus, Oncorhynchus masou
brenda
Kukreti, P.; Singh, K.; Ketkar, A.; Modak, M.J.
Identification of a new motif required for the 3-5 exonuclease activity of Escherichia coli DNA polymerase I (Klenow fragment): the RRRY motif is necessary for the binding of single-stranded DNA substrate and the template strand of the mismatched duplex
J. Biol. Chem.
283
17979-17990
2008
Escherichia coli (P00582), Escherichia coli
brenda
Furukawa, T.; Nishida, M.; Hada, T.; Kuramochi, K.; Sugawara, F.; Kobayashi, S.; Iijima, H.; Shimada, H.; Yoshida, H.; Mizushina, Y.
Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cell growth of Escherichia coli
J. Oleo Sci.
56
43-47
2007
Escherichia coli
brenda
Berdis, A.
Mechanisms of DNA polymerases
Chem. Rev.
109
2862-2879
2009
Geobacillus stearothermophilus, Bacillus subtilis, Tequatrovirus T4, Escherichia phage T7, Escherichia coli, Homo sapiens, Escherichia phage RB69, Thermus aquaticus (P19821)
brenda
Di Pasquale, F.; Fischer, D.; Grohmann, D.; Restle, T.; Geyer, A.; Marx, A.
Opposed steric constraints in human DNA polymerase beta and E. coli DNA polymerase I
J. Am. Chem. Soc.
130
10748-10757
2008
Escherichia coli, Homo sapiens
brenda
Pohlhaus, J.R.; Long, D.T.; OReilly, E.; Kreuzer, K.N.
The epsilon subunit of DNA polymerase III is involved in the nalidixic acid-induced SOS response in Escherichia coli
J. Bacteriol.
190
5239-5247
2008
Escherichia coli, Escherichia coli JH39
brenda
Jiang, Q.; Karata, K.; Woodgate, R.; Cox, M.M.; Goodman, M.F.
The active form of DNA polymerase V is UmuD(2)C-RecA-ATP
Nature
460
359-363
2009
Escherichia coli
brenda
Karata, K.; Vaisman, A.; Goodman, M.F.; Woodgate, R.
Simple and efficient purification of Escherichia coli DNA polymerase V: cofactor requirements for optimal activity and processivity in vitro
DNA Repair
11
431-440
2012
Escherichia coli
brenda
Lin, S.Q.; Bi, L.J.; Zhang, X.E.
A simplified method for reconstituting active E. coli DNA polymerase III
Protein Cell
2
303-307
2011
Escherichia coli
brenda
Walsh, M.T.; Huang, X.
Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy
Nucleic Acids Res.
45
e175
2017
Escherichia coli
brenda
Bogutzki, A.; Naue, N.; Litz, L.; Pich, A.; Curth, U.
E. coli primase and DNA polymerase III holoenzyme are able to bind concurrently to a primed template during DNA replication
Sci. Rep.
9
14460
2019
Escherichia coli
brenda