Cloned (Comment) | Organism |
---|---|
mutants are expressed in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F771A | the mutation shows 30% decreased polymerase activity compared to the wild type enzyme | Escherichia coli |
L823A | the mutation shows 3% decreased polymerase activity compared to the wild type enzyme | Escherichia coli |
R821A | the mutation shows 19% increased polymerase activity compared to the wild type enzyme | Escherichia coli |
R822A | the mutation shows 5% increased polymerase activity compared to the wild type enzyme | Escherichia coli |
R822A/Y824A | the mutation shows 36% increased polymerase activity compared to the wild type enzyme | Escherichia coli |
Y824A | the mutation shows 25% increased polymerase activity compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
deoxynucleoside triphosphate | wild type enzyme, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli | |
0.0015 | - |
deoxynucleoside triphosphate | mutant enzyme R821A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli | |
0.002 | - |
deoxynucleoside triphosphate | mutant enzyme Y824A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli | |
0.0026 | - |
deoxynucleoside triphosphate | mutant enzyme R823A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli | |
0.0034 | - |
deoxynucleoside triphosphate | mutant enzyme R822A/Y824A in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli | |
0.0037 | - |
deoxynucleoside triphosphate | mutant enzyme R822A, in 50 mM Tris-HCl (pH 7.8), 1 mM dithiothreitol, at 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00582 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Escherichia coli | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase I | - |
Escherichia coli |
Klenow fragment | the Klenow fragment of Escherichia coli DNA polymerase I houses catalytic centers for both polymerase and 3'-5' exonuclease activities | Escherichia coli |
Pol I | - |
Escherichia coli |