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Literature summary for 2.7.7.7 extracted from

  • Walsh, M.T.; Huang, X.
    Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy (2017), Nucleic Acids Res., 45, e175 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00022
-
 dGTP pH 8.0, 25°C Escherichia coli
0.00027
-
 dCTP pH 8.0, 25°C Escherichia coli
0.00028
-
 dATP pH 8.0, 25°C Escherichia coli
0.00035
-
 dTTP pH 8.0, 25°C Escherichia coli
0.003
-
 Cy3-dCTP pH 8.0, 25°C Escherichia coli
0.00623
-
 Cy3-dATP pH 8.0, 25°C Escherichia coli
0.00893
-
 Cy3-dUTP pH 8.0, 25°C Escherichia coli
0.0112
-
 Cy3-dGTP pH 8.0, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cy3-dATP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
Cy3-dCTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
Cy3-dGTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
Cy3-dUTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
dATP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
dCTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
dGTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?
dTTP + DNAn systematic determination of the single-turnover incorporation kinetics of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escherichia coli DNA polymerase I Escherichia coli diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymmerase I
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.16
-
 Cy3-dATP pH 8.0, 25°C Escherichia coli
1.57
-
 Cy3-dUTP pH 8.0, 25°C Escherichia coli
1.64
-
 Cy3-dGTP pH 8.0, 25°C Escherichia coli
1.69
-
 dATP pH 8.0, 25°C Escherichia coli
1.75
-
 dCTP pH 8.0, 25°C Escherichia coli
1.75
-
 Cy3-dCTP pH 8.0, 25°C Escherichia coli
6.48
-
 dGTP pH 8.0, 25°C Escherichia coli
6.57
-
 dTTP pH 8.0, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
146
-
 Cy3-dGTP pH 8.0, 25°C Escherichia coli
176
-
 Cy3-dUTP pH 8.0, 25°C Escherichia coli
186
-
 Cy3-dATP pH 8.0, 25°C Escherichia coli
583
-
 Cy3-dCTP pH 8.0, 25°C Escherichia coli
6004
-
 dATP pH 8.0, 25°C Escherichia coli
6480
-
 dCTP pH 8.0, 25°C Escherichia coli
18800
-
 dTTP pH 8.0, 25°C Escherichia coli
29500
-
 dGTP pH 8.0, 25°C Escherichia coli