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Literature summary for 2.7.7.7 extracted from

  • Bakhtina, M.; Roettger, M.P.; Kumar, S.; Tsai, M.D.
    A unified kinetic mechanism applicable to multiple DNA polymerases (2007), Biochemistry, 46, 5463-5472.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzyme R258A are expressed in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R258A | mutant of Pol beta has a facilitated subdomain-reopening step. Rate of single-nucleotide incorporation catalyzed by R258A is identical to that of wild-type Rattus norvegicus

Organism

Organism UniProt Comment Textmining
African swine fever virus
-
-
-
Escherichia coli
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening Escherichia coli diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening African swine fever virus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening. Rotation of the Arg258 side chain is not rate-limiting in the overall kinetic pathway of Pol beta, yet is kinetically significant in subdomain reopening Rattus norvegicus diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase beta
-
 Rattus norvegicus
DNA polymerase I Klenow fragment Escherichia coli
DNA polymerase X
-
 African swine fever virus
Klenow fragment of DNA polymerase I Escherichia coli
Pol X
-
 African swine fever virus