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Literature summary for 2.7.7.7 extracted from

  • Lin, S.Q.; Bi, L.J.; Zhang, X.E.
    A simplified method for reconstituting active E. coli DNA polymerase III (2011), Protein Cell, 2, 303-307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of subunits including alpha, epsilon, theta, gamma, delta', delta, and beta separately or coexpression of the subunits of the pol III core and the clamp loader, as His-tagged proteins in Escherichia coli strain BL21(DE3) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged proteins from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the recombinant subunits including alpha, epsilon, theta, gamma, delta', delta, and beta are purified separately, followed by in vitro reconstitution of the pol III core and clamp loader, or purified by copurification of the subunits of the pol III core and the clamp loader. Pol III core, clamp loader and sliding clamp can be reconstituted effectively to perform DNA polymerization Escherichia coli

Synonyms

Synonyms Comment Organism
DNA polymerase III
-
 Escherichia coli
pol III
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function DNA polymerase III, an enzyme complex consisting of ten subunits, is responsible for genome duplication Escherichia coli