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Literature summary for 2.7.7.7 extracted from

  • Kornberg, T.; Gefter, M.L.
    Deoxyribonucleic acid polymerase II (Escherichia coli K12) (1974), Methods Enzymol., 29, 22-26.
    View publication on PubMed
Search for more literature for 2.7.7.7

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol required for maximal activity Escherichia coli
DTT required for maximal activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Escherichia coli
N-ethylmaleimide abolishes polymerase III activity at 10 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Escherichia coli
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12
-
Escherichia coli
-
 pol III
-

Purification (Commentary)

Purification (Comment) Organism
polymerase III Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 polymerase III Escherichia coli

Storage Stability

Storage Stability Organism
0°C, 3 months, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Escherichia coli diphosphate + DNAn+1
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Escherichia coli