EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
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2.7.7.7 | -999 |
- |
more |
DNA polymerase IV (Dpo4) shows 90-fold higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of extension beyond an 8-oxoG:C pair than an 8-oxoG:A pair. The catalytic efficiency for these events (with dCTP or C) is similar for G and 8-oxoG templates. Extension beyond an 8-oxoG:C pair is similar to G:C and faster than for an 8-oxoG:A pair, in contrast to other polymerases. dCTP insertion opposite 8-oxoG was lower than for opposite guanine |
719836 |
2.7.7.7 | -999 |
- |
more |
DNA synthesis from dNDPs is a little over an order of magnitude lower than from dNTPs and that Vmax/KM is about 400 times lower for dNDPs than for dNTPs |
762278 |
2.7.7.7 | -999 |
- |
more |
kinetic for nucleotide insertion opposite template cytosine |
725350 |
2.7.7.7 | -999 |
- |
more |
kinetic parameters for single dNTP incorporation opposite template 26-mer-N-(deoxyguanosin-8-yl)-1-aminopyrene |
725390 |
2.7.7.7 | -999 |
- |
more |
pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4 |
726028 |
2.7.7.7 | -999 |
- |
more |
pH 7.5, 50°C, kcat/Km is 0.0225/s * mM for incorporation of dTTP in a 61mer template containing N6-furfuryl-deoxyadenosine |
724821 |
2.7.7.7 | -999 |
- |
more |
pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme |
720570 |
2.7.7.7 | -999 |
- |
more |
steady-state kinetic parameters for dCTP incorporation by Dpo4 T239W |
720233 |
2.7.7.7 | -999 |
- |
more |
steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini |
724744 |
2.7.7.7 | -999 |
- |
more |
steady-state kinetic parameters for one-base incorporation |
719838 |