EC Number |
---|
2.7.7.7 | - |
2.7.7.7 | a fusion protein consisting of bacterial maltose-binding protein and full-length HSV 1 POL is constructed with either one factor Xa cleavage site at the linker region or with a second factor Xa site right after Asn-42 of HSV POL. The maltose-binding protein-HSV POL fusion protein is expressed in a baculovirus/insect cell system |
2.7.7.7 | baculovirus-mediated expression in Spodoptera frugiperda (Sf9) cell, human placental alkaline phosphatase signal sequence (MLGPCMLLLLLLLGLRLQLSLG) proves to be optimal for the secretion |
2.7.7.7 | catalytic core of DNA polymerase eta (residues 1-513) |
2.7.7.7 | catalytic subunit of DNA polymerase delta |
2.7.7.7 | catalytic subunits of wild-type and exonuclease-deficient mutant |
2.7.7.7 | DNA polymerase eta phylogenetic analysis, expression of C-terminally His6-tagged enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus expression system |
2.7.7.7 | DNA polymerase gene is expressed under the control of the T7lac promoter on the expression vector pET-28a(+) in Escherichia coli BL21-CodonPlus(DE3)-RIL |
2.7.7.7 | Dpo4 fused to a C-terminal His6 tag is expressed in Escherichia coli strain BL21(DE3) |
2.7.7.7 | expressed in Escherichia coli |