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1-pyrroline-3-hydroxy-5-carboxylate + NAD(P)H
L-hydroxyproline + NAD(P)+
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NADH
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
1-pyrroline-5-carboxylate + NADPH
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
3,4-dehydro-DL-proline + NADPH
?
-
-
-
-
?
3,4-dehydro-L-proline + NAD+
?
activity assay
-
-
?
DELTA1-piperideine-6-carboxylate + NADPH
L-pipecolic acid + NADP+
-
-
-
ir
DELTA1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
r
L-pipecolate + NAD+
DELTA1-piperideine 6-carboxylate + NADH + H+
-
-
-
?
L-pipecolate + NADP+
DELTA1-piperideine 6-carboxylate + NADPH + H+
-
-
-
?
L-Pro + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H
-
the enzyme plays a role as a downstream effector in p53-mediated apoptosis of renal carcinoma cells
-
-
?
L-Pro + NAD+
1-pyrroline-5-carboxylate + NADH
-
-
-
-
?
L-Pro + NADP+
1-pyrroline-5-carboxylate + NADPH
-
-
-
-
?
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD+
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NADP+
1-pyrroline-5-carboxylate + NADPH + H+
L-proline benzyl ester + NAD+
L-pyrroline-5-carboxy benzyl ester + NADH
-
24% of activity with proline
-
-
?
L-proline methyl ester + NAD+
1-pyrroline-5-carboxy methyl ester + NADH
-
16% of activity with proline
-
-
?
L-proline t-butyl ester + NAD+
1-pyrroline-5-carboxy tert-butylester + NADH
-
10% of activity with proline
-
-
?
L-thioproline + NAD(P)+
1-pyrroline-3-thio-5-carboxylate + NAD(P)H
piperideine-6-carboxylate + NADH + H+
L-pipecolic acid + NAD+
-
-
L-pipecolic acid is the sole product
-
ir
piperideine-6-carboxylate + NADPH + H+
L-pipecolic acid + NADP+
-
-
L-pipecolic acid is the sole product
-
ir
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
additional information
?
-
-
not: D-proline, L-hydroxyproline, L-azatidine-2-carboxylate, no other amino acids
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H
L-proline + NAD(P)+
-
-
-
r
1-pyrroline-5-carboxylate + NAD(P)H
L-proline + NAD(P)+
-
-
-
r
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
reversible at pH values above 8 and higher substrate concentrations, oxidation of L-proline is strictly specific for the L-isomer
-
r
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
specific for L-isomer
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
specific for L-isomer
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
reaction is irreversible at pH 7.4, but reversible at pH 10.3
-
-
r
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
specific for L-isomer
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
specific for L-isomer
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
specific for L-isomer
-
-
ir
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
transfers the proS hydrogen at C-4 of the dihydropyridine ring of NAD(P)H to its substrate
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADH + H+
L-proline + NAD+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
activity assay, forward reaction
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
r
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
1-pyrroline-5-carboxylate + NADPH + H+
L-proline + NADP+
-
-
-
?
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H
-
-
-
r
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H
-
-
-
r
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
-
-
-
r
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
-
-
-
r
L-proline + NAD(P)+
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
first step in proline metabolism the hepatocyte nuclear factors HNF4alpha and HNF1alpüha regulate proline metabolism in adult liver
-
-
?
L-proline + NAD+
1-pyrroline-5-carboxylate + NADH + H+
-
-
-
-
r
L-proline + NAD+
1-pyrroline-5-carboxylate + NADH + H+
-
-
-
-
r
L-proline + NADP+
1-pyrroline-5-carboxylate + NADPH + H+
-
-
-
-
r
L-proline + NADP+
1-pyrroline-5-carboxylate + NADPH + H+
-
-
-
-
r
L-thioproline + NAD(P)+
1-pyrroline-3-thio-5-carboxylate + NAD(P)H
-
-
-
r
L-thioproline + NAD(P)+
1-pyrroline-3-thio-5-carboxylate + NAD(P)H
-
-
-
r
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
ping pong mechanism
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
ping pong mechanism
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
transfer of reducing equivalents from host plants to symbiotic partner
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
third enzyme in proline pathway
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
linkage to hexose monophosphate pathway
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
final reaction of proline synthesis
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
-
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
transfer of reducing equivalents into mitochondria, linkage to pentose phosphate pathway
-
-
?
pyrroline-5-carboxylate + NAD(P)H + H+
L-proline + NAD(P)+
-
discussion of role in vivo
-
-
?
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([[(3,5-dibromophenyl)methyl]amino]methylene)bis(phosphonic acid)
competitive inhibition against both 1-pyrroline 5-carboxylate and NADPH. Growth of myelogenous erythroleukemic K562 and epithelial breast cancer MDA-MB-231 cell lines is progressively impaired by concentrations ranging from 1-100 microM
-
([[(3,5-dichlorophenyl)methyl]amino]methylene)bis(phosphonic acid)
competitive inhibition against both 1-pyrroline 5-carboxylate and NADPH
-
([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid)
-
71.2% inhibition at 0.2 mM
2,3-dichlorophenylhydroxymethylenebisphosphonic acid
-
-
2-(2,3-dichlorophenyl)-1-hydroxyethylidenebisphosphonic acid
-
-
2-(2,3-dichlorophenylamino)ethylidenebisphosphonic acid
-
-
2-(2,6-dichlorophenyl)-1-hydroxyethylidenebisphosphonic acid
-
-
2-(2,6-dichlorophenylamino)ethylidenebisphosphonic acid
-
-
2-(3,5-di(trifluoromethyl)phenylamino)ethylidenebisphosphonic acid
-
-
2-(3,5-dichlorophenylamino)ethylidenebisphosphonic acid
-
-
2-(3,5-dimethylphenyl)-1-hydroxyethylidenebisphosphonic acid
-
-
2-(3,5-dimethylphenylamino)ethylidenebisphosphonic acid
-
-
2-(4-benzylphenylamino)ethylidenebisphosphonic acid
-
-
2-(4-chlorophenyl)-1-hydroxyethylidenebisphosphonic acid
-
-
3,5-di(trifluoromethyl)phenylaminomethylenebisphosphonic acid
-
-
3,5-dibromophenylaminomethylenebisphosphonic acid
-
-
3,5-difluorophenylaminomethylenebisphosphonic acid
-
-
3,5-dimethylphenylhydroxymethylenebisphosphonic acid
-
-
3-acetylpyridine analogue of NAD+
-
95% inhibition at 2 mM
3-carbamoylphenylaminomethylenebisphosphonic acid
-
-
3-chlorophenylaminomethylenebisphosphonic acid
-
-
4-benzylphenylaminomethylenebisphosphonic acid
-
-
4-benzylphenylhydroxymethylenebisphosphonic acid
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
90% inhibition at 0.012 mM
5,6,7,8-tetrahydro-2-naphthylaminomethylenebisphosphonic acid
-
-
Cl-
progressive stimulation from 20-200 mM, inhibitory above
CTP
-
91% inactivation at 1 mM reversible with Mg2+
cyclopentanecarboxylate
-
cysteine
-
77% inactivation at 10 mM
D-allohydroxyproline
-
competitive inhibition
DL-pipecolic acid
-
65% inhibition at 10mM
GTP
-
100% inactivation at 10 mM, reversible with Mg2+
indan-5-ylaminomethylenebisphosphonic acid
-
-
iodoacetamide
-
complete inactivation at 1 mM
iodoacetate
-
10% inactivation at 1 mM
L-2-acetidine-4-carboxylic acid
-
50% inactivation at 2 mM
L-hydroxyproline
-
competitive inhibition
L-tetrahydro-2-furoic acid
-
L-thiazolidine-2-carboxylate
-
-
L-thiazolidine-4-carboxylate
-
N-ethylmaleimide
-
90% inhibition at 0.02 mM, inhibition can be prevented by NADH
N-formyl-L-proline
competitive. Compound phenocopies the PYCR1 knockdown in MCF10A H-RASV12 breast cancer cells by inhibiting de novo proline biosynthesis and impairing spheroidal growth
-
NaHSO3
-
88% inactivation at 1 mM
p-hydroxymercuribenzoate
-
90% inhibition at 0.008 mM, inhibition can be prevented by addition of excess dithiothreitol
stearoyl-CoA
-
competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity
thiazolidine-4-carboxylic acid
thio-NAD+
-
65% inactivation at 0.2 mM
thio-NADP+
-
65% inactivation at 0.2 mM
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
[[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
52% inhibition at 0.2 mM
[[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
63.9% inhibition at 0.2 mM
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
[[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
92.5% inhibition at 0.2 mM
[[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid)
-
98.4% inhibition at 0.2 mM
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
[[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
71.2% inhibition at 0.2 mM
[[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid)
-
94.5% inhibition at 0.2 mM
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
ADP
-
-
Ag+
-
-
Ag+
-
65% inactivation at 0.3 mM
Ag+
-
44% inactivation at 0.7 mM
Ag+
-
inactivation at 0.1 mM
ATP
-
70% inhibition at 2 mM
ATP
-
competitive with respect to NADH, 50% inhibition at 0.1 mM
ATP
-
41% inactivation at 1 mM
ATP
-
reversible with Mg2+
Cd2+
-
90% inhibition at 3.3 mM
Cu2+
-
90% inhibition at 3.3 mM
Hg2+
-
-
Hg2+
-
65% inactivation at 0.3 mM
Hg2+
-
75% inactivation at 0.1 mM
KCl
inhibitory on NADH-dependent reaction
KCl
-
68% inhibition at 2 M
L-proline
-
94% inhibition at 10 mM
L-proline
at 0-500 microM, the value of KMapp increases whereas Vlim remains fairly unchanged, which is consistent with competitive inhibition
L-proline
-
L-proline inhibits the inverse reaction at a concentration of 10-20 mM
L-proline
-
L-proline inhibits the inverse reaction at a concentration of 10-20 mM
NaCl
inhibitory on NADH-dependent reaction
NaCl
-
70% inhibition at 2 M
NAD+
-
-
NADH
-
substrate inhibition
NADH
-
substrate inhibition
NADH
-
slight inhibition with 25% reduction of the catalytic rate at 200 mM
NADP+
-
NADP+
-
43% inactivation at 1 mM
NADPH
-
above 0.13 mM
NADPH
substrate inhibition
NH2OH
-
92% inactivation at 1 mM
NH2OH
-
7% inactivation at 1 mM
p-chloromercuribenzoate
-
not
p-chloromercuribenzoate
-
not
p-chloromercuribenzoate
-
complete inhibition at 0.125 mM, can be prevented by addition of dithiothreitol
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
complete inactivation at 1 mM
p-chloromercuribenzoate
-
-
proline
-
39% inhibition of the NADH dependent activity and 17% of the NADPH dependent activity at 10 mM
proline
-
competitive inhibition
proline
-
89% inhibition at 2 M
proline
-
12% inactivation at 50 mM
proline
-
competitive inhibition
proline
-
competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity
proline
-
75% inactivation at 50 mM
pyrroline-5-carboxylate
-
substrate inhibition
pyrroline-5-carboxylate
-
substrate inhibition
thiazolidine-4-carboxylic acid
-
-
thiazolidine-4-carboxylic acid
-
42% inhibition at 10 mM, L-isomer
thiazolidine-4-carboxylic acid
-
-
Zn2+
-
-
Zn2+
-
14% inactivation at 1 mM
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
-
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
95.4% inhibition at 0.2 mM
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
-
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
96.2% inhibition at 0.2 mM
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
-
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
91.1% inhibition at 0.2 mM
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
-
-
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
-
70.5% inhibition at 0.2 mM
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
-
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
-
61.5% inhibition at 0.2 mM
additional information
not inhibitory: NAD+
-
additional information
-
not inhibitory: NAD+
-
additional information
-
-
-
additional information
-
enzyme is not inhibited by the proline-analog L-thiazolidine-4-carboxylic acid
-
additional information
-
not inhibited by NADPH
-
additional information
-
enzyme is not inhibited by the proline-analog L-thiazolidine-4-carboxylic acid
-
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0.02 - 2.887
1-pyrroline-5-carboxylate
0.141
3,4-dehydro-L-proline
-
4.4
DELTA1-pyrroline-3-hydroxy-5-carboxylate
-
-
0.051 - 0.62
DL-pyrroline-5-carboxylate
0.33
L-pyrroline-5-carboxylate
additional information
additional information
-
0.02
1-pyrroline-5-carboxylate
cosubstrate NADPH, pH 7.5, 35°C
0.022
1-pyrroline-5-carboxylate
-
cosubstrate NADPH
0.024 - 0.025
1-pyrroline-5-carboxylate
-
-
0.0277
1-pyrroline-5-carboxylate
pH 7, 28°C
0.04
1-pyrroline-5-carboxylate
-
cofactor NADH
0.047
1-pyrroline-5-carboxylate
-
cofactor NADPH
0.09
1-pyrroline-5-carboxylate
-
-
0.09
1-pyrroline-5-carboxylate
-
with NADH
0.096
1-pyrroline-5-carboxylate
cofactor NADPH, pH 7.8, 35°C
0.12
1-pyrroline-5-carboxylate
-
with NADPH
0.12
1-pyrroline-5-carboxylate
-
with NADPH
0.125
1-pyrroline-5-carboxylate
-
-
0.14
1-pyrroline-5-carboxylate
-
with NADH
0.15
1-pyrroline-5-carboxylate
-
with NADPH
0.154
1-pyrroline-5-carboxylate
-
-
0.17
1-pyrroline-5-carboxylate
-
-
0.17
1-pyrroline-5-carboxylate
-
-
0.184
1-pyrroline-5-carboxylate
-
apparent value, with NADPH as cosubstrate, at pH 7.5 and 37°C
0.19
1-pyrroline-5-carboxylate
-
-
0.2
1-pyrroline-5-carboxylate
-
with NADH
0.21
1-pyrroline-5-carboxylate
-
cosubstrate NADH
0.24
1-pyrroline-5-carboxylate
cosubstrate NADH, pH 7.5, 35°C
0.273
1-pyrroline-5-carboxylate
cosubstrate NADH, pH 7.5, 30°C
0.317
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant mutant R119C, with NADH
0.32
1-pyrroline-5-carboxylate
mutant R119C, cosubstrate NADH, pH 7.5, 37°C
0.33
1-pyrroline-5-carboxylate
mutant R119C, cosubstrate NADPH, pH 7.5, 37°C
0.334
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant mutant R119C, with NADPH
0.349
1-pyrroline-5-carboxylate
-
apparent value, with NADH as cosubstrate, at pH 7.5 and 37°C
0.37
1-pyrroline-5-carboxylate
-
with NADPH
0.44
1-pyrroline-5-carboxylate
-
-
0.45
1-pyrroline-5-carboxylate
-
with NADPH
0.53
1-pyrroline-5-carboxylate
-
with NADH
0.667
1-pyrroline-5-carboxylate
cosubstrate NADPH, wild-type, pH 7.5, 37°C
0.915
1-pyrroline-5-carboxylate
cofactor NADH, pH 7.8, 35°C
0.949
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant wild-type enzyme, with NADPH
0.99
1-pyrroline-5-carboxylate
wild-type, cosubstrate NADPH, pH 7.5, 37°C
1.053
1-pyrroline-5-carboxylate
cosubstrate NADPH, pH 7.5, 30°C
1.315
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant mutant R251C, with NADH
1.32
1-pyrroline-5-carboxylate
mutant R251C, cosubstrate NADH, pH 7.5, 37°C
1.499
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant mutant R251C, with NADPH
1.5
1-pyrroline-5-carboxylate
mutant R251C, cosubstrate NADPH, pH 7.5, 37°C
1.509
1-pyrroline-5-carboxylate
pH 7.5, 37°C, recombinant wild-type enzyme, with NADH
1.51
1-pyrroline-5-carboxylate
wild-type, cosubstrate NADH, pH 7.5, 37°C
1.62 - 1.64
1-pyrroline-5-carboxylate
-
-
2.887
1-pyrroline-5-carboxylate
mutant T238A, wild-type, pH 7.5, 37°C
0.051
DL-pyrroline-5-carboxylate
-
cosubstrate NADPH
0.08
DL-pyrroline-5-carboxylate
-
-
0.34
DL-pyrroline-5-carboxylate
-
-
0.62
DL-pyrroline-5-carboxylate
-
-
0.62
DL-pyrroline-5-carboxylate
-
cosubstrate NADH
0.12
L-proline
-
-
1.55
L-proline
-
L-proline oxidation
0.33
L-pyrroline-5-carboxylate
-
pH 8.0
0.33
L-pyrroline-5-carboxylate
-
pH 6.5
0.151
NAD+
-
wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay
0.235
NAD+
-
E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay
10.5
NAD+
-
L-proline oxidation
0.025
NADH
-
-
0.03
NADH
mutant R119C, pH 7.5, 37°C
0.034
NADH
pH 7.5, 37°C, recombinant mutant R119C, with 1-pyrroline-5-carboxylate
0.07
NADH
wild-type, pH 7.5, 37°C
0.176
NADH
-
apparent value, at pH 7.5 and 37°C
0.298
NADH
pH 7.5, 37°C, recombinant wild-type enzyme, with 1-pyrroline-5-carboxylate
0.3
NADH
wild-type, pH 7.5, 37°C
0.43
NADH
-
etiolated shoots
0.95
NADH
mutant R251C, pH 7.5, 37°C
0.953
NADH
pH 7.5, 37°C, recombinant mutant R251C, with 1-pyrroline-5-carboxylate
0.093
NADP+
-
-
0.48
NADP+
-
E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay
0.73
NADP+
-
E221A mutant, fixed 1 mM NADP+
1.26
NADP+
-
wild type enzyme, fixed 1 mM NADP+
3.06
NADP+
-
wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay
0.006
NADPH
-
-
0.012
NADPH
-
apparent value, at pH 7.5 and 37°C
0.1
NADPH
-
etiolated shoots
0.119
NADPH
pH 7.5, 37°C, recombinant mutant R251C, with 1-pyrroline-5-carboxylate
0.12
NADPH
mutant R251C, pH 7.5, 37°C
0.159
NADPH
mutant T238A, pH 7.5, 37°C
0.216
NADPH
pH 7.5, 37°C, recombinant wild-type enzyme, with 1-pyrroline-5-carboxylate
0.22
NADPH
wild-type, pH 7.5, 37°C
0.283
NADPH
wild-type, pH 7.5, 37°C
0.537
NADPH
pH 7.5, 37°C, recombinant mutant R119C, with 1-pyrroline-5-carboxylate
0.54
NADPH
mutant R119C, pH 7.5, 37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
steady-state kinetics of the wild-type enzyme and mutants
-
additional information
additional information
-
steady-state kinetics of the wild-type enzyme and mutants
-
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0.00039 - 0.00048
([[(3,5-dibromophenyl)methyl]amino]methylene)bis(phosphonic acid)
-
0.00074 - 0.00078
([[(3,5-dichlorophenyl)methyl]amino]methylene)bis(phosphonic acid)
-
0.00078
([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
1.187
2,3-dichlorophenylhydroxymethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.488
2-(2,3-dichlorophenyl)-1-hydroxyethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.431
2-(2,3-dichlorophenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
1.639
2-(2,6-dichlorophenyl)-1-hydroxyethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.763
2-(2,6-dichlorophenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.463
2-(3,5-di(trifluoromethyl)phenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.082
2-(3,5-dichlorophenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
1.914
2-(3,5-dimethylphenyl)-1-hydroxyethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.386
2-(3,5-dimethylphenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.488
2-(4-benzylphenylamino)ethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
2.071
2-(4-chlorophenyl)-1-hydroxyethylidenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.413
3,5-di(trifluoromethyl)phenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.054
3,5-dibromophenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.719
3,5-difluorophenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
1.409
3,5-dimethylphenylhydroxymethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.547
3-carbamoylphenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
1.618
3-chlorophenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.852
4-benzylphenylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.325
4-benzylphenylhydroxymethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.0087 - 0.234
4-bromopargyline
-
0.684
5,6,7,8-tetrahydro-2-naphthylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
1.19
cyclopentanecarboxylate
Homo sapiens
pH 7.8, temperature not specified in the publication
29
EDTA
Streptococcus pyogenes
-
at pH 7.5 and 37°C
1.887
indan-5-ylaminomethylenebisphosphonic acid
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
200
KCl
Arabidopsis thaliana
cofactor NADH, pH 7.8, 35°C
2.25
L-tetrahydro-2-furoic acid
Homo sapiens
pH 7.8, temperature not specified in the publication
0.44
L-thiazolidine-2-carboxylate
Homo sapiens
pH 7.8, temperature not specified in the publication
-
0.6
L-thiazolidine-4-carboxylate
Homo sapiens
pH 7.8, temperature not specified in the publication
0.1
N-formyl-L-proline
Homo sapiens
pH 7.8, temperature not specified in the publication
-
0.045
NADPH
Trypanosoma cruzi
pH 7, 28°C
0.00087 - 0.335
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.012
[[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.0027
[[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.00088 - 0.45
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.00039
[[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.00022
[[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.00022 - 0.134
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.0256 - 1.245
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
0.0009
[[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.00041
[[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.0014 - 0.97
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.00039
([[(3,5-dibromophenyl)methyl]amino]methylene)bis(phosphonic acid)
Homo sapiens
substrate NADPH, pH 7.5, 30°C
-
0.00048
([[(3,5-dibromophenyl)methyl]amino]methylene)bis(phosphonic acid)
Homo sapiens
substrate NADH, pH 7.5, 30°C
-
0.00074
([[(3,5-dichlorophenyl)methyl]amino]methylene)bis(phosphonic acid)
Homo sapiens
substrate NADPH, pH 7.5, 30°C
-
0.00078
([[(3,5-dichlorophenyl)methyl]amino]methylene)bis(phosphonic acid)
Homo sapiens
substrate NADH, pH 7.5, 30°C
-
0.0087
4-bromopargyline
Homo sapiens
substrate NADPH, pH 7.5, 30°C
-
0.234
4-bromopargyline
Homo sapiens
substrate NADH, pH 7.5, 30°C
-
0.485
Cl-
Medicago truncatula
cofactor NADH, pH 7.5, 35°C
0.704
Cl-
Medicago truncatula
cofactor NADPH, pH 7.5, 35°C
1.72
L-proline
Homo sapiens
pH 7.8, temperature not specified in the publication
96
L-proline
Zymomonas mobilis subsp. mobilis
substrate NADH, pH 7.5, 30°C
107
L-proline
Zymomonas mobilis subsp. mobilis
substrate NADPH, pH 7.5, 30°C
130
NaCl
Zymomonas mobilis subsp. mobilis
substrate NADPH, pH 7.5, 30°C
200
NaCl
Arabidopsis thaliana
cofactor NADH, pH 7.8, 35°C
1144
NaCl
Zymomonas mobilis subsp. mobilis
substrate NADH, pH 7.5, 30°C
1.4
NAD+
Zymomonas mobilis subsp. mobilis
substrate NADPH, pH 7.5, 30°C
7.4
NAD+
Zymomonas mobilis subsp. mobilis
substrate NADH, pH 7.5, 30°C
0.032
NADP+
Arabidopsis thaliana
cofactor NADH, pH 7.8, 35°C
1.44
NADP+
Arabidopsis thaliana
cofactor NADPH, pH 7.8, 35°C
22.6
NADP+
Zymomonas mobilis subsp. mobilis
substrate NADH, pH 7.5, 30°C
0.081
Pargyline
Homo sapiens
substrate NADPH, pH 7.5, 30°C
10
Pargyline
Homo sapiens
IC50 value above 10 mM, substrate NADH, pH 7.5, 30°C
0.00087
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.335
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.00088
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.45
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.00022
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.134
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.0256
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
1.245
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
0.0014
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
Streptococcus pyogenes
-
pH 7.5-8.0, at 37°C
0.97
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
Arabidopsis thaliana
-
in 100 mM HEPES-KOH buffer, pH 7.75, at 35°C
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Hill, D.L.; Chambers, P.
The biosynthesis of proline by Tetrahymena pyriformis
Biochim. Biophys. Acta
148
435-447
1967
Tetrahymena pyriformis
brenda
Krishna, R.V.; Beilstein, P.; Leisinger, T.
Biosynthesis of proline in Pseudomonas aeruginosa. Properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase
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