Cloned (Comment) | Organism |
---|---|
cloned into the pET28a+ vector with a His6 tag at the N-terminus for expression in the Escherichia coli strain B834DE3 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
2.8 A resolution structure of the pyrroline-5-carboxylate reductase apo enzyme, and its ternary complex with NADPH and substrate analog at 3.1 A | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E221A | affinity for substrates is increased | Homo sapiens |
E221G | insoluble protein | Homo sapiens |
additional information | N-truncation results in an insoluble protein, C-truncation does not alter the activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
proline | competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity | Homo sapiens | |
stearoyl-CoA | competitive inhibitor of P5CR NAD(P)H-dependent thioproline dehydrogenase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.151 | - |
NAD+ | wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay | Homo sapiens | |
0.235 | - |
NAD+ | E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay | Homo sapiens | |
0.48 | - |
NADP+ | E221A mutant, 1 mM fixed thioproline, thioproline dehydrogenase activity assay | Homo sapiens | |
0.73 | - |
NADP+ | E221A mutant, fixed 1 mM NADP+ | Homo sapiens | |
1.26 | - |
NADP+ | wild type enzyme, fixed 1 mM NADP+ | Homo sapiens | |
3.06 | - |
NADP+ | wild type enzyme, 1 mM fixed thioproline, thioproline dehydrogenase activity assay | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + NAD(P)+ | Homo sapiens | - |
1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA agarose, a Superdex200 and a Resource Q column | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
refolding after treatment with urea | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + NAD(P)+ | - |
Homo sapiens | 1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | five homodimer subunits | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
P5CR | - |
Homo sapiens |
pyrroline-5-carboxylate reductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
NADP+ | E221A mutant, fixed 1 mM NADP+ | Homo sapiens | |
55 | - |
NADP+ | wild type enzyme, fixed 1 mM NADP+ | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Homo sapiens |
10 | - |
L-proline dehydrogenase activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |