Any feedback?
Literature summary for 1.5.1.2 extracted from
- Purification to homogeneity of pyrroline-5-carboxylate reductase of barley (1986), Plant Physiol., 80, 142-144.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
up to 480000 kDa, quarternary structure changes drastically with buffer environment | Hordeum vulgare |
| 30000 | - |
- |
Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 507 | - |
- |
Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 5% glycerol, 2 mM dithiothreitol, 1 month | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Hordeum vulgare | L-proline + NAD(P)+ | - |
? |  |
| L-proline benzyl ester + NAD+ | 24% of activity with proline | Hordeum vulgare | L-pyrroline-5-carboxy benzyl ester + NADH | - |
? | |
| L-proline methyl ester + NAD+ | 16% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy methyl ester + NADH | - |
? | |
| L-proline t-butyl ester + NAD+ | 10% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy tert-butylester + NADH | - |
? | |
| additional information | not: D-proline, L-hydroxyproline, L-azatidine-2-carboxylate, no other amino acids | Hordeum vulgare | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| polymer | 12-16 * 30000 SDS-PAGE | Hordeum vulgare |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1270 | - |
pyrroline-5-carboxylate | - |
Hordeum vulgare | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | pyrroline-5-carboxylate reductase activity | Hordeum vulgare |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
no proline dehydrogenase activity below pH 8.5 | Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Hordeum vulgare | |
| NADPH | - |
Hordeum vulgare | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
