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Literature summary for 1.5.1.2 extracted from

  • Krishna, R.V.; Beilstein, P.; Leisinger, T.
    Biosynthesis of proline in Pseudomonas aeruginosa. Properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase (1979), Biochem. J., 181, 223-230.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

Inhibitors

Inhibitors Comment Organism Structure
3-acetylpyridine analogue of NAD+ 95% inhibition at 2 mM Pseudomonas aeruginosa
Ag+ inactivation at 0.1 mM Pseudomonas aeruginosa
Hg2+ 75% inactivation at 0.1 mM Pseudomonas aeruginosa
iodoacetamide complete inactivation at 1 mM Pseudomonas aeruginosa
L-2-acetidine-4-carboxylic acid 50% inactivation at 2 mM Pseudomonas aeruginosa
p-chloromercuribenzoate complete inactivation at 1 mM Pseudomonas aeruginosa
proline 75% inactivation at 50 mM Pseudomonas aeruginosa
thio-NAD+ 65% inactivation at 0.2 mM Pseudomonas aeruginosa
thio-NADP+ 65% inactivation at 0.2 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADPH
-
 Pseudomonas aeruginosa
0.05
-
 NADH
-
 Pseudomonas aeruginosa
0.09
-
 1-pyrroline-5-carboxylate with NADH Pseudomonas aeruginosa
0.12
-
 1-pyrroline-5-carboxylate with NADPH Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 sucrose density gradient sedimentation, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyrroline-5-carboxylate + NAD(P)H + H+ Pseudomonas aeruginosa
-
 L-proline + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas aeruginosa
3317
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+ specific for L-isomer Pseudomonas aeruginosa L-proline + NAD(P)+
-
 ir
1-pyrroline-5-carboxylate + NADH + H+
-
 Pseudomonas aeruginosa L-proline + NAD+
-
 r
1-pyrroline-5-carboxylate + NADPH + H+
-
 Pseudomonas aeruginosa L-proline + NADP+
-
 r
pyrroline-5-carboxylate + NAD(P)H + H+
-
 Pseudomonas aeruginosa L-proline + NAD(P)+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.5
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NADH preferentially Pseudomonas aeruginosa
NADPH
-
 Pseudomonas aeruginosa