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Literature summary for 1.5.1.2 extracted from

  • Kenklies, J.; Ziehn, R.; Fritsche, K.; Pich, A.; Andreesen, J.R.
    Proline biosynthesis from L-ornithine in Clostridium sticklandii: purification of DELTA1-pyrroline-5-carboxylate reductase, and sequence and expression of the encoding gene, proC (1999), Microbiology, 145, 819-826.
No PubMed abstract available
Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Acetoanaerobium sticklandii

Inhibitors

Inhibitors Comment Organism Structure
ATP 70% inhibition at 2 mM Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 NADH
-
 Acetoanaerobium sticklandii
0.19
-
 1-pyrroline-5-carboxylate
-
 Acetoanaerobium sticklandii
1.55
-
 L-proline L-proline oxidation Acetoanaerobium sticklandii
10.5
-
 NAD+ L-proline oxidation Acetoanaerobium sticklandii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Acetoanaerobium sticklandii
potassium acetate increase at 3 mM Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 8 * 25000 SDS-PAGE Acetoanaerobium sticklandii
230000
-
 gel filtration Acetoanaerobium sticklandii

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
173.4
-
-
 Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+ reversible at pH values above 8 and higher substrate concentrations, oxidation of L-proline is strictly specific for the L-isomer Acetoanaerobium sticklandii L-proline + NAD(P)+
-
 r
1-pyrroline-5-carboxylate + NADH + H+
-
 Acetoanaerobium sticklandii L-proline + NAD+
-
 r
L-proline + NAD+
-
 Acetoanaerobium sticklandii 1-pyrroline-5-carboxylate + NADH + H+
-
 r

Subunits

Subunits Comment Organism
octamer 8 * 25000 SDS-PAGE Acetoanaerobium sticklandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Acetoanaerobium sticklandii
10
-
 L-proline oxidation Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
NADH preferentially Acetoanaerobium sticklandii
NADH L-proline oxidation is strictly NAD+ dependent Acetoanaerobium sticklandii
NADPH
-
 Acetoanaerobium sticklandii