Advanced Search

Search Application

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term: diagnostics

Results 1 - 100 of 424 > >>
EC Number
Application
Commentary
aldehyde reductase
diagnostics
AKR1B10 may have a potential role as a tumor marker
L-lactate dehydrogenase
diagnostics
the enzyme is useful as marker for endometrial carcinoma
D-lactate dehydrogenase
diagnostics
the enzyme is useful for selective determination of D,L-lactic acid in wines using peroxidase-based biosensors, method optimization, overview
3-hydroxybutyrate dehydrogenase
diagnostics
the enzyme is a useful marker in the assay of diabetes mellitus and/or ketoacidosis
isocitrate dehydrogenase (NADP+)
diagnostics
IDH1 codon 132 mutation is an important prognostic biomarker in gliomas. IDH1 mutation predicts outcome in grade 2, 3, and 4 gliomas
isocitrate dehydrogenase (NADP+)
diagnostics
IDH1 mutational analysis can serve as a useful diagnostic marker of a glioma
glucose 1-dehydrogenase [NAD(P)+]
diagnostics
usage for quantitative determination of glucose in clinical tests and in the food industry
glucose 1-dehydrogenase [NAD(P)+]
diagnostics
enzyme can be used for glucose determination
isopropanol dehydrogenase (NADP+)
diagnostics
characterization of adrenal and gonadal tissue
carnitine 3-dehydrogenase
diagnostics
the L-carnitine oxidation step can be exploited for spectroscopic L-carnitine determination in biological fluids
carbonyl reductase (NADPH)
diagnostics
expression of CBR mRNA is a significant prognostic factor in non–small-cell lung cancer and is inversely associated with tumor progression and angiogenesis
3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
diagnostics
co-immobilization of the enzyme with diaphorase of Clostridum sp. onto alkylamine glass beads through glutaraldehyde coupling for determination of bile acids in serum and bile in a cost-effective colorimetric assay
mannitol dehydrogenase
diagnostics
the enzyme is a major food allergen and its occurance in food, e.g. in celery sticks, celeriac, celery powder, or celery seeds, is required to be labeled, development of a quantitative PCR-based DNA detection method for use in food samples, overview
S-(hydroxymethyl)glutathione dehydrogenase
diagnostics
the enzyme is useful for determination of formaldehyde in consumers goods and environment
glucose oxidase
diagnostics
glucose oxidase can be used in various immunoassays and/or staining procedures as well as removal of excess glucose, in real-time fluorescent microscopy for biological samples, glucose oxidase/catalase is often used for oxygen scavenging to reduce photodamage
glucose oxidase
diagnostics
used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine
cholesterol oxidase
diagnostics
the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
cholesterol oxidase
diagnostics
the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
cholesterol oxidase
diagnostics
analytic tool for determining cholesterol in various samples
galactose oxidase
diagnostics
determination of galactose or lactose concentration in complex biological fluids by immobilized galactose oxidase
(S)-2-hydroxy-acid oxidase
diagnostics
lactate biosensor development based on an enzymatic recognition system using lactate oxidase and a transduction system based on laminol, peroxidase from Arthromyces ramosus and metallic aluminum, immobilized in a plastic support by a polyion complex membrane prepared from poly-L-lysine hydrobromide and poly(sodium 4-styrenesulfonate)
(S)-2-hydroxy-acid oxidase
diagnostics
lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
glucose 1-dehydrogenase (FAD, quinone)
diagnostics
glucose sensor/glucose determination
glucose 1-dehydrogenase (FAD, quinone)
diagnostics
the enzyme can be used as O2-independent biosensor for glucoe detection
6-oxohexanoate dehydrogenase
diagnostics
development of a biosensor for detection of caprolactam oligomers in aqueous media
trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
diagnostics
overexpression of dihydrodiol dehydrogenase as a prognostic marker in resected gastric cancer patients, patients with DDH overexpression have a significantly higher incidence of early tumor recurrence and distant metastasis
7-dehydrocholesterol reductase
diagnostics
enzyme activity measurement can be a tool for prognosis of the Smith-Lemli-Opitz syndrome
7-dehydrocholesterol reductase
diagnostics
enzyme is a key marker of early Leydig cell steroidogenesis, using the technique of differential display RT-PCR
7-dehydrocholesterol reductase
diagnostics
enzyme activity measurement is useful for distinguishing Smith-Lemli-Opitz syndrome from carrier or unaffected cells, diagnosis of atypical cases, overview
protoporphyrinogen oxidase
diagnostics
detection of the naturally occuring 1082-1083insC mutation in the PPOX gene is a genetic marker for variegate porphyria in humans
bilirubin oxidase
diagnostics
the BOD activity catalyzing the oxidation of bilirubin to biliverdin can be used for the diagnosis of jaundice and hyperbilirubinemia
bilirubin oxidase
diagnostics
BOD is used for diagnostic analysis of bilirubin in serum
succinate dehydrogenase
diagnostics
succinate dehydrogenase subunits may be candidate susceptibility genes for some phosphatase and tensin homolog (PTEN, tumor suppressor) mutation-negative individuals with Cowden syndrome-like cancers. To address this hypothesis, germline succinate dehydrogenase subunits SDHB-D mutation analysis in 375 PTEN mutation-negative Cowden syndrome /Cowden syndrome -like individuals is performed, followed by functional analysis of identified SDH mutations/variants. Germline SDH mutations/variants occur in a subset of PTEN mutation-negative Cowden syndrome /Cowden syndrome -like individuals and are associated with increased frequencies of breast, thyroid, and renal cancers beyond those conferred by germline PTEN mutations. SDH testing should be considered for germline PTEN mutation-negative Cowden syndrome /Cowden syndrome -like -like individuals, especially in the setting of breast, thyroid, and/or renal cancers
succinate dehydrogenase
diagnostics
SDHB mutations are a marker for a prognostic risk of patients with pheochromocytomas or paragangliomas
succinate dehydrogenase
diagnostics
mutations of SDH subunit encoding genes are good markers for paraganglioma
isovaleryl-CoA dehydrogenase
diagnostics
enzyme activity assay development for determination of isovaleric acidemia in newborn disorder screening
glutamate dehydrogenase
diagnostics
GLDH, gama-glutamyltransferase, aspartate-aminotransferase, alanine-aminotransferase and erythrocyte mean cell volume are assessed in 238 alcoholics admitted to hospital: on admission, after 24 h and after 7 days. All the values are significantly higher than those in healthy persons. The fastest activity decrease is seen in GLDH. The kinetics of GLDH and aspartate-aminotranferase are more applicable than gama-glutamyltransferase kinetics after a week, but GLDH kinetics are most reliable. GLDH is the most specific laboratory marker with almost 90% specificity. The sensitivity of combination erythrocyte mean cell volume and GLDH kinetics after 1 week of abstinence is pathognomonic by 97.2%. GLDH is an equally accurate marker of alcoholism in comparison to others
glutamate dehydrogenase
diagnostics
GDH electrophoretic type (ETs) and sequence types may serve as useful markers in predicting the pathogenic behavior of strains of this serotype and that the molecular basis for the observed differences in the ETs is amino acid substitutions and not deletion, insertion, or processing uniqueness
phenylalanine dehydrogenase
diagnostics
the immobilized enzyme is useful for establishing an analytical spectrophotometric determination method of L-Phe in the diagnostics of phenyketonuria, an inborn error of amino acid metabolism in which the conversion of L-phenylalanine to L-tyrosine is impaired and can cause profound mental retardation if not detected and treated soon after birth. Early quantitative measurement of the plasma L-Phe is essential for the diagnosis of phenylketonuria and the control of dietary therapy of the patients. The conversion efficiency of the reactor with immobillized enzyme is 100% in the range of 0.005-0.6 mM Phe at 9 mM NAD+ with a total flow rate of 0.1 mL/min. The reactor is used for the analyses of 30 samples each for 3 h per day. The half-life period of the reactor is 15 days, method evaluation, overview
D-amino-acid oxidase
diagnostics
th enzyme is useful for measurement of D-serine contents in the brain via an implantable D-serine biosensor for in vivo monitoring
putrescine oxidase
diagnostics
enzyme can be used as biosensor for putrescine using dispersed multiwalled carbon nanotubes as ultra-microelectrodes ensuring a good electron transfer from the enzyme, method optimization, overview
protein-lysine 6-oxidase
diagnostics
LOX can serve as a predictive marker of lymph node metastasis and prognosis in esophageal squamous cell carcinoma. Overall survival rates of the patients with esophageal squamous cell carcinoma with high LOX expression are significantly lower than those of the patients with esophageal squamous cell carcinoma with low LOX expression
diamine oxidase
diagnostics
clinical impact of a commercial radioimmunoassay for determination of DAO activity for the diagnosis of histamine intolerance is evaluated in a prospective, multicentre study in 207 adult patients. No correlation between diamine oxidase serum levels and clinical status is be found
cytochrome-b5 reductase
diagnostics
potential use of the enzyme in diagnostic areas
chloride peroxidase (vanadium-containing)
diagnostics
the observed bactericidal and virucidal activity of the alkalophilic P395D/L241V/T343A mutant of vanadium chloroperoxidase is an important step forward in the application of this robust enzyme as a component in disinfection formulations
homogentisate 1,2-dioxygenase
diagnostics
crude enzyme preparations can be used for a spectrophotometric method for routine, sensitive determination of homogentisate in human urine
beta-carotene 15,15'-dioxygenase
diagnostics
genotyping AI rams for c.196C-T can be used in selection against the yellow fat trait
Cypridina-luciferin 2-monooxygenase
diagnostics
labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enyzme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2
Cypridina-luciferin 2-monooxygenase
diagnostics
Cypridina luciferase is used as bioluminescence reporter enzyme in yeast, bacterial, and mammalian cell-based assays, enzyme activity is measured with a luminometer by addition of native or synthetic luciferin, also known as Vargulin, 0.5 microM in 10 mM Tris-HCl, pH 7.4
Cypridina-luciferin 2-monooxygenase
diagnostics
far-red luminescence imaging technology to visualize tumor specific antigens on cell surfaces in vitro (human hepato-cellular carcinoma cell line Huh-7) and in living bodies (mice xenografted with human Delta-like protein-positive Huh-7 cells), based on a far-red fluorescent indocyanine derivative conjugated to biotinylated Cypridina luciferase linked to an anti-human Delta-like protein (Dlk-1) monoclonal antibody (embryonic antigens expressed on the surface of many cancer cells) via biotin-avidin interaction
[histone-H3]-lysine-9-demethylase
diagnostics
dimethylation of histone H3 at lysine 4 in combination with trimethylation of histone H3 at lysine 4 is associated with an activated transcriptional state. An increase of H3K4diMe in neoplastic tissues is predictive of clinical outcome. In particular, tumor recurrence occurs earlier in low-grade prostate carcinoma patients with low H3K4diMe, independently of other clinical and pathologic parameters. Similarly, in large cell and squamous cell carcinomas, low H3K4diMe expression correlates with short survival
procollagen-proline dioxygenase
diagnostics
activation of NF-kappaB is observed in colon cancer. PHD3 appears to be a tumor suppressor in colorectal cancer cells that inhibits IKKbeta/NF-kappaB signaling. Determination of PHD3 status could aid targeted therapy selection for patients with colorectal tumors that have increased NF-kappaB activity
procollagen-lysine 5-dioxygenase
diagnostics
PLOD expression is associated with human breast cancer progression, PLOD2 expression is specifically prognostic in breast cancer
phytanoyl-CoA dioxygenase
diagnostics
the substrate phytanoyl-CoA is difficult to handle because of its amphipathic properties. Commercially available alternative substrates, such as hexadecanoyl-CoA or isovaleryl-CoA are potentially usefull
phytanoyl-CoA dioxygenase
diagnostics
assays for Refsum‘s disease should not be based on PAHX activity alone. At least four different types of mutation cause loss of PAHX activity in vivo. Mutations to the peroxisomal targeting sequence do not affect catalytic function but probably affect targeting or degradation of the enzyme. A second group of mutations, including truncation and missense mutations, results in total loss of activity. A third group of mutations results in uncoupling of substrate oxidation from that of 2-oxoglutarate. A fourth group of mutations causes partial inactivation by hindering binding/utilization of the 2-oxoglutarate cosubstrate and/or iron(II) cofactor. From the therapeutic and biochemical view-point the latter two groups are particularly interesting since it may be possible to restore their activity with modified cosubstrates
salicylate 1-monooxygenase
diagnostics
usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses
bacterial luciferase
diagnostics
expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview; expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview
tyrosine 3-monooxygenase
diagnostics
tyrosine hydroxylase is frequently used as a marker of dopaminergic neuronal loss in animal models of Parkinson’s disease
dopamine beta-monooxygenase
diagnostics
genotype-controlled measurement of plasma DBH activity might be used as a potential biological marker of the response to trauma
superoxide dismutase
diagnostics
Cu,Zn-SOD is a urinary marker of hepatic necrosis, but not hepatic fibrosis, overview
superoxide dismutase
diagnostics
the enzyme might be used to develop a biosensor for the detection of antioxidants and free radical activity
[methionine synthase] reductase
diagnostics
the MTRR A66G polymorphism is a potential biomarker for cancer risk
xanthine oxidase
diagnostics
the enzyme activity detection in sepsis can be used for a negative prognosis in sepsis diagnosis, overview
iodotyrosine deiodinase
diagnostics
improvement of pre-clinical detection of iodotyrosine deiodinase deficiency during the neonatal time
nicotinamide N-methyltransferase
diagnostics
high enzyme expression levels in oral squamous cell carcinoma cells indicates a favourable prognosis in patients, whereas the absence of NNMT expression constitutes a hallmark of aggressive biological behaviour
nicotinamide N-methyltransferase
diagnostics
NNMT is useful as a biomarker of lung cancer, stages I-III. The sensitivity of the detection in non-small cell lung cancer cells at 90% specficity increases from 25% to 32% when NNMT is used in combination with carcinoembryonic antigen, method development, overview
nicotinamide N-methyltransferase
diagnostics
the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target
glycine N-methyltransferase
diagnostics
partially suitable to distinguish between benign and malign tumor forms
glycine N-methyltransferase
diagnostics
enzyme level suitable as prognostic marker for human cholangiocarcinoma and hepatocellular carcinoma
methylated-DNA-[protein]-cysteine S-methyltransferase
diagnostics
introduction of the MS-MLPA assay may not only be helpful for predicting response of gliomas to temozolomide, but may also facilitate tailor-made treatment with other chemotherapeutic agents for a variety of tumors
thiopurine S-methyltransferase
diagnostics
the chip-based miniaturization provides a clinically feasible genotyping platform for one-at-a-time testing
thiopurine S-methyltransferase
diagnostics
thiopurine S-methyltransferase genotypes or phenotypes may be a predictive factor for azathioprine-induced toxicities
25S rRNA (cytosine2870-C5)-methyltransferase
diagnostics
whereas protein p120 is almost undetectable in normal tissues, it is overexpressed in virtually all types of cancer cells and is therefore considered to be a predictive cancer marker
phosphoribosylglycinamide formyltransferase
diagnostics
increased expression of glycinamide ribonucleotide transformylase is associated with a poor prognosis in hepatocellular carcinoma
phosphoribosylglycinamide formyltransferase
diagnostics
GART expression is associated with glioma grade and high GART protein expression might be related to poor outcome
phosphoribosylaminoimidazolecarboxamide formyltransferase
diagnostics
enzyme variants are used to predict methotrexate response in juvenile idiopathic arthritis, overview
methylmalonyl-CoA carboxytransferase
diagnostics
monitoring propionibacteria population and propionic fermentation in human trials constitutes a crucial step in determining Propionibacterium freudenreichii ability to prevent intestinal disorders
ornithine carbamoyltransferase
diagnostics
the enzyme alone or in combination with other markers is a useful indicator for Kupffer cell activation as well as for mitochondrial damage in hepatic cells
ornithine carbamoyltransferase
diagnostics
ornithine carbamoyltransferase is one promising blood-based biomarker candidate for liver injury examination
ornithine carbamoyltransferase
diagnostics
the enzyme can be used as biomarker for early stage of hepatic injury induced by CCl4, diagnostics values are best in combination with total bile acid and phosphorylase measurements
choline O-acetyltransferase
diagnostics
ChAT is selected as a marker of cholinergicneurons. In the CA1 region of hippocampus of mice, several of the insulin signaling-related proteins are co-located with ChAT, and most double immunoreactive positive cells are pyramidal cells
[acyl-carrier-protein] S-malonyltransferase
diagnostics
coupled enzyme assay can be used in development and optimization of small-molecule inhibitors for antibacterial therapy
dihydrolipoyllysine-residue succinyltransferase
diagnostics
the rSucB may be a candidate antigen for a specific serological diagnosis of Bartonella henselae infection
phosphatidylcholine-retinol O-acyltransferase
diagnostics
enzyme might be a prognostic or therapeutic marker in renal cancer
phosphatidylcholine-retinol O-acyltransferase
diagnostics
the Lrat knockout mutant mice may serve as animal model with early onset severe retinal dystrophy and severe retinyl ester deprivation
sucrose-phosphate synthase
diagnostics
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
glucuronosyltransferase
diagnostics
androgen glucuronidation by UGT2B17 is particularly interesting in doping control
glucuronosyltransferase
diagnostics
3-epideacetycinobufagin can be used specially to measure the catalytic activity of UGT2B7 in biological samples due to its exclusive substrate specificty for the compound
N-acetyllactosaminide 3-alpha-galactosyltransferase
diagnostics
identification of N-acetyllactosamine moiety of glycoproteins and glycolipids by chemiluminescence with mutated enzymes
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
diagnostics
GCNT1 expression in prostate biopsy specimen is a significant and independent predictor of recurrence after radical prostatectomy, which can be used in pre-treatment decision making for the patient
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
diagnostics
possible role of GCNT3 gene expression as prognostic marker in colon cancer. Low CNT3 expression is a promising prognostic biomarker for colon cancer that could be used to identify early-stage colon cancer patients at high risk of relapse. The enzyme might also constitute a biomarker to monitor tumour response to chemotherapy in cancer patients
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
diagnostics
C2GnT1 might become a prognostic factor for endometrial carcinoma. Patients with C2GnT1 overexpression show significantly shorter survival, multivariable analysis also indicate that 2GnT1 overexpression is an independent prognostic factor
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
diagnostics
detection of core2 beta-1,6-N-acetylglucosaminyltransferase in post-digital rectal examination urine is a reliable indicator for extracapsular extension of prostate cancer. The number of GCNT1-positive cases is significantly lower in cases of organ-confined disease than in cases of extracapsular extension. GCNT1-negative tumors are a associated with significantly better prostate-specific antigen (PSA)-free survival compared with GCNT1-positive tumors. Multivariate analysis reveals that detection of GCNT1 expression is an independent risk factor for prostate-specific antigen recurrence
dolichyl-phosphate-mannose-protein mannosyltransferase
diagnostics
analysis of fibroblasts to elucidate the phenotypes of POMT1 mutations
alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase
diagnostics
the enzyme is useful as diagnostic marker for gastric adenocarcinomas: low intratumoral MGAT5 density is associated with poor differentiation, N classification, TNM stage, and Kiel stage, and is an independent prognosticator for poor overall survival. The combination of intratumoral MGAT5 expression and TNM or Kiel staging systems has a better predictive power for overall survival. Applying the prognostic value of intratumoral MGAT5 density to TNM stage III+IV and Kiel stage IIIB+IV groups shows a better risk stratification for overall survival in patients with late-stage gastric cancer, overview
glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
diagnostics
chondroitin sulfate synthase 1 expression is associated with malignant potential of soft tissue sarcomas with myxoid substance. High expression of CHSY1 is significantly associated with poorer patient outcomes
Results 1 - 100 of 424 > >>