Information on EC 1.4.3.3 - D-amino-acid oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.4.3.3
-
RECOMMENDED NAME
GeneOntology No.
D-amino-acid oxidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
ping pong bi bi mechanism
-
a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
catalytic mechanism, reductive half-reaction mechanism, wild-type and S19G/S120P/Q144R/K321M/A345V mutant enzymes, overview
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
Biosynthesis of antibiotics
-
-
D-Arginine and D-ornithine metabolism
-
-
glycine metabolism
-
-
Glycine, serine and threonine metabolism
-
-
L-lysine degradation V
-
-
Metabolic pathways
-
-
Penicillin and cephalosporin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
D-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD). Wide specificity for D-amino acids. Also acts on glycine.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-aminoacid oxidase
-
-
-
-
DAAO
-
-
-
-
DAMOX
-
-
-
-
DAO
-
-
-
-
ophio-amino-acid oxidase
-
-
-
-
oxidase, D-amino acid
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9000-88-8
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
putative D-amino acid oxidase-2
UniProt
Manually annotated by BRENDA team
Norwegian schizophrenia patients
-
-
Manually annotated by BRENDA team
recombinant
-
-
Manually annotated by BRENDA team
C57BL/6J mice
-
-
Manually annotated by BRENDA team
Male Slc:ddy mice
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
C57BL/6J mice
-
-
Manually annotated by BRENDA team
male F344 Fischer, Wistar, and Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
male Sprague Dawley rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
male Wistar rats
-
-
Manually annotated by BRENDA team
DAO1; i.e. Rhodosporidium toruloides
UniProt
Manually annotated by BRENDA team
formerly Rhodotorula gracilis
UniProt
Manually annotated by BRENDA team
putative; strain DSM 9941
UniProt
Manually annotated by BRENDA team
strain CBS 4095
-
-
Manually annotated by BRENDA team
Trigonopsis variabilis ATCC 10679
-
-
-
Manually annotated by BRENDA team
Trigonopsis variabilis CBS 4095
CBS 4095
-
-
Manually annotated by BRENDA team
Trigonopsis variabilis CBS 4095
strain CBS 4095
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism
malfunction
-
increased level of D-serine resulting from decreased catalysis correct the performance of mice with deficient NMDAR glycine site activation in behavioral tasks relevant to the negative and cognitive symptoms of schizophrenia
malfunction
-
the DAAO risk gene is associated with schizophrenia, linkage between DAAO and schizophrenia in a severely affected subpopulation of schizophrenia patients, genotyping, expression analysis, and detailed overview
malfunction
-
a total loss of enzymatic activity is associated with the adult onset of familial amyotrophic lateral sclerosis. Altered enzyme expression levels and activity have been reported in schizophrenia
physiological function
-
D-amino acid oxidase is an enzyme catalyzing oxidative deamination of neutral and polar D-amino acids. It plays a role in neuropathic pain, in spinal nerve ligation-induced mechanical allodynia, which is specifically blocked by sodium benzoate
physiological function
-
DAAO is involved in the degradation of the gliotransmitter D-serine, an important modulator of NMDA-receptor-mediated neurotransmission. An increase in DAAO activity, yielding a decrease in D-serine concentration, is among the molecular mechanisms leading to the onset of schizophrenia susceptibility, overview
physiological function
-
DAAO plays a key role in the pathophysiology of schizophrenia
physiological function
-
DAO may regulate D-amino acid concentration by modulating the cerebrospinal fluid
physiological function
-
physiological role of D-amino acids and DAAOs, regulation of the nervous system, hormone secretion, and other processes by D-amino acids, detailed overview
physiological function
-
spinal D-amino acid oxidase contributes to the pathogenesis of hyperalgesia
physiological function
-
the enzymatic function of DAAO is required for AHR activation by D-Trp and D-Tyr. DAAO catalyzes the production of aryl hydrocarbon receptor, AHR, agonists through the enzymatic conversion of D-tryptophan to indole-3-pyruvic acid, followed by nonenzymatic oxidation and condensation of indole-3-pyruvic acid is a critical step in the generation of receptor agonists by DAAO and aspartate aminotransferase, AST. Products of this process include the two agonists, 1,3-di(1H-indol-3-yl)propan-2-one and 1-(1H-indol-3-yl)-3-(3H-indol- 3-ylidene) propan-2-one, overview
physiological function
-
the enzyme is involved in glutamate receptor function, reported associations with negative symptoms and with anxiety and depression, respectively
physiological function
-
the enzyme is involved in the conversion of cephalosporin C
physiological function
-
the enzyme is involved in the development of schizophrenia, mechanism, overview
physiological function
-
the enzyme modulates the function of the N-methyl D-aspartate receptor contributing to the involvement of the receptor signalling in schizophrenia
physiological function
-
D-amino acid oxidase is a FAD-containing flavoprotein that catalyzes the oxidative deamination of D-amino acids with O2 to generate the corresponding 2-oxo acids, along with H2O2 and NH3
physiological function
-
DAO is associated with schizophrenia. DAO modulates neurotransmission by metabolizing the D-serine, that binds to the coagonist-binding site of N-methyl-D-aspartate subtype of glutamate receptors and enhances the neurotransmission
physiological function
-
DAO is associated with schizophrenia. DAO modulates neurotransmission by metabolizing the D-serine, that binds to the coagonist-binding site of N-methyl-D-aspartate subtype of glutamate receptors and enhances the neurotransmission. DAO might be involved in D-serine-induced nephrotoxicity only in rats
physiological function
-
in human brain the flavoprotein D-amino acid oxidase is responsible for the degradation of the neuromodulator D-serine, an important effector of NMDA-receptor mediated neurotransmission
physiological function
-
D-amino acid oxidase functions in metamorphosis
physiological function
-
expression of a D-amino acid oxidase gene from Schizosaccharomyces pombe in tobacco chloroplasts give rise to transplastomic ptdao plants that resemble wild-type plants in growth and appearance. Growth of ptdao cells is tolerant to D-alanine but inhibited by D-valine
physiological function
-
the enzyme is part responsible for the significant increases in brain kynurenic acid levels seen under inflammatory conditions
metabolism
-
D-serine is synthesized from L-serine by serine racemase and degraded by D-amino acid oxidase in neurons, overview
additional information
-
metabolism of extracellular D-serine and effects of D-serine metabolites with pathophysiological role of DAO, overexpression of DAO in astroglial cells induces the enhanced cytotoxicity, reaction product beta-hydroxypyruvate also induces cell death, comprising apoptosis, in the astroglial cell, but not in the other cells derived from liver and kidney, overview
additional information
-
mutation R199W in the D-amino acid oxidase gene, DAO is associated with classical adult onset familial amyotrophic lateral sclerosis, FALS in a three generational FALS kindred, the 14.52 cMregiononchromosome 12q22-23 is linked to disease. Lentiviral-mediated expression of R199WDAO in primary motor neuron cultures causes increased TUNEL labeling. This effect also occurs in motor neurons cocultured on transduced astrocytes expressing R199W, indicating that the motor neuron cell death induced by this mutation is mediated by both cell autonomous and noncell autonomous processes
additional information
-
phenotypes of mutant mice resulting from enhanced N-methyl-D-aspartate receptor function, accumulation of D-amino acids in the organs and body fluids of mutant mice, overview. Mutant mice lacking DAO do not show great abnormalities, but responses to the nociceptive stimuli are different between the mutant and wild-type mice. The function of NMDA receptors is enhanced in the mutant mice, with long-term potentiation in the CA1 area of hippocampus being augmented. Mutant mice display an anxiety-like behavior and have aminoaciduria
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-D-phenylalanine + H2O + O2
3,4-dihydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
4-fluoro-DL-phenylalanine + H2O + O2
4-fluoro-phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q7X2D3
81% activity compared to D-methionine
-
-
?
a D-amino acid + H2O + O2
a 2-oxo acid + NH3 + H2O2
show the reaction diagram
-
D-amino-acid oxidase catalyzes the oxidative deamination of D-amino acids, stereoisomers of the naturally occurring L-amino acids
-
-
?
alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C
alpha-ketoadipinyl-7-aminocephalosporanic acid
show the reaction diagram
-
-
-
-
-
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
Q1AYM8
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
Q9RIA4
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
P24552
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
P00371
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
P80340
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
1% of the activity with D-Ala
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
1% of the activity with D-Met
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
13% of the activity with D-Val
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Met
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
14% activity compared to D-alanine
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
36% activity compared to D-alanine
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
Trigonopsis variabilis CBS 4095
-
-
-
-
?
cephalosporin C + H2O + O2
7-aminocephalosporanic acid + ? + H2O2
show the reaction diagram
-
conversion of cephalosporin C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO
-
-
?
cephalosporin C + H2O + O2
7-aminocephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-1-naphthyl-alanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-1-naphthyl-glycine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-2-aminobutyrate + H2O + O2
2-oxobutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-2-chloro-phenylglycine + H2O + O2
(2-chlorophenyl)acetic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-2-fluoro-phenylglycine + H2O + O2
(2-fluorophenyl)acetic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-2-naphthyl-alanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-2-naphthyl-glycine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
?
show the reaction diagram
-
i.e. D-Dopa, D-Dopa undergoes unidirectional chiral inversion and further suggest that D-Dopa is first oxidatively deaminated by DAAO to its alpha-keto acid and then transaminated by dopa transaminase to L-Dopa
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
? + H2O2 + NH3
show the reaction diagram
-
best substrate
-
-
?
D-4-chloro-phenylglycine + H2O + O2
(4-chlorophenyl)acetic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-4-fluoro-phenylglycine + H2O + O2
(4-fluorophenyl)acetic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-4-hydroxy-phenylglycine + H2O + O2
(4-hydroxyphenyl)acetic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q9HGY3
best substrate
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
40% of the activity with D-Pro
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
57% of the activity with D-Met
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
71% of the activity with D-Met
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
97% of the activity with D-Val
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
104% activity compared to D-Ser
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
165% activity compared to D-Ser
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q6TGN2
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
P18894
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
P00371
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
P80340
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q6NY97
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
A0PFJ3
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
highly active
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
enzyme works as a metabolizing agent of exogenous and endogenous free D-Ala that is abundant in aquatic invertebrates such as crustaceans and bivalve mollusks, which are potential food sources of the fish. After oral administration of D-Ala at 0.005 mM/g body weight per day to carp for 30 days, enzyme activity increases by about 8fold, 3fold, and 1.5fold in intestine, hepatopancreas, and kidney, respectively, whereas no increase is found in brain
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q6TGN2
the inducible enzyme plays an important physiological role in metabolizing exogenous D-Ala that is abundant in their prey invertebrates, crustaceans, and mollusks
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q7X2D3
0.26% activity compared to D-methionine
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
A0PFJ3
recombinant maltose-binding protein-fusion DAAO
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
the D-amino acid is a modulator of the N-methyl-D-aspartate receptor mediated neurotransmission
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
5.8% activity in tadpoles compared to D-proline
-
-
?
D-alanine + H2O + O2
pyruvate + H2O2 + NH3
show the reaction diagram
-
-
-
-
?
D-allo-isoleucine + H2O + O2
?
show the reaction diagram
Q7X2D3
1.0% activity compared to D-methionine
-
-
?
D-alpha-aminoadipate + H2O + O2
2-oxoadipate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
via formation of an imino acid
-
-
?
D-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Met
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
25% of the activity with D-Ala
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Pro
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
43% of the activity with D-Val
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
7% of the activity with D-Ala
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
19% activity compared to D-alanine
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
42% activity compared to D-alanine
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
80% activity compared to D-methionine
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
Q9HGY3
0.18% of the activity with D-Ala
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Ala
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
-
3% of the activity with D-Ala
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
-
40% of the activity with D-Met
-
-
?
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
show the reaction diagram
-
65% of the activity with D-Val
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
7% of the activity with D-Met
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
38.6% activity compared to D-Ser
-
-
?
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
5.5% activity compared to D-Ser
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
38% activity compared to D-alanine
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
67% activity compared to D-alanine
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
2% activity compared to D-alanine
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
3% activity compared to D-alanine
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
1.0% activity in tadpoles compared to D-proline
-
-
?
D-aspartate + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-aspartate + H2O + O2
? + NH3 + H2O2
show the reaction diagram
A0PFJ3
recombinant maltose-binding protein-fusion DAAO
-
-
?
D-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
weak activity
-
-
?
D-citrulline + H2O + O2
2-oxo-5-ureidopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Cys + H2O + O2
2-oxo-3-thiopropionate + NH3 + H2O2
show the reaction diagram
-
1% of the activity with D-Met
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
18% activity compared to D-alanine
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
9% activity compared to D-alanine
-
-
?
D-DOPA + H2O + O2
3,4-dihydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-DOPA + H2O + O2
3,4-dihydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
P14920
the maximal velocity for oxidation of D-DOPA is much greater than for D-serine
-
-
?
D-ethionine + H2O + O2
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ethionine + H2O + O2
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
1% of the activity with D-Pro
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
15% of the activity with D-Ala
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
5% of the activity with D-Ala
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
51% of the activity with D-Met
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
53% of the activity with D-Met
-
-
?
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
81% of the activity with D-Val
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
10% of the activity with D-Met
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Met
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Pro
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
54% of the activity with D-Ala
-
-
?
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
9% of the activity with D-Val
-
-
?
D-glufosinate + H2O + O2
?
show the reaction diagram
-
-
-
-
ir
D-glutamate + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamate + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
3% activity compared to D-alanine
-
-
?
D-glutamate + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
9% activity compared to D-alanine
-
-
?
D-glutamic acid + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
58% activity compared to D-alanine
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
78% activity compared to D-alanine
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
14% of the activity with D-Ala
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
3% of the activity with D-Pro
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Ala
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
58% of the activity with D-Met
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
88% of the activity with D-Val
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
Q7X2D3
5.9% activity compared to D-methionine
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
60% activity compared to D-alanine
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
69% activity compared to D-alanine
-
-
?
D-homo-phenylalanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
15% of the activity with D-Met
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
19% of the activity with D-Ala
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
35% of the activity with D-Pro
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
56% of the activity with D-Met
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
76% of the activity with D-Val
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
60% activity compared to D-alanine
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
69% activity compared to D-alanine
-
-
?
D-kynurenine + H2O + O2
kynurenic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Leu + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
21% of the activity with D-Pro
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
29% of the activity with D-Ala
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
32% of the activity with D-Val
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
42% of the activity with D-Met
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
57% of the activity with D-Met
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
Q9HGY3
7% of the activity with D-Ala
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
92% of the activity with D-Ala
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
12% activity compared to D-methionine
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
37% activity compared to D-alanine
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
86% activity compared to D-alanine
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
2.3% activity in tadpoles compared to D-proline
-
-
?
D-Lys + H2O + O2
?
show the reaction diagram
-
17% of the activity with D-Val
-
-
?
D-Lys + H2O + O2
?
show the reaction diagram
-
2% of the activity with D-Pro
-
-
?
D-Lys + H2O + O2
?
show the reaction diagram
-
25% of the activity with D-Ala
-
-
?
D-Lys + H2O + O2
?
show the reaction diagram
-
5% of the activity with D-Met
-
-
?
D-Lys + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
poor substrate
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
7% activity compared to D-alanine
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
-
8% activity compared to D-alanine
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
99% activity compared to D-methionine
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Q9HGY3
14% of the activity with D-Ala
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
28% of the activity with D-Ala
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
75% of the activity with D-Pro
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
78% of the ativity with D-Val
-
-
?
D-Met + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-methionine + 2,6-dichloroindophenol
4-methylsulfanyl-2-oxobutanoate + reduced 2,6-dichloroindophenol
show the reaction diagram
Trigonopsis variabilis, Trigonopsis variabilis ATCC 10679
-
-
-
-
?
D-methionine + 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
-
-
-
?
D-methionine + 2,6-dichlorophenolindophenol + H2O
?
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
P00371
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
100% activity
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
113% activity compared to D-alanine
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
77% activity compared to D-alanine
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
by far best substrate
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
7.6% activity in tadpoles compared to D-proline
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
highly active
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
with 6-dichloroindophenol as electron acceptor
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Trigonopsis variabilis ATCC 10679
-
with 6-dichloroindophenol as electron acceptor
-
-
?
D-methionine + H2O + O2 ap
2-oxo-4-methylthiobutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-naphthylalanine + H2O + O2
naphthylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ornithine + H2O + O2
5-amino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ornithine + H2O + O2
5-amino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
Q7X2D3
15% activity compared to D-methionine
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q9HGY3
1.5% of the activity with D-Ala
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
19% of the activity with D-Ala
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
36% of the activity with D-Val
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
41% of the activity with D-Ala
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
50% of the activity with D-Met
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
79% of the activity with D-Met
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
84% of the activity with D-Pro
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
Trigonopsis variabilis, Trigonopsis variabilis CBS 4095
-
activity of the DAAO after incubation in water-insoluble ionic liquids is higher than in water-soluble ones
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
P14920
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
second best substrate
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
44% activity compared to D-alanine
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q7X2D3
54% activity compared to D-methionine
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
Q7X2D3
80% activity compared to D-methionine
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
94% activity compared to D-alanine
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
conversion of D-phenylalanine to 99.0% phenylpyruvate within 100 min
-
-
?
D-phenylalanine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
D-phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
D-Pro + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Pro + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Pro + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Pro + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Pro + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q9HGY3
1.4% of the activity with D-Ala
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
22% of the activity with D-Ala
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
25% of the activity with D-Val
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Met
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
41% of the activity with D-Ala
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
57% of the activity with D-Met
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
0.25% activity compared to D-methionine
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
21% activity compared to D-alanine
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
46% activity compared to D-alanine
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
1.1% activity in tadpoles compared to D-proline
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
P00371
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
best substrate, 100% activity in tadpoles and adults
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
100% activity
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
18% of the activity with D-Ala
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
18% of the activity with D-Pro
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
22% of the activity with D-Val
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
Q9HGY3
32% of the activity with D-Ala
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
41% of the activity with D-Met
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
6% of the activity with D-Ala
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
9% of the activity with D-Met
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
P14920
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
astroglial enzyme plays an important role in metabolizing a neuromodulator, D-serine
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
23% activity compared to D-alanine
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
49% activity compared to D-alanine
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
7.6% activity in tadpoles compared to D-proline
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
poor substrate
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
D-serine, an endogenous agonist of the N-methyl-D-aspartate, NMDA, receptors, is effective in the treatment of schizophrenia. However, orally administered D-serine is metabolized substantially by D-amino acid oxidase diminishing its oral bioavailability
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
high doses of D-serine attenuate both amphetamine-induced psychomotor activity and dopamine release and also improve performance in novel object recognition
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
metabolizaation of the N-methyl D-aspartate receptor co-agonist
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
physiological effects of D-serine, overview
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
the D-amino acid is a modulator of the N-methyl-D-aspartate receptor mediated neurotransmission
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
low catalytic efficiency and substrate affinity on the physiological substrate D-serine
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
Mus musculus C57BL/6J
-
physiological effects of D-serine, overview
-
-
?
D-serine + H2O + O2
3-hydroxypyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
D-Thr + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
18% activity compared to D-alanine
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
2% activity compared to D-alanine
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
poor substrate
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
Q9HGY3
1.2% of the activity with D-Ala
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
10% of the activity with D-Met
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Ala
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Pro
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyrate + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Val
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
14% of the activity with D-Ala
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Pro
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
38% of the activity with D-val
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
56% of the activity with D-Met
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
9% of the activity with D-Ala
-
-
?
D-Trp + H2O + O2
indol-3-pyruvate+ NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
129% activity compared to D-alanine
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
92% activity compared to D-alanine
-
-
?
D-tryptophan + H2O + O2
indole-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indole-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
DAAO catalyzes the production of aryl hydrocarbon receptor, AHR, agonists through the enzymatic conversion of D-tryptophan to indole-3-pyruvic acid
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
17% of the activity with D-Val
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
26% of the activity with D-Met
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
4% of the activity with D-Pro
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
6% of the activity with D-Ala
-
-
?
D-Tyr + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
8% of the activity with D-Ala
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
P14920
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
18% activity compared to D-alanine
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
19% activity compared to D-alanine
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
4.7% activity compared to D-methionine
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
15% of the activity with D-Ala
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
28% of the activity with D-Pro
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
41% of the activity with D-Met
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
62% of the activity with D-Ala
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
Q9HGY3
8% of the activity with D-Ala
-
-
?
D-Val + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
95% of the activity with D-Met
-
-
?
D-Val + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
best substrate
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
P80340
best substrate
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
Q7X2D3
0.35% activity compared to D-methionine
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
60% activity compared to D-alanine
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
63% activity compared to D-alanine
-
-
?
D-valine + H2O + O2
alpha-ketoisovalerate + NH3 + H2O2
show the reaction diagram
-
highly active
-
-
?
DL-2-naphthyl-alanine + H2O + O2
L-2-naphthyl-alanine + 2-naphthylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
DL-homophenylalanine + H2O + O2
homophenylpyruvate + NH3 + H2O2
show the reaction diagram
Q7X2D3
31% activity compared to D-methionine
-
-
?
DL-tert-leucine + H2O + O2
?
show the reaction diagram
Q7X2D3
0.12% activity compared to D-methionine
-
-
?
epsilon-N-benzoyl-D-lysine + H2O + O2
6-benzylamino-2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
Gly + H2O + O2
acetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
Gly + H2O + O2
acetate + NH3 + H2O2
show the reaction diagram
-
15% of the activity with D-Ala
-
-
?
Gly + H2O + O2
acetate + NH3 + H2O2
show the reaction diagram
-
2% of the activity with D-Met
-
-
?
Gly + H2O + O2
acetate + NH3 + H2O2
show the reaction diagram
-
3% of the activity with D-Met
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
poor substrate
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
2% activity compared to D-alanine
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
3% activity compared to D-alanine
-
-
?
glycine + H2O + O2
glyoxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
N-acetyl-D-alanine + H2O + O2
pyruvate + aminoacetate + H2O2
show the reaction diagram
-
-
-
-
?
N-methyl-D-aspartate + H2O + O2
?
show the reaction diagram
P80324
wild-type enzyme shows no activity, mutant enzyme M213R is active
-
-
?
N-methyl-DL-leucine + H2O + O2
?
show the reaction diagram
Q7X2D3
2.1% activity compared to D-methionine
-
-
?
O-methyl-DL-serine + H2O + O2
?
show the reaction diagram
Q7X2D3
0.33% activity compared to D-methionine
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
thiazolidine-2-carboxylic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
thiazolidine-2-carboxylic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-DOPA + H2O + O2
3,4-dihydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
first enzyme involved in the catabolism of D-amino acids. The inducer D-Ala acts by increasing the rate of DAAO mRNA transcription. Ammonium sulfate appears to have a negative effect on DAAO mRNA translation and on the expression of DAAO activity. The best expression of DAAO activity is obtained by growing the cells for 12 h at 30C in the presence of glucose and D-alanine using cell pre-cultured for 10 h on glucose and L-Ala
-
-
-
additional information
?
-
Q9HGY3
no activity with D-Trp, D-Tyr, D-Cys, D-Gln, D-Lys, D-Arg, D-His and D-Asp
-
-
-
additional information
?
-
-
the amount of the neutral D-amino acids are regulated, each D-amino acid is regulated in a different way
-
-
-
additional information
?
-
-
activity with D-Asp is not detectable
-
-
-
additional information
?
-
-
best substrate is by far D-methionine followed by D-phenylalanine, D-tryptophan, D-valin, and D-alanine
-
-
-
additional information
?
-
P80340
best substrate is D-valine followed by D-tryptophan, D-phenylalanine, D-alanine, and D-cysteine
-
-
-
additional information
?
-
-
D-aspartate and D-glutamate are no substrates
-
-
-
additional information
?
-
P80340
D-aspartate and D-glutamate are no substrates
-
-
-
additional information
?
-
-
D-proline is the best substrate followed by D-methionine, D-alanine, D-norleucine, D-isoleucine, and D-phenylalanine
-
-
-
additional information
?
-
-
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
Q1AYM8
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
Q9RIA4
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
-
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
P24552
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
P80324
DAAOs can be divided into two groups regarding their substrate specificity, the first group prefers amino acids with small apolar side chains (D-Ala is the best substrate), the second group prefers D-amino acids possessing large hydrophobic side chains such as D-Trp, D-Met, D-Val, and D-Phe, usually the small amino acid Gly and the charged (acidic or basic) amino acids are poor DAAO substrates
-
-
-
additional information
?
-
-
nearly inactive toward D-aspartic and D-glutamic acids
-
-
-
additional information
?
-
Q7X2D3
no activity with glycine, D-serine, D-asparagine, D-aspartate, D-glutamate, D-threonine, D-tryptophan, and cephalosporin C
-
-
-
additional information
?
-
P00371
the enzyme exhibits very low activity towards basic amino acids, and it does not oxidize those with an acidic side chain
-
-
-
additional information
?
-
-
the wild-type DAAO is mainly active on neutral D-amino acids, while basic D-amino acids are poor substrates and the acidic ones are virtually not oxidized
-
-
-
additional information
?
-
-
D-amino acid oxidase is an enzyme catalyzing the strict stereospecificity the oxidative deamination of neutral and polar D-amino acids to alpha-keto acids, NH3 and hydrogen peroxide
-
-
-
additional information
?
-
-
DAAO is a catabolic flavoenzyme that catalyzes the oxidative deamination of D-amino acids to the corresponding a-keto acids, hydrogen peroxide, and ammonia. DAAO is strictly specific for D-isomers of amino acids
-
-
-
additional information
?
-
-
DAO catalyzes the oxidative degradation of most of the D-amino acids in mammals
-
-
-
additional information
?
-
-
the enzyme is involved in the conversion of cephalosporin C
-
-
-
additional information
?
-
-
the mechanism of the enzyme regulation is complex and multi-parametric because the same enzyme simultaneously influences the level of different D-amino acids, which can result in opposing effects, overview
-
-
-
additional information
?
-
-
D-amino acid oxidase is a flavoenzyme that catalyzes the oxidation of D-amino acids to the corresponding imino acids and hydrogen peroxide
-
-
-
additional information
?
-
-
DAAO is a flavoprotein that catalyzes the dehydrogenation of different D-amino acids to their imino counterparts via a reduced flavin product complex. The reduced flavin is then reoxidized by O2 to yield H2O2, whereas the imino acid spontaneously hydrolyzes to the corresponding keto acid and NH4+. DAAO is strictly stereospecific and oxidizes a variety of D-amino acids, with a preference for those having small hydrophobic side chains, followed by those bearing polar, aromatic, and basic groups
-
-
-
additional information
?
-
-
hDAAO exhibits optimal activity toward neutral D-amino acids and marginal activity toward basic ones, while acidic D-amino acids are not oxidized, structure-function relationship analysis, overview
-
-
-
additional information
?
-
A0PFJ3
recombinant maltose-binding protein-fusion DAAO can oxidize D-alanine and D-aspartate, but not D-leucine, D-isoleucine, and D-serine
-
-
-
additional information
?
-
-
the enzyme catalyzes oxidative deamination of D-amino acids yielding hydrogen peroxide and an imino acid. The latter is further non-enzymatically hydrolyzed to an alpha-keto acid and ammonium. DAAO is highly specific towards D-isomers of amino acids, it is almost inactive towards the corresponding L-isomer
-
-
-
additional information
?
-
-
the enzyme catalyzes oxidative deamination of D-amino acids yielding hydrogen peroxide and an imino acid. The latter is further non-enzymatically hydrolyzed to an alpha-keto acid and ammonium. DAAO is highly specific towards D-isomers of amino acids, it is almost inactive towards the corresponding L-isomer. The wild-type enzyme is inactive towards D-Asp, being however very active with D-Ala
-
-
-
additional information
?
-
-
DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine
-
-
-
additional information
?
-
-
the flavoenzyme catalyzes the oxidative deamination of neutral and basic D-amino acids to the corresponding 2-oxo acids and H2O2 with concomitant reduction of FAD
-
-
-
additional information
?
-
-
comparison of the kinetics of trypsin cleavage of hDAAO in the presence of various ligands, overview
-
-
-
additional information
?
-
-
conversion of cephalosporine C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO
-
-
-
additional information
?
-
-
DAO shows no activity with D-Asp, N-methyl-D-Asp, and D-Glu
-
-
-
additional information
?
-
-
specific dynamic channel for the diffusion of O2 leads from solvent to the flavin Si-side. O2 affinity, catalytic center, and substrate binding of wild-type and mutant enzymes, molecular dynamics simulations built from the three-dimensional structure, PDB ID 1c0p, in which the ligand D-Ala is replaced by imino pyruvate, overview
-
-
-
additional information
?
-
-
the enzyme interacts with the mammalian protein modulator pLG72, D-aspartate and glycine are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a D-amino acid + H2O + O2
a 2-oxo acid + NH3 + H2O2
show the reaction diagram
-
D-amino-acid oxidase catalyzes the oxidative deamination of D-amino acids, stereoisomers of the naturally occurring L-amino acids
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-2-aminobutyrate + H2O + O2
2-oxobutyrate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
?
show the reaction diagram
-
D-Dopa undergoes unidirectional chiral inversion and further suggest that D-Dopa is first oxidatively deaminated by DAAO to its alpha-keto acid and then transaminated by dopa transaminase to L-Dopa
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
? + H2O2 + NH3
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
A0PFJ3
-
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
enzyme works as a metabolizing agent of exogenous and endogenous free D-Ala that is abundant in aquatic invertebrates such as crustaceans and bivalve mollusks, which are potential food sources of the fish. After oral administration of D-Ala at 0.005 mM/g body weight per day to carp for 30 days, enzyme activity increases by about 8fold, 3fold, and 1.5fold in intestine, hepatopancreas, and kidney, respectively, whereas no increase is found in brain
-
-
?
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
Q6TGN2
the inducible enzyme plays an important physiological role in metabolizing exogenous D-Ala that is abundant in their prey invertebrates, crustaceans, and mollusks
-
-
?
D-alanine + H2O + O2
pyruvate + H2O2 + NH3
show the reaction diagram
-
-
-
-
?
D-alpha-aminoadipate + H2O + O2
2-oxoadipate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
ir
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Arg + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
weak activity
-
-
?
D-citrulline + H2O + O2
2-oxo-5-ureidopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ethionine + H2O + O2
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ethionine + H2O + O2
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamic acid + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-kynurenine + H2O + O2
kynurenic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
P80324
-
-
-
?
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
Trigonopsis variabilis ATCC 10679
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-ornithine + H2O + O2
5-amino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
-
astroglial enzyme plays an important role in metabolizing a neuromodulator, D-serine
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
D-serine, an endogenous agonist of the N-methyl-D-aspartate, NMDA, receptors, is effective in the treatment of schizophrenia. However, orally administered D-serine is metabolized substantially by D-amino acid oxidase diminishing its oral bioavailability
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
high doses of D-serine attenuate both amphetamine-induced psychomotor activity and dopamine release and also improve performance in novel object recognition
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
metabolizaation of the N-methyl D-aspartate receptor co-agonist
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
physiological effects of D-serine, overview
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
-
low catalytic efficiency and substrate affinity on the physiological substrate D-serine
-
-
?
D-serine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
show the reaction diagram
Mus musculus C57BL/6J
-
physiological effects of D-serine, overview
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-threonine + H2O + O2
2-oxo-3-hydroxybutyric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indol-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tryptophan + H2O + O2
indole-3-pyruvic acid + NH3 + H2O2
show the reaction diagram
-
DAAO catalyzes the production of aryl hydrocarbon receptor, AHR, agonists through the enzymatic conversion of D-tryptophan to indole-3-pyruvic acid
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + O2
4-hydroxyphenylpyruvic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
D-valine + H2O + O2
alpha-ketoisovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
thiazolidine-2-carboxylic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
-
thiazolidine-2-carboxylic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
glycine + 2 H2O + O2
2 formic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
first enzyme involved in the catabolism of D-amino acids. The inducer D-Ala acts by increasing the rate of DAAO mRNA transcription. Ammonium sulfate appears to have a negative effect on DAAO mRNA translation and on the expression of DAAO activity. The best expression of DAAO activity is obtained by growing the cells for 12 h at 30C in the presence of glucose and D-alanine using cell pre-cultured for 10 h on glucose and L-Ala
-
-
-
additional information
?
-
-
the amount of the neutral D-amino acids are regulated, each D-amino acid is regulated in a different way
-
-
-
additional information
?
-
-
D-amino acid oxidase is an enzyme catalyzing the strict stereospecificity the oxidative deamination of neutral and polar D-amino acids to alpha-keto acids, NH3 and hydrogen peroxide
-
-
-
additional information
?
-
-
DAAO is a catabolic flavoenzyme that catalyzes the oxidative deamination of D-amino acids to the corresponding a-keto acids, hydrogen peroxide, and ammonia. DAAO is strictly specific for D-isomers of amino acids
-
-
-
additional information
?
-
-
DAO catalyzes the oxidative degradation of most of the D-amino acids in mammals
-
-
-
additional information
?
-
-
the enzyme is involved in the conversion of cephalosporin C
-
-
-
additional information
?
-
-
the mechanism of the enzyme regulation is complex and multi-parametric because the same enzyme simultaneously influences the level of different D-amino acids, which can result in opposing effects, overview
-
-
-
additional information
?
-
-
DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine
-
-
-
additional information
?
-
-
the flavoenzyme catalyzes the oxidative deamination of neutral and basic D-amino acids to the corresponding 2-oxo acids and H2O2 with concomitant reduction of FAD
-
-
-
additional information
?
-
-
the enzyme interacts with the mammalian protein modulator pLG72
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
1 FAD per monomer of MW 38000-39000; flavoprotein
FAD
-
flavoprotein
FAD
-
flavoprotein; tightly bound
FAD
-
flavoprotein
FAD
-
1 FAD per monomer of MW 38000-39000; flavoprotein
FAD
-
flavoprotein; tightly bound
FAD
-
1 FAD per monomer of MW 38000-39000
FAD
-
mutant enzyme DELTASer308-Lys321 retains the binding of the FAD coenzyme, binding of mutant apoenzyme is weaker than that of wild-type apoenzyme to FAD
FAD
-
bound in an elongated conformation in the core of the enzyme
FAD
-
FAD molecule is tightly bound to each 40000 Da subunit
FAD
-
the enzyme contains one molecule of non-covalently bound FAD per protein monomer, which can be easily isolated from the apoprotein by dialysis in the presence of 1 M KBr
FAD
-
human enzyme weakly binds FAD and shows a significantly slower rate of flavin reduction compared to porcine enzyme
FAD
-
contains one molecule of noncovalently bound FAD per subunit
FAD
-
contains a molecule of noncovalently bound FAD per subunit
FAD
P24552
contains one molecule of noncovalently bound FAD per subunit
FAD
Q9RIA4
contains one molecule of noncovalently bound FAD per subunit
FAD
-
contains one molecule of noncovalently bound FAD per subunit
FAD
Q1AYM8
contains one molecule of noncovalently bound FAD per subunit
FAD
-
contains one molecule of noncovalently bound FAD per subunit
FAD
-
dependent, dissociation of FAD is a main contributor to the loss of activity
FAD
-
contains non-covalently bound FAD
FAD
-
added FAD does not stabilize DAO at and below 35C, but it contributes up to 3.6fold extra stability to the enzyme activity at temperatures higher than 35C
FAD
-
flavoprotein
FAD
-
dependent
FAD
P80340
dependent
FAD
-
dependent
FAD
Q6NY97
-
FAD
-
FAD-containing flavoenzyme
FAD
-
FAD-dependent enzyme
FAD
A0PFJ3
dependent on
FAD
-
flavoenzyme
FAD
-
flavoenzyme
FAD
-
flavoenzyme
FAD
-
DAAO is a flavoprotein, the cofactor is loosely bound
FAD
-
flavoenzyme, weak interaction with the flavin cofactor in the free form,the cofactor binding is significantly tighter in the presence of an active site ligand. hDAAO exists as an equilibrium of holo- and apoprotein forms in solution
FAD
-
the apoprotein form of hDAAO binds the substrate D-serine, this interaction increases FAD binding thus increasing the amount of active holoenzyme in solution, binding analysis of FAD, overview
flavin
-
the mutant enzyme M213R has a more polar microenvironment surrounding the flavin coenzyme
flavin
-
flavoenzyme
flavin
-
flavoenzyme
flavin
-
flavoenzyme
flavin
-
flavoenzyme
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe
-
2 identical subunits, each carrying probably 1 molecule of iron
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine
-
MK-801, a drastic decline in the expression of DAO mRNA is observed in most brain areas 1 h after the MK-801 administration (1.6 mg/kg)
(5R)-4-hydroxy-3,5-dimethyl-5-[(1E)-2-methyl-1,3-butadienyl]-2(5H)-thiophenone
-
i.e. thiolactomycin, competitive inhibition, competes with both the substrate and the coenzyme FAD
1,3-Butadien 1-carboxylic acid
-
D-phenylglycine oxidation
1,4-dihydropyrrolo[3,2-c]pyrazole-5-carboxylic acid
-
-
1H-indole-2-carboxylic acid
-
-
1H-pyrrole-2-carboxylic acid
-
-
2,3-dimethyl-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-(2,4-dichlorophenyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-(3-chlorophenyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-(4-chlorophenyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-(hydroxymethyl)-5-((4-methoxybenzyl)oxy)-4H-pyran-4-one
-
-
2-chloro-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-chloro-4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
-
2-Hydroxy acids
-
-
2-hydroxybenzoic acid
-
D-phenylglycine oxidation
2-Hydroxybutyric acid
-
D-phenylglycine oxidation
2-Ketobutyric acid
-
D-phenylglycine oxidation
2-oxo acids
-
-
2-oxobutyrate
-
D-alanine oxidation
2-phenyl-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
2-[(3,4-dichlorophenoxy)methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[(4-chlorophenoxy)methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[(4-fluorophenoxy)methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[(benzyloxy)methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[[(3,4-dichlorophenyl)sulfanyl]methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[[(4-chlorophenyl)sulfanyl]methyl]-5-hydroxy-4H-pyran-4-one
-
-
2-[[(4-fluorophenyl)sulfanyl]methyl]-5-hydroxy-4H-pyran-4-one
-
-
3,3-dibromo-4-chloro-5-fluoro-2,3-dihydro-1H-pyrrolo[2,3-b]pyridine
-
-
3-(3-chlorophenyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
3-(4-chlorophenyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
3-(hydroxymethyl)-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
3-Bromobenzoic acid
-
D-phenylglycine oxidation
3-chloro-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
3-chlorobenzoic acid
-
D-phenylglycine oxidation
3-cyano-4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
-
3-hydroxy-1,5-naphthyridin-2(1H)-one hydrobromide
-
-
3-hydroxy-1,6-naphthyridin-2(1H)-one hydrobromide
-
-
3-hydroxy-1,7-naphthyridin-2(1H)-one hydrobromide
-
-
3-hydroxy-1,8-naphthyridin-2(1H)-one
-
-
3-hydroxy-1,8-naphthyridin-2(1H)-one hydrobromide
-
-
3-hydroxy-4-methylquinolin-2(1H)-one
-
-
3-hydroxy-5-(2-phenylethyl)pyridin-2(1H)-one
-
-
-
3-hydroxy-5-methylpyridin-2(1H)-one
-
-
3-hydroxy-5-methylquinolin-2(1H)-one
-
-
3-hydroxy-6-methylquinolin-2(1H)-one
-
-
3-hydroxy-7-methylquinolin-2(1H)-one
-
-
3-hydroxy-8-methylquinolin-2(1H)-one
-
-
3-Hydroxybenzoic acid
-
D-phenylglycine oxidation
3-hydroxychromen-2-one
-
-
-
3-hydroxypyridin-2(1H)-one
-
-
3-hydroxyquinolin-2(1H)-one
-
-
3-hydroxyquinolin-2(1H)-one
-
-
3-hydroxyquinolin-2-(1H)-one
-
-
3-hydroxyquinoline-2-(1H)-one
-
very potent inhibitor
-
3-methylbenzoic acid
-
D-phenylglycine oxidation
3-Nitrobenzoic acid
-
D-phenylglycine oxidation
3-pyridin-3-yl-4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
4,5-dichlorofuran-2-carboxylic acid
-
-
4,6-difluoro-1-hydroxy-1H-benzo[d]imidazol-2(3H)-one
-
-
-
4,6-difuloro-1-hydroxy-1H-benzo[d]imidazol-2(3H)-one
-
-
-
4-(trifluoromethyl)-1,2-benzisoxazol-3-ol
-
-
4-chloro-5-fluoro-1H-pyrrolo[2,3-b]pyridine-2,3-dione
-
-
4-fluoro-1,2-benzisoxazol-3-ol
-
-
4-hydroxy-6-(1-phenylethyl)pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-(2-phenylethyl)pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-(3-phenylpropyl)pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-(phenoxymethyl)pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-[2-(3-methoxyphenyl)ethyl]pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-[2-(4-methoxyphenyl)ethyl]pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-[2-[2-(trifluoromethyl)phenyl]ethyl]pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-[2-[3-(trifluoromethyl)phenyl]ethyl]pyridazin-3(2H)-one
-
-
-
4-hydroxy-6-[2-[4-(trifluoromethyl)phenyl]ethyl]pyridazin-3(2H)-one
-
-
-
4-hydroxybenzoic acid
-
D-phenylglycine oxidation
4-[2-(4-chlorophenyl)ethyl]-1H-pyrrole-3-carboxylic acid
-
-
4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
-
4H-pyrrolo[2,3-d][1,3]oxazole-5-carboxylic acid
-
-
4H-pyrrolo[2,3-d][1,3]thiazole-5-carboxylic acid
-
-
4H-pyrrolo[3,2-d][1,3]thiazole-5-carboxylic acid
-
-
4H-thieno [3,2-b]pyrrole-5-carboxylic acid
-
i.e. compound 8, a moderately potent inhibitor of human DAAO in vitro
4H-thieno [3,2-b]pyrrole-5-carboxylic acid
-
i.e. compound 8, a moderately potent inhibitor of rat DAAO in vitro, inhibits DAAO activity in kideny by 96% and in brain by 80% resulting in a significant elevation in both plasma and cerebrospinal fluid D-serine concentration. Compound 8 fails to significantly influence amphetamine-induced psychomotor activity, nucleus accumbens dopamine release, or a dizocilpine maleate-induced deficit in novel object recognition in rats
4H-thieno[3,2-b] pyrrole-5-carboxylic acid
-
i.e. compound 8, very specific inhibitor of DAAO
4H-thieno[3,2-b] pyrrole-5-carboxylic acid
-
i.e. compound 8, very specific inhibitor of DAAO. Compound 8 at 200 mg/kg body weight inhibits rat kidney and cerebellar DAAO by approximately 96% and 80%, respectively. Effects after in vivo application, overview
4H-thieno[3,2-b]-pyrrole-5-carboxylic acid
-
-
4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
-
5,5'-dithiobis-nitrobenzoate
-
-
5-bromo-1,2-benzisoxazol-3-ol
-
-
5-chloro-3-hydroxypyridin-2(1H)-one
-
-
5-chloro-3-hydroxyquinolin-2(1H)-one
-
-
5-chloro-6-fluoro-3-hydroxy-1,8-naphthyridin-2(1H)-one
-
-
-
5-chloro-6-fluoro-3-hydroxy-1,8-naphthyridin-2(1H)-one hydrobromide
-
-
5-chloro-6-fluoro-3-hydroxyquinolin-2(1H)-one
-
-
5-chloro-benzo[d]isoxazol-3-ol
-
-
5-ethyl-3-hydroxyquinolin-2(1H)-one
-
-
5-fluoro-3-hydroxyquinolin-2(1H)-one
-
-
5-hydroxy-2-(2-phenylethyl)-4H-pyran-4-one
-
-
5-hydroxy-2-(methoxymethyl)-4H-pyran-4-one
-
-
5-hydroxy-2-(phenoxymethyl)-4H-pyran-4-one
-
-
5-hydroxy-2-[(naphthalen-1-yloxy)methyl]-4H-pyran-4-one
-
-
5-hydroxy-2-[(naphthalen-2-yloxy)methyl]-4H-pyran-4-one
-
-
5-hydroxy-2-[(phenylsulfanyl)methyl]-4H-pyran-4-one
-
-
5-hydroxy-2-[(pyridin-3-yloxy)methyl]-4H-pyran-4-one
-
-
5-hydroxy-2-[(pyrimidin-2-ylsulfanyl)methyl]-4H-pyran-4-one
-
-
5-iodo-1,2-benzisoxazol-3-ol
-
-
5-methyl-1H-pyrazole-3-carboxylic acid
-
-
5-Methylpyrazole-3-carboxylate
-
-
5-methylpyrazole-3-carboxylic acid
-
AS057278, selective D-amino acid oxidase inhibitor
5-methylpyrazole-3-carboxylic acid
-
AS057278, selective D-amino acid oxidase inhibitor, 10 mg/kg
5-methylpyrazole-3-carboxylic acid
-
i.e. AS057278
5-methylpyrazole-3-carboxylic acid
-
-
5-Methylthiophene-2-carboxylate
-
-
5-nitro-1,2-benzisoxazol-3-ol
-
-
6-(2-cyclohexylethyl)-4-hydroxypyridazin-3(2H)-one
-
-
-
6-(3,3-dimethylbutyl)-4-hydroxypyridazin-3(2H)-one
-
-
-
6-(trifluoromethyl)-1,2-benzisoxazol-3-ol
-
-
6-chloro-1,2-benzisoxazol-3-ol
-
-
6-chloro-3-hydroxyquinolin-2(1H)-one
-
-
6-chlorobenzo(d)isoxazol-3-ol
-
-
-
6-chlorobenzo[d]isoxazol-3-ol
-
potent, competitive inhibitor
6-chlorobenzo[d]isoxazol-3-ol
-
competitive
6-chlorobenzo[d]isoxazol-3-ol
-
-
6-chlorobenzo[d]isoxazole-3-ol
-
-
-
6-cyclohexyl-4-hydroxypyridazin-3(2H)-one
-
-
-
6-ethoxy-1,2-benzisoxazol-3-ol
-
-
6-fluoro-1,2-benzisoxazol-3-ol
-
-
6-fluoro-3-hydroxyquinolin-2(1H)-one
-
-
6-methoxy-1,2-benzisoxazol-3-ol
-
-
6-methyl-1,2-benzisoxazol-3-ol
-
-
6-nitro-1,2-benzisoxazol-3-ol
-
-
6-[(E)-2-(4-chlorophenyl)vinyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(2-fluorophenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(3,5-difluorophenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(3,5-dimethoxyphenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(3-fluorophenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(4-chlorophenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-(4-fluorophenyl)ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6-[2-[3,5-bis(trifluoromethyl)phenyl]ethyl]-4-hydroxypyridazin-3(2H)-one
-
-
-
6H-thieno[2,3-b]pyrrole-5-carboxylic acid
-
-
7-(5-oxoadipoamido)cephalosporanic acid
-
product inhibition
7-chloro-3-hydroxyquinolin-2(1H)-one
-
-
7-ethyl-3-hydroxyquinolin-2(1H)-one
-
-
7-fluoro-1,2-benzisoxazol-3-ol
-
-
7-fluoro-3-hydroxyquinolin-2(1H)-one
-
-
7-fluoro-6-(trifluoromethyl)-1,2-benzisoxazol-3-ol
-
-
7-hydroxypyrido[2,3-b]pyrazin-6(5H)-one
-
-
7-methyl-1,2-benzisoxazol-3-ol
-
-
8-chloro-3-hydroxyquinolin-2(1H)-one
-
-
8-ethyl-3-hydroxyquinolin-2(1H)-one
-
-
8-fluoro-3-hydroxyquinolin-2(1H)-one
-
-
acetaldehyde
-
-
Acetylsalicylate
-
-
adenosine 5'-monophosphate
-
-
ADP
-
-
ADPribose
-
-
aniline
-
slight, D-alanine oxidation
aniline
-
-
anthranilate
-
-
anthranilate
-
-
anthranilate
-
-
Benzoate
-
75 mM, 76% inhibition
Benzoate
-
competitive
Benzoate
-
potent competitive inhibitor
Benzoate
-
-
Benzoate
-
-
Benzoate
-
a classical substrate-competitive inhibitor, effect of benzoate on the FAD-binding process to hDAAO apoprotein
Benzoate
-
competitive inhibitor
Benzoate
-
moderate inhibition (49.7% residual activity at 1 mM)
Benzoate
-
-
Benzoic acid
-
D-phenylglycine oxidation
Benzoic acid
-
not
Benzoic acid
-
-
Benzoic acid
-
competitive inhibitor
benzo[d]isoxazol-3-ol
-
-
beta-Naphthoflavone
-
-
Ca2+
-
83.1% residual activity at 10 mM
Chloramphenicol
-
-
chlorpromazine
-
-
chlorpromazine
-
a non-specific DAO inhibitor
chlorpromazine
-
competitive versus cofactor FAD
chlorpromazine
-
-
chlorpromazine
-
FAD-competitive inhibitor
chlortetracycline
-
-
creatinine
-
75 mM, 86% inhibition
crotonate
-
-
crotonic acid
-
D-phenylglycine oxidation
cysteine
-
-
D-2-Hydroxy-3-methylvalerate
-
D-alanine oxidation
D-lactate
-
slight, D-alanine oxidation
D-serine
-
-
dCMP
-
-
DELTA1-Piperidine 2-carboxylate
-
D-lysine oxidation
dephospho-CoA
-
-
DL-2-Hydroxybutyrate
-
D-alanine oxidation
DL-2-Hydroxyoctanoate
-
D-alanine oxidation
Ethacrynic acid
-
-
Flufenamic acid
-
-
formaldehyde
-
-
Furosemide
-
-
glutaryl-7-aminocephalosporanic acid
-
noncompetitive
glutaryl-7-aminocephalosporanic acid
-
-
glutaryl-7-aminocephalosporanic acid
-
noncompetitive
glutaryl-7-aminocephalosporanic acid
-
-
Glycyl-DL-norvaline
-
slight, D-alanine oxidation
glycyl-DL-phenylalanine
-
slight, D-alanine oxidation
Glycyl-DL-tryptophan
-
slight, D-alanine oxidation
Glycyl-DL-valine
-
slight, D-alanine oxidation
H2O2
-
-
H2O2
-
product inhibition, competitive versus cephalosporin
H2O2
-
product inhibition, noncompetitive versus cephalosporin
H2O2
-
product inhibition, competitive versus cephalosporin
Hg2+
-
1 mM
HgCl2
-
0.1 mM, 71% inhibition
Histidyl-histidine
-
slight, D-alanine oxidation
indomethacin
-
-
iodoacetamide
-
1 mM
iodoacetamide
-
89.8% residual activity at 1 mM
iodoacetate
-
81.6% residual activity at 1 mM
KCl
-
about 30% residual activity at 250 mM KCl
kojic acid
-
D-phenylglycine oxidation
kojic acid
-
-
L-histidine
-
D-histidine oxidation
L-leucine
-
D-alanine oxidation
L-methionine
-
D-alanine oxidation
L-methionine
-
slightly inhibited in the presence of L-methionine
L-norvaline
-
D-alanine oxidation
malate
-
78.6% residual activity at 10 mM
malonate
-
-
Mefenamic acid
-
-
Mersalyl
-
-
more homo
-
pLG72 binds the enzyme and negatively affects its enzymatic activity
-
NaCl
-
direct inhibition by NaCl is due to FAD dissociation
NADH
-
-
NADPH
-
-
nicotinate
-
-
nicotinic acid
-
D-phenylglycine oxidation
p-Aminosalicylic acid
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
0.1 mM
p-chloromercuribenzoate
-
moderate inhibition (61.2% residual activity at 1 mM)
p-hydroxymercuribenzoate
-
significant inhibition (6.2% residual activity at 1 mM)
PCMB
-
75 mM, 93% inhibition
Penicillamine
-
-
penicillin
-
-
Phenylbutazone
-
-
Phenylmercuriacetate
-
-
Picolinic acid
-
D-phenylglycine oxidation
pLG72
-
acts as a negative effector of DAAO
-
pLG72
-
activity decreases in the presence of pLG72 (0.27 mg/ml)
-
poly(methylene-co-guanidine)
-
in the presence of poly(methylene-co-guanidine) (1.8%, w/v) the enzyme activity decreases irreversibly with a half-life time of about 1.75 min at 25C
-
pyrrole-2-carboxylate
-
-
Pyrrolidine
-
slight, D-alanine oxidation
pyruvate
-
slight, D-alanine oxidation
riboflavin 5'-monophosphate
-
-
salicylate
-
-
Silver nitrate
-
0.1 mM, 66% inhibition
Sodium benzoate
-
-
Sodium benzoate
-
inhibits the enzyme activity by by impeding the interaction of the enzyme with the flavin prosthetic group
Sodium benzoate
-
inhibits the enzyme activity by impeding the interaction of the enzyme with the flavin prosthetic group
Sodium benzoate
-
competitive inhibitor, inhibits the development of mechanical allodynia, caused by enhanced enzyme expression, in the spinal cord, overview
Straight-chain fatty acids
-
-
-
streptomycin
-
-
succinate
-
90.7% residual activity at 1 mM
succinate semialdehyde
-
-
Tartrate
-
79.5% residual activity at 10 mM
trans-2-Pentenoic acid
-
D-phenylglycine oxidation
trifluoromethyl-D-alanine
-
pseudo-substrate
-
tropolone
-
-
UMP
-
-
Zn2+
-
44% residual activity at 10 mM
Mg2+
-
84.9% residual activity at 10 mM
additional information
-
resistant to the oxidants O2 and H2O2
-
additional information
-
bovine serum albumin does not affect DAAO activity
-
additional information
-
negligible DAAO inhibition with muscimol, (S)-AMPA, 6-chloro-3-methoxybenzo[d]isoxazole, and 4,5,6,7-tetrahydrobenzo[d]isoxazol-3-ol
-
additional information
-
oxidative modification of Trigonopsis variabilis D-amino acid oxidase in vivo is traceable as the conversion of Cys108 into a stable cysteine sulfinic acid, causes substantial loss of activity and thermostability of the enzyme
-
additional information
-
benzoate and CPZ similarly modify the short-term cellular D-serine concentration but affect the cellular concentration of hDAAO differently
-
additional information
-
not inhibited by sodium azide (0.8 M, 30 min)
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine
-
MK-801, a dose-dependent elevation in the expression of DAO mRNA is observed in most brain areas 4 h after the administration (1.6 mg/kg)
D-amino acid oxidase activator
-
i.e. DAOA or G72, the gene encoding the activator is located on chromosome 13q32-34
-
DAO activator
-
DAOA, formerly G72
-
morphine
-
the chronic treatment with morphine produces a slight but significant enhancement in the levels of DAO mRNA in the forebrain regions, the levels increases by 24%-102% in the striatum (87% increase), hippocampus (102%), and cortex (24%)
pLG72
-
i.e. DAOA or D-amino acid oxidase activator, interacts with and activates the enzyme, genotyping in healthy persons and schizophrenia patients, overview
-
pLG72
-
i.e. DAOA or D-amino acid oxidase activator, interacts with and activates the enzyme
-
G72 protein
-
-
-
additional information
-
exogenous FAD (0.01 to 1 mM) added to assay or protein storage buffers does not enhance the catalytic activity of native and recombinant DAO
-
additional information
-
activity of the DAAO after incubation in water-insoluble ionic liquids is higher than in water-soluble ones
-
additional information
-
the activity in the crude extract is not increased when external FAD is added
-
additional information
-
no significant change in the protein expression of DAO is observed after administration of morphine for 30 days in the diencephalon, midbrain, pons medulla, and cerebellum
-
additional information
-
mean DAAOactivity is 2fold higher in the schizophrenia patients group compared with the control group
-
additional information
-
L5/L6 spinal nerve ligation in the lumbar spinal cord increases the enzyme activity with an increase in neuropathic pain, mechanical allodynia
-
additional information
-
the enzyme activity is increased in schizophrenia
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.015
2,6-dichlorophenolindophenol
-
with D-alanine
0.83
cephalosporin C
-
-
0.9
cephalosporin C
-
recombinant mutant F54S, pH 7.5, 22C
1
cephalosporin C
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
1.2
cephalosporin C
-
recombinant mutant F54A, pH 7.5, 22C
1.6
cephalosporin C
-
recombinant wild-type enzyme, pH 7.5, 22C
1.7
cephalosporin C
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
1.9
cephalosporin C
-
pH 8.5, 25C, mutant enzyme Y238F; pH 8.5, 25C, mutant enzyme Y238S
1.9
cephalosporin C
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.3
cephalosporin C
-
pH 8.5, 25C
2.4
cephalosporin C
-
pH 8.5, 25C
2.5
cephalosporin C
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.8
cephalosporin C
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3.8
cephalosporin C
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
4
cephalosporin C
-
pH 8.5, 25C
4.3
cephalosporin C
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
4.8
cephalosporin C
-
recombinant mutant F54Y, pH 7.5, 22C
5
cephalosporin C
-
pH 8.5, 25C, wild-type enzyme
5
cephalosporin C
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
5
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
6
cephalosporin C
-
native enzyme, at 37C
6
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
6
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
8.7
cephalosporin C
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
9
cephalosporin C
-
native enzyme, at 37C
9
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
13
cephalosporin C
-
-
13
cephalosporin C
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
13
cephalosporin C
-
double fusion enzyme, at 37C
20
cephalosporin C
-
double fusion enzyme, at 37C
23
cephalosporin C
-
-
28
cephalosporin C
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6.7
citrulline
-
-
0.03
D-1-naphthyl-alanine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.04
D-1-naphthyl-alanine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.05
D-1-naphthyl-glycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.33
D-1-naphthyl-glycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.46
D-2-Aminobutyrate
-
-
0.13
D-2-chloro-phenylglycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.4
D-2-chloro-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.5
D-2-fluoro-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C; wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.04
D-2-naphthyl-alanine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.06
D-2-naphthyl-alanine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.01
D-2-naphthyl-glycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.03
D-2-naphthyl-glycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.03
D-4-chloro-phenylglycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.2
D-4-chloro-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.6
D-4-fluoro-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.7
D-4-fluoro-phenylglycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
1.6
D-4-hydroxy-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
2.1
D-4-hydroxy-phenylglycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.23
D-Ala
-
pH 8.0, 37C
0.77
D-Ala
-
wild-type enzyme
0.8
D-Ala
-
pH 8.5, 25C, wild-type enzyme
0.8
D-Ala
-
pH 8.5, 25C, wild-type enzyme, at
0.9
D-Ala
-
pH 8.3, 25C
1
D-Ala
-
pH 8.5, 25C
1.1
D-Ala
-
pH 8.5, 25C, mutant enzyme S335G
1.2
D-Ala
-
pH 8.3, 37C, mutant enzyme F42C
1.3
D-Ala
-
pH 8.5, 25C
1.5
D-Ala
-
pH 7.5, 25C, mutant enzyme DELTASer308-Lys321
1.7
D-Ala
-
pH 8.5, 25C
2.5
D-Ala
-
pH 8.3, 37C, wild-type enzyme
2.6
D-Ala
-
pH 8.5, 25C, wild-type enzyme
6.89
D-Ala
-
pH 8.3, 37C, recombinant DAO
7
D-Ala
-
pH 8.5, 25C
7.5
D-Ala
-
pH 8.5, 25C, mutant enzyme Y238F
7.8
D-Ala
-
pH 8.5, 25C, mutant enzyme Y238S
16.7
D-Ala
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
17.8
D-Ala
-
pH 8.5, 25C, mutant enzyme M213R
44
D-Ala
-
mutant enzyme E220D/Y224G
45.8
D-Ala
-
pH 8.5, 25C, mutant enzyme M213R/Y238R
46
D-Ala
-
mutant enzyme R221D/Y224G
0.036
D-alanine
-
pH 8.5, 15C, mutant G52V
0.2
D-alanine
-
apparent value, at 25C
0.22
D-alanine
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.4
D-alanine
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.5
D-alanine
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.6
D-alanine
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.6
D-alanine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25C and pH 8.5
0.7
D-alanine
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.7
D-alanine
-
free enzyme
0.8
D-alanine
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.83
D-alanine
-
-
0.9
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25C
0.9
D-alanine
-
wild type enzyme, apparent value, at 25C and pH 8.5
1
D-alanine
-
mutant T317A
1
D-alanine
-
wild-type
1
D-alanine
-
apparent value, at 25C
1
D-alanine
-
immobilized enzyme
1.1
D-alanine
-
wild-type
1.1
D-alanine
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
1.29
D-alanine
-
38.3 kDa form
1.3
D-alanine
-
mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
1.3
D-alanine
-
apparent value, at 25C
1.3
D-alanine
-
at pH 7.0 and 25C
1.4
D-alanine
-
mutant enzyme W243Y
1.6
D-alanine
-
mutant enzyme W243I, assay in presence of a large excess of free FAD (0.2 mM) in assay mixture
1.6
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25C
1.7
D-alanine
-
apparent value, at 25C
1.8
D-alanine
-
-
1.8
D-alanine
-
pH 8.5, 15C, mutant Q144R
2
D-alanine
-
native enzyme, at 37C
2
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C; immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
2
D-alanine
-
at pH 8.3 and 25C
2.3
D-alanine
-
free enzyme
2.3
D-alanine
-
wild-type
2.6
D-alanine
-
wild-type
2.6
D-alanine
P80340
at pH 8.3 and 25C
2.6
D-alanine
-
pH 8.5, 15C, wild-type enzyme
2.8
D-alanine
-
mutant Y224F
3
D-alanine
-
native enzyme, at 37C
3
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
4
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
4.28
D-alanine
-
-
4.3
D-alanine
-
apparent value, at 25C
4.5
D-alanine
-
at pH 8.3 and 25C
4.63
D-alanine
-
wild-type
4.7
D-alanine
-
pH 8.5, 15C, mutant S19G/S120P/Q144R/K321M/A345V
4.9
D-alanine
-
mutant enzyme M213G, apparent value, at 25C and pH 8.5
5.5
D-alanine
-
apparent value, reconstituted holoenzyme at pH 8.0 and 30C
6
D-alanine
-
double fusion enzyme, at 37C
6.5
D-alanine
-
-
7
D-alanine
-
apparent value, at 25C
8
D-alanine
-
mutant G281C
8.8
D-alanine
-
at pH 8.3 and 25C
9.36
D-alanine
-
mutant D206E
13
D-alanine
-
FAD-S mutant enzyme
13
D-alanine
-
double fusion enzyme, at 37C
18
D-alanine
-
-
18
D-alanine
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
18.12
D-alanine
-
mutant D206A
18.5
D-alanine
-
mutant enzyme M213R, apparent value, at 25C and pH 8.5
20.52
D-alanine
-
mutant D206S
27
D-alanine
-
mutant Y228F
27.4
D-alanine
-
-
40
D-alanine
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
43
D-alanine
-
-
76
D-alanine
-
-
140
D-alanine
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
310
D-alanine
-
mutant R285A
800
D-alanine
-
mutant R285K
1.01
D-Arg
-
pH 8.3, 37C, recombinant DAO
3
D-Arg
-
mutant enzyme E220D/Y224G; mutant enzyme R221D/Y224G
13
D-Arg
-
wild-type enzyme
1.3
D-Arginine
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2 - 3
D-Arginine
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.5
D-Arginine
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
5.5
D-Arginine
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6.7
D-Arginine
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
9.2
D-Arginine
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
9.7
D-Arginine
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
13
D-Arginine
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
18
D-Arginine
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
29
D-Arginine
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
11
D-Asn
-
pH 8.5, 25C, mutant enzyme M213R
14.4
D-Asn
-
pH 8.5, 25C, wild-type enzyme
22
D-Asn
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
22.6
D-Asn
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
29
D-Asn
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
50
D-Asn
-
Km above 50 mM, mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
2
D-Asp
-
pH 8.5, 25C, mutant enzyme M213R
18
D-Asp
-
pH 8.5, 25C, wild-type enzyme
28
D-Asp
-
pH 8.5, 25C, mutant enzyme M213R/Y238R
30
D-Asp
-
pH 8.5, 25C, mutant enzyme S335G
2000
D-Asp
-
pH 8.5, 25C
2
D-Aspartate
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
5.2
D-Aspartate
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
15
D-Aspartate
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
17
D-Aspartate
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
18
D-Aspartate
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
30
D-Aspartate
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
33
D-Aspartate
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
53
D-Aspartate
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
61
D-Aspartate
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
72
D-Aspartate
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
98
D-Aspartate
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
150
D-Aspartate
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2000
D-Aspartate
-
at pH 7.0 and 25C
21.41
D-Cysteine
-
-
1.5
D-Dopa
-
in 50 mM sodium diphosphate buffer (pH 8.3) at 25C
1.5
D-Dopa
-
at pH 7.0 and 25C
3.8
D-Gln
-
pH 8.5, 25C, mutant enzyme M213R
4.6
D-Gln
-
pH 8.5, 25C, wild-type enzyme
33
D-Glu
-
pH 8.5, 25C, mutant enzyme M213R
77.3
D-Glu
-
pH 8.5, 25C, wild-type enzyme
5.1
D-glutamate
-
mutant enzyme M213G, apparent value, at 25C and pH 8.5
21.1
D-glutamate
-
mutant enzyme M213R, apparent value, at 25C and pH 8.5
76
D-glutamate
-
wild type enzyme, apparent value, at 25C and pH 8.5
101
D-glutamate
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25C and pH 8.5
2.5
D-glutamine
-
mutant enzyme M213G, apparent value, at 25C and pH 8.5
5.4
D-glutamine
-
mutant enzyme M213R, apparent value, at 25C and pH 8.5; wild type enzyme, apparent value, at 25C and pH 8.5
6
D-glutamine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25C and pH 8.5
2.89
D-His
-
pH 8.3, 37C, recombinant DAO
0.15
D-homo-phenylalanine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.3
D-homo-phenylalanine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
1.06
D-Ile
-
pH 8.0, 37C
0.227
D-Leu
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.78
D-Leu
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
1.19
D-Leu
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
1.81
D-Leu
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
6.5
D-Leu
-
pH 8.0, 37C
0.08
D-Leucine
-
-
0.23
D-Leucine
-
-
0.28
D-Leucine
-
-
0.28
D-Leucine
-
-
0.5
D-Leucine
-
-
10.9
D-Leucine
-
-
0.425
D-Lys
-
pH 8.0, 37C
10
D-Lys
-
wild-type enzyme
14
D-Lys
-
mutant enzyme E220D/Y224G
20
D-Lys
-
mutant enzyme R221D/Y224G
29.3
D-Lys
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
8
D-Lysine
-
mutant enzyme M213G, apparent value, at 25C and pH 8.5
14.6
D-Lysine
-
wild type enzyme, apparent value, at 25C and pH 8.5
16.6
D-Lysine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25C and pH 8.5
0.2
D-Met
-
pH 8.5, 25C, wild-type enzyme
0.4
D-Met
-
pH 8.3, 37C, mutant enzyme F42C
0.46
D-Met
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.65
D-Met
-
wild-type enzyme
0.67
D-Met
-
mutant enzyme E220D/Y224G; mutant enzyme R221D/Y224G
0.9
D-Met
-
pH 8.3, 37C, wild-type enzyme
1.5
D-Met
-
pH 8.5, 25C, mutant enzyme M213R
2.9
D-Met
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
3.3
D-Met
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
4.2
D-Met
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.24
D-methionine
-
-
0.27
D-methionine
-
-
0.29
D-methionine
-
-
0.3
D-methionine
-
mutant enzyme T60A/Q144R/K152E, apparent value, at 25C and pH 8.5
0.6
D-methionine
-
wild type enzyme, apparent value, at 25C and pH 8.5
0.76
D-methionine
-
-
0.8
D-methionine
-
mutant enzyme M213G, apparent value, at 25C and pH 8.5
0.81
D-methionine
-
-
0.83
D-methionine
-
-
0.83
D-methionine
-
-
1.2
D-methionine
-
mutant enzyme M213R, apparent value, at 25C and pH 8.5
15.6
D-methionine
-
mutant Y224F
27.4
D-methionine
-
-
50
D-methionine
-
mutant Y228F
143
D-methionine
-
wild-type
0.39
D-Norleucine
-
-
0.4
D-Norleucine
-
-
0.063
D-Norvaline
-
-
0.89
D-Norvaline
-
-
0.04
D-Phe
-
pH 8.5, 25C, mutant enzyme Y238F
0.068
D-Phe
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.07
D-Phe
-
pH 8.5, 25C, mutant enzyme Y238S
0.134
D-Phe
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.3
D-Phe
-
pH 8.5, 25C, wild-type enzyme
0.357
D-Phe
-
pH 8.0, 37C
0.37
D-Phe
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.77
D-Phe
-
mutant enzyme R221D/Y224G
1.4
D-Phe
-
mutant enzyme E220D/Y224G; wild-type enzyme
1.68
D-Phe
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
1.9
D-Phe
-
pH 8.3, 37C, mutant enzyme F42C
2.72
D-Phe
-
pH 8.3, 37C, recombinant DAO
3.6
D-Phe
-
pH 8.3, 37C, wild-type enzyme
0.2
D-phenylalanine
-
-
0.2
D-phenylalanine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
0.29
D-phenylalanine
-
-
0.3
D-phenylalanine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
1.2
D-phenylalanine
-
in 50 mM sodium diphosphate buffer (pH 8.3) at 25C
1.38
D-phenylalanine
-
pH 8.0
1.4
D-phenylalanine
-
wild-type
1.5
D-phenylalanine
-
-
2.7
D-phenylalanine
-
at pH 7.0 and 25C
7.5
D-phenylalanine
-
mutant Y224F
9.1
D-phenylalanine
-
mutant T317A
10
D-phenylalanine
-
-
18.3
D-phenylalanine
-
mutant Y228F
35
D-phenylalanine
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
55.9
D-phenylalanine
-
wild-type
0.38
D-phenylglycine
-
-
3.5
D-phenylglycine
-
wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
5.2
D-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25C
7
D-phenylglycine
-
-
0.56
D-Pro
-
wild-type enzyme
1.13
D-Pro
-
pH 8.0, 37C
1.7
D-Pro
-
pH 8.3, 25C
4.1
D-Pro
-
mutant enzyme E220D/Y224G
7.7
D-Pro
-
mutant enzyme R221D/Y224G
8.5
D-Pro
-
pH 8.5, 25C
12.3
D-Pro
-
pH 8.5, 25C, mutant enzyme Y238S
13.5
D-Pro
-
pH 8.5, 25C, mutant enzyme Y238F
21.5
D-Pro
-
pH 8.5, 25C, wild-type enzyme
1280
D-Pro
-
pH 8.5, 25C, mutant enzyme M213R
1.1
D-proline
-
wild-type
1.2
D-proline
-
mutant T317A
1.7
D-proline
-
-
5.6
D-proline
-
immobilized wild type enyzme, at 25C
8.5
D-proline
-
at pH 7.0 and 25C
9.1
D-proline
-
mutant Y224F
9.5
D-proline
-
wild-type
12.2
D-proline
-
mutant Y228F
12.5
D-proline
-
free wild type enyzme, at 25C
15.5
D-proline
-
38.3 kDa form
21.47
D-proline
-
-
21.5
D-proline
-
-
86
D-proline
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
0.909
D-Ser
-
pH 8.0, 37C
1.7
D-Ser
-
pH 8.5, 25C, mutant enzyme Y238F
2.9
D-Ser
-
pH 8.5, 25C, mutant enzyme Y238S
3.3
D-Ser
-
wild-type enzyme
3.6
D-Ser
-
pH 8.3, 25C
3.62
D-Ser
-
apparent value, immobilized enzyme
4.7
D-Ser
-
pH 8.3, 37C, mutant enzyme F42C
7.5
D-Ser
-
pH 8.5, 25C
12.7
D-Ser
-
pH 8.3, 37C, wild-type enzyme
13.7
D-Ser
-
pH 8.5, 25C, wild-type enzyme
13.75
D-Ser
-
apparent value, free enzyme
20
D-Ser
-
pH 8.3, 37C, recombinant DAO
36.6
D-Ser
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
3.9
D-serine
-
in 50 mM sodium diphosphate buffer (pH 8.3) at 25C
4
D-serine
-
pH 8.5, 25C, recombinant EGFP-DAAO
4.8
D-serine
-
wild-type
7
D-serine
-
pH 8.5, 25C, recombinant wild-type DAAO
7.5
D-serine
-
at pH 7.0 and 25C
13.75
D-serine
-
-
25
D-serine
-
-
33.7
D-serine
-
-
310
D-serine
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
11.1
D-Thr
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.2
D-Trp
-
pH 8.5, 25C, mutant enzyme Y238S
0.3
D-Trp
-
pH 8.5, 25C, mutant enzyme Y238F; pH 8.5, 25C, wild-type enzyme
0.4
D-Trp
-
pH 7.5, 25C, mutant enzyme DELTASer308-Lys321
0.45
D-Trp
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.46
D-Trp
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.49
D-Trp
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
2.7
D-Trp
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.17
D-tryptophan
-
-
0.3
D-tryptophan
-
-
0.7
D-tryptophan
-
immobilized enzyme
1.2
D-tryptophan
-
free enzyme
1.2
D-tryptophan
-
-
1.4
D-tryptophan
-
-
1.5
D-tryptophan
-
at pH 7.0 and 25C
15
D-tryptophan
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
0.087
D-Tyr
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.226
D-Tyr
-
pH 8.0, 37C
0.45
D-Tyr
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.84
D-Tyr
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
1.1
D-tyrosine
-
in 50 mM sodium diphosphate buffer (pH 8.3) at 25C
1.5
D-tyrosine
-
at pH 7.0 and 25C
0.263
D-Val
-
pH 8.0, 37C
0.63
D-Val
-
wild-type enzyme
3.1
D-Val
-
mutant enzyme E220D/Y224G
3.7
D-Val
-
mutant enzyme R221D/Y224G
6
D-Val
-
pH 8.5, 25C, mutant enzyme Y238F
6.1
D-Val
-
pH 8.5, 25C, mutant enzyme Y238S
14.4
D-Val
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
18.9
D-Val
-
pH 8.5, 25C, wild-type enzyme
41
D-Val
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
46
D-Val
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
0.67
D-valine
-
wild-type
1.3
D-valine
-
immobilized wild type enyzme, at 25C
1.4
D-valine
-
-
3.5
D-valine
-
free enzyme
3.95
D-valine
-
-
4.3
D-valine
-
mutant T317A
18.89
D-valine
-
-
18.9
D-valine
-
free wild type enyzme, at 25C
20
D-valine
-
-
1
Ethionine
-
-
0.00045
FAD
-
-
140
Gly
-
pH 8.3, 25C
180
Gly
-
pH 8.5, 25C
180
glycine
-
at pH 7.0 and 25C
18
N-Methyl-D-aspartate
-
pH 8.5, 25C, mutant enzyme M213R
0.06
O2
-
mutant R285K
0.08
O2
-
mutant R285A
0.15
O2
-
at pH 8.3 and 25C
0.15
O2
-
pH 8.5, 15C, mutant G52V
0.18
O2
-
with D-alanine
0.18
O2
-
with D-methionine
0.18
O2
-
-
0.25
O2
-
pH 8.5, 25C, mutant enzyme S335G, reaction with D-Ala
0.26
O2
-
pH 8.5, 25C, mutant enzyme Y238F
0.5
O2
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
0.8
O2
-
at pH 8.3 and 25C
0.95
O2
-
cephalosporin C
0.96
O2
-
pH 8.5, 25C, mutant enzyme Y238S
1.02
O2
-
D-alanine
1.1
O2
-
-
1.2
O2
-
at pH 8.3 and 25C
1.4
O2
-
pH 8.5, 15C, mutant Q144R
2.3
O2
-
wild-type
2.3
O2
-
pH 8.5, 25C, wild-type enzyme, reaction with D-Ala
2.3
O2
-
pH 8.5, 25C, wild-type enzyme
2.3
O2
P80340
at pH 8.3 and 25C
3
O2
-
pH 8.5, 15C, wild-type enzyme
5
O2
-
pH 8.5, 15C, wild-type enzyme
6.1
O2
-
pH 8.5, 15C, mutant S19G/S120P/Q144R/K321M/A345V
0.06
phenylalanine
-
-
17.3
serine
-
-
20.8
serine
-
mutant T317A
1.99
thiazolidine-2-carboxylate
-
-
5.8
tyrosine
-
-
0.067
methylene blue
-
with D-alanine
additional information
additional information
-
the dextran-conjugated enzyme shows decreased KM-values for D-amino acids
-
additional information
additional information
-
steady-state kinetics, reductive and oxidative half-reactions, overview
-
additional information
additional information
-
kinetics in comparison to the human enzyme, overview
-
additional information
additional information
-
reaction kinetics of oxidative and reductive half-reactions of wild-type and mutant enzymes, overview
-
additional information
additional information
-
DAAO-lingand interaction kinetics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.3
cephalosporin C
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3 - 6
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
3.6
cephalosporin C
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
4.4
cephalosporin C
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6.167
cephalosporin C
-
recombinant wild-type enzyme, pH 7.5, 22C
7
cephalosporin C
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
7.3
cephalosporin C
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
7.8
cephalosporin C
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
15
cephalosporin C
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
16.67
cephalosporin C
-
recombinant mutant F54S, pH 7.5, 22C
17
cephalosporin C
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
18.33
cephalosporin C
-
recombinant mutant F54A, pH 7.5, 22C
24
cephalosporin C
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
26
cephalosporin C
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
36.67
cephalosporin C
-
recombinant mutant F54Y, pH 7.5, 22C
39
cephalosporin C
-
pH 8.5, 25C
50
cephalosporin C
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
55
cephalosporin C
-
native enzyme, at 37C
55
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
57
cephalosporin C
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
64
cephalosporin C
-
double fusion enzyme, at 37C
70
cephalosporin C
-
native enzyme, at 37C
70
cephalosporin C
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
72
cephalosporin C
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
80
cephalosporin C
-
double fusion enzyme, at 37C
100
cephalosporin C
-
-
737
cephalosporin C
-
-
4300
cephalosporin C
-
pH 8.5, 25C
4400
cephalosporin C
-
pH 8.5, 25C
0.75
D-Ala
-
mutant enzyme R221D/Y224G
3
D-Ala
-
mutant enzyme E220D/Y224G
5.2
D-Ala
-
pH 8.5, 25C
5.5
D-Ala
-
pH 8.3, 25C
6.5
D-Ala
-
wild-type enzyme
14.9
D-Ala
-
pH 8.3, 37C, recombinant DAO
82
D-Ala
-
pH 8.5, 25C, mutant enzyme S335G
108.6
D-Ala
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
120
D-Ala
-
pH 8.5, 25C, mutant enzyme Y238S
125
D-Ala
-
pH 8.5, 25C, mutant enzyme Y238F
190
D-Ala
-
pH 8.0, 37C
278
D-Ala
-
pH 8.5, 25C, mutant enzyme M213R
350
D-Ala
-
pH 8.5, 25C, wild-type enzyme
440
D-Ala
-
pH 8.5, 25C
630
D-Ala
-
pH 8.5, 25C, mutant enzyme M213R
2740
D-Ala
-
pH 8.5, 25C
4880
D-Ala
-
pH 8.5, 25C
5000
D-Ala
-
pH 8.5, 25C, wild-type enzyme
0.000167
D-alanine
-
mutant D206A
0.001
D-alanine
-
mutant D206S
0.00233
D-alanine
-
mutant D206E
0.00567
D-alanine
-
wild-type
0.05
D-alanine
-
mutant R285A
0.05
D-alanine
-
-
0.33
D-alanine
-
pH 8.5, 15C, mutant G52V
0.63
D-alanine
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.79
D-alanine
-
mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.8
D-alanine
-
mutant R285K
0.8
D-alanine
-
-
0.92
D-alanine
-
mutant T317A
1.5
D-alanine
-
mutant G281C
2.6
D-alanine
-
FAD-S mutant enzyme
3.7
D-alanine
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
4.2
D-alanine
-
mutant Y223S
5
D-alanine
-
mutant Y228F
5.2
D-alanine
-
apparent value, at 25C
5.2
D-alanine
-
at pH 7.0 and 25C
6.9
D-alanine
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
7.3
D-alanine
-
apparent value, at 25C
7.33
D-alanine
-
D isotope
8.5
D-alanine
-
wild-type
9.2
D-alanine
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
10
D-alanine
-
wild-type
10
D-alanine
-
-
10
D-alanine
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
10
D-alanine
-
at pH 8.3 and 25C
11.6
D-alanine
-
mutant Y224F
12.7
D-alanine
-
wild-type
13.8
D-alanine
-
immobilized enzyme
14.7
D-alanine
-
at pH 8.3 and 25C
15
D-alanine
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
23.3
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25C
25
D-alanine
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
27
D-alanine
-
in 75 mM sodium diphosphate buffer (pH 8.3) at 25C
33.8
D-alanine
-
free enzyme
44
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
46
D-alanine
-
apparent value, at 25C
48
D-alanine
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
51
D-alanine
-
immobilized enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
52.4
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25C
52.5
D-alanine
-
-
53
D-alanine
-
at pH 8.3 and 25C
60
D-alanine
-
native enzyme, at 37C
60
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
67
D-alanine
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
70.8
D-alanine
-
D isotope
74
D-alanine
-
native enzyme, at 37C
74
D-alanine
-
free enzyme, in 100 mM potassium phosphate buffer, pH 8.0, at 25C
75
D-alanine
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
78
D-alanine
-
double fusion enzyme, at 37C
80
D-alanine
-
apparent value, at 25C
81
D-alanine
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
85
D-alanine
-
apparent value, at 25C
88
D-alanine
-
double fusion enzyme, at 37C
95
D-alanine
-
apparent value, reconstituted holoenzyme at pH 8.0 and 30C
95
D-alanine
-
apparent value, at 25C
140
D-alanine
-
pH 8.5, 15C, mutant Q144R
210
D-alanine
-
mutant Y223F
300
D-alanine
-
-
330
D-alanine
-
pH 8.5, 15C, wild-type enzyme
350
D-alanine
-
wild-type
350
D-alanine
-
-
350
D-alanine
P80340
at pH 8.3 and 25C
370
D-alanine
-
pH 8.5, 15C, mutant S19G/S120P/Q144R/K321M/A345V
721
D-alanine
-
-
1.83
D-Arg
-
mutant enzyme I223D/Y224G
2
D-Arg
-
mutant enzymeY224G
2.83
D-Arg
-
pH 8.3, 37C, recombinant DAO
3.5
D-Arg
-
wild-type enzyme
4
D-Arg
-
mutant enzyme E220D/Y224G; mutant enzyme G222D/Y224G
7.5
D-Arg
-
mutant enzyme R221D/Y224G
0.2
D-Arginine
-
kcat less than 0.2/s, mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.43
D-Arginine
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.1
D-Arginine
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.6
D-Arginine
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3.2
D-Arginine
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3.6
D-Arginine
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6.5
D-Arginine
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
7.3
D-Arginine
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
8.6
D-Arginine
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
11
D-Arginine
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
14
D-Arginine
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
20
D-Arginine
-
wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.7
D-Asn
-
Km less than 0.7 s-1, mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
13
D-Asn
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
50
D-Asn
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
62.4
D-Asn
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
106
D-Asn
-
pH 8.5, 25C, mutant enzyme M213R
1300
D-Asn
-
pH 8.5, 25C, wild-type enzyme
6.7
D-Asp
-
pH 8.5, 25C
29
D-Asp
-
pH 8.5, 25C, wild-type enzyme
235
D-Asp
-
pH 8.5, 25C, mutant enzyme M213R
1665
D-Asp
-
pH 8.5, 25C, mutant enzyme M213R/Y238R
0.09
D-Aspartate
-
mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.26
D-Aspartate
-
mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.37
D-Aspartate
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.5
D-Aspartate
-
mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value; wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.56
D-Aspartate
-
mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.67
D-Aspartate
-
mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
0.9
D-Aspartate
-
mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
1
D-Aspartate
-
mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
2.5
D-Aspartate
-
mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3.2
D-Aspartate
-
mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
3.9
D-Aspartate
-
mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6
D-Aspartate
-
mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25C, apparent value
6.7
D-Aspartate
-
at pH 7.0 and 25C
21.67
D-Dopa
-
in 50 mM sodium diphosphate buffer (pH 8.3) at 25C
21.7
D-Dopa
-
at pH 7.0 and 25C
290
D-Gln
-
pH 8.5, 25C, mutant enzyme M213R
1135
D-Gln
-
pH 8.5, 25C, wild-type enzyme
60
D-Glu
-
pH 8.5, 25C, wild-type enzyme
975
D-Glu
-
pH 8.5, 25C, mutant enzyme M213R
1.51
D-His
-
pH 8.3, 37C, recombinant DAO
76.7
D-Ile
-
pH 8.0, 37C
29.1
D-Leu
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
46
D-Leu
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
56
D-Leu
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
66.6
D-Leu
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
116
D-Leu
-
pH 8.0, 37C
0.8
D-Lys
-
wild-type enzyme
2.33
D-Lys
-
mutant enzymeY224G
3
D-Lys
-
mutant enzyme I223D/Y224G
3.33
D-Lys
-
mutant enzyme E220D/Y224G
3.54
D-Lys
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
5
D-Lys
-
mutant enzyme G222D/Y224G; mutant enzyme R221D/Y224G
42.3
D-Lys
-
pH 8.0, 37C
1.33
D-Met
-
mutant enzyme I223D/Y224G
1.38
D-Met
-
mutant enzymeY224G
2.5
D-Met
-
mutant enzyme G222D/Y224G
6.83
D-Met
-
wild-type enzyme
9
D-Met
-
mutant enzyme R221D/Y224G
10.5
D-Met
-
mutant enzyme E220D/Y224G
42
D-Met
-
mutant enzyme F258S, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
63
D-Met
-
mutant enzyme F258A, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
80.5
D-Met
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
89
D-Met
-
mutant enzyme F258Y, in 50 mM potassium phosphate buffer, pH 8.0, at 30C
680
D-Met
-
pH 8.5, 25C, mutant enzyme M213R
4600
D-Met
-
pH 8.5, 25C, wild-type enzyme
0.84
D-methionine
-
recombinant mutant C108S, pH 7.5, 37C, with 6-dichloroindophenolate
1.5
D-methionine
-
recombinant wild-type enzyme, pH 7.5, 37C, with 6-dichloroindophenolate
2.8
D-methionine
-
recombinant mutant C108D, pH 7.5, 37C, with 6-dichloroindophenolate
47.5
D-methionine
-
mutant Y228F
75
D-methionine
-
recombinant mutant C108D, pH 7.5, 37C, with O2
92
D-methionine
-
mutant Y224F
110
D-methionine
-
recombinant mutant C108S, pH 7.5, 37C, with O2
143
D-methionine
-
wild-type
200
D-methionine