Cloned (Comment) | Organism |
---|---|
expression of EGFP-tagged DAAO in U-87 glioblastoma cells, transient coexpression of pLG72 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoate | a classical substrate-competitive inhibitor, effect of benzoate on the FAD-binding process to hDAAO apoprotein | Homo sapiens | |
chlorpromazine | competitive versus cofactor FAD | Homo sapiens | |
additional information | benzoate and CPZ similarly modify the short-term cellular D-serine concentration but affect the cellular concentration of hDAAO differently | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | DAAO-lingand interaction kinetics, overview | Homo sapiens | |
4 | - |
D-serine | pH 8.5, 25°C, recombinant EGFP-DAAO | Homo sapiens | |
7 | - |
D-serine | pH 8.5, 25°C, recombinant wild-type DAAO | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine + H2O + O2 | Homo sapiens | the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
additional information | Homo sapiens | DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14920 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-serine + H2O + O2 | - |
Homo sapiens | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution | Homo sapiens | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
additional information | DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine | Homo sapiens | ? | - |
? | |
additional information | comparison of the kinetics of trypsin cleavage of hDAAO in the presence of various ligands, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAAO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
D-serine | pH 8.5, 25°C, recombinant EGFP-DAAO | Homo sapiens | |
3 | - |
D-serine | pH 8.5, 25°C, recombinant wild-type DAAO | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the apoprotein form of hDAAO binds the substrate D-serine, this interaction increases FAD binding thus increasing the amount of active holoenzyme in solution, binding analysis of FAD, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | in human brain the flavoprotein D-amino acid oxidase is responsible for the degradation of the neuromodulator D-serine, an important effector of NMDA-receptor mediated neurotransmission | Homo sapiens |