Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)pLysS and in Nicotiana tabacum plastids | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Schizosaccharomyces pombe |
39000 | - |
x * 39000, calculated from amino acid sequence | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-amino acid + H2O + O2 | Schizosaccharomyces pombe | - |
2-oxocarboxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Schizosaccharomyces pombe | pyruvate + NH3 + H2O2 | - |
? | |
D-amino acid + H2O + O2 | - |
Schizosaccharomyces pombe | 2-oxocarboxylate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Schizosaccharomyces pombe |
? | x * 39000, calculated from amino acid sequence | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Schizosaccharomyces pombe |
DAAO | - |
Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
physiological function | expression of a D-amino acid oxidase gene from Schizosaccharomyces pombe in tobacco chloroplasts give rise to transplastomic ptdao plants that resemble wild-type plants in growth and appearance. Growth of ptdao cells is tolerant to D-alanine but inhibited by D-valine | Schizosaccharomyces pombe |