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Literature summary for 1.4.3.3 extracted from

  • Boselli, A.; Sacchi, S.; Job, V.; Pilone, M.S.; Pollegioni, L.
    Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study (2002), Eur. J. Biochem., 269, 4762-4771.
    View publication on PubMed
Search for more literature for 1.4.3.3

Protein Variants

Protein Variants Comment Organism
Y238F 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 4.7fold lower than the wild-type value, Km-value for D-Pro is 1.7fold lower than the wild-type value, Km-value for D-Trp is 1.5fold lower than the wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3fold lower than the wild-type value, Km-value for D-Phe is 4.3fold lower than the wild-type value Rhodotorula toruloides
Y238S 3fold slower turnover on D-Ala as substrate than wild-type enzyme. KM-value for D-Ala is 2fold lower than the wild-type value, Km-value for D-Ser is 8fold lower than the wild-type value, Km-value for D-Pro is 1.6fold lower than the wild-type value, Km-value for D-Trp is identical to wild-type value, Km-value for cephalosporin is 2.6fold lower than the wild-type value, Km-value for D-Val is 3.2fold lower than the wild-type value, Km-value for D-Phe is 7.5fold lower than the wild-type value Rhodotorula toruloides

Inhibitors

Inhibitors Comment Organism Structure
benzoate competitive Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 D-Phe pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
0.07
-
 D-Phe pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
0.2
-
 D-Trp pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
0.26
-
 O2 pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
0.3
-
 D-Trp pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
0.3
-
 D-Phe pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
0.3
-
 D-Trp pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
0.96
-
 O2 pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
1.7
-
 D-Ser pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.9
-
 cephalosporin C pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
1.9
-
 cephalosporin C pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
2.3
-
 O2 pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
2.6
-
 D-Ala pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
2.9
-
 D-Ser pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
5
-
 cephalosporin C pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
6
-
 D-Val pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
6.1
-
 D-Val pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
7.5
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
7.8
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
12.3
-
 D-Pro pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
13.5
-
 D-Pro pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
13.7
-
 D-Ser pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
18.9
-
 D-Val pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
21.5
-
 D-Pro pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides P80324
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodotorula toruloides

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37
-
-
 Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-Ala + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
D-Phe + H2O + O2
-
 Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
 ?
D-Pro + H2O + O2
-
 Rhodotorula toruloides 2-oxopentanoate + NH3 + H2O2
-
 ?
D-Ser + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
D-Trp + H2O + O2
-
 Rhodotorula toruloides indol-3-pyruvate + NH3 + H2O2
-
 ?
D-Val + H2O + O2
-
 Rhodotorula toruloides alpha-ketoisovalerate + NH3 + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
 O2 pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
120
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238S Rhodotorula toruloides
125
-
 O2 pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
125
-
 D-Ala pH 8.5, 25°C, mutant enzyme Y238F Rhodotorula toruloides
350
-
 O2 pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
350
-
 D-Ala pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides