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Literature summary for 1.4.3.3 extracted from
- A conserved aspartate is essential for FAD binding and catalysis in the D-amino acid oxidase from Trigonopsis variabilis (1998), FEBS Lett., 436, 119-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Trigonopsis variabilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D206A | decreased activity with D-amino acids | Trigonopsis variabilis |
| D206E | decreased activity with D-amino acids | Trigonopsis variabilis |
| D206G | no activity with D-amino acids | Trigonopsis variabilis |
| D206L | no activity with D-amino acids | Trigonopsis variabilis |
| D206N | no activity with D-amino acids | Trigonopsis variabilis |
| D206S | decreased activity with D-amino acids | Trigonopsis variabilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.63 | - |
D-alanine | wild-type | Trigonopsis variabilis |  |
| 9.36 | - |
D-alanine | mutant D206E | Trigonopsis variabilis | |
| 18.12 | - |
D-alanine | mutant D206A | Trigonopsis variabilis | |
| 20.52 | - |
D-alanine | mutant D206S | Trigonopsis variabilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trigonopsis variabilis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.04 | - |
D206A | Trigonopsis variabilis |
| 6.41 | - |
D206S | Trigonopsis variabilis |
| 16.71 | - |
D206E | Trigonopsis variabilis |
| 18.61 | - |
wild-type | Trigonopsis variabilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Trigonopsis variabilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000167 | - |
D-alanine | mutant D206A | Trigonopsis variabilis | |
| 0.001 | - |
D-alanine | mutant D206S | Trigonopsis variabilis | |
| 0.00233 | - |
D-alanine | mutant D206E | Trigonopsis variabilis | |
| 0.00567 | - |
D-alanine | wild-type | Trigonopsis variabilis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 FAD per monomer of MW 38000-39000 | Trigonopsis variabilis | |
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Release 2023.1 (January 2023)
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