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Literature summary for 1.4.3.3 extracted from

  • Schraeder, T.; Andreesen, J.R.
    Properties and chemical modification of D-amino acid oxidase from Trigonopsis variabilis (1996), Arch. Microbiol., 165, 41-47.
No PubMed abstract available
Search for more literature for 1.4.3.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 D-phenylalanine
-
 Trigonopsis variabilis
0.29
-
 D-methionine
-
 Trigonopsis variabilis
0.38
-
 D-phenylglycine
-
 Trigonopsis variabilis
0.83
-
 cephalosporin C
-
 Trigonopsis variabilis
3
-
 D-valine
-
 Trigonopsis variabilis
6.5
-
 D-alanine
-
 Trigonopsis variabilis
17.3
-
 D-serine
-
 Trigonopsis variabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 PAGE Trigonopsis variabilis
320000
-
 PAGE Trigonopsis variabilis
570000
-
 gel filtration Trigonopsis variabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cephalosporin C + H2O + O2 Trigonopsis variabilis
-
 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-alanine + H2O + O2 Trigonopsis variabilis
-
 pyruvic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2 Trigonopsis variabilis
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2 Trigonopsis variabilis
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2 Trigonopsis variabilis
-
 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
D-valine + H2O + O2 Trigonopsis variabilis
-
 alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Trigonopsis variabilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
 Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-alanine + H2O + O2
-
 Trigonopsis variabilis pyruvic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2
-
 Trigonopsis variabilis 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2
-
 Trigonopsis variabilis phenylpyruvic acid + NH3 + H2O2
-
 ?
D-phenylglycine + H2O + O2
-
 Trigonopsis variabilis benzoylformic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2
-
 Trigonopsis variabilis 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2
-
 Trigonopsis variabilis 3-hydroxypyruvate + NH3 + H2O2
-
 r
D-valine + H2O + O2
-
 Trigonopsis variabilis alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Trigonopsis variabilis