Application | Comment | Organism |
---|---|---|
medicine | polyethylene glycol-conjugated D-amino acid oxidase exhibits potent antitumor activity by generating toxic reactive oxygen species, namely oxidation therapy, subsequently shows remarkable antitumor effect on murine sarcoma 180 solid tumor | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, native D-amino acid oxidase, SDS-PAGE | Sus scrofa |
65000 | - |
2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE | Sus scrofa |
113000 | - |
recombinant polyethylene glycol-conjugated D-amino acid oxidase, gel filtration | Sus scrofa |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-114 | less than 10% decrease of enzyme activity after Triton X-114 treatment, sonication, lyophilization or repeated freezing-thawing | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap DEAE column chromatography | Sus scrofa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
recombinant polyethylene glycol-conjugated D-amino acid oxidase, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) | Sus scrofa |
5.3 | - |
native enzyme, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Sus scrofa | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Sus scrofa | pyruvate + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | - |
Sus scrofa | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | - |
Sus scrofa | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
glycine + 2 H2O + O2 | - |
Sus scrofa | 2 formic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39000, native D-amino acid oxidase, SDS-PAGE | Sus scrofa |
homodimer | 2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Sus scrofa |
PEG-DAO | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sus scrofa |