Information on EC 1.1.5.2 - glucose 1-dehydrogenase (PQQ, quinone)

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
1.1.5.2
-
RECOMMENDED NAME
GeneOntology No.
glucose 1-dehydrogenase (PQQ, quinone)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glucose and glucose-1-phosphate degradation
-
-
glucose degradation (oxidative)
-
-
glycogen metabolism
-
-
L-ascorbate biosynthesis VI (engineered pathway)
-
-
Pentose phosphate pathway
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glucose:ubiquinone oxidoreductase
Integral membrane protein containing PQQ as prosthetic group. It also contains bound ubiquinone and Mg2+ or Ca2+. Electron acceptor is membrane ubiquinone but usually assayed with phenazine methosulfate. Like in all other quinoprotein alcohol dehydrogenases the catalytic domain has an 8-bladed propeller structure. It occurs in a wide range of bacteria. Catalyses a direct oxidation of the pyranose form of D-glucose to the lactone and thence to D-gluconate in the periplasm. Oxidizes other monosaccharides including the pyranose forms of pentoses.
CAS REGISTRY NUMBER
COMMENTARY hide
81669-60-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain L.M.D. 79.41, enzyme s-GDH
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
LMD 79.41
-
-
Manually annotated by BRENDA team
strain LMD79.41
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain YU423
-
-
Manually annotated by BRENDA team
gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ
UniProt
Manually annotated by BRENDA team
gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ
UniProt
Manually annotated by BRENDA team
strain P4 (soil-isolate)
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene gdhS
-
-
Manually annotated by BRENDA team
gene gdhS
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
alfalfa plants inoculated with RmH580, a Sinorhizobium meliloti gcd mutant strain show a delay in nodule emergence and a reduced ability for nodulation at various inoculum dosages. Mutant RmH580 strain is also deficient in its competitive ability. In coinoculation experiments a mutant/wild-type inoculum ratio higher than 100:1 is necessary to obtain an equal ratio of nodule occupancy. PQQ-linked GCD is required by Sinorhizobium meliloti for optimal nodulation efficiency and competitiveness on alfalfa roots
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-D-glucose + pyrroloquinoline quinone
2-amino-D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
-
-
-
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindolphenol
2-deoxy-D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
2-deoxy-D-glucose + oxidized N-methylphenazonium methyl sulfate
2-deoxy-D-glucono-1,5-lactone + ruduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
2-deoxy-D-glucose + pyrroloquinoline quinone
2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
2-deoxy-D-glucose + ubiquinone
2-deoxy-D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
3-deoxy-D-glucose + pyrroloquinoline quinone
3-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
-
-
-
-
?
3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol
3-O-methyl-D-glucono-1,5-lactone + ?
show the reaction diagram
3-O-methyl-D-glucose + pyrroloquinoline quinone
3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
3-O-methyl-D-glucose + ubiquinone
3-O-methyl-D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
6-deoxy-D-glucose + pyrroloquinoline quinone
6-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
-
-
-
-
?
allose + 2,6-dichlorophenolindolphenol
?
show the reaction diagram
allose + ubiquinone
? + ubiquinol
show the reaction diagram
beta-D-glucose + ubiquinone
beta-D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
cellobiose + 2,6-dichlorophenolindolphenol
?
show the reaction diagram
-
-
-
?
cellobiose + pyrroloquinoline quinone
?
show the reaction diagram
-
70% of the activity with D-glucose
-
-
?
cellobiose + ubiquinone
? + ubiquinol
show the reaction diagram
D-allose + 2,6-dichlorophenolindophenol
D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-allose + pyrroloquinoline quinone
D-allono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-allose + ubiquinone
? + ubiquinol
show the reaction diagram
-
-
-
-
?
D-cellobiose + 2,6-dichloroindophenol
D-cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-fructose + 2,6-dichloroindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-fucose + N-ethylphenazonium ethyl sulfate
6-deoxy-D-galactono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-fucose + oxidized N-methylphenazonium methyl sulfate
6-deoxy-D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
D-fucose + pyrrolquinoline quinone
6-deoxy-D-galactono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
-
-
-
-
?
D-fucose + ubiquinone
? + ubiquinol
show the reaction diagram
-
-
-
-
?
D-galactose + 2,6-dichloroindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-galactose + 2,6-dichlorophenolindolphenol
D-galactono-1,5-lactone + ?
show the reaction diagram
D-galactose + 2,6-dichlorophenolindophenol
D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-galactose + oxidized N-methylphenazonium methyl sulfate
D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
D-galactose + pyrroloquinoline quinone
D-galactono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-galactose + ubiquinone
?
show the reaction diagram
-
-
-
?
D-galactose + ubiquinone
D-galactono-1,5-lactone + ubiquinol
show the reaction diagram
D-glucose + 1,4-naphthoquinone
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 2,3-dichloro-1,4-naphthoquinone
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-glucose + 2,6-dichlorophenol-indophenol
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 2,6-dichlorophenolindolphenol
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-glucose + 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-glucose + 2-methyl-6-methoxy-1,4-benzoquinone
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 4-(4-ferrocenylimino-methyl)phenol
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + 4-ferrocenylnitrophenol
D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-glucose + 4-ferrocenylphenol
D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-glucose + 9,10-phenanthrenequinone
D-glucono-1,5-lactone + ?
show the reaction diagram
-
9,10-phenanthrenequinone shows low effectivity as redox mediator
-
-
?
D-glucose + ?
D-glucono-1,5-lactone + ?
show the reaction diagram
-
physiological electron acceptor in not known
-
-
?
D-glucose + ferricyanide
D-glucono-1,5-lactone + ferrocyanide
show the reaction diagram
D-glucose + N-ethylphenazonium ethyl sulfate
D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-glucose + oxidized N-methylphenazonium methyl sulfate
D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
D-glucose + phenazine methosulfate
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + pyrroloquinoline quinone
D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-glucose + trimethyl-1,4-benzoquinone
D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-glucose + ubiquinone
D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
D-glucose + ubiquinone Q1
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + ubiquinone Q2
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + ubiquinone Q4
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + ubiquinone Q6
D-glucono-1,5-lactone + ?
show the reaction diagram
D-glucose + ubiquinone Q9
D-glucono-1,5-lactone +
show the reaction diagram
D-lactose + 2,6-dichlorophenolindophenol
D-lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-maltose + 2,6-dichlorophenolindophenol
D-maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
71% activity compared to D-glucose
-
-
?
D-maltose + pyrroloquinoline quinone
?
show the reaction diagram
-
-
-
-
?
D-mannose + 2,6-dichloroindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-mannose + 2,6-dichlorophenolindolphenol
?
show the reaction diagram
-
-
-
?
D-mannose + 2,6-dichlorophenolindophenol
D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
36% activity compared to D-glucose
-
-
?
D-mannose + pyrroloquinoline quinone
D-mannono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-mannose + ubiquinone
? + ubiquinol
show the reaction diagram
D-melibiose + pyrroloquinoline quinone
?
show the reaction diagram
D-ribose + 2,6-dichloroindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-ribose + pyrroloquinoline quinone
D-ribono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
-
-
-
-
?
D-ribose + ubiquinone
? + ubiquinol
show the reaction diagram
-
-
-
?
D-xylose + 2,6-dichloroindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-xylose + 2,6-dichlorophenolindolphenol
D-xylono-1,5-lactone + ?
show the reaction diagram
-
-
-
?
D-xylose + 2,6-dichlorophenolindophenol
D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
86% activity compared to D-glucose
-
-
?
D-xylose + N-ethylphenazonium ethyl sulfate
D-xylono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
D-xylose + oxidized N-methylphenazonium methyl sulfate
D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
D-xylose + pyrroloquinoline quinone
D-xylono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-xylose + ubiquinone
D-xylono-1,5-lactone + ubiquinol
show the reaction diagram
L-arabinose + 2,6-dichloroindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
L-arabinose + 2,6-dichlorophenolindophenol
L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
97% activity compared to D-glucose
-
-
?
L-arabinose + N-ethylphenazonium ethyl sulfate
L-arabino-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
L-arabinose + oxidized N-methylphenazonium methyl sulfate
L-arabino-1,5-lactone + reduced N-methylphenazonium methyl sulfate
show the reaction diagram
-
-
-
-
?
L-arabinose + pyrroloquinoline quinone
L-arabino-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
L-arabinose + ubiquinone
L-arabino-1,5-lactone + ubiquinol
show the reaction diagram
L-lyxose + pyrroloquinoline quinone
?
show the reaction diagram
-
-
-
-
?
L-rhamnose + pyrroloquinoline quinone
L-rhamnono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
lactose + 2,6-dichlorophenol-indophenol
?
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenolindolphenol
?
show the reaction diagram
lactose + pyrroloquinoline quinone
?
show the reaction diagram
lactose + ubiquinone
?
show the reaction diagram
-
-
-
?
lactose + ubiquinone
? + ubiquinol
show the reaction diagram
maltose + 2,6-dichloroindophenol
maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
maltose + 2,6-dichlorophenolindolphenol
?
show the reaction diagram
maltose + pyrroloquinoline quinone
?
show the reaction diagram
maltose + ubiquinone
?
show the reaction diagram
-
-
-
?
maltose + ubiquinone
? + ubiquinol
show the reaction diagram
melibiose + 2,6-dichlorophenol-indophenol
?
show the reaction diagram
-
-
-
-
?
phenazine methosulfate + 2,6-dichlorophenol indophenol
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-D-glucose + ubiquinone
beta-D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
-
-
-
-
?
D-glucose + ?
D-glucono-1,5-lactone + ?
show the reaction diagram
-
physiological electron acceptor in not known
-
-
?
D-glucose + pyrroloquinoline quinone
D-glucono-1,5-lactone + pyrroloquinoline quinol
show the reaction diagram
D-glucose + ubiquinone
D-glucono-1,5-lactone + ubiquinol
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyrroloquinoline quinone
additional information
-
ruthenium(III) bispyridine compounds and ruthenium(III) 4-methyl-bispyridine compounds can act as artificial electron transfer mediator system
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
mutants D354N, N355D, and D354N/N355D
Cd2+
-
can replace Ca2+ in reactivation after thermal inactivation
Mn2+
-
can replace Ca2+ in reactivation after thermal inactivation
Sr2+
mutants D354N, N355D, and D354N/N355D
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aptameric enzyme subunit
-
the aptameric enzyme subunit inhibits PQQGDH in the presence of adenosine
-
Ba2+
competitive to Mg2+, wild-type enzyme
Ca2+
competitive to Mg2+, wild-type enzyme
D-glucose
EDTA
-
3.3 mM, complete inhibition
methylhydrazine
Mg2+
competitive to Ca2+, Sr2+, or Ba2+, mutants D354N, N355D, and D354N/N355D
p-benzoquinone
-
1.7 mM, complete inhibition
PGa4
-
aptamer PGa4 reduces enzymatic activity to 80% at 25 nM
-
pyrroloquinoline quinone
-
substrate inhibition at high concentrations
Sr2+
competitive to Mg2+, wild-type enzyme
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mag2
-
the PQQGDH aptameric enzyme subunit Mag2 increases activity of the enzyme by 20%
-
pyrroloquinoline quinone
exogenously added cofector slightly activates the enzyme activity
additional information
-
the aptameric enzyme subunit does not activate PQQGDH in the absence of adenosine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6
2,6-dichlorophenolindophenol
-
pH 8.8, 25C
9.5
2-amino-D-glucose
-
wild-type enzyme
1.6 - 90
2-deoxy-D-glucose
10.8
3-deoxy-D-glucose
-
wild-type enzyme
2 - 198
3-O-methyl-D-glucose
0.73 - 2.43
4-(4-ferrocenylimino-methyl)phenol
0.89 - 2.73
4-ferrocenylnitrophenol
0.83 - 1
4-ferrocenylphenol
1.3
6-deoxy-D-glucose
-
wild-type enzyme
21 - 199
allose
14 - 17
cellobiose
1.5 - 810
D-Allose
390
D-fructose
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
5 - 12
D-fucose
2 - 390
D-galactose
0.23 - 680
D-glucose
19 - 910
D-mannose
17.7
D-melibiose
-
wild-type enzyme
40 - 166
D-ribose
5.5 - 500
D-xylose
0.69
ferricyanide
-
pH 8.8, 25C
0.118 - 46
L-arabinose
100
L-Lyxose
-
wild-type enzyme
0.66 - 77
lactose
10 - 600
maltose
150
maltotriose
-
-
0.56
N,N,N,N-tetramethyl-o-phenylenediamine
-
pH 8.8, 25C
0.268 - 0.292
N-ethylphenazonium ethyl sulfate
0.064 - 0.362
N-methylphenazonium methyl sulfate
0.074 - 1.9
phenazine methosulfate
0.00011
pyrroliquinoline quinone
-
pH 7.0, 25C, wild-type enzyme
0.00005 - 0.78
pyrroloquinoline quinone
0.06 - 0.178
ubiquinone Q1
0.012 - 0.061
ubiquinone Q2
0.028 - 0.031
ubiquinone Q4
0.0034 - 0.019
ubiquinone Q6
0.0044
ubiquinone Q9
-
membrane-bound enzyme form
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
331 - 1060
2-deoxy-D-glucose
215 - 3200
3-O-methyl-D-glucose
73 - 2509
allose
226 - 1355
cellobiose
1440 - 4560
D-Allose
4.3 - 630
D-galactose
0.8 - 4424
D-glucose
3.1 - 861
D-mannose
3.2 - 678
D-xylose
167 - 1800
lactose
2.3 - 2870
maltose
2270
maltotriose
Escherichia coli
-
-
additional information
additional information
Acinetobacter calcoaceticus
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2900
D-cellobiose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
1716
400
D-fructose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
117
11000
D-galactose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
71
8.2 - 6100
D-glucose
3400
D-mannose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
216
1700
D-ribose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
292
6500
D-xylose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
115
4100
L-arabinose
Pyrobaculum aerophilum
-
in 50 mM Bis-Tris propane (pH 8.0), at 50C
206
3900
maltose
Pyrobaculum aerophilum
-
in 50 mM Bis-ris propane (pH 8.0), at 50C
86
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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inhibitory effects of the different metal ions on recombinant wild-type and mutant enzymes
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00245
native enzyme in cells of strain SC17(0), pH 6.5, temperature not specified in the publication
0.0197
native enzyme in cells of strain SC17(0) in presence of 0.01 mM pyrroloquinoline quinone, pH 6.5, temperature not specified in the publication
0.037
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cells grown on a phosphate-sufficient minimal medium M9
0.058
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cells grown on a phosphate-deficient medium TRP
0.77
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purified enzyme,in 50 mM BisTris propane (pH 8.0), at 50C
250
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purified native s-GDH
570
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membrane bound enzyme form
2209
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soluble enzyme form
2500
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mutant N340F/Y418I
2642
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purified recombinant mutant chimeric isozyme PQQGDH-B
2800
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mutant T416V/T417V
3030
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wild-type isozyme PQQGDH-B
3100
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mutant N340F/Y418F
4512
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purified recombinant Arg-tagged isozyme PQQGDH-B
4610
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purified recombinant wild-type isozyme PQQGDH-B
5080
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purified recombinant isozyme PQQGDH-B
5811
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purified recombinant His-tagged enzyme, pH 7.0, 25C
7400
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recombinant enzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
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reaction with potassium ferricyanide
5.5
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native m-GDH
6 - 9
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immobilized s-GDH, broad maximum
7.5
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the highest activity is observed with 42 mM sodium potassium phosphate buffer at pH 7.5
8
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the optimum pH for D-glucose oxidation at 50C is around pH 8.0
8.5
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membrane-bound enzyme
9
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reaction with pyrroloquinoline quinone
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
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pH 4.0: about 50% of maximal activity, pH 8.0: about 35% of maximal activity
7.5 - 9.5
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pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
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LITERATURE
25 - 40
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at 25C the enzyme activity is reduced to 50%, whereas at 40C the activity is only reduced to 90% compared to the activity at 37C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 4.7
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calculated, recombinant cytochrome c-fusion protein; calculated, wild-type GDH-B
SOURCE TISSUE
ORGANISM
UNIPROT
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LITERATURE
SOURCE
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