Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
recombinant high-level production of soluble His-tagged pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli strain BL21 (DE3), 6fold improvement improvement of sPQQGDH expression in MMBL medium | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | isozyme mPQQGDH | Acinetobacter calcoaceticus | 16020 | - |
additional information | there are two types of PQQGDH, the membrane-bound glucose dehydrogenase and the soluble glucose dehydrogenase | Acinetobacter calcoaceticus | - |
- |
periplasm | soluble isozyme sPQQGDH | Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | three ions per enzyme subunit | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Acinetobacter calcoaceticus | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | Acinetobacter calcoaceticus L.M.D. 79.41 | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter calcoaceticus L.M.D. 79.41 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme 11.3fold from Escherichia coli strain BL21 (DE3)by nickel affinity chromatography | Acinetobacter calcoaceticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | effects of different carbon sources and glucose concentrations on the cell growth and sPQQGDH production, overview | Acinetobacter calcoaceticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5811 | - |
purified recombinant His-tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
PQQGDH | - |
Acinetobacter calcoaceticus |
pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified recombinant enzyme, 10 min, stable | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | required for catalysis, one molecule per enzyme subunit | Acinetobacter calcoaceticus |
General Information | Comment | Organism |
---|---|---|
additional information | the purified recombinant PQQGDH shows thermal stability and substrate specificity as the native enzyme | Acinetobacter calcoaceticus |