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Literature summary for 1.1.5.2 extracted from

  • Sara-Paez, M.; Contreras-Zentella, M.; Gomez-Manzo, S.; Gonzalez-Valdez, A.A.; Gasca-Licea, R.; Mendoza-Hernandez, G.; Escamilla, J.E.; Reyes-Vivas, H.
    Purification and characterization of the membrane-bound quinoprotein glucose dehydrogenase of Gluconacetobacter diazotrophicus PAL 5 (2015), Protein J., 34, 48-59.
    View publication on PubMed
Search for more literature for 1.1.5.2

Inhibitors

Inhibitors Comment Organism Structure
Myxothiazol less than 10% residual activity at 0.1 mM Gluconacetobacter diazotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
0.5
-
 2-deoxy-D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
40
-
 D-galactose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 x * 86000, SDS-PAGE Gluconacetobacter diazotrophicus

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
 formerly Acetobacter diazotrophicus
-
Gluconacetobacter diazotrophicus PAL 5
-
 formerly Acetobacter diazotrophicus
-

Purification (Commentary)

Purification (Comment) Organism
QAE-Toyopearl column chromatography, CM-Toyopearl column chromatography, and Sephacryl S-200 gel filtration Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
112
-
 with D-galactose, at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
126
-
 with 2-deoxy-D-glucose, at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
130
-
 with D-glucose, at pH 7.0 and 25°C Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + ubiquinone 96% activity compared to D-glucose Gluconacetobacter diazotrophicus 2-deoxy-D-glucono-1,5-lactone + ubiquinol
-
 ?
2-deoxy-D-glucose + ubiquinone 96% activity compared to D-glucose Gluconacetobacter diazotrophicus PAL 5 2-deoxy-D-glucono-1,5-lactone + ubiquinol
-
 ?
3-methyl-alpha-methyl-D-glucopyranose + ubiquinone 20% activity compared to D-glucose Gluconacetobacter diazotrophicus D-xylono-1,5-lactone + ubiquinol
-
 ?
3-methyl-alpha-methyl-D-glucopyranose + ubiquinone 20% activity compared to D-glucose Gluconacetobacter diazotrophicus PAL 5 D-xylono-1,5-lactone + ubiquinol
-
 ?
D-galactose + ubiquinone 86% activity compared to D-glucose Gluconacetobacter diazotrophicus D-galactono-1,5-lactone + ubiquinol
-
 ?
D-galactose + ubiquinone 86% activity compared to D-glucose Gluconacetobacter diazotrophicus PAL 5 D-galactono-1,5-lactone + ubiquinol
-
 ?
D-glucose + decylubiquinone
-
 Gluconacetobacter diazotrophicus D-glucono-1,5-lactone + decylubiquinol
-
 ?
D-glucose + phenazine methosulfate
-
 Gluconacetobacter diazotrophicus ?
-
 ?
D-glucose + ubiquinone Q1
-
 Gluconacetobacter diazotrophicus D-glucono-1,5-lactone + ubiquinol Q1
-
 ?
D-glucose + ubiquinone Q2
-
 Gluconacetobacter diazotrophicus D-glucono-1,5-lactone + ubiquinol Q2
-
 ?
D-xylose + ubiquinone 40% activity compared to D-glucose Gluconacetobacter diazotrophicus D-xylono-1,5-lactone + ubiquinol
-
 ?
D-xylose + ubiquinone 40% activity compared to D-glucose Gluconacetobacter diazotrophicus PAL 5 D-xylono-1,5-lactone + ubiquinol
-
 ?
additional information less than 8% activity with D-mannitol, D-sorbitol, D-trehalose, D-arabinose, D-glucose 6-phosphate, methal-alpha-D-glucopyranose, D-mannose, sucrose, D-maltose, and D-lactose. No activity with gluconic acid and D-fructose Gluconacetobacter diazotrophicus ?
-
 ?
additional information less than 8% activity with D-mannitol, D-sorbitol, D-trehalose, D-arabinose, D-glucose 6-phosphate, methal-alpha-D-glucopyranose, D-mannose, sucrose, D-maltose, and D-lactose. No activity with gluconic acid and D-fructose Gluconacetobacter diazotrophicus PAL 5 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 86000, SDS-PAGE Gluconacetobacter diazotrophicus
? x * 86389, mass spectrometry Gluconacetobacter diazotrophicus

Synonyms

Synonyms Comment Organism
GDHm
-
 Gluconacetobacter diazotrophicus
membrane-bound glucose dehydrogenase
-
 Gluconacetobacter diazotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 no activity after 10 min at 40°C Gluconacetobacter diazotrophicus
46
-
 half-life temperature of the holoenzyme Gluconacetobacter diazotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160.5
-
 D-galactose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
180.6
-
 2-deoxy-D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
186.3
-
 D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Gluconacetobacter diazotrophicus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Gluconacetobacter diazotrophicus

pI Value

Organism Comment pI Value Maximum pI Value
Gluconacetobacter diazotrophicus isoelectric focusing
-
 6

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0085
-
 at pH 7.0 and 25°C Gluconacetobacter diazotrophicus Myxothiazol

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 D-galactose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
360
-
 2-deoxy-D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
1860
-
 D-glucose at pH 7.0 and 25°C Gluconacetobacter diazotrophicus