Application | Comment | Organism |
---|---|---|
biotechnology | a Glucose sensitive biosensor containing GDH immobilized on Prussian blue (PB)-modified graphite electrode is designed. Properties of the biosensor are investigated in the cathodic and anodic response detection regions. It is shown, that anodic response of the biosensor is sum of two signals: direct electron transport from reduced pyrroloquinoline quinine to the electrode and by formation of the pyrroloquinoline quinone-oxygen-Prussion blue-carbon ternary complex. Cathodic response of the biosensor is based on the oxidation of the reduced pyrroloquinoline quinone by Prussian blue-oxygen-Prussian blue complex. Electrochemical regeneration of the enzyme does not produce free hydrogen peroxide | Erwinia sp. |
Organism | UniProt | Comment | Textmining |
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Erwinia sp. | - |
- |
- |
Purification (Comment) | Organism |
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the enzyme is purified (not further specified). The enzyme stock solution has an activity of 64 U/ml and a concentration of 20 mg protein/ml. For the GDH immobilization, 2 ml of the enzyme stock solution in the 0.05 M phosphate buffer (pH 6) are applied onto the active area of the Prussian Blue-modified graphite electrode. After air-drying the electrode is incubated for 10 min in glutaraldehyde vapor 25% solution, and then kept in refrigerator | Erwinia sp. |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Erwinia sp. |
glucose dehydrogenase | - |
Erwinia sp. |
Cofactor | Comment | Organism | Structure |
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pyrroloquinoline quinone | - |
Erwinia sp. |