Any feedback?
Literature summary for 1.1.5.2 extracted from
- Surface charge engineering of PQQ glucose dehydrogenase for downstream processing (2003), Biotechnol. Lett., 25, 1695-1701.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | surface charge engineering of the enzyme for optimization of downstream processing in large scale enzyme production | Acinetobacter calcoaceticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in the periplasm of Escherichia coli strain PP2418, with and without a C-terminal tag of 3 Arg residues | Acinetobacter calcoaceticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q209R/N240R/T389R | site-directed mutagenesis, increased thermal stability compared to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Acinetobacter calcoaceticus | |
| 2 | - |
D-galactose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus |  |
| 4 | - |
D-galactose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 5 | - |
D-galactose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 10 | - |
maltose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 11 | - |
maltose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 13 | - |
maltose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 25 | - |
D-glucose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 25 | - |
lactose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 26 | - |
lactose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 27 | - |
D-glucose | recombinant wild-type enzyme and tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 36 | - |
lactose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 41 | - |
3-O-methyl-D-glucose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 53 | - |
3-O-methyl-D-glucose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 56 | - |
3-O-methyl-D-glucose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 63 | - |
allose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 75 | - |
allose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
| 76 | - |
allose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Acinetobacter calcoaceticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | P13650 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| optimization of the purification process involving cation exchange chromatography in presence of Zn2+ | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ? | - |
? | |
| allose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| D-galactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ? | - |
? | |
| D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| PQQGDH | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant tagged wild-type enzyme and mutant enzyme show about 90% remaining activity after 10 min, the nontagged wild-type enzyme shows about 40% remaining activity after 10 min | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | i.e. PQQ | Acinetobacter calcoaceticus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
