Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.5.2 extracted from

  • Oubrie, A.; Rozeboom, H.J.; Dijkstra, B.W.
    Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: A covalent cofactor-inhibitor complex (1999), Proc. Natl. Acad. Sci. USA, 96, 11787-11791.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.5.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-galactose Acinetobacter calcoaceticus
0.118
-
 L-arabinose pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
0.156
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-xylose Acinetobacter calcoaceticus
0.178
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
0.22
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-fucose Acinetobacter calcoaceticus
0.23
-
 D-glucose pH 8.5, reaction with 2,6-dichlorophenolindophenol as electron acceptor Acinetobacter calcoaceticus
0.268
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-xylose Acinetobacter calcoaceticus
0.278
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
0.291
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-fucose Acinetobacter calcoaceticus
0.292
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with L-arabinose Acinetobacter calcoaceticus
0.362
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with 2-deoxy-D-glucose Acinetobacter calcoaceticus
4
-
 D-glucose pH 8.5, reaction with N-methylphenazonium methyl sulfate or N-ethylphenazonium ethal sulfate as electron acceptor Acinetobacter calcoaceticus
5
-
 D-fucose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
5.9
-
 D-glucose pH 8.5, reaction with Wurster‘ Blue as electron acceptor Acinetobacter calcoaceticus
7
-
 D-xylose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
12
-
 D-xylose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
12
-
 D-fucose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
18
-
 L-arabinose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
19
-
 L-arabinose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
19
-
 D-galactose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
22
-
 2-deoxy-D-glucose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
612
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
D-fucose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus 6-deoxy-D-galactono-1,5-lactone + ?
-
 ?
D-fucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus 6-deoxy-D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
D-galactose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
D-xylose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + ?
-
 ?
D-xylose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
L-arabinose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + ?
-
 ?
L-arabinose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone reconstitution of the apoenzyme to full activity is achieved with a stoichiometric amount of pyrroloquinoline quinone. Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor Acinetobacter calcoaceticus