Information on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.1.99.18
-
RECOMMENDED NAME
GeneOntology No.
cellobiose dehydrogenase (acceptor)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
mechanism
-
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
mechanism of electron transfer
-
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
mechanism: cellobiose rapidly reduces the flavin group, which in turn slowly reduces the cytochrome b moiety, cytochrome b may donate electrons very rapidly to either mammalian cytochrome c or bacterial cytochrome c-551. Reactions are second-order
-
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
mechanism of electron transfer
Phanerochaete chrysosporium K 3
-
-
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
mechanism
Phanerochaete chrysosporium CMI 172727
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidation
-
-
oxidation
Chaetomium sp.
-
-
oxidation
Q6BDD5
-
oxidation
-
-
oxidation
Q8J2T4
-
oxidation
-
-
oxidation
Q6AW20
-
oxidation
Q74253
-
oxidation
Q7Z975
-
oxidation
Q42729
-
oxidation
Q9P8H5
-
oxidation
Monilia sp.
-
-
oxidation
Q4WZA6
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
cellobiose:acceptor 1-oxidoreductase
2,6-Dichloroindophenol can act as acceptor. Also acts, more slowly, on cello-oligosaccharides, lactose and D-glucosyl-1,4-beta-D-mannose. Includes EC 1.1.5.1, cellobiose dehydrogenase (quinone), which is now known to be a proteolytic product of this enzyme. The enzyme from the white rot fungus Phanerochaete chrysosporium is unusual in having two redoxin domains, one containing a flavin and the other a protoheme group. It transfers reducing equivalents from cellobiose to two types of redox acceptor: two-electron oxidants, including redox dyes, benzoquinones and molecular oxygen and one-electron oxidants, including semiquinone species, iron(II) complexes and the model acceptor cytochrome c [9].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cbdA
-
gene name
cbdA
Thielavia terrestris NRRL8126
-
gene name
-
CBO
-
-
-
-
Cdh
-
-
-
-
Cdh
Q7Z975
-
Cdh
Chaetomium sp.
-
-
Cdh
Q6BDD5
-
Cdh
Q8J2T4
-
Cdh
Q9P8H5
-
Cdh
Q6AW20
-
Cdh
Magnaporthe grisea, Monilia sp.
-
-
Cdh
Myriococcum thermophilum CBS 208.89
-
;
-
Cdh
Thielavia terrestris NRRL8126
-
-
-
Cdh
Trametes cinnabarina ss3
-
-
-
Cdh
Trametes cotonea
-
-
Cdh
Trametes cotonea CBS 352.80
-
-
-
Cdh
Trametes gibbosa MB 187
-
-
-
Cdh
Trametes hirsuta MB 50
-
-
-
Cdh
Trametes incerta
-
-
Cdh
Trametes incerta CBS 455.76
-
-
-
Cdh
Trametes maxima CBS 250.69
-
-
-
Cdh
Trametes ochracea MB 49
-
-
-
Cdh
Trametes suaveolens MB 51
-
-
-
Cdh
Trametes versicolor MB 52
-
-
-
CDH IIA
-
isoform
CDH IIB
-
isoform
CDH1
-
isoform
CDH1
Neurospora crassa FGSC 2489
-
; isoform
-
Cdh2
-
isoform
Cdh2
Neurospora crassa FGSC 2489
-
isoform
-
cellobiose (acceptor) 1-oxidoreductase
B6D1P2
-
cellobiose (acceptor) 1-oxidoreductase
Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
cellobiose dehydrogenase
-
-
-
-
cellobiose dehydrogenase
Q4WZA6
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Q7Z975
-
cellobiose dehydrogenase
Athelia rolfsii CBS 191.62
-
; CDH
-
cellobiose dehydrogenase
B6D1P2
-
cellobiose dehydrogenase
Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
cellobiose dehydrogenase
Chaetomium sp.
-
-
cellobiose dehydrogenase
Q6BDD5
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
Coriolopsis trogii 201
-
-
-
cellobiose dehydrogenase
Q8J2T4
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
Q9P8H5
-
cellobiose dehydrogenase
Q6AW20
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
Q74240
-
cellobiose dehydrogenase
-
; formerly called cellobiose oxidase
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Myriococcum thermophilum CBS 208.89
-
CDH; formerly called cellobiose oxidase
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
Neurospora crassa FGSC 2489
-
-
-
cellobiose dehydrogenase
Q01738
-
cellobiose dehydrogenase
Phanerochaete chrysosporium K3
-
-
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
Phanerochaete sordida MB 66
-
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Q74253
-
cellobiose dehydrogenase
Trametes cinnabarina MUCL39533
-
CDH
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Trametes pubescens MBB89
-
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Trametes sanguinea MUCL 41582 PS7
-
CDH
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Q42729
-
cellobiose dehydrogenase
Q875J3
-
cellobiose dehydrogenase
-
-
cellobiose dehydrogenase
-
CDH
cellobiose dehydrogenase
Trametes villosa CBS 334.49
-
-
-
cellobiose deydrogenase
-
-
cellobiose oxidase
Q4WZA6
-
cellobiose oxidase
-
-
cellobiose oxidase
Q7Z975
-
cellobiose oxidase
Chaetomium sp.
-
-
cellobiose oxidase
Q6BDD5
-
cellobiose oxidase
Q8J2T4
-
cellobiose oxidase
Q9P8H5
-
cellobiose oxidase
Q6AW20
-
cellobiose oxidase
-
-
cellobiose oxidase
Q74240
-
cellobiose oxidase
-
-
cellobiose oxidase
A9XK88
-
cellobiose oxidase
Myriococcum thermophilum CBS 208.89
-
-
-
cellobiose oxidase
-
-
cellobiose oxidase
Q01738
-
cellobiose oxidase
Q74253
-
cellobiose oxidase
-
-
cellobiose oxidase
Q42729
-
cellobiose oxidase
-
-
cellobiose oxidoreductase
-
-
Cellobiose-quinone oxidoreductase
-
-
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q4WZA6
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q7Z975
-
cellobiose:(acceptor) 1-oxidoreductase
Chaetomium sp.
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q6BDD5
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q8J2T4
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q9P8H5
-
cellobiose:(acceptor) 1-oxidoreductase
Q6AW20
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q74240
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Myriococcum thermophilum CBS 208.89
-
-
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q01738
-
cellobiose:(acceptor) 1-oxidoreductase
Q74253
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:(acceptor) 1-oxidoreductase
Q42729
-
cellobiose:(acceptor) 1-oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q4WZA6
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q7Z975
-
cellobiose:quinone oxidoreductase
Chaetomium sp.
-
-
cellobiose:quinone oxidoreductase
Q6BDD5
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q8J2T4
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q9P8H5
-
cellobiose:quinone oxidoreductase
Q6AW20
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q74240
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q01738
-
cellobiose:quinone oxidoreductase
Q74253
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:quinone oxidoreductase
Q42729
-
cellobiose:quinone oxidoreductase
-
-
cellobiose:[acceptor] 1-oxidoreductase
-
-
cellobiose:[acceptor] 1-oxidoreductase
Neurospora crassa FGSC 2489
-
-
-
DCHsr
Athelia rolfsii CBS 191.62
-
-
-
dehydrogenase, cellobiose
-
-
-
-
MtCDH
Myriococcum thermophilum CBS 208.89
-
-
-
oxidase, cellobiose
-
-
-
-
TvCDH
Trametes villosa CBS 334.49
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
54576-85-1
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Athelia, CBS 191.62, very efficient producer of the enzyme
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
strain CBS 191.62
-
-
Manually annotated by BRENDA team
Athelia rolfsii CBS 191.62
strain CBS 191.62
-
-
Manually annotated by BRENDA team
strain FP-90031
UniProt
Manually annotated by BRENDA team
Ceriporiopsis subvermispora FP-90031
strain FP-90031
UniProt
Manually annotated by BRENDA team
Chaetomium sp.
-
-
-
Manually annotated by BRENDA team
Chaetomium sp.
Ascomycete
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
produces cellobiose oxidase
-
-
Manually annotated by BRENDA team
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
Manually annotated by BRENDA team
Coniophora puteana (Schum ex Fr) Karsten
strain EMPA 62, brown-rot fungus
-
-
Manually annotated by BRENDA team
Coniophora puteana (Schum ex Fr) Karsten EMPA
strain EMPA 62, brown-rot fungus
-
-
Manually annotated by BRENDA team
strain 201, DSM 11919
-
-
Manually annotated by BRENDA team
Coriolopsis trogii 201
strain 201, DSM 11919
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
INBI-2-26(-), mycelial sterilia, nonsporulating, optimal growth temperature 28C, isolated from dioxin-containing tropical soils (South Vietnam)
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
mRNA; Basidiomycete
SwissProt
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
Ascomycete
SwissProt
Manually annotated by BRENDA team
soft-rot thermophilic fungus
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
Monilia sitophila
-
-
-
Manually annotated by BRENDA team
Monilia sitophila
inducible enzyme
-
-
Manually annotated by BRENDA team
Monilia sp.
Ascomycete
-
-
Manually annotated by BRENDA team
Sporotrichum thermophile, Ascomycete
SwissProt
Manually annotated by BRENDA team
; CBS 208.89
-
-
Manually annotated by BRENDA team
strain CBS 208.89
-
-
Manually annotated by BRENDA team
Myriococcum thermophilum CBS 208.89
-
-
-
Manually annotated by BRENDA team
Myriococcum thermophilum CBS 208.89
CBS 208.89
-
-
Manually annotated by BRENDA team
Myriococcum thermophilum CBS 208.89
strain CBS 208.89
-
-
Manually annotated by BRENDA team
Ascomycete
-
-
Manually annotated by BRENDA team
Neurospora crassa FGSC 2489
-
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
earlier name: Sporotrichum pulverulentum
-
-
Manually annotated by BRENDA team
earlier name: Sporotrichum pulverulentum; strain K 3; white-rot fungus
-
-
Manually annotated by BRENDA team
identical with Sporotrichum pulverulentum, 3 isoenzymes; white-rot fungus
-
-
Manually annotated by BRENDA team
name for perfect stage of this fungus, imperfect stage is named Sporotrichum pulverulentum; white-rot fungus
-
-
Manually annotated by BRENDA team
strain ATCC 32629; white-rot fungus
-
-
Manually annotated by BRENDA team
strain CMI 172727; white-rot fungus
-
-
Manually annotated by BRENDA team
strain K 3; white-rot fungus
-
-
Manually annotated by BRENDA team
strain OGC 101, white-rot fungus
-
-
Manually annotated by BRENDA team
variant M65H
SwissProt
Manually annotated by BRENDA team
Phanerochaete chrysosporium CMI 172727
strain CMI 172727
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium K 3
strain K 3
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium K3
strain K3
SwissProt
Manually annotated by BRENDA team
Phanerochaete chrysosporium K3
strain K3
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium ME-446
strain ME-446
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium OGC 101
strain OGC 101, white-rot fungus
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium RP78
-
SwissProt
Manually annotated by BRENDA team
strain MB 66
-
-
Manually annotated by BRENDA team
Phanerochaete sordida MB 66
strain MB 66
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
strain AS 5.391, white-rot fungus
-
-
Manually annotated by BRENDA team
Schizophyllum commune AS 5.391
strain AS 5.391, white-rot fungus
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
Thielavia terrestris NRRL8126
-
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
strain MUCL39533 PC330
-
-
Manually annotated by BRENDA team
strain ss3
-
-
Manually annotated by BRENDA team
Trametes cinnabarina MUCL39533
strain MUCL39533 PC330
-
-
Manually annotated by BRENDA team
Trametes cinnabarina ss3
strain ss3
-
-
Manually annotated by BRENDA team
Trametes cotonea
CBS 352.80
-
-
Manually annotated by BRENDA team
Trametes cotonea CBS 352.80
CBS 352.80
-
-
Manually annotated by BRENDA team
Trametes gibbosa MB 187
MB 187
-
-
Manually annotated by BRENDA team
IFO 4917
-
-
Manually annotated by BRENDA team
white-rot basidiomycota
-
-
Manually annotated by BRENDA team
Trametes hirsuta MB 50
MB 50
-
-
Manually annotated by BRENDA team
Trametes incerta
CBS 455.76
-
-
Manually annotated by BRENDA team
Trametes incerta CBS 455.76
CBS 455.76
-
-
Manually annotated by BRENDA team
CBS 250.69
-
-
Manually annotated by BRENDA team
Trametes maxima CBS 250.69
CBS 250.69
-
-
Manually annotated by BRENDA team
CBS 453.76, weak activity
-
-
Manually annotated by BRENDA team
Trametes ochracea MB 49
MB 49
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
strain MBB89, CBS 696.94
-
-
Manually annotated by BRENDA team
white-rot basidiomycota
-
-
Manually annotated by BRENDA team
Trametes pubescens MBB89
strain MBB89, CBS 696.94
-
-
Manually annotated by BRENDA team
strain MUCL 41582 PS7
-
-
Manually annotated by BRENDA team
Trametes sanguinea MUCL 41582 PS7
strain MUCL 41582 PS7
-
-
Manually annotated by BRENDA team
Trametes suaveolens MB 51
MB 51
-
-
Manually annotated by BRENDA team
Basidiomycete
SwissProt
Manually annotated by BRENDA team
white-rot basidiomycota
-
-
Manually annotated by BRENDA team
Trametes versicolor MB 52
MB 52
-
-
Manually annotated by BRENDA team
Basidiomycete
-
-
Manually annotated by BRENDA team
CBS 334.49
-
-
Manually annotated by BRENDA team
strain CBS 334.49
-
-
Manually annotated by BRENDA team
white-rot basidiomycota
-
-
Manually annotated by BRENDA team
Trametes villosa CBS 334.49
CBS 334.49
-
-
Manually annotated by BRENDA team
Trametes villosa CBS 334.49
strain CBS 334.49
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose + 2,6-dichlorophenol indophenol
2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
20% of the activity with lactose
-
-
?
4-beta-glucosylmannose + acceptor
?
show the reaction diagram
-
47% the rate of cellobiose oxidation
-
-
?
4-beta-glucosylmannose + acceptor
?
show the reaction diagram
Monilia sitophila
-
47% the rate of cellobiose oxidation
-
-
?
4-beta-glucosylmannose + O2
?
show the reaction diagram
-
slowly oxidized
-
-
?
4-beta-glucosylmannose + O2
?
show the reaction diagram
-
50% of the activity with lactose
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + acceptor
?
show the reaction diagram
Monilia sitophila
-
70% the rate of cellobiose oxidation
-
-
?
agarose + O2
?
show the reaction diagram
-
immediate reduction of enzyme
-
-
?
alpha-lactose + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
-
-
-
?
alpha-lactose + acceptor
4-O-(alpha-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
beta-D-lactose + benzoquinone
lactobiono-1,5-lactone + benzoquinol
show the reaction diagram
Myriococcum thermophilum, Myriococcum thermophilum CBS 208.89
-
-
-
-
?
beta-lactose + acceptor
4-O-(beta-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
beta-maltose + 2,6-dichlorophenol indophenol
4-O-beta-glucosyl-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
20% of the activity with lactose
-
-
?
carboxymethylcellulose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
cello-oligosaccharides + acceptor
?
show the reaction diagram
Monilia sitophila
-
67% the rate of cellobiose oxidation
-
-
?
cellobiose + 1,4-benzoquinone
cellobiono-1,5-lactone + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
cellobiose + 1,4-benzoquinone
cellobiono-1,5-lactone + reduced 1,4-benzoquinone
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
Q875J3
-
-
-
-
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C4
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C0
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C1
-
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
2,6-dichlorophenol-indolophenol decolouration assay
-
-
?
cellobiose + 2,6-dichloroindophenol
cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
cellobiose + 2,6-dichloroindophenol
?
show the reaction diagram
B6D1P2
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Q01738
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
best substrate
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes villosa CBS 334.49
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes cinnabarina ss3
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa FGSC 2489
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa FGSC 2489
-
best substrate
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Trametes sanguinea, Trametes sanguinea MUCL 41582 PS7
-
2,6-dichlorophenol-indophenol decolouration assay, decolourisation of the anthraquinonic dyes ABu62 and ABu281, no activity in RBu19
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris NRRL8126
-
-
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
?
show the reaction diagram
Coriolopsis trogii, Coriolopsis trogii 201
-
-
-
-
?
cellobiose + 2,6-dichlorophenolindophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Chaetomium sp.
-
DCPIP
-
-
?
cellobiose + 2,6-dichlorophenolindophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
DCPIP, activity assay
-
-
?
cellobiose + 3,5-di-tert-butylbenzoquinone
cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone
show the reaction diagram
Q875J3
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila, Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q6BDD5
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q8J2T4
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q6AW20
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q74253
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q42729
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sp.
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q74240
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q4WZA6
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
2,6-dichlorophenolindophenol as electron acceptor
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c, phenol blue, potassium ferricyanide at a low rate
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c, phenol blue, potassium ferricyanide at a low rate
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
electron acceptors: 2,6-dichlorophenolindophenol, benzoquinone, ferricyanide, cytochrome c, methylene blue, O2
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
may play an important role in the degradation of cellulose and lignin by wood-rotting fungi
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
the enzyme participates in lignocellulose degradation by white-rot fungi with a proposed role in the early events of wood degradation
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
2,6-dichlorophenolindophenol and cytochrome c(3+) (cyt c(3+)) as electron acceptors
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
acceptor: cytochrome c
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
reduction of molecular oxygen to hydrogen peroxide, and Fe3+ to Fe2+, cellulose depolymerization is studied using the model compound methyl beta-D-glucopyranoside
the formation of glucose, arabinose, gluconic acid, erythrulose and formaldehde is detected and a mechanism for the reaction is proposed
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
2,6-dichlorophenol-indophenol decolouration assay, decolourisation of the anthraquinonic dyes ABu62 and ABu281, no activity in RBu19
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium OGC 101
-
Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Schizophyllum commune AS 5.391
-
electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten EMPA
-
2,6-dichlorophenolindophenol as electron acceptor
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium ME-446
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Trametes cinnabarina MUCL39533
-
2,6-dichlorophenol-indophenol decolouration assay, decolourisation of the anthraquinonic dyes ABu62 and ABu281, no activity in RBu19
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobionolactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
Q01738
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
Q875J3
-
-
-
?
cellobiose + cytochrome c
cellobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
?
show the reaction diagram
-
-
-
-
?
cellobiose + cytochrome c
?
show the reaction diagram
B6D1P2
-
-
-
?
cellobiose + ferricyanide
?
show the reaction diagram
-
-
-
-
?
cellobiose + ferricytochrome c
cellobiono-1,5-lactone + ferrocytochrome c
show the reaction diagram
-
intact CBOR fully reduced with cellobiose, CBOR partially reduced by ascorbate and isolated ascorbate-reduced heme domain, all transfer electrons at similar rates to cytochrome c. Reduction of cationic one-electron acceptors via the heme group supports an electron transfer chain model
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
-
enzyme produces H2O2 in a slow process
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
-
superoxide anion as primary reduced oxygen product, H2O2 is possibly formed by dismutation of superoxide anion
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
162% of the activity with lactose
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
enzyme consumes 1 mol molecular oxygen per mol of substrate oxidized
reaction product is cellobionic acid, no H2O2 detected, but superoxide anion
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
enzyme consumes equimolar amounts of oxygen and cellobiose
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
molecular O2 as electron acceptor
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
molecular O2 as electron acceptor
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
molecular O2 as electron acceptor
product is H2O2 and the corresponding lactone
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
readily oxidized, cellobiose and cellohexaose with almost same activity
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
readily oxidized, cellobiose and cellohexaose with almost same activity
reaction product is cellobionic acid, no H2O2 detected, but superoxide anion
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose and probable in lignin degradation, physiologically significant electron acceptor may or may not be oxygen
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
CBO binds strongly to cellulose and the radical reducing activity may decrease repolimerization and precipitation of lignin-like polymers on the cellulose surface, thereby facilitating cellulose degradation
-
-
-
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
O2 as natural electron acceptor
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
O2 as natural electron acceptor
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation, enzyme is actively influencing lignin degradation and peroxidase activity
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
enzyme action might prevent transglycosylation reactions from taking place if high cellobiose concentrations build up
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
mechanism in which reactive oxygen species generation by the flavin of the enzyme subsequently reoxidizes the cellobiose oxidoreductase haem
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
molecular O2 as electron acceptor
product is H2O2 and the corresponding lactone
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
O2 as natural electron acceptor
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
molecular O2 as electron acceptor
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
role in cellulose and probable in lignin degradation, physiologically significant electron acceptor may or may not be oxygen
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
role in cellulose degradation, enzyme is actively influencing lignin degradation and peroxidase activity
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium CMI 172727
-
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
cellobiose + ubiquinone
cellobiono-1,5-lactone + reduced ubiquinone
show the reaction diagram
-
-
-
-
-
cellobiose + ubiquinone
cellobiono-1,5-lactone + reduced ubiquinone
show the reaction diagram
-
-
-
-
?
cellodextrin + O2
aldonic acid + H2O2
show the reaction diagram
-
high activity towards cellodextrins
-
?
cellodextrin + O2
aldonic acid + H2O2
show the reaction diagram
-
decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized
-
-
?
cellodextrin + O2
aldonic acid + H2O2
show the reaction diagram
-
decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized
-
?
cellodextrin + O2
aldonic acid + H2O2
show the reaction diagram
Phanerochaete chrysosporium, Phanerochaete chrysosporium K 3
-
higher cellodextrins
-
-
?
cellohexaose + 2,6-dichlorophenol indophenol
cellohexono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa, Neurospora crassa FGSC 2489
-
-
-
-
?
cellohexaose + acceptor
cellohexaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellohexaose + acceptor
cellohexaono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
cellopentaose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
cellopentaose + 2,6-dichlorophenol indophenol
cellopentaono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes villosa, Trametes pubescens, Trametes villosa CBS 334.49
-
-
-
-
?
cellopentaose + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
-
-
-
?
cellopentaose + 2,6-dichlorophenol indophenol
cellopentono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa, Neurospora crassa FGSC 2489
-
-
-
-
?
cellopentaose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
-
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium OGC 101
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
Schizophyllum commune AS 5.391
-
-
-
?
cellopentaose + acceptor
cellopentaono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten EMPA
-
-
-
?
cellopentaose + acceptor
?
show the reaction diagram
-
-
-
-
?
cellopentaose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
cellotetraose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol indophenol
cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol indophenol
cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol indophenol
cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes villosa, Trametes pubescens, Trametes villosa CBS 334.49
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol indophenol
cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa FGSC 2489
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
-
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium OGC 101
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
Schizophyllum commune AS 5.391
-
-
-
?
cellotetraose + acceptor
cellotetrono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten EMPA
-
-
-
?
cellotetraose + acceptor
cellotetraono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellotetraose + acceptor
?
show the reaction diagram
-
-
-
-
?
cellotetraose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
cellotriose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
cellotriose + 2,6-dichlorophenol indophenol
?
show the reaction diagram
-
-
-
-
?
cellotriose + 2,6-dichlorophenol indophenol
cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
cellotriose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
-
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium OGC 101
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
Schizophyllum commune AS 5.391
-
-
-
?
cellotriose + acceptor
cellotriono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten EMPA
-
-
-
?
cellotriose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
cellulose + FcPF6
cellobiono-1,5-lactone + reduced FcPF6
show the reaction diagram
-
-
-
-
?
cellulose + O2
?
show the reaction diagram
-
immediate reduction of enzyme
-
-
?
cellulose + O2
?
show the reaction diagram
Phanerochaete chrysosporium, Phanerochaete chrysosporium K 3
-
cellulose as electron donor, 10times lower oxidation rate than with cellobiose
-
-
?
cellulose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
chitin + O2
?
show the reaction diagram
-
immediate reduction of enzyme
-
-
?
cotton + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
crystalline cellulose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
D-galactose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
D-glucose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
Ceriporiopsis subvermispora, Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichloroindophenol
D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C0
-
-
-
?
D-glucose + 2,6-dichloroindophenol
D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C1
-
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
weak substrate
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
27% of the activity with lactose
-
-
?
D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes villosa CBS 334.49
-
-
-
-
?
D-glucose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
the enzyme shows enhanced activity with glucose at neutral pH values
-
-
?
D-glucose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + cytochrome c
?
show the reaction diagram
B6D1P2
-
-
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
E7D6C4
-
-
-
?
D-mannose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
D-xylose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
D-xylose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
galactobiose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
good substrate
-
-
?
galactose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
weak substrate
-
-
?
galactose + O2
?
show the reaction diagram
-
slowly oxidized
-
-
?
galactose + O2
?
show the reaction diagram
-
26% of the activity with lactose
-
-
?
galactose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
glucose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
weak substrate
-
-
?
glucose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
lactose + 1,4-benzoquinone
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
show the reaction diagram
-
-
-
-
?
lactose + 1,4-benzoquinone
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + 1,4-benzoquinol
show the reaction diagram
-
-
-
-
?
lactose + 1,4-benzoquinone
?
show the reaction diagram
Ceriporiopsis subvermispora, Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
-
?
lactose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichloroindophenol
?
show the reaction diagram
Ceriporiopsis subvermispora, Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
-
?
lactose + 2,6-dichloroindophenol
?
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C4
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C0
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
show the reaction diagram
E7D6C1
-
-
-
?
lactose + 2,6-dichloroindophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6dichloro-indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
good substrate
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes suaveolens MB 51, Trametes ochracea MB 49, Trametes versicolor MB 52, Trametes hirsuta MB 50, Trametes maxima CBS 250.69, Trametes gibbosa MB 187, Trametes cotonea CBS 352.80
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Neurospora crassa FGSC 2489
-
good substrate
-
-
?
lactose + 2,6-dichlorophenol indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
Trametes incerta CBS 455.76
-
-
-
-
?
lactose + 2,6-dichlorophenol indophenol
lactobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol-indophenol
?
show the reaction diagram
Trametes pubescens, Trametes pubescens MBB89
-
-
-
-
?
lactose + 2,6-dichlorophenol-indophenol
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol-indophenol
lactobiono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenolindophenol
lactobiono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
-
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
lactose + 3,5-di-tert-butyl-1,2-benzoquinone
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
show the reaction diagram
Phanerochaete chrysosporium K 3
-
-
-
-
-
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
-
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
60% the rate of cellobiose oxidation
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
60% the rate of cellobiose oxidation
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium OGC 101
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Schizophyllum commune AS 5.391
-
-
-
?
lactose + acceptor
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Coniophora puteana (Schum ex Fr) Karsten EMPA
-
-
-
?
lactose + acceptor
?
show the reaction diagram
-
-
-
-
?
lactose + acceptor
?
show the reaction diagram
-
low activity with maltose and glucose
-
-
?
lactose + acceptor
?
show the reaction diagram
-
no activity at maltose and glucose
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Trametes pubescens, Chaetomium sp.
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
-
acceptor: O2, 2,6-dichloroindophenol or 1,4-benzoquinone
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
Trametes villosa CBS 334.49, Phanerochaete sordida MB 66, Myriococcum thermophilum CBS 208.89, Athelia rolfsii CBS 191.62
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
Coriolopsis trogii 201
-
-
-
-
?
lactose + acceptor
? + reduced acceptor
show the reaction diagram
Phanerochaete chrysosporium K3
-
-
-
-
?
lactose + acceptor
lactobiono-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
Q875J3
-
-
-
-
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
-
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
Q875J3
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
E7D6C4
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
E7D6C0
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
E7D6C1
-
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
lactose binds considerably more weakly than cellobiose
-
-
?
lactose + cytochrome c
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
show the reaction diagram
Trametes suaveolens MB 51, Trametes ochracea MB 49, Trametes versicolor MB 52, Trametes hirsuta MB 50, Trametes maxima CBS 250.69, Trametes gibbosa MB 187, Trametes cotonea CBS 352.80, Trametes incerta CBS 455.76
-
-
-
-
?
lactose + cytochrome c
?
show the reaction diagram
-
-
-
-
?
lactose + cytochrome c
?
show the reaction diagram
Ceriporiopsis subvermispora, Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
-
?
lactose + cytochrome c
?
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
lactose + cytochrome c
lactobiono-1,5-lactone + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
lactose + Fe(CN)63-
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + Fe(CN)64-
show the reaction diagram
-
-
-
-
?
lactose + ferricenium
?
show the reaction diagram
B6D1P2
-
-
-
?
lactose + ferricenium ion
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ?
show the reaction diagram
-
-
-
-
?
lactose + ferricyanide
?
show the reaction diagram
B6D1P2
-
-
-
?
lactose + H2O + acceptor
lactobionic acid + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + O2
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
-
?
lactose + O2
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
-
?
lactose + O2
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
-
readily oxidized
product is lactobionic acid
?
lactose + O2
4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
-
readily oxidized
product is lactobionic acid
?
lactose + O2
? + H2O2
show the reaction diagram
Myriococcum thermophilum, Myriococcum thermophilum CBS 208.89
-
-
-
-
?
maltose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
maltose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
maltose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
maltose + 2,6-dichloroindophenol
?
show the reaction diagram
Ceriporiopsis subvermispora, Ceriporiopsis subvermispora FP-90031
B6D1P2
-
-
-
?
maltose + 2,6-dichloroindophenol
?
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
maltose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
maltose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
maltose + acceptor
4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone
show the reaction diagram
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
maltose + cytochrome c
?
show the reaction diagram
B6D1P2
-
-
-
?
maltose + O2
? + H2O2
show the reaction diagram
Myriococcum thermophilum, Myriococcum thermophilum CBS 208.89
-
-
-
-
?
maltose + O2
4-O-beta-glucosyl-glucono-1,5-lactone + H2O2
show the reaction diagram
-
-
-
?
maltose + O2
4-O-beta-glucosyl-glucono-1,5-lactone + H2O2
show the reaction diagram
-
22% of the activity with lactose
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
E7D6C4
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
E7D6C0
-
-
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
E7D6C1
-
-
-
?
maltotetraose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
maltotetraose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
maltotriose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
maltotriose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
mannobiose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
good substrate
-
-
?
mannopentaose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
mannose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
weak substrate
-
-
?
mannose + O2
?
show the reaction diagram
-
slowly oxidized
-
-
?
sophorose + O2
?
show the reaction diagram
-
17% of the activity with lactose
-
-
?
thiocellobiose + 2,6-dichlorophenol indophenol
thiocellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
thiocellobiose + acceptor
thiocellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
xylan + O2
?
show the reaction diagram
-
immediate reduction of enzyme
-
-
?
xylobiose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
xylobiono-1,4-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
good substrate
-
-
?
xylobiose + acceptor
xylobiono-1,4-lactone + reduced acceptor
show the reaction diagram
-
-
-
?
xylobiose + acceptor
xylobiono-1,4-lactone + reduced acceptor
show the reaction diagram
-
38% the rate of cellobiose oxidation
-
?
xylose + 2,6-dichlorophenol indophenol
? + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
-
weak substrate
-
-
?
xylotriose + 1,4-benzoquinone
? + reduced 1,4-benzoquinone
show the reaction diagram
-
-
-
-
?
mannose + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
not as substrates: glucose, xylose, cellulose, galactobiose, gentiobiose, H2O2 not detected as product
-
-
-
additional information
?
-
-
electron acceptors: cytochrome c, ferricyanide, 3,5-di-t-butyl-o-benzoquinone and triiodide ion, 1,2,4,5-tetramethoxybenzene cation radical
-
-
-
additional information
?
-
-
enzyme reduces cytochrome c, 200 times faster than with oxygen, 2,6-dichlorophenolindophenol, phenoxy- and cation-radicals, a vatiety of quinones and Fe(III) compounds, the latter reduced 35-50 times faster than with oxygen, enzyme has a cellulose-binding domain
-
-
-
additional information
?
-
-
electron acceptors: 3,5-di-t-butyl-o-benzoquinone, 2,6-dichlorophenolindophenol, cytochrome c
-
-
-
additional information
?
-
-
electron acceptors: great number of quinones and radicals, cytochrome c, Mn(III), ferricyanide, oxygen, reduction of Fe(III) and cytochrome c more rapid than of oxygen, enzyme reduces also compounds of ligninase and manganese peroxidase in absence of their substrates
-
-
-
additional information
?
-
-
not as substrates: maltose, sophorose, glucose, xylose, cellulose
-
-
-
additional information
?
-
-
substrates: disaccharides, some insoluble polysaccharides, but no monosaccharides, oxygen as electron acceptor, artificial electron acceptors: 2,6-dichlorophenolindophenol, potassium ferricyanide, benzyl viologen, not with: FMN, FAD, riboflavin
-
-
-
additional information
?
-
-
important enzyme in lignin and cellulose biodegradation
-
-
-
additional information
?
-
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
-
the enzyme does not produce H2O2 using D-fructose as substrate
-
-
-
additional information
?
-
-
when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol
-
-
-
additional information
?
-
-
isoform CDH IIA exhibits 4 to 5times higher apparent catalytic constants (kcat and Km values) than isoform CDH IIB for most tested carbohydrates
-
-
-
additional information
?
-
-
isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value
-
-
-
additional information
?
-
Trametes villosa CBS 334.49, Trametes suaveolens MB 51
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Phanerochaete chrysosporium K 3
-
electron acceptors: cytochrome c, ferricyanide, 3,5-di-t-butyl-o-benzoquinone and triiodide ion, 1,2,4,5-tetramethoxybenzene cation radical
-
-
-
additional information
?
-
Phanerochaete chrysosporium K 3
-
enzyme reduces cytochrome c, 200 times faster than with oxygen, 2,6-dichlorophenolindophenol, phenoxy- and cation-radicals, a vatiety of quinones and Fe(III) compounds, the latter reduced 35-50 times faster than with oxygen, enzyme has a cellulose-binding domain
-
-
-
additional information
?
-
Phanerochaete chrysosporium K 3
-
electron acceptors: 3,5-di-t-butyl-o-benzoquinone, 2,6-dichlorophenolindophenol, cytochrome c
-
-
-
additional information
?
-
Trametes ochracea MB 49
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Myriococcum thermophilum CBS 208.89
-
the enzyme does not produce H2O2 using D-fructose as substrate
-
-
-
additional information
?
-
Trametes versicolor MB 52
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Trametes cinnabarina ss3
-
important enzyme in lignin and cellulose biodegradation
-
-
-
additional information
?
-
Trametes hirsuta MB 50, Trametes maxima CBS 250.69
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Thielavia terrestris NRRL8126
-
when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol
-
-
-
additional information
?
-
Trametes gibbosa MB 187, Trametes cotonea CBS 352.80
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Neurospora crassa FGSC 2489
-
isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value
-
-
-
additional information
?
-
Trametes incerta CBS 455.76
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose + 1,4-benzoquinone
cellobiono-1,5-lactone + reduced 1,4-benzoquinone
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + 2,6-dichlorophenol-indophenol
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q6BDD5
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q8J2T4
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q6AW20
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q74253
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q42729
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sp.
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q74240
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q4WZA6
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Monilia sitophila
-
may play an important role in the degradation of cellulose and lignin by wood-rotting fungi
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
the enzyme participates in lignocellulose degradation by white-rot fungi with a proposed role in the early events of wood degradation
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
2,6-dichlorophenolindophenol and cytochrome c(3+) (cyt c(3+)) as electron acceptors
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
acceptor: cytochrome c
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
reduction of molecular oxygen to hydrogen peroxide, and Fe3+ to Fe2+, cellulose depolymerization is studied using the model compound methyl beta-D-glucopyranoside
the formation of glucose, arabinose, gluconic acid, erythrulose and formaldehde is detected and a mechanism for the reaction is proposed
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
Myriococcum thermophilum CBS 208.89
-
-
-
-
?
cellobiose + acceptor
cellobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
A9XK88
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose and probable in lignin degradation, physiologically significant electron acceptor may or may not be oxygen
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
CBO binds strongly to cellulose and the radical reducing activity may decrease repolimerization and precipitation of lignin-like polymers on the cellulose surface, thereby facilitating cellulose degradation
-
-
-
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
O2 as natural electron acceptor
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
O2 as natural electron acceptor
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
role in cellulose degradation, enzyme is actively influencing lignin degradation and peroxidase activity
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
-
enzyme action might prevent transglycosylation reactions from taking place if high cellobiose concentrations build up
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
O2 as natural electron acceptor
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
role in cellulose and probable in lignin degradation, physiologically significant electron acceptor may or may not be oxygen
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
show the reaction diagram
Phanerochaete chrysosporium K 3
-
role in cellulose degradation, enzyme is actively influencing lignin degradation and peroxidase activity
-
-
?
cellopentaose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellotetraose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
cellotriose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
Thielavia terrestris, Thielavia terrestris NRRL8126
-
-
-
-
?
D-glucose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
the enzyme shows enhanced activity with glucose at neutral pH values
-
-
?
D-xylose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
lactose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Trametes pubescens, Chaetomium sp.
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Chaetomium sp.
-
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
lactose + acceptor
4-0-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
maltose + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
-
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q01738
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q7Z975
-
-
-
?
maltose + acceptor
4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
show the reaction diagram
Q9P8H5
-
-
-
?
additional information
?
-
-
important enzyme in lignin and cellulose biodegradation
-
-
-
additional information
?
-
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
-
when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol
-
-
-
additional information
?
-
Trametes villosa CBS 334.49, Trametes suaveolens MB 51, Trametes ochracea MB 49, Trametes versicolor MB 52
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Trametes cinnabarina ss3
-
important enzyme in lignin and cellulose biodegradation
-
-
-
additional information
?
-
Trametes hirsuta MB 50, Trametes maxima CBS 250.69
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
additional information
?
-
Thielavia terrestris NRRL8126
-
when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol
-
-
-
additional information
?
-
Trametes gibbosa MB 187, Trametes cotonea CBS 352.80, Trametes incerta CBS 455.76
-
proposed role of the enzyme in the early events of lignocellulose degradation and wood colonisation
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome b
-
as prosthetic group
-
cytochrome b
-
1-2 mol cytochrome b reduced per mol cellobiose; as prosthetic group
-
cytochrome b
-
-
-
cytochrome c
-
the enzyme is an extracellular flavocytochrome
FAD
-
hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1
FAD
Coniophora puteana (Schum ex Fr) Karsten
-
-
FAD
-
1 heme per enzyme molecule, 1 FAD nucleotide per molecule
FAD
-
as prosthetic group
FAD
-
both the reductive and the oxidative half-reactions take place on the FAD domain
FAD
-
FAD domain has all properties of cellobiose:quinone oxidoreductase, and could be formed by proteolytic cleavage of CBO into 55 kDa FAD and 35 kDd heme domain by papain, cellulose-binding site is located on the FAD domain
FAD
-
the enzyme comprises two redox domains, one containing flavin adenine dinucleotide and the other protoheme
FAD
Q01738
crystal structure includes an alpha/beta-type FAD-binding subdomain, containing a seven-stranded beta sheet and six helices
FAD
Q7Z975
one dinucleotide per CDH molecule, also 6-hydroxy-FAD
FAD
Chaetomium sp.
-
one dinucleotide per CDH molecule
FAD
Q6BDD5
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
Q8J2T4
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
Q9P8H5
one dinucleotide per CDH molecule, also 6-hydroxy-FAD
FAD
Q6AW20
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
Q74240
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
Q01738
one dinucleotide per CDH molecule, also 6-hydroxy-FAD
FAD
Q74253
one dinucleotide per CDH molecule
FAD
-
one dinucleotide per CDH molecule
FAD
Q42729
one dinucleotide per CDH molecule, also 6-hydroxy-FAD
FAD
-
one dinucleotide per CDH molecule
FAD
Chaetomium sp.
-
-
flavin
-
flavohemoprotein, one flavin per enzyme molecule
flavin
-
flavoprotein, FAD as flavin component
flavin
-
1 mol cellobiose reduces 1 mol enzyme-bound flavin in the fast kinetic process
flavin
-
flavin component probably is the sugar dehydrogenating unit in the enzyme; flavohemoprotein, one flavin per enzyme molecule
flavin
-
flavohemoprotein
flavin
-
the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu
flavin
-
falvocytochrome. Binding of cellobiose to the active site inhibits electron transfer from flavin to haem
flavin
-
the enzyme is an extracellular flavocytochrome
flavin
-
recombinant enzyme contains 0.96 mol of flavin per mol of enzyme, wild-type enzyme contains 0.97 mol of flavin per mol of enzyme
flavin
-
flavoheme enzyme
flavin
Q01738
flavocytochrome
heme
-
the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain
heme
-
flavocytochrome. Binding of cellobiose to the active site inhibits electron transfer from flavin to heme
heme
-
the heme domain is a one-electron reducing system
heme
-
contains one heme cofactor per protein molecule
heme
-
flavoheme enzyme
heme
Q01738
flavocytochrome
heme
Q7Z975
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Chaetomium sp.
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q6BDD5
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q8J2T4
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q9P8H5
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q6AW20
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q74240
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q01738
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q74253
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Q42729
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
-
protoporphyrin IX, protoheme IX, heme b, one heme per CDH molecule
heme
Chaetomium sp.
-
flavohemoprotein
heme
-
cytochrome b-type heme
heme
-
cytochrome-b-type heme
heme
-
cytochrome-type heme b
heme b
-
hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1
heme b
Coniophora puteana (Schum ex Fr) Karsten
-
-
heme b
-
1 heme per enzyme molecule, 1 FAD nucleotide per molecule
heme b
-
flavohemoprotein; heme component serves for the storage of electrons to be incorporated into molecular oxygen for direct reduction to water
heme b
-
hemoprotein
heme b
-
heme domain increases the rate of electron transfer to one-electron acceptors
heme b
-
heme group: cytochrome b type, inactivated at higher pH levels, caused by conformational changes of protein, heme is necessary for reduction of cytochrome c, it acts as a single electron reductant, 35 kDa heme-containing domain
heme b
-
flavohemoprotein
heme b
-
cleavage of heme containing fragment by papain or V8 proteinase can convert CBO to cellobiose:quinone dehydrogenase, cytochrome b-type heme group used for storage and delivery of electrons from a two-electron donor, e.g. cellobiose, via FADH2 to one-electron acceptors, e.g. radicals, cytochrome c, ferricyanide and oxygen
protoheme
-
the enzyme comprises two redox domains, one containing flavin adenine dinucleotide and the other protoheme
additional information
-
limited proteolytic cleavage of the enzyme leads to an inactive heme peptide and an active FAD domain which has been termed cellobiose: quinone 1-oxidoreductase EC 1.5.1.1
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
the enzyme shows a 4fold increase in catalytic current at up to 100 mM Ca2+. There is a positive effect of metal cations, particularly Ca2+, on the electron transfer between the dehydrogenase domain and the cytochrome domain
Ca2+
-
the enzyme shows an increase in catalytic current for a factor of 2.4 at up to 100 mM Ca2+. There is a positive effect of metal cations, particularly Ca2+, on the electron transfer between the dehydrogenase domain and the cytochrome domain
K+
-
the addition of KCl (up to 100 mM) increases the catalytic currents up to 2fold
Mg2+
-
stimulates activity up to 2fold
NaF
-
50 mM, activation of reaction with lactose (0.0125 mM) to 120% of control
NaN3
-
1 mM, activation of reaction with lactose (0.0125 mM) to 110% of control
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,2-bipyridine
-
-
2,2-bipyridine
-
1 mM, 41% inhibition
Ag+
-
2 mM, 70% inhibition after 6 h
cellobiono-1,5-lactone
-
-
cellobionolactone
-
more than 0.59 mM inhibit CBO activity
cellobiose
Coniophora puteana (Schum ex Fr) Karsten
-
strong substrate inhibition
cellobiose
-
strong substrate inhibition at concentrations higher than 0.015 mM
cellobiose
-
two binding sites for the substrate: the active site and the inhibition site
cellobiose
-
substrate inhibition
cellobiose
B6D1P2
substrate inhibition
cytochrome c
-
substrate inhibition
cytochrome c
B6D1P2
substrate inhibition
Gentiobiose
-
1 mM, 22% inhibition of reaction with lactose, 0.00625 mM
Hg2+
-
2 mM, 20% inhibition after 6 h
lactobiono-1,5-lactone
-
weak product inhibition
-
methyl-beta-D-cellobioside
-
1 mM, 40% inhibition of reaction with lactose, 0.00625 mM
NaN3
-
0.5 mM, 15% inhibition of reaction with lactose, 0.0125 mM
SDS
-
5 mM, complete inhibition
Sodium azide
-
1 mM, 19% inhibition
Sodium cyanide
-
-
Sodium cyanide
-
1 mM, 70% inhibition
tri-N-acetylchitotriose
-
0.5 mM, 20% inhibition of reaction with lactose, 0.00625 mM
Zn2+
-
2 mM, 60% inhibition after 6 h
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.011
1,4-benzoquinone
-
pH 4.5, 30C, reaction with lactose
0.019
1,4-benzoquinone
-
pH 4.5, 30C, reaction with lactose
0.022
1,4-benzoquinone
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.026
1,4-benzoquinone
B6D1P2
cellobiose as substrate, pH 3.5
0.056
1,4-benzoquinone
A9XK88
mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.012
2,6-dichloroindophenol
B6D1P2
cellobiose as substrate, pH 4.5
140.4
2,6-dichloroindophenol
-
lactose as electron donor, at 40C
0.0024
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
0.0049
2,6-dichlorophenol indophenol
-
pH 4.5, 30C, reaction with lactose
0.0056
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
0.0058
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
0.0064
2,6-dichlorophenol indophenol
Q01738
pH 4.5, reaction with cellobiose, wild-type enzyme
0.0074
2,6-dichlorophenol indophenol
Q01738
pH 4.5, reaction with cellobiose, mutant enzyme M65H
0.0087
2,6-dichlorophenol indophenol
-
at pH 5.5 and 30C
0.0092
2,6-dichlorophenol indophenol
-
at pH 6.0 and 30C
0.0099
2,6-dichlorophenol indophenol
-
pH 4.5, 30C, reaction with lactose
0.01147
2,6-dichlorophenol indophenol
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.012
2,6-dichlorophenol indophenol
-
pH 4.0, 20C
0.016
2,6-dichlorophenol indophenol
-
pH 6.0, 20C
0.02253
2,6-dichlorophenol indophenol
-
-
0.033
2,6-dichlorophenol indophenol
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.0698
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
0.089
2,6-dichlorophenol indophenol
-
pH 8.0, 20C
0.0036
2,6-dichlorophenol-indophenol
-
-
0.078
2,6-dichlorophenol-indophenol
-
in 50 mM MES buffer (pH 6.0), at 25C
0.0024
2,6-dichlorophenolindophenol
-
at 25C
0.0036
2,6-dichlorophenolindophenol
-
-
0.0037
2,6-dichlorophenolindophenol
-
at 45C
0.012
2,6-dichlorophenolindophenol
-
in presence of 0.24 mM O2
0.017
2,6-dichlorophenolindophenol
-
in absence of O2
0.08
2,6-dichlorophenolindophenol
Monilia sitophila
-
-
0.22
2,6-dichlorophenolindophenol
-
-
0.01
2,6-dichlorphenolindophenol
-
-
0.026
2,6-dichlorphenolindophenol
-
-
0.5
3,5 di-t-butyl-o-benzoquinone
-
-
0.012
3,5-di-tert-butyl-1,2-benzoquinone
-
-
0.018
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30C, reaction with lactose
0.041
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30C, reaction with lactose
0.3846
alpha-lactose
-
-
54
alpha-lactose
-
-
0.132
benzoquinone
-
-
3
benzyl viologen
-
-
2
beta-lactose
-
70-75%
0.0003
cellobiose
-
electron acceptor cytochrome c from horse heart
0.0004
cellobiose
-
electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical
0.002
cellobiose
-
electron acceptor K3Fe(CN)6
0.002
cellobiose
-
at pH 5.0 and 30C
0.003
cellobiose
-
at pH 7.5 and 30C
0.0034
cellobiose
-
at 25C
0.0039
cellobiose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.0044
cellobiose
-
electron acceptors methylen green and blue
0.0045
cellobiose
Chaetomium sp.
-
-
0.00467
cellobiose
-
pH 6.0, 20C
0.0047
cellobiose
Chaetomium sp.
-
-
0.00476
cellobiose
-
pH 4.0, 20C
0.00496
cellobiose
-
pH 8.0, 20C
0.005
cellobiose
-
at pH 5.0 and 30C
0.00579
cellobiose
-
isoform CDH2, at pH 6.0 and 50C
0.0058
cellobiose
-
at 25C
0.0069
cellobiose
-
-
0.007
cellobiose
-
at pH 6.0 and 30C
0.007
cellobiose
-
at pH 5.0 and 30C
0.011
cellobiose
-
-
0.011
cellobiose
Q9P8H5
review, different references
0.012
cellobiose
Monilia sitophila
-
-
0.012
cellobiose
-
at pH 5.0 and 30C
0.015
cellobiose
-
electron acceptor 2,6-dichloroindophenol
0.016
cellobiose
-
pH 4.5, 23C, recombinant wild-type enzyme
0.016
cellobiose
Q01738
review, different references
0.017
cellobiose
-
pH 4.5, 23C, mutant enzyme N732A
0.018
cellobiose
Q01738
pH 4.5, electron acceptor: cytochrome c, wild-type enzyme
0.019
cellobiose
-
pH 4.5, 23C, mutant enzyme H689N
0.021
cellobiose
-
pH 4.5, 23C, mutant enzyme H689Q or mutant enzyme H689A
0.022
cellobiose
-
pH 4.5, 23C, mutant enzyme H689E
0.022
cellobiose
-
isoform CDH IIB, at pH 6.0 and 30C
0.025
cellobiose
-
-
0.025
cellobiose
-
electron acceptor 1,4-benzoquinone
0.025
cellobiose
Q01738
review, different references
0.02572
cellobiose
-
isoform CDH1, at pH 6.0 and 50C
0.03
cellobiose
-
-
0.031
cellobiose
-
electron acceptor meldola blue
0.031
cellobiose
-
pH 4.5, 23C, mutant enzyme H689V
0.032
cellobiose
-
at pH 6.0
0.034
cellobiose
Q9P8H5
review, different references
0.034
cellobiose
Q6AW20
-
0.035
cellobiose
Q01738
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H
0.035
cellobiose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
0.04
cellobiose
-
pH 4.5, 23C, mutant enzyme N732H
0.04
cellobiose
Q01738
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme
0.04
cellobiose
Q01738
review, different references
0.041
cellobiose
-
pH 4.5, 23C, mutant enzyme N732Q
0.0416
cellobiose
-
-
0.042
cellobiose
-
-
0.042
cellobiose
-
electron donor
0.042
cellobiose
Q01738
review, different references
0.042
cellobiose
-
at pH 4.5
0.0432
cellobiose
-
pH 4.0, 30C, wild-type enzyme
0.046
cellobiose
Q6BDD5
-
0.0518
cellobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.053
cellobiose
-
electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone
0.0581
cellobiose
-
pH 4.0, 30C, recombinant enzyme
0.06
cellobiose
-
purified wild type enzyme, pH and temperature not specified in the publication
0.072
cellobiose
E7D6C0
at pH 6.0 and 30C
0.075
cellobiose
-
2,6-dichloroindophenol as electron acceptor, at 40C
0.081
cellobiose
-
at pH 4.5
0.082
cellobiose
B6D1P2
cytochrome c as electron acceptor
0.108
cellobiose
Q875J3
pH 4.5, 25C, recombinant enzyme
0.11
cellobiose
Q01738
review, different references
0.111
cellobiose
-
pH 5.0, 30C
0.12
cellobiose
-
-
0.12
cellobiose
Q42729
-
0.12
cellobiose
-
at pH 4.5
0.125
cellobiose
E7D6C1
at pH 5.0 and 30C
0.128
cellobiose
Q875J3
pH 4.5, 25C, wild-type enzyme
0.14
cellobiose
B6D1P2
2,6-dichloroindophenol as electron acceptor
0.14
cellobiose
-
at pH 5.0 and 30C
0.16
cellobiose
-
at pH 5.0 and 30C
0.16
cellobiose
-
at pH 5.5 and 30C
0.17
cellobiose
-
pH 4.5, 23C, mutant enzyme N732D
0.17
cellobiose
E7D6C4
at pH 5.5 and 30C
0.181
cellobiose
-
-
0.21
cellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.21
cellobiose
-
at pH 4.5
0.23
cellobiose
-
pH 4.5, 23C, mutant enzyme N732E
0.55
cellobiose
-
electron acceptor 2,6-dimethyl-1,4-benzoquinone
0.9
cellobiose
-
isoform CDH IIA, at pH 6.0 and 30C
0.1
cellohexaose
-
-
0.1224
cellohexaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.0075
Cellopentaose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.017
Cellopentaose
-
-
0.043
Cellopentaose
-
isoform CDH IIB, at pH 6.0 and 30C
0.055
Cellopentaose
-
-
0.083
Cellopentaose
-
-
0.085
Cellopentaose
-
-
0.1131
Cellopentaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.196
Cellopentaose
-
isoform CDH IIA, at pH 6.0 and 30C
0.2222
Cellopentaose
-
-
0.46
Cellopentaose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.46
Cellopentaose
-
at pH 4.5
0.51
Cellopentaose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.54
Cellopentaose
-
-
1.4
Cellopentaose
-
at pH 4.5
0.0056
Cellotetraose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.0064
Cellotetraose
-
at 25C
0.011
Cellotetraose
-
at 25C
0.021
Cellotetraose
-
-
0.06
Cellotetraose
-
-
0.061
Cellotetraose
-
-
0.061
Cellotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
0.0666
Cellotetraose
-
-
0.085
Cellotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
0.0902
Cellotetraose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.204
Cellotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
0.2105
Cellotetraose
-
-
0.22
Cellotetraose
-
at pH 4.5
0.42
Cellotetraose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.42
Cellotetraose
-
at pH 4.5
0.47
Cellotetraose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.47
Cellotetraose
-
at pH 4.5
0.6
Cellotetraose
-
-
1.396
Cellotetraose
-
electron donor
0.0082
cellotriose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.019
cellotriose
-
-
0.045
cellotriose
-
isoform CDH IIB, at pH 6.0 and 30C
0.058
cellotriose
-
-
0.062
cellotriose
-
-
0.0625
cellotriose
-
-
0.0814
cellotriose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.1905
cellotriose
-
-
0.216
cellotriose
-
electron donor
0.23
cellotriose
-
isoform CDH IIA, at pH 6.0 and 30C
0.49
cellotriose
-
-
0.0003
cytochrome c
-
-
0.00067
cytochrome c
-
at 25C
0.00067
cytochrome c
-
mutant F166Y, cytochrome c reduction
0.00069
cytochrome c
-
pH 4.5, 30C, reaction with lactose
0.0008
cytochrome c
Q01738
pH 4.5, reaction with cellobiose, wild-type enzyme
0.00094
cytochrome c
B6D1P2
cellobiose as substrate, pH 3.5
0.001
cytochrome c
-
at 45C
0.0012
cytochrome c
-
-
0.00126
cytochrome c
-
pH 4.0, 30C, wild-type enzyme
0.0013
cytochrome c
-
electron acceptor
0.00144
cytochrome c
-
pH 4.0, 30C, recombinant enzyme
0.00146
cytochrome c
-
wild type, cytochrome c reduction
0.0018
cytochrome c
-
pH 4.5, 30C, reaction with lactose
0.0028
cytochrome c
-
-
0.0046
cytochrome c
-
at pH 7.5 and 30C
0.0049
cytochrome c
-
at pH 5.0 and 30C
0.0057
cytochrome c
-
at pH 5.0 and 30C
0.008
cytochrome c
-
at pH 5.0 and 30C
0.012
cytochrome c
Q875J3
pH 4.5, 25C, wild-type enzyme
0.013
cytochrome c
-
-
0.0158
cytochrome c
-
pH 6.0, 20C
0.017
cytochrome c
-
pH 4.0, 20C
0.0179
cytochrome c
-
mutant F166Y, cellobiose oxidation with electron acceptors
0.018
cytochrome c
Q875J3
pH 4.5, 25C, recombinant enzyme
0.01861
cytochrome c
-
-
0.0259
cytochrome c
-
at pH 5.0 and 30C
0.0286
cytochrome c
-
wild type, cellobiose oxidation with electron acceptors
0.0305
cytochrome c
-
pH 8.0, 20C
0.0537
cytochrome c
-
at pH 6.0 and 30C
0.093
cytochrome c
-
-
0.384
cytochrome c
-
-
21.5
cytochrome c
-
lactose as electron donor, at 40C
3600
D-galactose
-
isoform CDH IIB, at pH 6.0 and 30C
21
D-glucose
-
at pH 7.5 and 30C
54
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 25C
71
D-glucose
-
at pH 5.0 and 30C
79
D-glucose
E7D6C1
at pH 5.0 and 30C
87
D-glucose
E7D6C0
at pH 6.0 and 30C
120
D-glucose
-
at pH 5.0 and 30C
170
D-glucose
-
at pH 6.0 and 30C
230
D-glucose
-
at pH 5.0 and 30C
240
D-glucose
-
-
260
D-glucose
-
at pH 5.0 and 30C
380
D-glucose
Q01738
review, different references
387
D-glucose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
550
D-glucose
-
isoform CDH IIB, at pH 6.0 and 30C
770
D-glucose
-
at pH 5.0 and 30C
890
D-glucose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
890
D-glucose
-
-
890
D-glucose
-
at pH 4.5
1250
D-glucose
Q7Z975
-
1300
D-glucose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
1300
D-glucose
-
-
1300
D-glucose
-
at pH 4.5
1600
D-glucose
Q01738
review, different references
1700
D-glucose
-
at pH 5.0 and 30C
3300
D-glucose
B6D1P2
2,6-dichloroindophenol as electron acceptor
3700
D-glucose
-
isoform CDH IIA, at pH 6.0 and 30C
3800
D-glucose
B6D1P2
cytochrome c as electron acceptor
4000
D-glucose
-
at pH 5.5 and 30C
17000
D-glucose
E7D6C4
at pH 5.5 and 30C
10000
D-mannose
-
isoform CDH IIA, at pH 6.0 and 30C
13300
D-mannose
-
isoform CDH IIB, at pH 6.0 and 30C
1000
D-xylose
-
in 50 mM MES buffer (pH 6.0), at 25C
1545
D-xylose
-
isoform CDH IIB, at pH 6.0 and 30C
2770
D-xylose
-
isoform CDH IIA, at pH 6.0 and 30C
0.0122
DCPIP
-
electron acceptor
0.012
di-t-butylbenzoqinone
-
-
0.00032
ferricenium
B6D1P2
cellobiose as substrate, pH 3.5
0.00099
ferricenium ion
-
pH 4.5, 30C, reaction with lactose
0.0012
ferricenium ion
-
pH 4.5, 30C, reaction with lactose
0.0045
ferricyanide
-
at 25C
0.0052
ferricyanide
-
-
0.0067
ferricyanide
-
at 45C
0.011
ferricyanide
B6D1P2
cellobiose as substrate, pH 3.5
0.012
ferricyanide
-
-
0.071
ferricyanide
-
-
0.1379
ferricyanide
-
-
7
ferricyanide
-
-
0.74
Galactobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
634
galactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
157.1
glucose
-
2,6-diclorindophenol as electron acceptor, at 40C
726
glucose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.0055
K3Fe(CN)6
-
pH 4.5, 30C, reaction with lactose
0.0086
K3Fe(CN)6
-
pH 4.5, 30C, reaction with lactose
0.214
K3Fe(CN)6
-
electron acceptor
0.014
lactose
-
at pH 7.5 and 30C
0.0172
lactose
-
CDH modified plain screen printed electrodes at 0 mV, pH and temperature not specified in the publication
0.018
lactose
-
at pH 5.0 and 30C
0.023
lactose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.028
lactose
-
at pH 6.0 and 30C
0.0281
lactose
-
at 25C
0.032
lactose
-
at pH 5.0 and 30C
0.048
lactose
-
at pH 5.0 and 30C
0.05
lactose
-
at 45C
0.05
lactose
-
at pH 5.0 and 30C
0.051
lactose
-
-
0.051
lactose
Q9P8H5
review, different references
0.055
lactose
-
-
0.056
lactose
-
pH 6.0, 20C
0.056
lactose
Chaetomium sp.
-
-
0.06
lactose
-
-
0.089
lactose
-
isoform CDH IIB, at pH 6.0 and 30C
0.09
lactose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
0.14
lactose
-
2,6-diclorindophenol as electron acceptor, at 40C
0.185
lactose
E7D6C4
at pH 5.5 and 30C
0.2
lactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.2
lactose
-
at pH 5.0 and 30C
0.215
lactose
E7D6C1
at pH 5.0 and 30C
0.217
lactose
E7D6C0
at pH 6.0 and 30C
0.23
lactose
-
at pH 5.0 and 30C
0.27
lactose
-
pH 4.5, 23C, recombinant wild-type enzyme
0.27
lactose
Q01738
review, different references
0.278
lactose
-
at pH 6.0
0.28
lactose
-
pH 4.5, 23C, mutant enzyme H689A
0.29
lactose
-
pH 4.5, 23C, mutant enzyme H689Q
0.29
lactose
-
isoform CDH IIA, at pH 6.0 and 30C
0.325
lactose
-
at pH 5.5 and 30C
0.553
lactose
-
at pH 4.5
0.63
lactose
Q01738
review, different references
0.68
lactose
Q01738
review, different references
0.938
lactose
Q875J3
pH 4.5, 25C, recombinant enzyme
0.95
lactose
Q875J3
pH 4.5, 25C, wild-type enzyme
1.1
lactose
Q01738
review, different references
1.16
lactose
-
purified wild type enzyme, pH and temperature not specified in the publication
1.2
lactose
-
pH 4.5, 23C, mutant enzyme N732A
1.85
lactose
Q6AW20
-
2.126
lactose
-
electron donor
2.13
lactose
-
at pH 4.5
2.3
lactose
-
pH 4.5, 23C, mutant enzyme N732H
2.3
lactose
-
-
2.4
lactose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
2.4
lactose
-
-
2.4
lactose
-
at pH 4.5
3.7
lactose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
3.7
lactose
-
at pH 4.5
3.7
lactose
-
-
4
lactose
-
pH 4.5, 23C, mutant enzyme N732Q
4.3
lactose
B6D1P2
cytochrome c as electron acceptor
4.4
lactose
B6D1P2
2,6-dichloroindophenol as electron acceptor
11.4
lactose
-
pH 4.5, 23C, mutant enzyme N732D
16.6
lactose
-
pH 4.5, 23C, mutant enzyme N732E
2.2
maltose
-
at pH 5.0 and 30C
2.4
maltose
-
at pH 7.5 and 30C
2.5
maltose
-
in 50 mM MES buffer (pH 6.0), at 25C
3.44
maltose
-
isoform CDH IIB, at pH 6.0 and 30C
3.65
maltose
Q01738
review, different references
6.75
maltose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
7.1
maltose
-
at pH 5.0 and 30C
7.8
maltose
-
at pH 5.0 and 30C
8
maltose
E7D6C1
at pH 5.0 and 30C
8.86
maltose
-
2,6-diclorindophenol as electron acceptor, at 40C
11
maltose
-
-
11
maltose
Q9P8H5
review, different references
13
maltose
-
at pH 5.0 and 30C
15.5
maltose
E7D6C0
at pH 6.0 and 30C
17.2
maltose
-
isoform CDH IIA, at pH 6.0 and 30C
18
maltose
-
at pH 5.0 and 30C
24
maltose
-
at pH 5.0 and 30C
31
maltose
-
at pH 6.0 and 30C
50
maltose
E7D6C4
at pH 5.5 and 30C
120
maltose
-
at pH 5.5 and 30C
240
maltose
Q7Z975
-
240
maltose
Q01738
review, different references
280
maltose
B6D1P2
cytochrome c as electron acceptor
300
maltose
B6D1P2
2,6-dichloroindophenol as electron acceptor
350
maltose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
350
maltose
-
-
350
maltose
-
at pH 4.5
390
maltose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
390
maltose
-
-
390
maltose
-
at pH 4.5
5051
maltose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.71
maltotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
4.5
maltotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
35.7
maltotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
1.2
maltotriose
-
isoform CDH IIB, at pH 6.0 and 30C
34.3
maltotriose
-
isoform CDH IIA, at pH 6.0 and 30C
1.53
mannobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
8.4
Mannopentaose
-
isoform CDH IIB, at pH 6.0 and 30C
39.2
Mannopentaose
-
isoform CDH IIA, at pH 6.0 and 30C
5051
Mannose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.15
Mn(III)-malonate
-
-
0.15
Mn3+-malonate
-
-
0.037
O2
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.073
O2
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.0389
TBBQ
-
electron acceptor
1.8
thiocellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
2.3
thiocellobiose
-
-
2.5
thiocellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
0.0002
triiodide ion
-
-
-
0.056
ubiquinone
-
mutant F166Y, cellobiose oxidation with electron acceptors
0.0579
ubiquinone
-
wild type, cellobiose oxidation with electron acceptors
0.293
ubiquinone
-
mutant F166Y, ubiquinone reduction
0.326
ubiquinone
-
wild type, ubiquinone reduction
0.327
ubiquinone
-
pH 4.0, 30C, recombinant enzyme
0.333
ubiquinone
-
pH 4.0, 30C, wild-type enzyme
1.31
xylobiose
-
isoform CDH IIB, at pH 6.0 and 30C
2.81
xylobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
3.6
xylobiose
-
isoform CDH IIA, at pH 6.0 and 30C
5.4
xylobiose
-
-
7.1
xylobiose
-
-
7.3
xylobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
7.7
xylobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
5051
xylose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.81
xylotriose
-
isoform CDH IIB, at pH 6.0 and 30C
3
xylotriose
-
isoform CDH IIA, at pH 6.0 and 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
13.4
1,4-benzoquinone
A9XK88
mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
19.6
1,4-benzoquinone
-
-
20.8
1,4-benzoquinone
-
pH 4.5, 30C, reaction with lactose
27.5
1,4-benzoquinone
-
pH 4.5, 30C, reaction with lactose
43
1,4-benzoquinone
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
45
1,4-benzoquinone
B6D1P2
cellobiose as substrate, pH 3.5
16.8
2,6-dichloroindophenol
-
-
29
2,6-dichloroindophenol
B6D1P2
cellobiose as substrate, pH 4.5
4
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
4.1
2,6-dichlorophenol indophenol
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
6.4
2,6-dichlorophenol indophenol
-
pH 4.0, 20C
7.6
2,6-dichlorophenol indophenol
-
pH 8.0, 20C
11.3
2,6-dichlorophenol indophenol
-
pH 6.0, 20C
12.4
2,6-dichlorophenol indophenol
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
13.5
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
16.01
2,6-dichlorophenol indophenol
-
-
17
2,6-dichlorophenol indophenol
-
at pH 6.0 and 30C
22.6
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
24.8
2,6-dichlorophenol indophenol
-
at pH 5.5 and 30C
25
2,6-dichlorophenol indophenol
Q01738
pH 4.5, reaction with cellobiose, mutant enzyme M65H
25.6
2,6-dichlorophenol indophenol
-
pH 4.5, 30C, reaction with lactose
27
2,6-dichlorophenol indophenol
Q01738
pH 4.5, reaction with cellobiose, wild-type enzyme
38.1
2,6-dichlorophenol indophenol
-
pH 4.5, 30C, reaction with lactose
53.8
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
2.05
2,6-dichlorophenol-indophenol
-
in 50 mM MES buffer (pH 6.0), at 25C
33
2,6-dichlorophenol-indophenol
-
-
7.5
2,6-dichlorophenolindophenol
-
at 25C
18.3
2,6-dichlorophenolindophenol
-
at 45C
17
2,6-dichlorphenolindophenol
-
-
63
2,6-dichlorphenolindophenol
-
-
17
3,5 di-tert-butyl-o-benzoquinone
-
-
16.9
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30C, reaction with lactose
24.2
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30C, reaction with lactose
27
3,5-di-tert-butyl-1,2-benzoquinone
-
-
14.22
alpha-lactose
-
-
17
alpha-lactose
-
-
21
benzoquinone
-
-
0.002
cellobiose
-
pH 4.5, 23C, mutant enzyme H689V
0.003
cellobiose
-
pH 4.5, 23C, mutant enzyme H689A
0.004
cellobiose
-
pH 4.5, 23C, mutant enzyme H689N or mutant H689Q; pH 4.5, 23C, mutant enzyme N732D
0.009
cellobiose
-
pH 4.5, 23C, mutant enzyme H689E
0.1
cellobiose
Q01738
pH 4.5, electron acceptor: cytochrome c, mutant enzyme M65H
0.21
cellobiose
-
pH 4.5, 23C, mutant enzyme N732E
0.4
cellobiose
-
pH 4.5, 23C, mutant enzyme N732A
1
cellobiose
-
pH 4.5, 23C, mutant enzyme N732Q
1.23
cellobiose
-
in 50 mM MES buffer (pH 6.0), at 25C
2.1
cellobiose
-
at pH 5.0 and 30C
2.7
cellobiose
-
pH 4.5, 23C, mutant enzyme N732H
3.3
cellobiose
-
at pH 6.0 and 30C
3.6
cellobiose
-
ferricyanide as electron acceptor
4
cellobiose
-
pH 8.0, 20C
4.11
cellobiose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
5
cellobiose
-
electron acceptor 2,6-dimethoxy-1,4-benzoquinone
6.1
cellobiose
-
at 25C
7.7
cellobiose
-
at pH 5.0 and 30C
7.9
cellobiose
-
pH 4.0, 20C
8.9
cellobiose
-
pH 6.0, 20C
9.4
cellobiose
-
at pH 5.0 and 30C
10.5
cellobiose
Q01738
pH 4.5, electron acceptor: cytochrome c, wild-type enzyme
11.4
cellobiose
-
isoform CDH IIB, at pH 6.0 and 30C
11.8
cellobiose
-
at pH 7.5 and 30C
12.7
cellobiose
-
isoform CDH2, at pH 6.0 and 50C
13
cellobiose
-
-
13
cellobiose
-
at pH 5.0 and 30C
13.5
cellobiose
-
isoform CDH1, at pH 6.0 and 50C
14
cellobiose
-
-
15
cellobiose
-
electron acceptor 2,6-dimethyl-1,4-benzoquinone
15.5
cellobiose
-
pH 4.5, 23C, recombinant wild-type enzyme
16.1
cellobiose
-
at 25C
16.2
cellobiose
-
at pH 5.0 and 30C
17.65
cellobiose
-
-
18
cellobiose
-
electron acceptors methylene blue or methylene green
19.9
cellobiose
E7D6C4
at pH 5.5 and 30C
20.1
cellobiose
E7D6C0
at pH 6.0 and 30C
21.8
cellobiose
-
at pH 5.0 and 30C
21.9
cellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
23
cellobiose
-
electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone
23.6
cellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
24
cellobiose
-
electron acceptor meldola blue
24
cellobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
25
cellobiose
B6D1P2
2,6-dichloroindophenol as electron acceptor
25.7
cellobiose
Q01738
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme
26
cellobiose
Q01738
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H
26
cellobiose
B6D1P2
cytochrome c as electron acceptor
27
cellobiose
-
; electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical
27.8
cellobiose
E7D6C1
at pH 5.0 and 30C
30
cellobiose
-
2,6-dichloroindophenpl or 1,4-benzoquinone as electron acceptor
34
cellobiose
-
electron acceptor cytochrome c from horseheart
37
cellobiose
-
electron acceptor K3Fe(CN)6
38.6
cellobiose
-
pH 4.0, 30C, wild-type enzyme
40
cellobiose
-
pH 4.0, 30C, recombinant enzyme
45.8
cellobiose
-
isoform CDH IIA, at pH 6.0 and 30C
53.1
cellobiose
-
at pH 5.5 and 30C
24.7
cellohexaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
1.23
Cellopentaose
-
in 50 mM MES buffer (pH 6.0), at 25C
9.31
Cellopentaose
-
-
10
Cellopentaose
-
-
10.2
Cellopentaose
-
isoform CDH IIB, at pH 6.0 and 30C
12
Cellopentaose
-
-
13.9
Cellopentaose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
14.7
Cellopentaose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
21.5
Cellopentaose
-
-
24
Cellopentaose
-
-
25.7
Cellopentaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
46.6
Cellopentaose
-
isoform CDH IIA, at pH 6.0 and 30C
1.23
Cellotetraose
-
in 50 mM MES buffer (pH 6.0), at 25C
2.7
Cellotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
6.1
Cellotetraose
-
at 25C
8.88
Cellotetraose
-
-
11.6
Cellotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
12
Cellotetraose
-
-
12
Cellotetraose
-
-
14.2
Cellotetraose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
15
Cellotetraose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
15.1
Cellotetraose
-
at 25C
20.7
Cellotetraose
-
-
23.9
Cellotetraose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
24
Cellotetraose
-
-
46
Cellotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
1.25
cellotriose
-
in 50 mM MES buffer (pH 6.0), at 25C
8.88
cellotriose
-
-
12
cellotriose
-
-
12
cellotriose
-
-
12.4
cellotriose
-
isoform CDH IIB, at pH 6.0 and 30C
24
cellotriose
-
-
24.78
cellotriose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
26
cellotriose
-
-
49.7
cellotriose
-
isoform CDH IIA, at pH 6.0 and 30C
0.4
cellulose
-
ferricyanide as electron acceptor
0.1
cytochrome c
Q01738
pH 4.5, reaction with cellobiose, mutant enzyme M65H
3.1
cytochrome c
-
pH 4.0, 20C
4.9
cytochrome c
-
at pH 5.0 and 30C
7.3
cytochrome c
-
at 25C
8.1
cytochrome c
-
pH 6.0, 20C
10.8
cytochrome c
Q01738
pH 4.5, reaction with cellobiose, wild-type enzyme
11.3
cytochrome c
-
pH 8.0, 20C
13
cytochrome c
-
-
16
cytochrome c
-
-
18.1
cytochrome c
-
at pH 5.0 and 30C
19.69
cytochrome c
-
-
20.5
cytochrome c
-
-
20.8
cytochrome c
-
at 45C
21.3
cytochrome c
-
at pH 5.0 and 30C
21.4
cytochrome c
-
at pH 7.5 and 30C
25
cytochrome c
-
-
25.2
cytochrome c
-
at pH 5.0 and 30C
27
cytochrome c
-
-
28.7
cytochrome c
-
at pH 6.0 and 30C
29.4
cytochrome c
-
pH 4.5, 30C, reaction with lactose
32.5
cytochrome c
-
pH 4.5, 30C, reaction with lactose
35.4
cytochrome c
-
pH 4.0, 30C, wild-type enzyme
37.3
cytochrome c
-
pH 4.0, 30C, recombinant enzyme
60
cytochrome c
B6D1P2
cellobiose as substrate, pH 3.5
1.5
D-galactose
-
isoform CDH IIA, at pH 6.0 and 30C
3.6
D-galactose
-
isoform CDH IIB, at pH 6.0 and 30C
0.5
D-glucose
-
at pH 5.0 and 30C
1.01
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 25C
1.31
D-glucose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
1.6
D-glucose
-
at pH 5.0 and 30C
1.9
D-glucose
-
at pH 6.0 and 30C
3.4
D-glucose
B6D1P2
cytochrome c as electron acceptor
3.8
D-glucose
B6D1P2
2,6-dichloroindophenol as electron acceptor
4.8
D-glucose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
7.1
D-glucose
-
at pH 5.0 and 30C
8.1
D-glucose
-
isoform CDH IIB, at pH 6.0 and 30C
10
D-glucose
-
at pH 7.5 and 30C
16.2
D-glucose
-
at pH 5.0 and 30C
16.8
D-glucose
E7D6C0
at pH 6.0 and 30C
18.4
D-glucose
-
at pH 5.0 and 30C
23.6
D-glucose
E7D6C1
at pH 5.0 and 30C
24.2
D-glucose
E7D6C4
at pH 5.5 and 30C
25.6
D-glucose
-
at pH 5.0 and 30C
37.7
D-glucose
-
at pH 5.5 and 30C
55
D-glucose
-
isoform CDH IIA, at pH 6.0 and 30C
4.3
D-mannose
-
isoform CDH IIB, at pH 6.0 and 30C
4.7
D-mannose
-
isoform CDH IIA, at pH 6.0 and 30C
0.33
D-xylose
-
in 50 mM MES buffer (pH 6.0), at 25C
1.5
D-xylose
-
isoform CDH IIA, at pH 6.0 and 30C
1.9
D-xylose
-
isoform CDH IIB, at pH 6.0 and 30C
74
FAD
-
reduction rate increases with increasing concententrations of the electron acceptors methyl-1,4-benzoquinone or Fe3+
58
ferricenium
B6D1P2
cellobiose as substrate, pH 3.5
34.7
ferricenium ion
-
pH 4.5, 30C, reaction with lactose
35.6
ferricenium ion
-
pH 4.5, 30C, reaction with lactose
5.5
ferricyanide
-
-
12.8
ferricyanide
-
at 25C
13.5
ferricyanide
-
-
14
ferricyanide
-
-
22
ferricyanide
-
-
24.1
ferricyanide
-
at 45C
31.36
ferricyanide
-
-
61
ferricyanide
B6D1P2
cellobiose as substrate, pH 3.5
1.4
Galactobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
2.1
galactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
1.92
glucose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
7.6
glucose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
27.8
K3Fe(CN)6
-
pH 4.5, 30C, reaction with lactose
36.9
K3Fe(CN)6
-
pH 4.5, 30C, reaction with lactose
0.003
lactose
-
pH 4.5, 23C, mutant enzyme H689Q or mutant H689A
0.005
lactose
-
pH 4.5, 23C, mutant enzyme N732D
0.3
lactose
-
pH 4.5, 23C, mutant enzyme N732E
0.7
lactose
-
pH 4.5, 23C, mutant enzyme N732A
1.2
lactose
-
pH 4.5, 23C, mutant enzyme N732Q
1.4
lactose
-
in 50 mM MES buffer (pH 6.0), at 25C
1.7
lactose
-
pH 4.5, 23C, mutant enzyme N732H
2 - 8
lactose
B6D1P2
2,6-dichloroindophenol as electron acceptor
2.3
lactose
-
at pH 5.0 and 30C
3.4
lactose
-
at pH 6.0 and 30C
3.9
lactose
-
at pH 5.0 and 30C
4.68
lactose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
5.9
lactose
-
at 25C
8.2
lactose
-
at pH 5.0 and 30C
9.2
lactose
-
at pH 5.0 and 30C
10.9
lactose
-
at pH 7.5 and 30C
11.1
lactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
11.8
lactose
-
isoform CDH IIB, at pH 6.0 and 30C
14
lactose
-
-
14
lactose
-
pH 6.0, 20C
14
lactose
-
-
14.3
lactose
-
pH 4.5, 23C, recombinant wild-type enzyme
16.8
lactose
-
at 45C
17.2
lactose
-
-
20.2
lactose
E7D6C4
at pH 5.5 and 30C
24.7
lactose
-
at pH 5.0 and 30C
25
lactose
-
at pH 5.0 and 30C
25.8
lactose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
26
lactose
-
-
26.9
lactose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
26.9
lactose
-
-
27
lactose
B6D1P2
cytochrome c as electron acceptor
28.9
lactose
E7D6C1
at pH 5.0 and 30C
33.7
lactose
E7D6C0
at pH 6.0 and 30C
49.3
lactose
-
isoform CDH IIA, at pH 6.0 and 30C
51.8
lactose
-
at pH 5.5 and 30C
0.17
maltose
-
in 50 mM MES buffer (pH 6.0), at 25C
0.2
maltose
-
at pH 5.0 and 30C
0.3
maltose
-
at pH 6.0 and 30C
0.315
maltose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
0.4
maltose
-
isoform CDH IIA, at pH 6.0 and 30C
0.51
maltose
E7D6C4
at pH 5.5 and 30C
0.6
maltose
-
at pH 5.5 and 30C
0.91
maltose
B6D1P2
cytochrome c as electron acceptor
1
maltose
-
at pH 5.0 and 30C
1.03
maltose
B6D1P2
2,6-dichloroindophenol as electron acceptor
1.2
maltose
-
-
1.2
maltose
-
at pH 5.0 and 30C
1.38
maltose
E7D6C1
at pH 5.0 and 30C
1.45
maltose
-
at pH 5.0 and 30C
1.8
maltose
-
at pH 5.0 and 30C
1.92
maltose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
2.08
maltose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
3.5
maltose
-
isoform CDH IIB, at pH 6.0 and 30C
3.6
maltose
-
at pH 5.0 and 30C
4
maltose
E7D6C0
at pH 6.0 and 30C
5
maltose
-
at pH 7.5 and 30C
20.5
maltose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
0.48
maltotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
1.4
maltotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
2.2
maltotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
1
maltotriose
-
isoform CDH IIB, at pH 6.0 and 30C
1.8
maltotriose
-
isoform CDH IIA, at pH 6.0 and 30C
2.1
mannobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
7.4
Mannopentaose
-
isoform CDH IIB, at pH 6.0 and 30C
19.8
Mannopentaose
-
isoform CDH IIA, at pH 6.0 and 30C
10.2
Mannose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
14
Mn3+-malonate
-
-
0.042
O2
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
0.19
O2
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
19.4
thiocellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
21.4
thiocellobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
27
thiocellobiose
-
-
17
triiodide ion
-
-
-
43.5
ubiquinone
-
pH 4.0, 30C, wild-type enzyme
44.2
ubiquinone
-
pH 4.0, 30C, recombinant enzyme
0.118
xylobiose
-
-
3
xylobiose
-
-
3.7
xylobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
5.8
xylobiose
-
isoform CDH IIB, at pH 6.0 and 30C
17.2
xylobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
18.3
xylobiose
-
pH 4.5, 30C, electron acceptor: 2,6-dichlorophenol indophenol
27
xylobiose
-
-
45.4
xylobiose
-
isoform CDH IIA, at pH 6.0 and 30C
4.6
xylose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
2.3
xylotriose
-
isoform CDH IIB, at pH 6.0 and 30C
6
xylotriose
-
isoform CDH IIA, at pH 6.0 and 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
185
1,4-benzoquinone
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
315
240
1,4-benzoquinone
A9XK88
mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
315
220
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
539
360
2,6-dichlorophenol indophenol
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
539
375
2,6-dichlorophenol indophenol
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
539
1700
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
539
1800
2,6-dichlorophenol indophenol
-
at pH 6.0 and 30C
539
2400
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
539
2900
2,6-dichlorophenol indophenol
-
at pH 5.5 and 30C
539
3900
2,6-dichlorophenol indophenol
-
at pH 5.0 and 30C
539
93
cellobiose
-
at pH 5.0 and 30C
82
117
cellobiose
E7D6C4
at pH 5.5 and 30C
82
117.1
cellobiose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
82
136
cellobiose
-
at pH 5.0 and 30C
82
222
cellobiose
E7D6C1
at pH 5.0 and 30C
82
279
cellobiose
E7D6C0
at pH 6.0 and 30C
82
320
cellobiose
-
in 50 mM MES buffer (pH 6.0), at 25C
82
320
cellobiose
-
at pH 5.0 and 30C
82
332
cellobiose
-
at pH 5.5 and 30C
82
450
cellobiose
-
at pH 6.0 and 30C
82
461.4
cellobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
82
508
cellobiose
-
isoform CDH IIA, at pH 6.0 and 30C
82
525
cellobiose
-
isoform CDH1, at pH 6.0 and 50C
82
527
cellobiose
-
isoform CDH IIB, at pH 6.0 and 30C
82
760
cellobiose
-
at pH 5.0 and 30C
82
850
cellobiose
-
at pH 5.0 and 30C
82
2193
cellobiose
-
isoform CDH2, at pH 6.0 and 50C
82
2700
cellobiose
-
at pH 5.0 and 30C
82
4000
cellobiose
-
at pH 7.5 and 30C
82
202.1
cellohexaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
1613
160
Cellopentaose
-
in 50 mM MES buffer (pH 6.0), at 25C
767
227
Cellopentaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
767
238
Cellopentaose
-
isoform CDH IIA, at pH 6.0 and 30C; isoform CDH IIB, at pH 6.0 and 30C
767
32.12
Cellotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
590
189
Cellotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
590
220
Cellotetraose
-
in 50 mM MES buffer (pH 6.0), at 25C
590
226
Cellotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
590
265
Cellotetraose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
590
150
cellotriose
-
in 50 mM MES buffer (pH 6.0), at 25C
516
216
cellotriose
-
isoform CDH IIA, at pH 6.0 and 30C
516
278
cellotriose
-
isoform CDH IIB, at pH 6.0 and 30C
516
305.6
cellotriose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
516
540
cytochrome c
-
at pH 6.0 and 30C
84
700
cytochrome c
-
at pH 5.0 and 30C
84
1000
cytochrome c
-
at pH 5.0 and 30C
84
3100
cytochrome c
-
at pH 7.5 and 30C
84
4400
cytochrome c
-
at pH 5.0 and 30C
84
0.007
D-glucose
-
at pH 5.0 and 30C
35
0.009
D-glucose
-
at pH 5.5 and 30C
35
0.011
D-glucose
-
at pH 6.0 and 30C
35
0.012
D-glucose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
35
0.014
D-glucose
E7D6C4
at pH 5.5 and 30C
35
0.014
D-glucose
-
isoform CDH IIB, at pH 6.0 and 30C
35
0.014
D-glucose
-
at pH 5.0 and 30C
35
0.015
D-glucose
-
isoform CDH IIA, at pH 6.0 and 30C
35
0.019
D-glucose
-
in 50 mM MES buffer (pH 6.0), at 25C
35
0.021
D-glucose
-
at pH 5.0 and 30C
35
0.048
D-glucose
-
at pH 7.5 and 30C
35
0.128
D-glucose
-
at pH 5.0 and 30C
35
0.195
D-glucose
E7D6C0
at pH 6.0 and 30C
35
0.215
D-glucose
-
at pH 5.0 and 30C
35
0.26
D-glucose
-
at pH 5.0 and 30C
35
0.3
D-glucose
E7D6C1
at pH 5.0 and 30C
35
0.0003
D-mannose
-
isoform CDH IIB, at pH 6.0 and 30C
216
0.0004
D-mannose
-
isoform CDH IIA, at pH 6.0 and 30C
216
0.00033
D-xylose
-
in 50 mM MES buffer (pH 6.0), at 25C
115
0.0005
D-xylose
-
isoform CDH IIA, at pH 6.0 and 30C
115
0.0012
D-xylose
-
isoform CDH IIB, at pH 6.0 and 30C
115
1.92
Galactobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
12535
0.0033
galactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
910
0.01
glucose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
223
52
lactose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
114
55.4
lactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
114
62
lactose
-
in 50 mM MES buffer (pH 6.0), at 25C
114
71
lactose
-
at pH 5.0 and 30C
114
109
lactose
-
at pH 5.0 and 30C
114
109
lactose
E7D6C4
at pH 5.5 and 30C
114
120
lactose
-
at pH 6.0 and 30C
114
124
lactose
-
at pH 5.0 and 30C
114
133
lactose
-
isoform CDH IIB, at pH 6.0 and 30C
114
134
lactose
E7D6C1
at pH 5.0 and 30C
114
155
lactose
E7D6C0
at pH 6.0 and 30C
114
159
lactose
-
at pH 5.5 and 30C
114
170
lactose
-
isoform CDH IIA, at pH 6.0 and 30C
114
190
lactose
-
at pH 5.0 and 30C
114
780
lactose
-
at pH 7.5 and 30C
114
3300
lactose
-
at pH 5.0 and 30C
114
0.004
maltose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
86
0.005
maltose
-
at pH 5.5 and 30C
86
0.009
maltose
-
at pH 5.0 and 30C
86
0.01
maltose
E7D6C4
at pH 5.5 and 30C
86
0.01
maltose
-
at pH 6.0 and 30C
86
0.023
maltose
-
isoform CDH IIA, at pH 6.0 and 30C
86
0.047
maltose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
86
0.067
maltose
-
at pH 5.0 and 30C
86
0.068
maltose
-
in 50 mM MES buffer (pH 6.0), at 25C
86
0.08
maltose
-
at pH 5.0 and 30C
86
0.09
maltose
-
at pH 5.0 and 30C
86
0.175
maltose
E7D6C1
at pH 5.0 and 30C
86
0.208
maltose
-
at pH 7.5 and 30C
86
0.26
maltose
E7D6C0
at pH 6.0 and 30C
86
0.82
maltose
-
at pH 5.0 and 30C
86
1.02
maltose
-
isoform CDH IIB, at pH 6.0 and 30C
86
450
maltose
-
at pH 5.0 and 30C
86
0.061
maltotetraose
-
isoform CDH IIA, at pH 6.0 and 30C
269
0.676
maltotetraose
-
in 100 mM sodium acetate buffer, pH 4.0, at 30C
269
2.74
maltotetraose
-
isoform CDH IIB, at pH 6.0 and 30C
269
0.053
maltotriose
-
isoform CDH IIA, at pH 6.0 and 30C
188
0.8
maltotriose
-
isoform CDH IIB, at pH 6.0 and 30C
188
1.39
mannobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
2026
0.506
Mannopentaose
-
isoform CDH IIA, at pH 6.0 and 30C
5180
0.88
Mannopentaose
-
isoform CDH IIB, at pH 6.0 and 30C
5180
0.002
Mannose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
988
1.1
O2
A9XK88
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30C
9
2.6
O2
A9XK88
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30C
9
1.33
xylobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
560
4.45
xylobiose
-
isoform CDH IIB, at pH 6.0 and 30C
560
12.8
xylobiose
-
isoform CDH IIA, at pH 6.0 and 30C
560
0.00091
xylose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30C
854
2
xylotriose
-
isoform CDH IIA, at pH 6.0 and 30C
1008
2.8
xylotriose
-
isoform CDH IIB, at pH 6.0 and 30C
1008
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.26
cellobiose
-
pH 6.0, 30C, substrate inhibition
0.363
cellobiose
-
pH 5.5, 30C, substrate inhibition
0.58
cellobiose
-
pH 5.0, 30C, substrate inhibition
0.8027
cellobiose
-
-
1.07
cellobiose
-
pH 4.5, 30C, substrate inhibition
1.33
cellobiose
-
pH 4.0, 30C, substrate inhibition
5.4
cellobiose
Coniophora puteana (Schum ex Fr) Karsten
-
-
125
cellobiose
B6D1P2
2,6-dichloroindophenol as electron acceptor
150
cellobiose
B6D1P2
cytochrome c as electron acceptor
0.195
cytochrome c
B6D1P2
cellobiose as substrate, pH 3.5
0.233
cytochrome c
-
mutant F166Y, cytochrome c reduction
2.51
cytochrome c
-
wild type, cellobiose oxidation with electron acceptors
3.54
cytochrome c
-
mutant F166Y, cellobiose oxidation with electron acceptors
31
lactobiono-1,5-lactone
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.08
-
purification step culture filtration
0.17
-
purification step ultra filtration
0.2
-
culture supernatant, in 100 mM sodium acetate buffer, pH 5.0, at 30C
0.28
-
isoform CDH2 from extracellular medium, at pH 6.0 and 50C
0.85
-
purified enzyme, with cellobiose as substrate, in 50 mM MES buffer (pH 6.0), at 25C
1.19
-
isoform CDH1 from extracellular medium, at pH 6.0 and 50C
1.3
-
purified mutant enzyme E279N, pH and temperature not specified in the publication
1.79
-
purification step POROS HP2
1.9
-
purified wild type enzyme, pH and temperature not specified in the publication
2.18
Coniophora puteana (Schum ex Fr) Karsten
-
-
2.2
E7D6C4
after 14.8fold purification, at pH 4.0 and 37C
2.2
A9XK88
culture supernatant, at pH 6.0 and 30C
2.64
-
purification step HiLoad Superdex-200
2.7
E7D6C4
after 14.8fold purification, at pH 4.5 and 37C
3.1
-
after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30C
5.3
-
at pH 4.0 and 25C
5.7
-
after 48.1fold purification, at pH 4.0 and 37C
7.1
E7D6C0
after 293fold purification, at pH 4.0 and 37C
7.4
-
HIC-column purified
7.4
-
after 21.2fold purification, at pH 4.0 and 37C
7.78
-
-
7.93
-
isoform CDH2 after 28.32fold purification, at pH 6.0 and 50C
8
A9XK88
after 3.6fold purification, at pH 6.0 and 30C
8.89
-
isoform CDH1 after 7.47fold purification, at pH 6.0 and 50C
8.9
-
after 7.3fold purification, at pH 4.0 and 37C
9
-
after 21.2fold purification, at pH 5.0 and 37C
10.3
-
after 48.1fold purification, at pH 5.5 and 37C
11.5
E7D6C0
after 293fold purification, at pH 6.0 and 37C
12.3
E7D6C1
after 26.3fold purification, at pH 4.0 and 37C
13.2
E7D6C1
after 26.3fold purification, at pH 5.5 and 37C
13.4
-
cytochrome c reduction at 60C
16.3
-
purification step Mono Q
17.8
-
after 7.3fold purification, at pH 5.0 and 37C
62
-
assay detects both intact and truncated enzyme
63
-
electron acceptor 2,6-dichloroindophenol
66
-
electron acceptor cytochrome c
69
-
assay detects both intact and truncated enzyme
additional information
Monilia sitophila
-
15.6 micromol/min*ml enzyme, electron acceptor phenol blue, enzyme activity in crude extracts of seven day old culture; 27.2 micromol/min*ml enzyme, electron acceptor 2,6-dichlorophenolindophenol, enzyme activity in crude extracts of seven day old culture; 4.9 micromol/min ml enzyme, electron acceptor potassium ferricyanide, enzyme activity in crude extracts of seven day old culture; 6.4 micromol/min ml enzyme, electron acceptor cytochrome c, enzyme activity in crude extracts of seven day old culture
additional information
-
1.7 micromol/min*l, cellulose bound enzyme after growth for 4 d; 6.8 micromol/min*l, enzyme activity in extracelluar solution after growth for 4 d
additional information
-
-
additional information
Q875J3
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.7 - 5
-
depends on electron acceptor
3 - 5
-
cellobiose + 2,6-dichlorophenol-indophenol, wild-type enzyme
3
-
cellobiose + cytochrome c, highest activity at pH 3, decreasing as the pH increases, recombinant wild-type enzyme, mutant enzymes N732H, N732Q and N732A
3.5 - 4.5
-
acceptor: methylene blue
3.5 - 4.5
-
lactose + cytochrome c, in acetate buffer
3.5
-
lactose + cytochrome c, in acetate buffer
3.5
Q875J3
two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c
3.5
B6D1P2
lactose as substrate, 1,4-benzoquinone or ferricenium or cytochrome c as electron acceptor
4 - 4.5
Monilia sitophila
-
-
4 - 4.5
-
the optimum pH range for direct electron transfer between enzyme and electrode is pH 4.0-4.5
4
-
methylen blue as electron acceptor
4
Q875J3
two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone
4
Q7Z975
-
4
-
activity assay
4
-
enzyme treatment of methyl beta-D-glucopyranoside, monosaccharides and cellulose
4
-
electron acceptor: 2,6-dichloroindophenol
4.5 - 5
-
depending on the electron acceptor
4.5 - 7.5
Q875J3
two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c
4.5
-
lactose + 2,6-dichlorophenol indophenol or cytochrome c, in citrate buffer
4.5
-
lactose + cytochrome c, in citrate buffer
4.5
Q6AW20
-
4.5
Q01738
review, different references
4.5
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor
4.5
B6D1P2
lactose as substrate, 2,6-dichloroindophenol as electron acceptor
4.5
-
electron acceptor: 2,6-dichloroindophenol
5 - 6
-
acceptor: 2,6-dichlorophenolindophenol
5 - 6
-
the optimum pH range for mediated electron transfer between enzyme and electrode is pH 5.0-6.0
5
-
lactose + 2,6-dichlorophenol indophenol, in citrate buffer
5
-
cellobiose + cytochrome c, mutant enzyme N732E
5
Q875J3
two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone
5
-
activity assay
5
Q01738
review, different references
5
-
lactose as electron donor, cytochrome c as electron acceptor
5
-
electron acceptor: 2,6-dichloroindophenol
5
-
using 2,6-dichlorophenolindol as electron donor
5.5 - 7
-
reaction with 2,6-dichlorophenol indophenol
6
-
reaction with cytochrome c
6
-
cellobiose + cytochrome c, mutant enzyme N732D
6
Chaetomium sp.
-
-
6
Q01738
review, different references
6
Chaetomium sp.
-
-
6
-
electron acceptor: 2,6-dichloroindophenol
6.5 - 7
-
cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732E and N732D
6.5
-
cyclic voltametry
7 - 9
-
cytochrome c as electron acceptor
7
-
acceptor: 3,5-di-tert-butyl-1,2-benzoquinone
7
-
2,6-dichlorophenolindophenol as electron acceptor; 3,5-di-tert-butyl-1,2-benzoquinone as electron acceptor
7
Q9P8H5
review, different references
7
-
using 1,4-benzoquinone as electron donor
7.5
Q9P8H5
review, different references
8 - 9
-
acceptor: phenazine methosulfate
9
-
ferricyanide as electron acceptor
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.5 - 4.5
B6D1P2
lactose as substrate, cytochrome c as electron acceptor
2.5 - 6.5
-
pH 2.5: about 40% of maximal activity, pH 6.5: about 60% of maximal activity
2.5 - 6.5
B6D1P2
lactose as substrate, 2,6-dichloroindophenol or 1,4-benzoquinone or ferricenium as electron acceptor
3 - 5
-
pH 3.0: about 70% of maximal activity, pH 5.0: about 40% of maximal activity, lactose + cytochrome c, in acetate buffer
3 - 5
-
pH 3.0: about 60% of maximal activity, pH 5.0: about 70% of maximal activity, lactose + cytochrome c, in acetate buffer
3 - 5.5
-
highest activity at pH 3, decreases to 10-20% of the activity when the pH increases to pH 5.5, cellobiose + cytochrome c recombinant wild-type enzyme, mutant enzymes N732H, N732Q and N732A
3 - 7
-
highest activity at pH 3, decreases when the pH increases to pH 7.0, cellobiose + 2,6-dichlorophenol-indophenol, to about 10% with recombinant wild-type enzyme, to about 25% with mutant enzymes N732Q, to about 40% with mutant enzyme N732A and to about 50% with mutant enzyme N732H
3.2 - 5.3
Q875J3
pH 3.2: about 50% of maximal activity, pH 5.3: about 45% of maximal activity, reaction with cellobiose and cytochrome c
3.5 - 5.5
-
pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer
3.5 - 5.5
-
pH 3.5: about 50% of maximal activity, pH 5.5: about 20% of maximal activity, lactose + cytochrome c, in citrate buffer
3.5 - 6
-
the CDH activity decreases very sharply below pH 4.5. The recombinant enzyme remains at 60.8% of the maximum CDH activity at pH 4.0, and at only 22.6% CDH activity at pH 3.5
3.7 - 8
-
more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor
3.8 - 6.5
-
more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor
4 - 6
-
pH 4.0: about 70% of maximal activity, pH 6.0: about 40% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer
4 - 6
-
pH 4.0: about 75% of maximal activity, pH 6.0: about 65% of maximal activity, lactose + 2,6-dichlorophenol indophenol, in citrate buffer
4 - 6
-
pH 5.0: about 35% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732E
4 - 6
-
below pH 4.0, and particularly above pH 6.0, the activity is observed to drop (65% activity at pH 3.0, 45% activity at pH 7.0 and no activity at pH 9.0)
4 - 7
-
at 40C
4 - 7
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor
4.1 - 5.7
-
pH 4.1: less than 50% of maximum activity, pH 5.7: less than 50% of maximum activity
4.5 - 8
-
pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with 2,6-dichlorophenol indophenol
5 - 7
-
pH 5.0: about 40% of maximal activity, pH 7.0: about 20% of maximal activity, cellobiose + cytochrome c, mutant enzyme N732D
5 - 7.5
-
about 50% activity between pH 5.0 and 7.5
5.5 - 6
-
the mediated electron transfer currents increase with pH and are 2.3 times lower at pH 5.5 and 6.0 for CDH adsorbed on electrosynthesised polyaniline modified electrode compared to CDH on plain screen printed electrodes
5.5 - 7.5
-
pH 5.0: about 45% of maximal activity, pH 7.0: about 65% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732E
5.5 - 8
-
pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, cellobiose + 2,6-dichlorophenol-indophenol, mutant enzyme N732D
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
Chaetomium sp.
-
-
25
Q7Z975
-
25
Q01738
review, different references
30
Q6AW20
-
30
-
activity assay
30
-
enzyme treatment of methyl beta-D-glucopyranoside, monosaccharides and cellulose
35
Q01738
review, different references
35
B6D1P2
broad optimum: 30C-40C, lactose as substrate, cytochrome c as electron acceptor, pH 3.5
40
-
activity assay
50
Q875J3
reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone
60
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor
60
B6D1P2
lactose as substrate, 2,6-dichloroindophenol as electron acceptor, pH 4.5
65
-
sharp drop above
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 60
B6D1P2
lactose as substrate, cytochrome c as electron acceptor, pH 3.5 (analysis starts at 20C)
20 - 70
B6D1P2
lactose as substrate, 2,6-dichloroindophenol as electron acceptor, pH 4.5 (analysis starts at 20C)
30 - 60
Q875J3
30C: about 70% of maximal activity, 50C: about 80% of maximal activity, 60C: 30% of maximal activity with wild-type enzyme, 25% of maximal activity with recombinant enzyme, reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone
30 - 80
-
30C: about 75% of maximal activity, 80C: about 55% of maximal activity
45 - 55
-
about 70% activity between 45 and 55C
60 - 70
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3
B6D1P2
one of the two glycoforms, calculated: 4.2
3.1
B6D1P2
one of the two glycoforms, calculated: 4.2
3.42
-
heme domain
3.8
-
isoelectric focusing
4.1
E7D6C0
isoelectric focusing
4.2
Q7Z975
-
4.2
E7D6C1
isoelectric focusing
4.3
Q8J2T4
-
4.3
-
isoelectric focusing
4.3
E7D6C4
isoelectric focusing
4.4
-
isoelectric focusing
4.6