Information on EC 1.1.3.6 - cholesterol oxidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.3.6
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RECOMMENDED NAME
GeneOntology No.
cholesterol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cholesterol + O2 = cholest-5-en-3-one + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
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radical or hydride transfer mechanism
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cholesterol degradation to androstenedione I (cholesterol oxidase)
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Steroid degradation
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SYSTEMATIC NAME
IUBMB Comments
cholesterol:oxygen oxidoreductase
Contains FAD. Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyse the first two steps in the degradation of cholesterol. The enzyme catalyses the oxidation of the 3beta-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Delta5 position to Delta6 position (cf. EC 5.3.3.1, steroid Delta-isomerase).
CAS REGISTRY NUMBER
COMMENTARY hide
9028-76-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SFF34
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Manually annotated by BRENDA team
SFF34
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Manually annotated by BRENDA team
isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China
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Manually annotated by BRENDA team
isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China
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-
Manually annotated by BRENDA team
Brevibacterium sp. CCTCC M201008
CCTCC M201008
SwissProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Burkholderia xenovorans
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UniProt
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Corynebacterium cholesterolicum
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Nocardia erythropolis
-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Nocardia rhodochrous
strain F2, gene choF
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ST-200
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Manually annotated by BRENDA team
Pseudomonas spp.
-
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Manually annotated by BRENDA team
Rhodococcus equi ATCC33706
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UniProt
Manually annotated by BRENDA team
Rhodococcus sp. CECT3014
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UniProt
Manually annotated by BRENDA team
solated from soil
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Manually annotated by BRENDA team
fragment
SwissProt
Manually annotated by BRENDA team
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India)
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-
Manually annotated by BRENDA team
from soil sample collected from effluent sediment of a steel plant in Barabanki (Uttar Pradesh, India)
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Manually annotated by BRENDA team
strain A19249
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Streptoverticillium cholesterolieum
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Manually annotated by BRENDA team
Streptoverticillum cholesterolicum
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-androstadiene-3,17-dione + H2O
?
show the reaction diagram
-
11% of the activity with cholesterol, enzyme CO1
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?
1-propanol + O2
propanal + H2O2
show the reaction diagram
-
-
-
?
17beta-hydroxymethyl-5-androsten-3beta-ol + O2
17beta-hydroxymethyl-5-androsten-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
20,22-dihydroxycholesterol + O2
20,22-dihydroxycholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
20,25-diazacholesterol + O2
20,25-diazacholest-4-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
20-hydroxycholesterol + O2
20-hydroxycholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
22-hydroxycholesterol + O2
22-hydroxycholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
23,24-dinor-5-cholen-3beta,22-diol + O2
23,24-dinor-5-cholen-22-ol-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
24-hydroxycholesterol + O2
24-hydroxycholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
24-oxocholesterol + O2
24-oxocholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
25-hydroxy-27-norcholesterol + O2
?
show the reaction diagram
Nocardia erythropolis
-
-
-
-
?
25-hydroxycholesterol + O2
25-hydroxycholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
26-hydroxycholesterol + O2
26-hydroxycholest-5-en-3-one + H2O2
show the reaction diagram
3beta-chlorocholest-5-ene + O2
? + H2O2
show the reaction diagram
3beta-hydroxyandrost-5-en-17-one + O2
androst-5-en-3,17-dione + H2O2
show the reaction diagram
3beta-hydroxypregn-5-en-20-one + O2
pregn-5-en-3,20-dione + H2O2
show the reaction diagram
4,4-dimethylcholesterol + O2
4,4-dimethylcholest-5-en-3-one
show the reaction diagram
Nocardia erythropolis
-
-
-
?
4alpha-methylcholesterol + O2
4alpha-methylcholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
4beta-methylcholesterol + O2
4beta-methylcholest-5-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
5,7-cholestadien-3beta-ol + O2
5,7-cholestadien-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
5-androstene-3beta,17beta-diol + O2
5-androsten-17beta-ol-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
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?
5-cholene-3beta,24-diol + O2
5-cholen-24-ol-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
5-pregnen-3beta,20beta-diol + O2
5-pregnen-20beta-ol-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
5alpha-androstan-3-ol-17-one + O2
5alpha-androstan-3,17-dione + H2O2
show the reaction diagram
-
15% of the activity with cholesterol
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?
5alpha-cholest-8(14)-en-3beta-ol + O2
5alpha-cholest-8(14)-en-3-one + H2O2
show the reaction diagram
Nocardia erythropolis
-
-
-
?
5alpha-cholestan-3beta-ol + O2
5alpha-cholestan-3-one + H2O2
show the reaction diagram
5alpha-cholestane + O2
?
show the reaction diagram
5alpha-cholestane-3beta-ol-7-one + O2
5alpha-cholestane-3,7,dione
show the reaction diagram
7-dehydrocholesterol + O2
cholest-4,7-dien-3-one + H2O2
show the reaction diagram
7-keto-cholesterol + O2
?
show the reaction diagram
7alpha-hydroxy-cholesterol + O2
7alpha-hydroxy-cholest-4-en-3-one + H2O2
show the reaction diagram
7beta-hydroxy-cholesterol + O2
7beta-hydroxy-cholest-4-en-3-one + H2O2
show the reaction diagram
7E,22-ergostadien-3beta-ol + O2
7E,22-ergostadien-3-one + H2O2
show the reaction diagram
androst-4-ene-3,17-dione + O2
? + H2O2
show the reaction diagram
-
-
-
?
beta-cholestanol + O2
?
show the reaction diagram
beta-sitosterol + O2
?
show the reaction diagram
beta-sitosterol + O2
? + H2O2
show the reaction diagram
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
show the reaction diagram
beta-stigmasterol + O2
?
show the reaction diagram
-
36% of the activity with cholesterol
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?
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
show the reaction diagram
campesterol + O2
(24R)-ergost-5-en-3-one + H2O2
show the reaction diagram
cholest-4-en-3-one + O2
? + H2O2
show the reaction diagram
-
-
-
?
cholest-5-en-3beta-ol 7-one + O2
cholest-5-en-3,17-dione + H2O2
show the reaction diagram
-
low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
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?
cholestanol + O2
?
show the reaction diagram
cholestenone + O2
?
show the reaction diagram
cholesterol + O2
6beta-hydroperoxycholest-4-en-3-one + H2O2
show the reaction diagram
cholesterol + O2
cholest-4-en-3-one + H2O2
show the reaction diagram
cholesterol + O2
cholest-5-en-3-one + H2O2
show the reaction diagram
cholesterol-5alpha,6beta-epoxide + O2
?
show the reaction diagram
coprostane + O2
?
show the reaction diagram
cyclohexanol + O2
cyclohexanal + H2O2
show the reaction diagram
-
-
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?
dehydro-epi-androsterone + O2
?
show the reaction diagram
dehydroandrosterone + O2
? + H2O2
show the reaction diagram
-
-
-
?
dehydroepiandrosterone + O2
?
show the reaction diagram
-
27% of the activity with cholesterol
-
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?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O
show the reaction diagram
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
show the reaction diagram
dehydroisoandrosterone + O2
androst-5-en-3,17-dione + H2O
show the reaction diagram
desmosterol + O2
cholesta-5,24-dien-3-one + H2O2
show the reaction diagram
dihydrocholesterol + O2
5alpha-cholestan-3-one + H2O2
show the reaction diagram
diosgenin + O2
? + H2O2
show the reaction diagram
-
-
-
?
diosgenin + O2
diosgenone + H2O2
show the reaction diagram
epiandosterone + O2
?
show the reaction diagram
epiandrosterone + O2
?
show the reaction diagram
ergosta-5,7,22-trien-3beta-ol + O2
ergostan-5,7,22-trien-3-one + H2O2
show the reaction diagram
-
18% of the activity with cholesterol
-
-
?
ergosterol + O2
?
show the reaction diagram
ergosterol + O2
dehydroergosterol + H2O2
show the reaction diagram
estrone + O2
estra-1,5(10)-dien-3,17-dione + H2O2
show the reaction diagram
-
low activity with wild-type enzyme, no activity with mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T
-
-
?
ethanol + O2
ethanal + H2O2
show the reaction diagram
-
-
-
?
fucosterol + O2
stigmasta-5,24(28)-dien-3-one + H2O2
show the reaction diagram
hecogenin + O2
5alpha-(25R)-spirostan-3,12-dione + H2O2
show the reaction diagram
lanosterol + O2
5alpha-lanosta-8,24-dien-3-one
show the reaction diagram
lanosterol + O2
lanosta-8,24-dien-3-one + H2O2
show the reaction diagram
-
slow rate
-
?
methanol + O2
methanal + H2O2
show the reaction diagram
-
-
-
?
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
show the reaction diagram
pregnenolone + O2
?
show the reaction diagram
pregnenolone + O2
? + H2O2
show the reaction diagram
-
-
-
?
sitost-5-en-3beta-ol + O2
sitost-5-en-3-one + H2O2
show the reaction diagram
-
88% of the activity with cholesterol
-
-
?
sitosterol + O2
?
show the reaction diagram
stigmast-5-en-3beta-ol + O2
stigmast-5-en-3-one + H2O2
show the reaction diagram
-
68% of the activity with cholesterol
-
-
?
stigmastanol + O2
stigmasta-5,22t-dien-3-one + H2O2
show the reaction diagram
stigmastanol + O2
stigmastanone + H2O2
show the reaction diagram
-
-
-
?
stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
show the reaction diagram
trans-androsterone + O2
?
show the reaction diagram
trans-dehydroandrosterone + O2
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3beta-chlorocholest-5-ene + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
low activity
-
-
?
androst-4-ene-3,17-dione + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
beta-cholestanol + O2
?
show the reaction diagram
beta-sitosterol + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
beta-sitosterol + O2
stigmast-5-en-3-one + H2O2
show the reaction diagram
beta-stigmasterol + O2
stigmasta-5,22-dien-3-one + H2O2
show the reaction diagram
cholest-4-en-3-one + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
cholesterol + O2
cholest-4-en-3-one + H2O2
show the reaction diagram
cholesterol + O2
cholest-5-en-3-one + H2O2
show the reaction diagram
dehydroandrosterone + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
dehydroepiandrosterone + O2
androst-5-en-3,17-dione + H2O2
show the reaction diagram
diosgenin + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
epiandosterone + O2
?
show the reaction diagram
ergosterol + O2
dehydroergosterol + H2O2
show the reaction diagram
pregnenolone + O2
5-pregnen-3,20-dione + H2O2
show the reaction diagram
pregnenolone + O2
? + H2O2
show the reaction diagram
H9NJ43, H9NJ52
-
-
-
?
additional information
?
-