Information on EC 3.2.1.6 - endo-1,3(4)-beta-glucanase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.6
-
RECOMMENDED NAME
GeneOntology No.
endo-1,3(4)-beta-glucanase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3
show the reaction diagram
-
-
-
-
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3
show the reaction diagram
reaction is not in rapid equilibrium
-
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3
show the reaction diagram
substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase
-
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3
show the reaction diagram
substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase
Talaromyces emersonii CBS 814.70
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3(or 4)-beta-D-glucan 3(4)-glucanohydrolase
Substrates include laminarin, lichenin and cereal D-glucans; different from EC 3.2.1.52 beta-N-acetylhexosaminidase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,3-(4)-beta-glucanase (endoglucanase)
-
-
1,3-1,4-beta-glucanase EII (barley)
-
-
1,3-beta-D-glucanases
-
-
Agl9A
D7PC10
-
Agl9A
D7PC10
-
-
AkLam36
-
-
beta-1,3-1,4-glucanase
-
-
beta-1,3-1,4-glucanase
Q8GMY0
-
beta-1,3-1,4-glucanase
Bacillus licheniformis EGW039
-
-
-
beta-1,3-1,4-glucanase
-
-
beta-1,3-1,4-glucanase
A8CGP1
-
beta-1,3-1,4-glucanase
Bacillus subtilis MA139
A8CGP1
-
-
beta-1,3-1,4-glucanase
Q55365
-
beta-1,3-1,4-glucanase
-
-
beta-1,3-1,4-glucanase
Talaromyces emersonii CBS 814.70
-
-
-
beta-1,3-glucanase
-
-
beta-1,3-glucanase
-
-
beta-1,3:1,4 glucanase
-
-
beta-glucanase
Saccharomyces lactis
-
-
beta-glucanase
-
-
bglBC1
Bacillus circulans ATCC21367
-
-
-
enda-beta-(1-3),(1-4)-glucanase
-
-
enda-beta-(1-3),(1-4)-glucanase
Bacillus circulans ATCC21367
-
-
-
endo beta-1,3-glucanase IV
Flavobacterium dormitator
-
-
endo-1,3(4)-beta-D-glucanase
Flavobacterium dormitator
-
-
endo-1,3(4)-beta-glucanase
Q874E3
-
endo-1,3(4)-beta-glucanase
-
-
endo-1,3-1,4-beta-D-glucanase
-
-
endo-1,3-1,4-beta-D-glucanase
Talaromyces emersonii CBS 814.70
-
-
-
endo-1,3-beta-D-glucanase
-
-
endo-1,3-beta-D-glucanase
Talaromyces emersonii CBS 814.70
-
-
-
endo-1,3-beta-glucanase
-
-
endo-1,3-beta-glucanase
Cherax destructor Clark
-
-
-
endo-1,3-beta-glucanase
-
-
endo-1,3-beta-glucanase
-
-
endo-1,3-beta-glucanase
Q000P7
-
endo-1,3-beta-glucanase
Q000P7
-
-
endo-1,3-beta-glucanase
-
-
endo-beta-(1-3)-D-glucanase
-
-
endo-beta-(1-3)-D-glucanase
-
-
endo-beta-1,3(4)-glucanase
-
-
endo-beta-1,3(4)-glucanase
Phaffia rhodozyma CBS 6938
-
-
-
endo-beta-1,3-1,4-D-glucanase
-
-
endo-beta-1,3-1,4-glucanase
-
-
endo-beta-1,3-1,4-glucanase
Bacillus subtilis BF7658
-
-
-
endo-beta-1,3-glucanase
Flavobacterium dormitator
-
-
endo-beta-1,3-glucanase
-
-
endo-beta-1,3-glucanase
-
-
Fsbeta-glucanase
-
-
H(A16-M)
-
1,3-1,4-beta-glucanase from Bacillus macerans in which the 16 N-terminal residues have been replaced by the respective residues of the enzyme from Bacillus amyloliquefaciens
LamA
Q000P7
-
lamarinase
-
-
lamarinase
Cherax destructor Clark
-
-
-
laminaranase
-
-
laminaranase
Talaromyces emersonii CBS 814.70
-
-
-
laminarinase
-
-
laminarinase
-
-
laminarinase
-
-
laminarinase
-
-
laminarinase
Q000P7
-
laminarinase
Q000P7
-
-
laminarinase
-
-
laminarinase 16A
Q874E3
-
laminarinase II
-
-
laminarinase Lam16A
-
-
nonlytic endo-beta-1,3-glucanase I
Flavobacterium dormitator
-
-
Xyn10B
-
modular enzyme composed of two family-22 carbohydrate-binding modules, a family-9 carbohydrate-binding module, and two S-layer homologous modules
Xyn10B
-
modular enzyme comprising two family-22 carbohydrate-binding modules (CBMs), a family-10 catalytic module of glycoside hydrolases, a family-9 carbohydrate-binding module, and two S-layer homologous modules consecutively from the N-terminus
additional information
A8CGP1
the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif
additional information
Bacillus subtilis MA139
A8CGP1
the bacterial beta-1,3-1,4-glucanase belongs to the glycosyl hydrolase family 16, GH16, possessing the conserved EIDIEF motif
-
additional information
-
HdLam33 belongs to the glycoside hydrolase family 16, GH16
additional information
-
LamR belongs to glycosyl hydrolase family 16
additional information
-
the enzyme belongs to the glycosyl hydrolase family 81, GH81
CAS REGISTRY NUMBER
COMMENTARY
62213-14-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
D7PC10
UniProt
Manually annotated by BRENDA team
-
D7PC10
UniProt
Manually annotated by BRENDA team
ATCC21367
-
-
Manually annotated by BRENDA team
Bacillus circulans ATCC21367
ATCC21367
-
-
Manually annotated by BRENDA team
strain EGW039
-
-
Manually annotated by BRENDA team
Bacillus licheniformis EGW039
strain EGW039
-
-
Manually annotated by BRENDA team
mutant strain ZJF-1A5
-
-
Manually annotated by BRENDA team
strain BF7658, gene gls
-
-
Manually annotated by BRENDA team
strain MA139, isolated from pig intestine
UniProt
Manually annotated by BRENDA team
Bacillus subtilis BF7658
strain BF7658, gene gls
-
-
Manually annotated by BRENDA team
Bacillus subtilis MA139
strain MA139, isolated from pig intestine
UniProt
Manually annotated by BRENDA team
strain Clark 1936
-
-
Manually annotated by BRENDA team
Cherax destructor Clark
strain Clark 1936
-
-
Manually annotated by BRENDA team
Flavobacterium dormitator
var. glucanolyticae FA-5
-
-
Manually annotated by BRENDA team
Cytophaga
-
-
Manually annotated by BRENDA team
Polyporus tulipiferae
-
-
Manually annotated by BRENDA team
shiitake mushroom
-
-
Manually annotated by BRENDA team
H(A16-M), a 1,3-1,4-beta-glucanase from Bacillus macerans in which the 16 N-terminal residues have been replaced by the respective residues of the enzyme from Bacillus amyloliquefaciens
-
-
Manually annotated by BRENDA team
strain CCRC 17245
UniProt
Manually annotated by BRENDA team
strain CCRC 17245
UniProt
Manually annotated by BRENDA team
adult, female
-
-
Manually annotated by BRENDA team
CBS 6938
-
-
Manually annotated by BRENDA team
Phaffia rhodozyma CBS 6938
CBS 6938
-
-
Manually annotated by BRENDA team
DSM 1330
-
-
Manually annotated by BRENDA team
member of glycosyl hydrolase family 16
UniProt
Manually annotated by BRENDA team
Saccharomyces lactis
-
-
-
Manually annotated by BRENDA team
several strains, overview
-
-
Manually annotated by BRENDA team
Streptococcus bovis JB1
-
-
-
Manually annotated by BRENDA team
strain CBS 814.70, two endo-beta-D-glucanases EG VI and EG VII
-
-
Manually annotated by BRENDA team
Talaromyces emersonii CBS 814.70
strain CBS 814.70, two endo-beta-D-glucanases EG VI and EG VII
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
D7PC10
enzyme is highly resistant to trypsin, alpha-chymotrypsin, collagenase and Neutrase 0.8L
metabolism
-
enzyme is highly resistant to trypsin, alpha-chymotrypsin, collagenase and Neutrase 0.8L
-
physiological function
-
When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3-D structure, at 1.5 A resolution, of CtLic26A in complex with thiopentasaccharide. Crystals are in space group P2(1)2(1)2(1) with cell dimensions of a = 49.3 A, b = 63.0 A and c = 78.3 A and with a single molecule of CtLic26A in the asymmetric unit
-
crystal structure of truncated Fsbeta-glucanase in complex with beta-1,3-1,4-cellotriose, resolution of 2.3 A
-
hanging-drop vapor-diffusion at room temperature, space group P2(1)2(1)2(1), cell parameters: a = 75.77 A, b = 88.76 A, c = 154.79 A. High-resolution crystal structure of the hybrid 1,3-1,4-beta-glucanase H(A16-M)E105Q/E109Q in complex with a beta-glucan tetrasaccharide
-
structure was solved at 1.34 A resolution using native sulfur SAD X-ray crystallography. Crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 38, b = 47, c = 152 A
-
X-ray crystallography screening of ligands bound nucleophile mutant E115S, to 1 A resolution. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. alpha-Laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima
-
to 1.95 A resolution, and molecular dynamics simulation studies. LamR is formed mostly by beta-sheets in a complex jelly roll topology that is conserved among members of glycosyl hydrolase family 16. It contains a relatively large number of salt bridges, which are not randomly distributed on the structure. They form clusters interconnecting beta-sheets of the catalytic domain. Structure reveals a glycerol molecule fortuitously bound to the active site of the enzyme molecule
O52754
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ethanol
-
40% of activity after 20 min incubation with 10% v/v ethanol
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, 0.1 M McIlvaine buffer, pH 5.8, retains almost all initial activity after 1 week of storage
D7PC10
0°C, 7 days, no detectable loss of activity
-
-20°C, cell paste, without appreciable loss of activity
Saccharomyces lactis
-