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Literature summary for 3.2.1.6 extracted from

  • Ekinci, M.S.; McCrae, S.I.; Flint, H.J.
    Isolation and overexpression of a gene encoding an extracellular beta-(1,3-1,4)-glucanase from Streptococcus bovis JB1 (1997), Appl. Environ. Microbiol., 63, 3752-3756.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, Enterococcus faecalis, Lactococcus lactis Streptococcus equinus JB1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Streptococcus equinus JB1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptococcus equinus JB1
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24100
-
-
 Streptococcus equinus JB1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucans + H2O Streptococcus equinus JB1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus equinus JB1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus equinus JB1

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Streptococcus equinus JB1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.4
-
 laminarin as substrate Streptococcus equinus JB1
303.5
-
 lichenin as substrate Streptococcus equinus JB1
338.3
-
 barley beta-glucan as substrate Streptococcus equinus JB1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucans + H2O
-
 Streptococcus equinus JB1 ?
-
 ?
beta-glucan + H2O beta-(1-3)-beta-(1-4)mixed-linked, from barley Streptococcus equinus JB1 laminaridextrins
-
 ?
laminarin + H2O
-
 Streptococcus equinus JB1 D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose reducing sugars ?
lichenin + H2O
-
 Streptococcus equinus JB1 beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 stable for 20 min at 50°C, at 60°C loss of 75% of maximal activity Streptococcus equinus JB1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 in 50 mM sodium phosphate buffer Streptococcus equinus JB1

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 at pH 5 69%, at pH 4 23%, at pH 3 2% of maximal activity Streptococcus equinus JB1