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Literature summary for 3.2.1.6 extracted from
- Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor (2008), J. Exp. Biol., 211, 2275-2287.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE, a second band of 62000 Da is detected | Cherax destructor |
| 62000 | - |
gel filtration chromatography | Cherax destructor |
| 62000 | - |
SDS-PAGE, a second band of 41000 Da is detected, the larger band may either be a minor impurity or represent a native laminarinase dimer | Cherax destructor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cherax destructor | - |
- |
- |
| Cherax destructor Clark 1936 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Cherax destructor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | - |
Cherax destructor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
homogenate | Cherax destructor |
| 1.32 | - |
DEAE anion exchange chromatography | Cherax destructor |
| 6.37 | - |
HIC chromatography | Cherax destructor |
| 18.31 | - |
gel filtration chromatography | Cherax destructor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Cherax destructor | D-glucose + laminarioligosaccharides | - |
? |  |
| laminarin + H2O | - |
Cherax destructor Clark 1936 | D-glucose + laminarioligosaccharides | - |
? | |
| additional information | laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds | Cherax destructor | ? | - |
? | |
| additional information | laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds | Cherax destructor Clark 1936 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,3-beta-glucanase | - |
Cherax destructor |
| lamarinase | - |
Cherax destructor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Cherax destructor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Cherax destructor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Cherax destructor |
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