Literature summary for 3.2.1.6 extracted from

Vasur, J.; Kawai, R.; Jonsson, K.H.; Widmalm, G.; Engstroem, A.; Frank, M.; Andersson, E.; Hansson, H.; Forsberg, Z.; Igarashi, K.; Samejima, M.; Sandgren, M.; Stahlberg, J.
Synthesis of cyclic beta-glucan using laminarinase 16A glycosynthase mutant from the basidiomycete Phanerochaete chrysosporium (2010), J. Am. Chem. Soc., 132, 1724-1730.

Crystallization (Commentary)

Crystallization (Comment)	Organism
X-ray crystallography screening of ligands bound nucleophile mutant E115S, to 1 A resolution. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. alpha-Laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima	Phanerodontia chrysosporium

Crystallization (Comment)

Organism

X-ray crystallography screening of ligands bound nucleophile mutant E115S, to 1 A resolution. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. alpha-Laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima

Phanerodontia chrysosporium

Protein Variants

Protein Variants	Comment	Organism
E115S	X-ray crystallography screening of ligands bound nucleophile mutant E115S. Laminariheptaose binds in an arch with the reducing and nonreducing ends occupying either side of the catalytic cleft of the enzyme. R-laminariheptaosyl fluoride can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan	Phanerodontia chrysosporium

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	Q874E3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-laminariheptaosyl fluoride	-	Phanerodontia chrysosporium	?	substrate can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. NMR confirms uniquely beta-1,3-linkages and no reducing end. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima	?
laminariheptaose + H2O	-	Phanerodontia chrysosporium	?	-	?

Synonyms

Synonyms	Comment	Organism
laminarinase 16A	-	Phanerodontia chrysosporium