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Literature summary for 3.2.1.6 extracted from
- Enzymatic properties and the primary structure of a beta-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai (2009), Comp. Biochem. Physiol. B, 154, 113-120.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| HdLam33, DNA and amino acid sequence determination and analysis | Haliotis discus hannai |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | digestive fluid | Haliotis discus hannai | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Haliotis discus hannai |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | Haliotis discus hannai | - |
laminaribiose + D-glucose | - |
? |  |
| laminaritetraose + 2 H2O | Haliotis discus hannai | - |
laminaribiose + 2 D-glucose | - |
? | |
| additional information | Haliotis discus hannai | HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haliotis discus hannai | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration to homogeneity | Haliotis discus hannai |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive juice | - |
Haliotis discus hannai | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 68 | - |
purified native enzyme | Haliotis discus hannai |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Haliotis discus hannai | laminaribiose + D-glucose | - |
? | |
| laminaritetraose + 2 H2O | - |
Haliotis discus hannai | laminaribiose + 2 D-glucose | - |
? | |
| laminaritetraose + 2 H2O | from Laminaria digitata. HdLam33 performs a transglycosylation reaction transferring the laminaribiose unit in the non-reducing terminus of laminaritriose , as donor substrate, to a free laminaribiose, as acceptor substrate, giving laminaritetraose and glucose. The resulting laminaritetraose is subsequently hydrolyzed by HdLam33 into 2 mol of glucose and 1 mol of laminaribiose | Haliotis discus hannai | laminaribiose + 2 D-glucose | - |
? | |
| lichenan + H2O | from Cetraria islandica. HdLam33 produces no cellotriose, cellobiose, and glucose from lichenan, but produced the trisaccharide cellobiosyl-beta-1,3-glucose | Haliotis discus hannai | beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranose + ? | - |
? | |
| additional information | HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose | Haliotis discus hannai | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Haliotis discus hannai |
| More | HdLam33 primary structure, overview | Haliotis discus hannai |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-glucanase | - |
Haliotis discus hannai |
| HdLam33 | - |
Haliotis discus hannai |
| More | HdLam33 belongs to the glycoside hydrolase family 16, GH16 | Haliotis discus hannai |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Haliotis discus hannai |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified native enzyme, 15 min, loss of 50% activity | Haliotis discus hannai |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Haliotis discus hannai |
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Release 2023.1 (January 2023)
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