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Literature summary for 3.2.1.6 extracted from
- Highly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gen and characterization of the recombinant gene product (1997), Microbiology, 143, 1701-1708.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | disease protection of plants because of antimycotic activity in combination with chitinases | Thermotoga neapolitana |
General Stability
| General Stability | Organism |
|---|---|
| 30% of activity at 1% SDS w/v after 20 min incubation | Thermotoga neapolitana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermotoga neapolitana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
SDS-PAGE | Thermotoga neapolitana |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 40% of activity after 20 min incubation with 10% v/v ethanol | Thermotoga neapolitana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from recombinant Escherichia coli cells | Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Thermotoga neapolitana | D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose | laminaritriose | ? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,3-beta-glucanase | - |
Thermotoga neapolitana |
| laminarinase | - |
Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | 95 | - |
Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 100 | incubation at 100°C: 82% of initial activity at 95°C, 57% at 100°C | Thermotoga neapolitana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | 6.3 | - |
Thermotoga neapolitana |
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