Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant heterologous enzyme expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Theobroma cacao |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE | Theobroma cacao |
25580 | - |
x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE | Theobroma cacao |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Theobroma cacao | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Theobroma cacao |
Subunits | Comment | Organism |
---|---|---|
? | x * 25580, sequence calculation, x * 22000, recombinant enzyme, SDS-PAGE | Theobroma cacao |
monomer | the enzyme is a single chain protein presenting 7 alpha-helices, 10 beta-sheets and 4 salt bridges | Theobroma cacao |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Theobroma cacao |
TcGlu2 | - |
Theobroma cacao |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme has an active site of 13 amino acids typical of glucanase with beta-1,3 and 1,4 action mode, comparative molecular modelling, overview. The enzyme's active site is composed of 13 amino acids, Trp93, Leu94, Lys95, Asp96, His97, Gly98, Pro99, Asp100, Lys101, Gly102, Phe103, Glu104, Asp105, a space of 2831 A, and presents alpha-helices and loop structures | Theobroma cacao |
physiological function | the enzyme causes hydrolysis of the membrane of hyphae from Moniliophthora perniciosa. Recombinant beta-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa strain ALF553 mycelial growth | Theobroma cacao |