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Literature summary for 3.2.1.6 extracted from
- Production, purification and application-relevant characterisation of an endo-1,3(4)-beta-glucanase from Rhizomucor miehei (2007), Appl. Microbiol. Biotechnol., 76, 835-841.
General Stability
| General Stability | Organism |
|---|---|
| unaffected by pepsin, pancreatin and bile extract | Rhizomucor miehei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for beta-glucan is 1.87 mg/ml | Rhizomucor miehei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Rhizomucor miehei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39700 | - |
x * 39700, SDS-PAGE | Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | - |
DSM 1330 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizomucor miehei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | barley beta-glucan | Rhizomucor miehei | ? | - |
? |  |
| CM-curdlan + H2O | 80.7% of the activity with CM-curdlan | Rhizomucor miehei | ? | - |
? | |
| laminarin + H2O | 33.2% of the activity with CM-curdlan | Rhizomucor miehei | ? | - |
? | |
| lichenan + H2O | 67.27% of the activity with CM-curdlan | Rhizomucor miehei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39700, SDS-PAGE | Rhizomucor miehei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,3(4)-beta-glucanase | - |
Rhizomucor miehei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Rhizomucor miehei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | 40°C: 34% of maximal activity, 60°C: maximal activity | Rhizomucor miehei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 min, 4% loss of activity at high protein concentration | Rhizomucor miehei |
| 80 | - |
1 min, 6% loss of activity at high protein concentration | Rhizomucor miehei |
| 90 | - |
1 min, 19% loss of activity at high protein concentration | Rhizomucor miehei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15600 | - |
beta-Glucan | - |
Rhizomucor miehei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Rhizomucor miehei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | - |
Rhizomucor miehei |
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