Literature summary for 3.2.1.6 extracted from

Guo, Q.; Zhang, W.; Ma, L.L.; Chen, Q.H.; Chen, J.C.; Zhang, H.B.; Ruan, H.; He, G.Q.
A food-grade industrial arming yeast expressing beta-1,3-1,4-glucanase with enhanced thermal stability (2010), J. Zhejiang Univ. Sci. B, 11, 41-51.

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Application

Application	Comment	Organism
biotechnology	construction of a transgenic yeast, Saccharomyces cerevisiae, expressing the enzyme, results in a food-grade yeast that has the potential to improve the brewing properties of beer	Bacillus subtilis
food industry	construction of a transgenic yeast, Saccharomyces cerevisiae, expressing the enzyme, results in a food-grade yeast that has the potential to improve the brewing properties of beer	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
subcloning in Escherichia coli strain Dh5alpha, recombinant expression of beta-1,3-1,4-glucanase from Bacillus subtilis fused to alpha-agglutinin and expressed under the control of the GAL1 promoter in Saccharomyces cerevisiae, plasmid construction, overview	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant expression of beta-1,3-1,4-glucanase from Bacillus subtilis fused to alpha-agglutinin and expressed under the control of the GAL1 promoter in Saccharomyces cerevisiae to construct a food-grade industrial arming yeast displaying beta-1,3-1,4-glucanase on the cell surface and enzyme activity, and to evaluate the thermal stability of the glucanase for practical application	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	mutant strain ZJF-1A5	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-1,3-1,4-glucan + H2O	from Hordeum vulgare	Bacillus subtilis	?	-	?

Synonyms

Synonyms	Comment	Organism
beta-1,3-1,4-glucanase	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	free recombinant enzyme, 2 h, 51.9% remaining activity	Bacillus subtilis
60	-	free recombinant enzyme, 2 h, 20.1% remaining activity. Recombinant enzyme in transgenic Saccharomyces cerevisiae cells shows increased activity with incubation time and reaches 129.2% of activity compared to controls after 1 h, then the activity gradually decreases	Bacillus subtilis
70	-	free recombinant enzyme, 2 h, almost no remaining activity. Recombinant enzyme in transgenic Saccharomyces cerevisiae cells shows increased activity with incubation time and reaches 109.2% of activity compared to controls after 1 h, then the activity gradually decreases	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Bacillus subtilis

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Release 2023.1 (January 2023)